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BR0012856A - Recombinant Aspergillus soybean, methods for introducing a nucleic acid sequence into aspergillus soybean, to select transformed or transfected aspergillus soybean, to produce recombinant aspergillus soybean, mutant or recombinant aspergillus soybean, recombinant fungus, expression of an introduced nucleic acid sequence, for the expression of a protein or polypeptide, for the production of a protein or polypeptide, and, to produce a phytase or a protein having phytase activity - Google Patents

Recombinant Aspergillus soybean, methods for introducing a nucleic acid sequence into aspergillus soybean, to select transformed or transfected aspergillus soybean, to produce recombinant aspergillus soybean, mutant or recombinant aspergillus soybean, recombinant fungus, expression of an introduced nucleic acid sequence, for the expression of a protein or polypeptide, for the production of a protein or polypeptide, and, to produce a phytase or a protein having phytase activity

Info

Publication number
BR0012856A
BR0012856A BR0012856-2A BR0012856A BR0012856A BR 0012856 A BR0012856 A BR 0012856A BR 0012856 A BR0012856 A BR 0012856A BR 0012856 A BR0012856 A BR 0012856A
Authority
BR
Brazil
Prior art keywords
aspergillus
soybean
protein
recombinant
polypeptide
Prior art date
Application number
BR0012856-2A
Other languages
Portuguese (pt)
Inventor
Margreet Heerikhuisen
Cornelis Antonius Maria Hondel
Peter Jan Punt
Nicolaas Adriaan Van Biezen
Alwines Rene Albers
Kurt Vogel
Original Assignee
Tno
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tno, Hoffmann La Roche filed Critical Tno
Publication of BR0012856A publication Critical patent/BR0012856A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"ASPERGILLUS SOJAE RECOMBINANTE, MéTODOS PARA INTRODUZIR UMA SEQUêNCIA DE áCIDO NUCLEICO EM ASPERGILLUS SOJAE, PARA SELECIONAR ASPERGILLUS SOJAE TRANSFORMADO OU TRANSFECTADO, PARA PRODUZIR ASPERGILLUS SOJAE RECOMBINANTE, ASPERGILLUS SOJAE MUTANTE OU RECOMBINANTE, FUNGO RECOMBINANTE PROCESSOS DE EXPRESSãO DE UMA SEQuêNCIA DE áCIDO, NUCLEICO INTRODUZIDA, PARA A EXPRESSãO DE UMA PROTEìNA OU POLIPEPTìDEO, PARA A PRODUçãO DE UMA PROTEìNA OU POLIPEPTìDEO, E, PARA PRODUZIR UMA FITASE OU UMA PROTEìNA TENDO ATIVIDADE FITASE". Aspergillus sojae recombinante compreendendo um gene acetamidase S ( amdS ) introduzido como marcador selecionável é descrito. Um Aspergillus sojae demonstrando crescimento com meio compreendendo uracila e ácido fluoro-orótico, referido Aspergillus sojae ainda não demonstrando crescimento em meio compreendendo uridina e ácido fluoro-orótico, isto é, referido Aspergillus sojae demonstrando auxotrofia uracila, referido Aspergillus sojae sendo incapaz de utilizar uridina, referido Aspergillus sojae sendo negativo para pyrG, referido Aspergillus sojae demonstrando resistência a ácido fluoro-orótico, referida auxotrofia a uracila e referida resistência a ácido fluoro-orótico sendo reparáveis quando da complementação com um gene pyrG ativo introduzido, é descrito. Aspergillus sojae ainda compreende uma seq³ência de ácido nucleico codificando uma fitase ou uma proteína tendo atividade fitase ou qualquer outra proteína heteróloga ou polipeptídeo e pode ser usado para a produção biotecnológica de referida fitase ou referida outras proteínas ou polipeptídeos heterólogos. Os mutantes adicionais demonstrando morfologia emendada são também descritos. Os métodos para produzir estes hospedeiros de expressão são descritos."ASPERGILLUS SOJAE RECOMBINANTE, METHODS FOR INTRODUCING A NUCLEIC ACID SEQUENCE IN ASPERGILLUS SOJAE, TO SELECT ASPERGILLUS SOJAE TRANSFORMED OR TRANSFECTED, TO PRODUCE ASPERGILLUS SOJAE RECOMBINANT, ASPERGILLUS SOUTH, SOUTH EMERGENCY, SOUTH EMERGING , FOR THE EXPRESSION OF A PROTEIN OR POLYPEPTIDE, FOR THE PRODUCTION OF A PROTEIN OR POLYPEPTIDE, AND, TO PRODUCE A PHYTASE OR A PROTEIN HAVING PHYTASE ACTIVITY ". Recombinant Aspergillus soybean comprising an acetamidase S (amdS) gene introduced as a selectable marker is described. An Aspergillus soybean showing growth with medium comprising uracil and fluoro-orotic acid, referred to Aspergillus soybean and not yet showing growth in medium comprising uridine and fluoro-orotic acid, ie, referred to Aspergillus soybean showing uracil auxotrophy, referred to as Aspergillus soy and being unable to use uridine. , referred to Aspergillusoyae being negative for pyrG, referred to Aspergillusoyae showing resistance to fluoro-orotic acid, referred to uracil auxotrophy and referred to resistance to fluoro-orotic acid being repairable when complemented with an active pyrG gene introduced, is described. Aspergillus soybean further comprises a nucleic acid sequence encoding a phytase or a protein having phytase activity or any other heterologous protein or polypeptide and can be used for the biotechnological production of said phytase or said other heterologous proteins or polypeptides. Additional mutants showing spliced morphology are also described. Methods for producing these expression hosts are described.

