BG110045A - Biologically active food supplement and a method of its preparation - Google Patents

Abstract

The biologically active food supplement (BAFS) aims at improving self-regulation in the human body, at assisting the prophylaxis and enhancing the immune system, at boosting a personÆs energy levels.The method for obtaining the primary substance includes gathering liquid from the mammary glands of mammals such as different breeds of cows, female buffaloes, ewes, she-goats, mares or female donkeys û 48 h before giving birth (the period is different for the different animals) and 72 h after giving birth. After BAFS intake, the body is provided in a natural way with ready antibodies and immunoglobulins which activate the B-lymphocytes, the humoral immune response, and the specific immune defence. The lactoferrin, the proline rich (PRP) polypeptide, the cytokines such as interleukins 1, 6, 10, the Interferon-gamma, etc. contained in the supplement regulate the thymus glandÆs function, activate the T-lymphocytes and the Th-1 cells and stimulate the cellular immune response and the cellular immune defence. Self-regulation in the body is assisted by the transforming growth factors (TGF-A, TGF-B), the growth hormone (hGH), oxytocin and prolactin contained in the preparation and most of all û by the cytokines which contain info-molecules and regenerate muscles, tissues, bones, neurocells, correct the genetic makeup and reach to DNA and RNA, thus creating infinite opportunities for elevating vitality and lengthening the life expectancy.

Description

Name:

IMMUNEX METHOD AND COMPOSITIONS

Sofia, January 30, 2008 • ·

Content of the Invention

IMMUNEX METHOD AND COMPOSITIONS

Pages

Research Papers1

Technical field 1

BACKGROUND OF THE INVENTION 2

Technical nature2

EXAMPLES 7

Claims 9

ANNEX 1a - Analytical protocol of Immunex assay for the presence of immunoglobulins proteins and microbial numbers (Laboratory study of BULBIO Ltd. - Sofia)

ANNEX 16- Results of Immunex Immunoglobulin Class A Ig (Laboratory Study of BULBIO Ltd. - Sofia)

ANNEX 1c Immunex test results for the presence of IgG immunoglobulins (BULBIO Laboratory Study - Sofia)

ANNEX 2 Mechanism for Exposure to Biologically Active Supplements (BAD) - Immunex by Specific Immunomodulation

ANNEX 3 Effect of Immunex content on cellular immune protection and self-regulation in the body

ANNEX 4.1 .a. Study of a 71-year-old woman before Imunex administration

ANNEX 4.1.6. General health indicators, incl. Activation factor before using Imunex

ANNEX 4.2.a. Study of a 71-year-old woman after two months of using Imunex

ANNEX 4.2.6. General health indicators, including Activation rate after two months of use of Imunex

PROPOSAL FOR INVENTION NAME: Imunex Method and Compositions

ABSTRACT: The proposed method and compositions for Imunex aims to improve the prophylaxis and healing of the immune system, to recharge the body with energy, as well as to fight for longevity. Imunex is available as a Biologically Active Food Supplement (BAD). The main substance is the powder from the fluid that is released by dairy animals such as cows, buffaloes and others - 48 hours in preparation for parturition (different period for different animals) and 72 hours after the birth of the new animal.

With the use of Imunex, the body is naturally nourished by immunoglobulins, ready-made antibodies that activate Vlimfacies, the humoral immune response and specific immune defenses. Containing lactoferrin, a proline-rich polypeptide (PRP), Interleukins, χ-Interferon and others in turn regulate the thymus gland, activate T lymphocytes and Th-Ι cells and stimulate cellular immune response and cellular immune defense. Self-regulation in the body is aided by the growth factors, growth hormone, oxytocin, prolactin, etc. in the preparation, which help young organisms and at the same time regenerate muscles, tissues, bones, nerve cells in adults and open up endless possibilities for prolongation life expectancy.

The amino acids contained are an essential protein food and, together with alkaline earth metals, complement the effect of the proposed immune modulator of animal origin.

