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BG49719A3 - Метод за получаване на варианти на хирудина и приложението им - Google Patents

Метод за получаване на варианти на хирудина и приложението им

Info

Publication number
BG49719A3
BG49719A3 BG082035A BG8203587A BG49719A3 BG 49719 A3 BG49719 A3 BG 49719A3 BG 082035 A BG082035 A BG 082035A BG 8203587 A BG8203587 A BG 8203587A BG 49719 A3 BG49719 A3 BG 49719A3
Authority
BG
Bulgaria
Prior art keywords
application
hirudin variants
obtaining
yeast
obtaining hirudin
Prior art date
Application number
BG082035A
Other languages
English (en)
Inventor
Michael Courtney
Eric Degryse
Gerard Loison
Yves Lemoine
Original Assignee
Transgene S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgene S.A. filed Critical Transgene S.A.
Publication of BG49719A3 publication Critical patent/BG49719A3/bg

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Control Of Multiple Motors (AREA)

Abstract

Методът намира приложение за получаване на антикоагуланти с висока степен на инхибиторна активност за нуждите на терапевтиката. Той се осъществява чрез трансформация на дрожди с помощта на специално конструиран векторен плазмид, култивирането на тези дрожди и отделяне на получения в културалната среда хирудин.5 претенции, 2 фигури
BG082035A 1986-12-01 1987-12-01 Метод за получаване на варианти на хирудина и приложението им BG49719A3 (bg)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR868616723A FR2607517B2 (fr) 1986-12-01 1986-12-01 Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu

Publications (1)

Publication Number Publication Date
BG49719A3 true BG49719A3 (bg) 1992-01-15

Family

ID=9341393

Family Applications (1)

Application Number Title Priority Date Filing Date
BG082035A BG49719A3 (bg) 1986-12-01 1987-12-01 Метод за получаване на варианти на хирудина и приложението им

Country Status (24)

Country Link
US (1) US5650301A (bg)
EP (1) EP0273800B1 (bg)
JP (1) JP2567263B2 (bg)
KR (1) KR960011918B1 (bg)
AT (1) ATE73462T1 (bg)
AU (1) AU617989B2 (bg)
BG (1) BG49719A3 (bg)
CA (1) CA1341416C (bg)
CS (1) CS276245B6 (bg)
DE (1) DE3777367D1 (bg)
DK (1) DK173247B1 (bg)
ES (1) ES2032465T3 (bg)
FI (1) FI99211C (bg)
FR (1) FR2607517B2 (bg)
GR (1) GR3004556T3 (bg)
HU (1) HU213871B (bg)
IE (1) IE60544B1 (bg)
MC (1) MC1884A1 (bg)
NO (1) NO177500C (bg)
PL (1) PL157254B1 (bg)
PT (1) PT86233B (bg)
RO (1) RO106890B1 (bg)
SU (1) SU1687032A3 (bg)
ZA (1) ZA879011B (bg)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2628429B1 (fr) * 1988-03-08 1990-12-28 Transgene Sa Variants de l'hirudine, leurs utilisations et les procedes pour les obtenir
GB8817161D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Modified proteins
GB8817160D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Novel proteins
US5284768A (en) * 1988-08-24 1994-02-08 Sanofi Signal peptide, DNA sequences coding for the latter, expression vectors carrying one of these sequences, gram-negative bacteria transformed by these vectors, and process for the periplasmic production of a polypeptide
FR2645175B1 (fr) * 1989-03-31 1994-02-18 Transgene Sa Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche
FR2645174B1 (fr) * 1989-03-31 1994-01-07 Transgene Sa Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant
US5879926A (en) * 1989-03-31 1999-03-09 Transgene S.A. Yeast strains for the production of mature heterologous proteins, especially hirudin
FR2646437B1 (fr) * 1989-04-28 1991-08-30 Transgene Sa Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
FR2656531B1 (fr) * 1989-12-29 1992-04-24 Sanofi Sa Promoteur artificiel pour l'expression de proteines dans le levure.
US5118790A (en) * 1990-07-24 1992-06-02 Sri International Analogs of hirudin
WO1992008736A1 (en) * 1990-11-08 1992-05-29 Nippon Mining Company Limited Hirudine mutant, production thereof, anticoagulant, secretory vector, microorganism transformed by said vector, and production of product from said microorganism
US5837808A (en) * 1991-08-20 1998-11-17 Baxter International Inc. Analogs of hirudin
US5407822A (en) * 1991-10-02 1995-04-18 Sanofi Artificial promoter for the expression of proteins in yeast
US20210317162A1 (en) * 2019-04-16 2021-10-14 Sungen Bioscience Co., Ltd. Method for extraction and purification of hirudin mutant and use thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341417C (fr) * 1984-03-27 2003-01-21 Paul Tolstoshev Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
EP0168342B1 (de) * 1984-06-14 1991-07-03 Ciba-Geigy Ag Verfahren zur Herstellung von Thrombin-Inhibitoren
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE3445517C2 (de) * 1984-12-13 1993-11-18 Ciba Geigy Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
DE3689525D1 (de) * 1985-07-17 1994-02-24 Hoechst Ag Neue Polypeptide mit blutgerinnungshemmender Wirkung, Verfahren zu deren Herstellung bzw. Gewinnung, deren Verwendung und diese enthaltende Mittel.

Also Published As

Publication number Publication date
DK173247B1 (da) 2000-05-22
RO106890B1 (ro) 1993-07-30
AU8188087A (en) 1988-06-02
NO177500C (no) 1995-09-27
ATE73462T1 (de) 1992-03-15
KR880007729A (ko) 1988-08-29
JP2567263B2 (ja) 1996-12-25
MC1884A1 (fr) 1989-01-24
EP0273800A3 (en) 1988-07-20
DK627687A (da) 1988-06-02
GR3004556T3 (bg) 1993-04-28
CS8708732A2 (en) 1991-07-16
CA1341416C (fr) 2003-01-21
PL269164A1 (en) 1988-08-18
US5650301A (en) 1997-07-22
FI875295A0 (fi) 1987-12-01
IE60544B1 (en) 1994-07-27
PT86233A (fr) 1987-12-01
EP0273800B1 (fr) 1992-03-11
ZA879011B (en) 1988-05-27
NO874905L (no) 1988-06-02
FI99211B (fi) 1997-07-15
CS276245B6 (en) 1992-05-13
KR960011918B1 (ko) 1996-09-04
FI99211C (fi) 1997-10-27
EP0273800A2 (fr) 1988-07-06
FR2607517B2 (fr) 1989-12-22
DE3777367D1 (de) 1992-04-16
IE873239L (en) 1988-06-01
HUT45564A (en) 1988-07-28
PT86233B (pt) 1990-11-07
NO177500B (no) 1995-06-19
PL157254B1 (pl) 1992-05-29
FR2607517A2 (fr) 1988-06-03
NO874905D0 (no) 1987-11-25
AU617989B2 (en) 1991-12-12
FI875295L (fi) 1988-06-02
DK627687D0 (da) 1987-11-30
HU213871B (en) 1997-11-28
JPS63152987A (ja) 1988-06-25
ES2032465T3 (es) 1993-02-16
SU1687032A3 (ru) 1991-10-23

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