BR0012856-2A 1999-07-30 2000-07-28 Recombinant Aspergillus soybean, methods for introducing a nucleic acid sequence into aspergillus soybean, to select transformed or transfected aspergillus soybean, to produce recombinant aspergillus soybean, mutant or recombinant aspergillus soybean, recombinant fungus, expression of an introduced nucleic acid sequence, for the expression of a protein or polypeptide, for the production of a protein or polypeptide, and, to produce a phytase or a protein having phytase activity BR0012856A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP99202516 1999-07-30
EP99202517 1999-07-30
PCT/NL2000/000544 WO2001009352A2 (en) 1999-07-30 2000-07-28 Use of aspergillus sojae as host for recombinant protein production

Publications (1)

Publication Number Publication Date
BR0012856A true BR0012856A (en) 2002-06-11

Family

ID=26153350

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0012856-2A BR0012856A (en) 1999-07-30 2000-07-28 Recombinant Aspergillus soybean, methods for introducing a nucleic acid sequence into aspergillus soybean, to select transformed or transfected aspergillus soybean, to produce recombinant aspergillus soybean, mutant or recombinant aspergillus soybean, recombinant fungus, expression of an introduced nucleic acid sequence, for the expression of a protein or polypeptide, for the production of a protein or polypeptide, and, to produce a phytase or a protein having phytase activity

Country Status (10)

Country Link
EP (1) EP1204758A2 (en)
JP (1) JP2003518921A (en)
KR (1) KR20020059350A (en)
CN (1) CN1369016A (en)
AR (1) AR024989A1 (en)
AU (1) AU782116B2 (en)
BR (1) BR0012856A (en)
CA (1) CA2378016A1 (en)
MX (1) MXPA02001061A (en)
WO (1) WO2001009352A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US7122330B2 (en) 2000-04-13 2006-10-17 Mark Aaron Emalfarb High-throughput screening of expressed DNA libraries in filamentous fungi
US8551751B2 (en) 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
JP2011239681A (en) * 2008-09-19 2011-12-01 Kikkoman Corp Koji mold alkaline protease promoter
CN101503660B (en) * 2009-02-10 2011-07-27 汪兵 Engineering bacteria expressing thermophilic saccharifying enzyme and use thereof
BR112013025430A2 (en) 2011-04-22 2016-11-29 Danisco Us Inc "Variant strains of filamentous bottoms having genetic modification for increased mpg1 protein production and altered growth and their production method."
US9587242B2 (en) * 2011-04-22 2017-03-07 Danisco Us Inc. Filamentous fungi having an altered viscosity phenotype

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2748347B2 (en) * 1989-03-09 1998-05-06 キッコーマン 株式会社 Aspergillus soya transformant
DE19526485A1 (en) * 1995-07-20 1997-01-23 Roehm Gmbh Recombinantly produced leucine aminopeptidase from Aspergillus sojae
JPH11501217A (en) * 1995-08-03 1999-02-02 ギスト ブロカデス ベスローテン フェンノートシャップ Use of the homologous amdS gene as a selectable marker
JP3727780B2 (en) * 1998-06-12 2005-12-14 キッコーマン株式会社 Leucine aminopeptidase gene, recombinant DNA, and method for producing leucine aminopeptidase

Also Published As

Publication number Publication date
JP2003518921A (en) 2003-06-17
CN1369016A (en) 2002-09-11
AR024989A1 (en) 2002-11-06
WO2001009352A3 (en) 2001-11-08
KR20020059350A (en) 2002-07-12
EP1204758A2 (en) 2002-05-15
MXPA02001061A (en) 2003-04-10
WO2001009352A2 (en) 2001-02-08
AU782116B2 (en) 2005-07-07
CA2378016A1 (en) 2001-02-08
AU6323800A (en) 2001-02-19

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Legal Events

Date Code Title Description
B08F Application fees: application dismissed [chapter 8.6 patent gazette]

Free format text: REFERENTE A 5A, 6A, E 7A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1929 DE 26/12/2007.