Supplements can be used by adults and children.

TECHNICAL FIELD

The invention relates to a method and compositions that enhance human immunity is to use unconventional approaches and improve relationships with the universe and healthy lifestyles. A new immune modulator will take its place in the prevention and improvement of the immune system, supplying the body with passive antibodies (immunoglobulins) and assisting in the treatment of immune diseases, mainly related to specific immune modulation and humoral immune responses. A number of constituents of the preparation (lactose * »·· • Σ · · ·. · · <

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ο rin, a proline-rich polypeptide (PRP, cytokines) stabilize the thymus and activate T-leukocytes and Th-Ι cells, thereby stimulating cellular immune response and cellular immune defense. This is also a breakthrough in the fight against cancer and related AIDS diseases. Growth factors and growth hormone, oxytocin, prolactin and others, which are contained in the preparation are of particular importance for enhancing the immunity of young organisms and at the same time regenerate tissues, muscles, bones, nerves, skin in the elderly and offer endless possibilities for prolongation of life expectancy. BACKGROUND OF THE INVENTION In the world and already in the Bulgarian practice, there are a number of preparations available on the market as Biologically Active Supplements (BAD) for food - recommended for improving the immune system such as echinacea, cordyceps, cat nail, spirulina and others, mainly plant base. The disadvantage of these products, also experimented by the authors, is that they have to be applied for a long time and repeated application over a period of time. This led the authors to turn to other compositions of animal origin and to look for mechanisms closest to those in the human body and to activate the processes of self-regulation, as is the case with the proposed immune modulator Imunex. TECHNICAL NATURE The essence of the invention is to find a method and our formula of substances that allow the application of a new immune modulator of animal origin. Over time, the human body loses its natural immune factors and becomes less resistant to disease. Microorganisms and antigens in the form of various infections such as bacteria, viruses, parasites, fungi and amoeba attack the body of 4OBeKa (Streptococcus, Staphylococcus, Salmonella, E-coli, Candida, etc.) The ability of the human body to renew tissues, to restore

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Elasto-loss is reduced and energy lost becomes counteracted, skin ethics and muscle mobility are left behind and the body needs to be assisted in activating self-regulatory processes, enhancing protective forces and extending life expectancy.

So, for several years now, the team has been struggling to resolve the complex processes of creating and experimenting a method of immunotherapy compositions related to improving immunity.

The method of creating Imunex went through many difficulties.

It is possible to collect a thick yellowish substance (48 hours in preparation for birth - a period different for different animals and 72 hours after birth) in cows, buffaloes, goats, sheep, mares and donkeys.

The collection of primary material requires extremely high hygiene and better results are obtained with the use of milking machines than with manual milking. It is preferable to feed the animals naturally, free of grazing. (Annex 1a)

The process of drying the primary material should be near the optimum, which was related to the effect of a low degree of heat below 45 degrees celsius. The liquid of the substance is evaporated in vacuo. The low temperature does not allow the proteins to be denatured. The humidity should not be lower than 5% and higher than 11%.

Such technology aims to preserve the natural immune factors of the product and in particular the immunoglobulins.

The collection of this yellowish substance, which is excreted by the mammary glands 48 hours before birth (different period for different animals) and during the first 72 hours after birth, must be strictly controlled, as the quality of the future product will be dramatically impaired after this period. . As a rule, about 63 liters of fluid are released into cows within 72 hours. The highest immunoglobulin content is obtained within two days of birth.

Often, animals are given antibiotics, disinfectants and others from which primary material should be cleaned.

When making high demands on the material, it must be degreased (as in the method, after which the product turns yellowish pale yellowish). It is desirable to remove · »» ·

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not lactose.

In the preparation of the mixtures, they are used for lubricants - trypsin and protease, which prevent the destruction of acids in the stomach. Yogurt with its probiotics also plays a positive role in this regard.

