BR0013395A - Rapid method for measuring complex carbohydrates in mammalian tissue - Google Patents
Rapid method for measuring complex carbohydrates in mammalian tissueInfo
- Publication number
- BR0013395A BR0013395A BR0013395-7A BR0013395A BR0013395A BR 0013395 A BR0013395 A BR 0013395A BR 0013395 A BR0013395 A BR 0013395A BR 0013395 A BR0013395 A BR 0013395A
- Authority
- BR
- Brazil
- Prior art keywords
- complex carbohydrates
- mammalian tissue
- measuring complex
- slurry
- measuring
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 6
- 150000001720 carbohydrates Chemical class 0.000 title abstract 3
- 235000014633 carbohydrates Nutrition 0.000 title abstract 3
- 239000002002 slurry Substances 0.000 abstract 3
- 230000007062 hydrolysis Effects 0.000 abstract 2
- 238000006460 hydrolysis reaction Methods 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 229920002527 Glycogen Polymers 0.000 abstract 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 229940096919 glycogen Drugs 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
"MéTODO RáPIDO PARA MEDIçãO DE CARBOIDRATOS COMPLEXOS EM TECIDO DE MAMìFERO". A invenção descreve um método rápido de medição de carboidratos complexos em tecido de mamífero, o dito método compreendendo as etapas de: extração de uma amostra de tecido a ser testada; formação de uma pasta fluida homogênea da amostra com uma solução aquosa; adição de enzima de hidrólise suficiente para assegurar hidrólise completa de glicogênio na pasta fluida; e medição da concentração de glicose na pasta fluida. O método pode ser realizado pós-mortem para avaliar a concentração do momento da matança. A invenção descreve ainda a medição de concentrações de lactato. O método da invenção pode ser realizado em trinta minutos ou menos."QUICK METHOD FOR MEASURING COMPLEX CARBOHYDRATES IN MAMMALIAN TISSUE". The invention describes a rapid method of measuring complex carbohydrates in mammalian tissue, said method comprising the steps of: extracting a tissue sample to be tested; formation of a homogeneous slurry of the sample with an aqueous solution; addition of sufficient hydrolysis enzyme to ensure complete hydrolysis of glycogen in the slurry; and measuring the concentration of glucose in the slurry. The method can be carried out post-mortem to assess the concentration at the time of killing. The invention further describes the measurement of lactate concentrations. The method of the invention can be carried out in thirty minutes or less.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NZ33727699 | 1999-08-17 | ||
| PCT/NZ2000/000159 WO2001012844A1 (en) | 1999-08-17 | 2000-08-17 | Rapid method for measuring complex carbohydrates in mammalian tissue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0013395A true BR0013395A (en) | 2002-07-09 |
Family
ID=19927450
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0013395-7A BR0013395A (en) | 1999-08-17 | 2000-08-17 | Rapid method for measuring complex carbohydrates in mammalian tissue |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1210451A4 (en) |
| AR (1) | AR025303A1 (en) |
| AU (1) | AU779415C (en) |
| BR (1) | BR0013395A (en) |
| CA (1) | CA2382155A1 (en) |
| WO (1) | WO2001012844A1 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0218868D0 (en) | 2002-08-14 | 2002-09-25 | Nasir Muhammed A | Improved airway management device |
| GB2555360B (en) | 2013-12-17 | 2018-10-10 | Intersurgical Ag | Intubating Airway Device |
| USD1051359S1 (en) | 2015-06-15 | 2024-11-12 | Intersurgical Ag | Airway device |
| USD842456S1 (en) | 2015-12-15 | 2019-03-05 | Intersurgical Ag | Airway device |
| GB201720733D0 (en) | 2017-12-13 | 2018-01-24 | Ashkal Development Ltd | Airway device |
| JP1649724S (en) | 2019-01-18 | 2020-01-14 | ||
| USD1025348S1 (en) | 2020-04-16 | 2024-04-30 | Intersurgical Ag | Airway device |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4467811A (en) * | 1979-08-02 | 1984-08-28 | Children's Hospital Medical Center | Method of polarographic analysis of lactic acid and lactate |
| US5262305A (en) * | 1991-03-04 | 1993-11-16 | E. Heller & Company | Interferant eliminating biosensors |
-
2000
- 2000-08-17 BR BR0013395-7A patent/BR0013395A/en not_active IP Right Cessation
- 2000-08-17 CA CA002382155A patent/CA2382155A1/en not_active Abandoned
- 2000-08-17 WO PCT/NZ2000/000159 patent/WO2001012844A1/en not_active Ceased
- 2000-08-17 AR ARP000104247 patent/AR025303A1/en unknown
- 2000-08-17 AU AU67425/00A patent/AU779415C/en not_active Ceased
- 2000-08-17 EP EP00955193A patent/EP1210451A4/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP1210451A4 (en) | 2004-08-25 |
| AR025303A1 (en) | 2002-11-20 |
| AU779415C (en) | 2006-02-09 |
| AU6742500A (en) | 2001-03-13 |
| EP1210451A1 (en) | 2002-06-05 |
| AU779415B2 (en) | 2005-01-20 |
| WO2001012844A1 (en) | 2001-02-22 |
| CA2382155A1 (en) | 2001-02-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application fees: dismissal - article 86 of industrial property law |
Free format text: REFERENTE A 6A, 7A E 8A ANUIDADES. |
|
| B08K | Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87) |
Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1962 DE 12/08/2008. |