BG66186B1 - Biologically active food supplement and a method of its preparation - Google Patents

Abstract

The biologically active food supplement (BAFS) aims at improving self-regulation in the human body, at assisting the prophylaxis and enhancing the immune system, at boosting a personÆs energy levels.The method for obtaining the primary substance includes gathering liquid from the mammary glands of mammals such as different breeds of cows, female buffaloes, ewes, she-goats, mares or female donkeys û 48 h before giving birth (the period is different for the different animals) and 72 h after giving birth. After BAFS intake, the body is provided in a natural way with ready antibodies and immunoglobulins which activate the B-lymphocytes, the humoral immune response, and the specific immune defence. The lactoferrin, the proline rich (PRP) polypeptide, the cytokines such as interleukins 1, 6, 10, the Interferon-gamma, etc. contained in the supplement regulate the thymus glandÆs function, activate the T-lymphocytes and the Th-1 cells and stimulate the cellular immune response and the cellular immune defence. Self-regulation in the body is assisted by the transforming growth factors (TGF-A, TGF-B), the growth hormone (hGH), oxytocin and prolactin contained in the preparation and most of all û by the cytokines which contain info-molecules and regenerate muscles, tissues, bones, neurocells, correct the genetic makeup and reach to DNA and RNA, thus creating infinite opportunities for elevating vitality and lengthening the life expectancy.

Description

(54) BIOLOGICAL ACTIVE NUTRITION SUPPLEMENT (BAHD) AND METHOD OF PREPARING IT

Technical field

The invention relates to a biologically active food supplement and a method for its preparation, which accelerates self-regulation in the body, improves human immunity by using unconventional approaches, strengthening links with the universe and healthy lifestyles. A new immune stimulant and modulator will take its place in the prevention and improvement of the immune system, supplying the body with antibodies (immunoglobulins) and assisting in the treatment of immune diseases, mainly related to specific immune modulation and humoral immune responses. Many ingredients of the preparation, such as lactoferrin, a proline-rich polypeptide (PRP), cytokines, etc., stabilize the thymus and activate T-leukocytes and Th--cells, which stimulates the cellular immune response and cellular immune protection.

The ability of the human body to regenerate tissues, restore lost energy, counteract the loss of elasticity of the skin and muscle mobility is gradually diminished, and the body must be assisted in activating the processes of self-regulation, increasing the protective forces and prolonging the duration life.

This is also a breakthrough in the fight against cancer and related AIDS diseases. Growth factors and growth hormone, oxytocin, prolactin and most of all the cytokines contained in the preparation are of particular importance for enhancing the immunity of young organisms and at the same time info-regenerate tissues, muscles, bones, nerves, skin in the elderly and discover non-exhaustive Reserves to extend life expectancy by entering the genetic program and activating human primary immunity. This is mainly done through cytokines such as interferon 1,6,10, gamma interferon and others and the "smart" molecules contained therein.

BACKGROUND OF THE INVENTION

R PPLKTM1GYA BREASTS. THESE BREEDS are commercially available as biologically active substances for food, which are recommended to improve the immune system, such as echinacea, cordyceps, cat nail, spirulina and others, mainly on a plant-based basis. The disadvantage of these products, also experimented by the authors, is that they have to be applied for a long time. This led the authors to turn to other compositions of animal origin and to look for mechanisms closest to those in the human body.

Known are foodstuffs biological substances to stimulate the immune system, suppressing infection and the treatment and recovery of tissues containing colostrum, lactoferrin and modified citrus pectin. Food additive main component colostrum performed immunostimulatory function, it contains immunoglobulins classes IgA, IgG, IGM, as well as growth factors (IGI-1, TGF-A, TGF-B). Colostrum supplies the body with a proline-rich polypeptide that models the activity of the immune system by acting on the thymus and determining the tolerance of immune cells, i. when to stop the fight against invaders in the body. Colostrum ingredients provide growth and repair factors for tissues, muscles, bones, cartilage and nerves. The modified fruit peptide provides the composition with additional benefits for the nutritional supplement. Citrus pectin contains protein molecules that have a binder for some types of cancer (US 6 780 438 B2). The difference between this patent and the claimed one consists in several points: 1. The technology of production of the two products is radically different. The product according to the invention creates conditions for the absorption of 99% in the body due to the atomization of the particles. In this invention, the absorption of the product in the body is no more than 10-15%. In contrast to this patent in the invention, all the components are of natural animal origin. In doing so, a large amount of glucose is avoided - by right-handed rotation of the torsion field. Proteins, respectively amino acids and colostrum, have left-sided torso rotation. In products with right-sided rotation (glucose) and left-sided rotation (amino acids), the bio-pulsations of cells in the body disrupt their