The main component of the present compositions is a natural, thick yellowish substance that the mammary glands secrete. When properly prepared, the powder contains 25-30% of immunoglobulins in 200mg of said substance. IG In fact, these are ready-made antibodies that exert specific immunomodulation. (Annexes 1a, 16, 1c and 2)

It also contains - growth factors (epithelial tissue growth factors - IGF associated with wound healing, ulcers and mucosal strengthening of all systems; insulin-like factor - IGF-1 associated with regulation of blood sugar and cholesterol; transforming growth factors- TGF A and B, related to cartilage and bone formation) and growth hormone (h.GH), which stimulates the pituitary gland, regulates growth in young organisms and improves immunity. Growth factors and growth hormone regenerate muscles, tendons, bones, nerve cells, tissues, skin, thus regenerating adult organisms; transmit information to cells along with the hormones of the endocrine glands, reaching this level of DNA and RNA recovery; rejuvenation reserves and longer life expectancy and longevity are opened. The content of prolactin, a breast-feeding, oxytocin-stimulating, delivery component, is of particular importance to young organisms. (Annex 3)

Lactoferrin and cytokines activate T-lymphocytes and Th-1 cells associated with the fight against cancer and related AIDS diseases; Proline-rich polypeptide (PRP) - regulates thymus function. The last three components stimulate the cellular immune response and cellular immune defenses, including the cells for tolerance to stop the immune defenses. (Annex 3)

Vitamins (especially from group B, A, D), alkaline earth metals (Ca, K, Se, Ζη, Ρ, S), enzymes, amino acids (proline, leucine, valine, lysine, alanine, methionine, etc.) , oligosaccharides (protect gastric walls from harmful germs), lactalbumin (stimulates serotonin secretion).

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and others are of particular importance in metabolism, improvement of the gastrointestinal tract, are involved in the fight against multiple sclerosis, chronic fatigue syndrome, scleroderma, arthritis and osteoporosis. Insulin-like growth factor (IGF-1) - regulates blood sugar and cholesterol improves metabolism and function of the gastrointestinal tract; recovers muscle and DNA and RNA.

(See Annexes 1a, 2 and 3)

Studies show that the powder dried from the aforementioned liquid has the ability to:

- kill bacteria and viruses;

- stimulate the tissues to renew;

- Enrich the immune system with antibodies against attackers;

- increase the body's resistance forces;

- improve the intestinal flora and gastrointestinal tract;

-to stimulate the pituitary gland in the release of growth hormone, prolactin, oxytocin, etc .;

- stimulate through the pituitary gland - hormones of the thyroid gland, of the adrenal glands, of the pancreas, of the reproductive glands; stimulate neuropeptides in the pituitary-hypotomous region;

- through growth factors to support the tissues and all systems in the body by opening a new page to prolong life and longevity;

- to support the thymus gland to activate Th-Ι cells and stimulate the cellular immune response, as well as to fight cancer and related AIDS diseases;

- a powerful antioxidant, healing wounds, fighting tumor and cancer cells; rescuer from chronic fatigue syndrome, multiple sclerosis, scleroderma, etc.

- improves digestion of food from the intestines, increases metabolism, strengthens muscles, increases levels of glucose in the blood and brain.

The results of application of the drug include a sharp increase in energy in the body. (Appendices 4.1.a, 4.16, 4.2a and 4.26 with an author experiment - where it is seen that the activation factor, as the main energy characteristic after two months of Imunex application, has increased from 0.92 to 3.31 and is in the norm of 2.0 -4.0).

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The mechanism of action of the immune modulator - Imuneks on the human body rests on the blood flow that enters the blood depots, among which are the lymph nodes and the spleen. These depots accumulate B lymphocytes, which form antibodies and exert specific immunomodulation and a humoral immune response in the body. (See Annex 2)

Maternity mammary glands contain all classes of immunoregulators. The immunoglobulin Ig.M - contains those antibodies that are the first to respond to the infection; Ig.G are antibodies that make up 70% of all antibodies and act through the bloodstream, plasma, and tissues. (They are mainly the subject of our attention). Annexes 1a, 1c and 2);

Ig.A- these are the antibodies that appear on the surface of the lungs and intestines and with their secretions affect the bronchi and intestines; (Annexes 1a and 16). Ig.E are antibodies that are associated with allergic reactions; Ig.D are antibodies that are found on the surface of B cells.

The use of the immune modulator is related to: 1. the effect of blood flow; 2. with a sharp improvement of the gastrointestinal tract and intestinal flora; 3. with renewal and repair of tissues and muscles, bones, nerves; a sharp increase in energy; improving the body's resistance and resilience; 5. the key to longevity; 6. improving the specific immunomodulation and humoral immune response in the body; 7. activation of the thymus, T-lymphocytes and Th-1 cells, which complement the range of natural immunomodulators of the fight against free radicals, diabetes, cancer and similar diseases of AIDS through activation of the cellular immune response in the body and cellular immune protection.

The growth hormone content and a number of growth factors, prolactin, oxytocin and more. is associated with the pituitary-hypothalamic area, which regulates the effect on hormones and neuropeptides throughout the body; young organisms are stimulated and the energy, vitality and performance of adults are increased. Growth hormone and growth factors affect the thyroid gland, pancreas, adrenal glands, reproductive glands and thus the immune system components affect the whole body. (Annex 3).

EXAMPLES FOR THE IMPLEMENTATION OF THE INVENTION

The invention is a Biologically Active Animal Supplement of animal origin - a substance produced by the mammary glands of the mammary gland - 48 hours in preparation for birth and 72 hours after birth.

Example 1 - A pale yellowish powder from the substance of dairy animals (up to 72 hours after birth), enriched with yogurt, which is the natural formula for supplying the body with antibodies, which increases the body's resistance, dramatically increasing the body's energy and vitality also improving digestion and metabolism, having a positive effect on the intestines, tissues, muscles, bones, nerves and skin.

The ratio of basic substance to yogurt is in three variants: 97% Immunex and 3% yogurt; 95% - Imunex and 5% - yogurt; 90% - Imunex and 10% yogurt.

Contents: immunoglobulins; growth factors, oxytocin, prolactin; amino acids (proline, trypsin, leucine, valine, lysine, alanine, etc., which are a wonderful food for the body and supply it with the eight essential amino acids); bifidobacterium, lactobacillus acidophilus and other probiotics help the intestinal flora and all processes in the body; alkaline earth metals (Ca, K, Se, Fe, Zn, P and compounds, S and compounds), etc. complete the wide range of benefits for the body.

Example 2 - A pale yellowish powder from the liquid of dairy animals (up to 72 hours after birth) with fruit mixtures - peach, cherry, orange and others. (The content of fruit mixes is 1% - mainly to improve taste)

The results are the same with Example 1 as this substance is for children.

Contents: immunoglobulins, prolactin, oxytocin, growth factors, polypeptide rich in proline, lactoferrin, cytokines, oligosaccharides, amino acids, alkaline earth metals (Ca, K, Se, Fe, P- and compounds, S and compounds and etc.) and others.

EXAMPLE 3 Pale yellowish milk powder from dairy animals (up to 72 hours postpartum) mixed with Collagen H-bone powder of young (not more than 1.5 years) cattle - Imunex-15% base substance and Collagen H - 85%.

The results are the same as Example 1 plus the effect of collagen for improvements in arthritis, osteoporosis and others.

The combination of Imunex with Collagen H produces a powerful anti- * Ό oxidant that allows for lasting results in and out of production, enhancing durability and performance and creating stability and comfort for the body.

Contents: immunoglobulins; oxytocin, prolactin, growth factors; amino acids (glutamic acid, proline, alanine, arginine, leucine, lysine, glycine and others); alkaline earth metals (Ca, Mg, Ka, Na, Fe, P2O5, S and sulfur compounds and others).