66186 Bl dark and harming the body (US 6780438 and US 2005/0025837 A1).

Known is also composition comprising colostrum and / or colostrum extracts in solid, liquid, pasty or microencapsulated form, minerals or mineral salts from the Dead Sea, plant components and / or plant extracts. The compositions are used as cosmetics, nutritional supplements and medicaments, in particular for the prevention and treatment of skin diseases and as immunostimulants (EP 1 180 940).

It is known document which refers to a composition for use in topical treatment of skin diseases and lesions containing equine colostrum or a mixture of horse colostrum and horse milk in a ratio of from 1:10 to 3: 1 by weight of the composition (US 5,750,149 ).

A method is known for preparing a cow and sheep colostrum powder comprising the steps of colostrum collection, sterilization, concentration, freeze-drying and crushing. The cow and sheep colostrum powder produced is a highly effective product that contains a large amount of immunoglobulin, which is long-lasting and has a uniform structure and good taste. The information in this patent is insufficient and lists only the stages of preparation and production of colostrum - dust. Traditional cryo-technology and "crushing" are used as a mechanical process where high atomization of the product particles cannot be achieved (CN 167 663).

SUMMARY OF THE INVENTION

The essence of the invention is to provide a biologically active nutritional supplement containing material from a mammalian female animal of one species of cow, sheep, goat, buffalo, mare or donkey, this material being the substance separated from the mammary glands 48 hours before birth and 72 h after birth, treated by deep freezing and nebulization, the main components being 25-30% immunoglobulins in 200 mg IG, epithelial tissue growth factors, insulin-like factor, lactoferrin, cytokines, vitamins, alkaline earth metals, enzymes , Oligosaccharides, a polypeptide rich in proline and the amino acids of trypsin, leucine, valine, lysine, alanine and methionine, oligosaccharides laktalalbumin, prolactin and oxytocin and additional substances.

The dietary supplement contains the additional substances glycoproteins - trypsin inhibitor and protease to protect the gastric mucosa.

The dietary supplement may contain additional substances such as yoghurt enriched with probiotics: L. Bulgaricus, S. Thermophilus, L. Acidophilus, Bifidusbacterium, L. Casei cultures and the ratio between the main ingredient and yogurt is 97%: 3% or 95%: 5% or 90%: 10%.

The biologically active nutritional supplement may contain additional substances, such as fruit mixture of peach, orange, strawberry or other in a weight ratio of 99%: 1%.

The dietary supplement may contain the additional substance collagen H, with a weight ratio of 15%: 85%. Biologically active food additive can be enriched with powders of natural products containing useful alkaline earth metals, mainly calcium carbonate and small amounts of magnesium, iron, phosphorus and sulfur compounds.

The method according to the invention comprises as a first step the collection of a thick yellowish substance (48 h prior to preparation of uterine activity - a period different for different animals and 72 h after the birth) in cows, buffalos, goats, sheep, mares, and she asses. It would be best to select the location of farms in ecologically clean areas at the foot of the mountains - Stara Planina, Sredna Gora, Rhodopes and others. Collection of primary material must be carried out by refrigerated vehicles.

The collection of primary material requires extremely high hygiene, with better results obtained with the use of sterile milking machines and automatic milking than with manual milking. Specific measures should lead to an entry in the European standards for microbial contamination such as total number of microorganisms (CFU / g), mushrooms (CFU / g), E-coli, Solmonella, P. Aerodinoza and others (Annex 1a).

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The collection period for a quality substance is limited in time and must be controlled. After 72 hours after birth, the quality of the product changes dramatically. As a rule, cows gather about 631 for 72 hours, sucking their calves from their mother. The highest content of immunoglobulins occurs in the first two days after birth.

The next step is purification, which involves degreasing and removing lactose, which is in insignificant amounts of 8 10%.

After degreasing, the yellowish color of the substance turns pale yellow. A further step of the process according to the invention is deep freezing of the primary product, which is carried out at very low temperatures. The low temperature does not allow the proteins to be denatured and, above all, retains passive antibodies - immunoglobulins. Lyophilization is then carried out by means of modern freezing systems - low temperature thawing and product spraying.