EXAMPLE 4 Pale yellowish powder from the liquid of dairy animals (up to 72 hours after birth) enriched with alkaline-earth metals in a volume 3-4 times greater than that contained in the natural alkaline-earth substance in the parent substance.

The results are similar to the previous signs plus the effect of enrichment with alkaline earth metals such as Ca, K, Se, Zn, Cr and others, especially in arthritis, osteoporosis, bone healing, muscle stabilization, nerve cell and tissue repair.

The preparations are fasted, with a single teaspoon - 3 times a day for adults;

In babies (up to 1 year old), a teaspoonful per day is given; for children from 1-5 years old - a teaspoon daily; In children 6-12 years of age, a teaspoon is given twice a day;

The powder can be taken with yogurt, and the fruit with milk is 9.

dissolve in water or juice. For therapeutic purposes, dosages may be doubled in the elderly. The preparation is harmless.

Example 5 - Powder from the milk of dairy animals (up to 72 hours after birth) can be added to creams and masks, achieving good cosmetic effect in nourishing the skin, fighting wrinkles and rejuvenating. Such preparations require a longer experimentation time for better results. When handling the powder of Example 5, the substance is not degreased as the fats serve to coat the ingredients.

When the powder of the specified fluid is mixed with distilled water or with saline to form a slurry, it can be used for rapid healing of wounds, postoperative sutures and burns.

Claims (1)

Patent Claims / The first (independent) claim is a method and compositions for Imunex as a Biologically Active Supplement / dietary supplements) for food of animal origin, starting from raw / yellowish dense substance, which is excreted from the mammary glands 48 hours in preparation for birth (period different in different animals) and 72 hours after the birth of the new animal / dust shows remarkable qualities with 25-30% of immunoglobulins (in 200 mg IG) that feed the body with antibodies mainly through blood flow, tissues and intestinal flora; high content of amino acids, including eight essential (Annexes 1a, 2); alkaline earth metals such as potassium, calcium, selenium, phosphorus, sulfur, zinc and others; growth factors, growth hormone (hGH), oxytocin, prolactin, etc .; insulin-like growth factor (IGF-1); oligosaccharides and glycoproteins; lactoferrin, poly proline (PRP) h cytokines; probiotics, etc .; visima) claim related to the method and compositions of Imuneks with the fact that the drying of the powder by the method is carried out πρι / possible low temperatures (below 45 degrees celsius) and the liquid / is evacuated in order to preserve the valuable qualities and properties of the starting material and avoid protein denaturation; the humidity should not be less than 5% and higher than 11%. Third (and dependent) claim related to peptide rich method and compositions Second (of; characterized by ANNEX la. ANALYTICAL PROTOCOL OF IMMUNEX RESEARCH FOR IMMUNOGLOBULINS, PROTEINS AND MICROBIAL NUMBER (BULBIO Ltd. Laboratory Study - Sofia) • 9 BUL BIO - NCSPB EOOD BB - NCIPD Ltd. 1504 Sofia, 26 Yanko Sakazov Blvd. Fax: ** 359 2 943 30 75 Phone Manager: ** 359 2 446 191 e-mail: nenkov@bulbio.com 1504 Sofia, 26 Yanko Sakazov blvd. Fax:: ** 359 2 943 30 75 Telephone General Manager: ** 359 2 446 191 E-mail: nenkov @ bulbio.com ANALYTICAL PROTOCOL IMUNEX Test The result Appearance - Visually: - before reconstitution - after reconstitution pale yellow powder pale yellow suspension Total nitrogen content (mg%) / Kjeldahl determination 5.80 mg per 100 mg Protein nitrogen content (mg%) / Kjeldahl determination 5.23 mg per 100 mg Microbial contamination - total number of micro-organisms (CFU / d) - mushrooms (CFU / d) - E. coli, Salmonella, P.aeruginosa, S. aureus 300 CFU I g OCFU / g is missing Immunoglobulins: (double Manchini immunodiffusion) - IgG - IgM - IgA discover open discover open