The main component of the compositions according to the invention is a natural thick yellowish substance. The substance contains 25-30% of immunoglobulins in 200 mg IG. In fact, these are ready-made antibodies that exert specific immunomodulation (Annexes 1a, 16, 1c and 2).

The nutritional supplement contains several transforming growth factors, the epithelial tissue growth factor - IGF, which is associated with wound healing, ulcers, and strengthens the mucous membranes of all systems (insulin-like factor - IGF-1 plays an important role in regulating blood sugar and cholesterol, metabolism and gastrointestinal function; IGF-1 restores DNA and RNA (Appendix 3).

Transforming growth factors TGF A and TGF B are responsible for cartilage and bone formation.

The growth hormone (H.GH) contained in the supplement regulates growth in young organisms and improves immunity. Growth factors, growth hormone, and in particular cytokines, restore muscle, tendons, bones, nerve cells, tissues, skin, thereby infecting older organisms; transmit information to cells together with the hormones of the endocrine glands, this information reaching the level of DNA and RNA recovery; rejuvenation reserves and longer life expectancy and longevity are opened. The content of prolactin, a breastfeeding, oxytocin-stimulating for delivery, is of particular importance for young organisms (See Appendix 3 for the main components and their effects on the human body). Lactoferrin (a strong antiviral and antibacterial agent) and cytokines (Interleukins 1,6,10, gamma-interferon, etc.) activate T-lymphocytes and Th-1 cells, which are associated with the fight against cancer and related AIDS diseases. Proline-rich polypeptide (PRP) regulates thymus function and cell tolerance. The last three ingredients (lactoferrin, cytokines and proline-rich polypeptide) stimulate cellular immune response and cellular immune defenses, including tolerance cells - when to stop the attackers (Annex 3) Vitamins (especially in group B, A, D ), alkaline earth metals (Ca, U, Mg.), enzymes, amino acids (proline, leucine, valine, lysine, alanine, methionine, etc.), oligosaccharides (protect the stomach walls from harmful germs), lacto albumins (stimulate secretion) of serotonin) and others, are of particular importance in metabolism, improving gastrointestinal tract, involved in the fight against multiple sclerosis, chronic fatigue syndrome, scleroderma, arthritis and osteoporosis (Annexes 1a, 2 and 3).

The sulfur compounds contained in BAHD, among which methyl sulfonyl methane (MSM), makes the product indispensable for arthritis, osteoporosis, etc., which has led to the idea of creating an arthro-formula in combination with other ingredients such as Collagen H, alkaline earth metals and others.

Studies have shown that the powdery substance obtained according to the invention has the ability to:

- to kill bacteria and viruses;

- to enrich the immune system with finished antibodies;

- stimulate the tissues to renew;

- increase the body's resistance forces;

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- improve the intestinal flora and gastrointestinal tract;

- stimulate the pituitary gland in the release of growth hormone, prolactin, oxytocin, etc. ·;

- stimulate through the pituitary gland - hormones of the thyroid gland, of the adrenal glands, of the pancreas, of the glands;

- stimulate neuropeptides in the pituitary-hypothalamic region;

- to support tissues and all systems in the body through growth factors by opening a new page to extend life expectancy and longevity;

- to support the thymus gland to activate Th-Ι cells and stimulate the cellular immune response, as well as in the fight against cancer and related AIDS diseases;

- a powerful antioxidant, healing wounds, fighting tumor and cancer cells;

- rescuer from chronic fatigue syndrome, multiple sclerosis, scleroderma, etc .;

- improves the absorption of food from the intestine, increases metabolism, strengthens muscles, increases the levels of glucose in the blood and brain.

The results of application of the preparation include a sharp increase in energy in the body. (Applications 4.1.a 4.1.6,4.2.a and 4.2.6 with the original experiment, where it is seen that the rate of activation as the main characteristic of energy after a two-month administration of BAHD, has increased from 0.92 to 3, 31 pixels and is in the normal range of 2.0 - 4.0 pixels).

The mechanism of action of the immune modulator - BAHD acts on the human body through the blood flow that enters the blood depots, among which are the lymph nodes and the spleen. In these depots, B-lymphocytes accumulate, which form antibodies and exert specific immunomodulation and humoral immune responses in the body.

Maternity glands contain all five classes of immunoregulators. Immunoglobulin IgM are the antibodies that first respond to infection; IgG are antibodies that make up 70% of all antibodies and act through the bloodstream, plasma and tissues (Annexes 1a, 16, 1c and 2).

Ig.A are antibodies that appear on the surface of the lungs and intestines and with their secretions affect the bronchi and intestines (Annexes 1a and 1b). IgE are antibodies that are associated with allergic reactions; IgDs are antibodies that are located on the surface of B cells (Annex 2).

The use of the biologically active nutritional supplement is related to: 1. the effect of blood flow; 2. with a sharp improvement of the gastrointestinal tract and intestinal flora; 3. with renewal and repair of tissues and muscles, bones, nerves; 4. a sharp increase in energy; improving the body's resistance and resilience; 5. the key to longevity; 6. improving the specific immunomodulation and humoral immune response in the body; 7. activation of the thymus, T-lymphocytes and Th-те cells, which complement the range of natural immunomodulators in the fight against free radicals, diabetes, cancer and similar diseases of AIDS through activation of the cellular immune response in the body and cellular immune protection.

The content of growth hormone and a number of transforming growth factors, prolactin, oxytocin and more. It is associated with pituitary-hypothalamic area, through which is regulated impact hormones and neuropeptides throughout the body and stimulate the young organism and increases energy, vitality and efficiency of adults (Appendix 3). Thanks to the cytokines contained in the BAHD, self-regulation is carried out in the human body, the automatic functions of the immune system are realized, the automatic interaction between the immune, nervous and endocrine systems and ultimately DNA and RNA and the human genetic program are corrected. All self-regulatory processes in man are a gift from the mother universe and have existed for thousands of years before the advent of medicine and pharmacy. The human body is "the best hospital and pharmacy".

Examples of carrying out the invention

Example 1. Biologically active food supplement (BAHD) prepared according to the method

66 186 Bl of the invention is a pale yellowish powder of substance of dairy animals 48 h before birth (within different in different species) and 72 h after the birth, which may be enriched with yogurt and probiotics. This natural formula supplies the body with antibodies (against more than 20 types of invaders); increases the body's resistance, dramatically increases the body's energy and vitality, and also improves digestion and metabolism; has a positive effect on the intestines, tissues, muscles, bones, nerves and skin. It should be noted that these are active yogurt containing probiotics, such as L. Bulgaricus, S. Thermophilus, Bifidobacterium, L. Acidophilus and L. Cassei cultures. The ratio of basic substance to yogurt is in three variants: 97% - BAHD and 3% - yogurt; 95% - BAHD and 5% - yogurt; 90% - BAHD and 10% yogurt.

Ingredients: immunoglobulins, transforming growth factors, growth hormone, proline-rich polypeptide, amino acids (trypsin, leucine, valine, lysine, alanine, etc., which are a wonderful food for the body and supply it with the eight essential amino acids; bifidobacterium, lactobacillus, acidophilus, etc. (described in detail above), probiotics help the intestinal flora of the gastrointestinal tract and of all processes in the body; alkaline earth metals (Ca, K, P, Se, Zn, Cr), etc. complete the wide range of benefits for the body.

Example 2. The biologically active food additive (BAHD) obtained according to the method of the invention is a pale yellowish powder from the substance of the dairy animal (48 h before birth and 72 h after birth) enriched with fruit mixtures - peach, cherry, orange, strawberry and others.

The content of the fruit mixes is 1% - mainly to improve the taste.

The results are the same as Example 1, this substance being for children.

Ingredients: immunoglobulins, prolactin, oxytocin, growth hormone, transforming growth factors, polypeptide, proline-rich, lactoferrin, cytokines, oligosaccharides, amino acids, alkaline earth metals (Ca, K, Se, P and S compounds, etc.) others. With product - example

2, as in example 1, both the specific immunomodulation and the humoral immune response (See Appendix 2) and the cellular immune defense are carried out (see

Annex 3).

Example 3. The biologically active food supplement (BAHD) obtained according to the method of the invention is a pale yellowish powder from the substance of the dairy animal 48 h before birth and 72 h after birth mixed with Collagen H - bone extract from young (no more than 1.5 years) cattle in proportion - BAHD main substance - 15% and Collagen H - 85%. Combining BAHD with Collagen H produces a powerful antioxidant that allows for lasting results, such as increasing endurance and performance and creating stability and comfort for the body. The main purpose of this product is to combine the properties of BAHD (described above) with the properties of collagen N. It contains 96.2% amino acids in 100% mineral, supplying the body with essential acids such as proline, alanine, glutamic acid, aspartic acid, valine, arginine, lysine, leucine, glycine, serine, tyrosine, collagen H contains 1.5% alkaline earth metals such as calcium, magnesium, potassium, sodium, iron, zinc, phosphorus compounds.

Contents: immunoglobulins, oxytocin, prolactin, growth factors, proline-rich polypeptide, amino acids (described above); alkaline earth metals (Ca, Mg, P-compounds, methyl sulfanyl methane - MSM and others).

The product of Example 3 has a strong action as a strong antioxidant, it acts on metabolism, but its main purpose is to accumulate collagen H in bones and cartilage, to overcome inflammation and arthritic pain, to increase calcium content, to regulate the balance between calcium - magnesium and together with the sulfur compounds - MSM in BAHD to form a strong anti-inflammatory substance to remove disease states in the bones, muscles, tendons and tissues.

Example 4. Biologically active food additive (BAHD), a pale yellowish powder from the substance of dairy animals 48 hours before birth and 72 hours after birth, enriched with powders of natural natural products containing alkaline earth metals according to the original idea.

The ratio of BAHD to alkaline earth metals is in the following parts by weight: BAHD -150 parts (83.33%), calcium carbonate - 20 parts (11.11%), other alkaline earth metals (magnesium, iron, phosphorus and sulfur compounds -10 parts (5.57%). The purpose of example 4 is to make a arthro-formula that combines the properties of BAHD (described above), and particularly the content of sulfur compounds - methyl sulfanyl methane (MSM), the construction of the cartilage, connective tissues, growth factors such as TGF-B contained in BAHD and alkaline earth metals, affecting density and healthy bone marrow, as well as the "smart" molecules of cytokines and their informational role in self-regulation in the human body.

Powdered preparations may be dissolved in water or milk or added to food and must be taken in the fasting state. Single administration is one teaspoon twice daily for adults.

In babies (6 months to 1 year old), 1/4 teaspoon is given per day; in children from 1 to 5 years 1/2 teaspoon daily; 1/2 to 6 teaspoons are given twice a day for children 6-12 years old.

The powder can be taken with yogurt, and the one with fruit mixtures dissolved in water or juice. For therapeutic purposes, dosages may be doubled in the elderly. The preparation is harmless.

BAHD with various combinations can also be produced in capsules containing the powder.

Claims (6)

Claims 1. A biologically active food additive (BAHD) in the form of a powder containing a yellowish liquid from a mammalian female animal of one of the cows, sheep, goats, buffaloes, mares or donkeys, characterized in that it is a substance separated from milk glands 48 h before birth and 72 h after birth treated with degreasing, freezing, drying and pulverizing, the main components being 25-30% immunoglobulins in 200 mg IG, transforming growth factors, growth hormone, epithelial tissue factor, insulin-like factor, l caffeine, information-regenerating cytokines such as 1,6,10 interleukins, gamma-interferon, vitamins, alkaline earth metals, enzymes, oligosaccharides, polypeptide, rich in proline and amino acids - trypsin, leucine, valine, lysine, alanine, metilin and alanine prolactin, oxytocin and additives. The biologically active nutritional supplement according to claim 1, characterized in that the additional substances are pcoproteins - trypsin inhibitor and protease. Biologically active food supplement according to claim 1, characterized in that the additional substances are yogurt containing the probiotics L. bulgaricus, S. thermophilus, L. acidophilus, bifidusbacterium, L. casei cultures and the ratio between the basic ingredient and the acidic milk is 97%: 3% or 95%: 5% or 90%: 10%. 4. The biologically active food additive according to claim 1, characterized in that said complementary substances are fructose mixture of peach, orange, strawberry or other in a weight ratio BAHD to fructose mixture as 99%: 1%. 5. The dietary supplement according to claim 1, characterized in that the additional substance is collagen H, the weight ratio being a major constituent of BAHD 15%: 85% collagen H. 6. A method of producing a biologically active food additive in powder form according to claim 1 from a mammalian female animal 48 hours before birth and 72 hours after the birth of an animal of one of the cows, sheep, goats, buffaloes, mares or donkeys, characterized by with degreasing, freezing to low temperatures, drying below -45 ° C and atomization.