AU649066B2 - Circular extension for generating multiple nucleic acid complements - Google Patents

Description

U FI ]HATE I/U0/92 AOJP DATE 26/03/92 APPLN. I) 84173 91 PCT NUMBFR PCT/IlS91/05067 INTERN ATi .'REATY (PCT) (51) International Patent Classification 5 (11) International Publication Number: WO 92/01813 C12Q 1/68, C12P 19/34 Al (43) International Publication Date: 6 February 1992 (06.02.92) (21) International Application Number: PCT/US91/05067 (81) Designated States: AT (European patent), AU, BE (European patent), CA, CH (European patent), DE (Euro- (22) International Filing Date: 17 July 1991 (17.07.91) pean patent), DK (European patent), ES (European patent), FR (European patent), GB (European patent), GR (European patent), IT (European patent), JP, KR, LU Priority data: (European patent), NL (European patent), NO, SE (Eu- 558,645 25 July 1990 (25.07.90) US ropean patent).

(71) Applicant: SYNGENE, INC. [US/US]; 10030 Barnes Can- Published yon Road, San Diego, CA 92121 With international search report.

Before the expiration of the f-,ne limit for amending the (72) Inventors: RUTH, Jerry, L, 10267 Avenida Magnifica, claims and to be republished in the event of the receipt of San Diego, CA 92131 DRIVER, David, A. 5142 amendments.

Biltmore Street, San Diego, CA 92117 (US).

(74) Agents: CAMPBELL, Cathiyi et al.; Pretty, Schroeder, y Brueggeman rClark. 444 South Flower Street, Suite 2000, Los Angeles, CA 90071 (54)Title: CIRCULAR EXTENSION FOR GENERATING MULTIPLE NUCLEIC ACID COMPLEMENTS (57) Abstract 0 A process for generating multiple linear complements of a single strand, circular nucleic acid template containing at least one cleavage site is described. The process consists of combining the single strand, circular nucleic acid template with polynucleo- H tide primers under conditions sufficient for hybridization; extend- 0 ing the polynucleotide primer more than once around the circle to generate a complementary displacement of more than one contiguous complement of the single strand, circular nucleic acid template. Also described is a process of synthesizing novel single strand, circular nucleic acids between 30 and 2200 nucleotides. 4 The process consists of synthesizing a linear polynucleotide; combining the linear polynucleotide with a complementary linking oligonucleotide under conditions sufficient for hybridization; and ligating the linear polynucleotide to produce a single strand, circular nucleic acid. 3 K K" WO 92/01813 PCT/US91/05067 CIRCULAR EXTENSION FOR GENERATING MULTIPLE NUCLEIC ACID COMPLEMENTS BACKGROUND OF THE INVENTION This invention relates generally to the generation of multiple complementary copies of a nucleic acid and, more particularly, to the generation of such complementary copies using novel circular templates.

Recombinant DNA techniques have revolutionized molecular biology and genetics by permitting the isolation and characterization of specific DNA fragments. Of major impact has been the exponential amplification of small amounts of DNA by a technique known as the polymerase chain reaction (PCR). The sensitivity, speed and versatility of PCR makes this technique amenable to a wide variety of applications such as medical diagnostics, human genetics, forensic science and other disciplines of the biological sciences.

PCR is based on the enzymatic amplification of a DNA sequence that is flanked by two oligonucleotide primers which hybridize to opposite strands of the target sequence.

The primers are oriented with their 3' ends pointing towards each other. Repeated cycles of heat denaturation of the template, annealing of the primers to their complementary sequences and extension of the annealed primers with a DNA polymerase result in the amplification of the segment defined by the 5' ends of the PCR primers.

Since the extension product of each primer can serve as a template for the other primer, each cycle results in the exponential accumulation of the specific target fragment, up to several millionfold in a few hours. The method can be used with a complex template such as genomic DNA and can amplify a single-copy gene contained therein. It is also capable of amplifying a single molecule of target DNA in a WO 92/01813 PCT/US91/05067 2 complex mixture of RNAs or DNAs and can, under some conditions, produce fragments up to ten kbp long. The PCR technology is the subject matter of United States Patent Nos. 4,683,195, 4,800,159, 4,754,065, and 4,683,202 all of which are incorporated herein by reference.

While the possible applications of PCR are numerous, they are limited to those situations where the DNA sequence is known with sufficient specificity to design two primers which hybridize to opposite strands of the target sequence.

Moreover, the repetitive cycles of heating and cooling, primer hybridization and polymerization are time consuming and optimally performed on expensive, automated thermocycling devices.

Since the advent of PCR, several additional methods have been devised to amplify the copy number of a target sequence. Such methods employ a variety of strategies to achieve the amplification of a small amount of target sequence; however, all have inherent drawbacks.

The ligation chain reaction (LCR) is one such method.

See PCT publication WO 89/12696, which is incorporated herein by reference. LCR uses a ligase enzyme to join preformed stretches of DNA, termed "probes." Once these probes find their complementary target sequences and anneal to them, the ligase joins the target sequences together, setting up a situation analogous to that found in PCR: two templates are formed, both of which can serve in the next cycle to generate more copies of the target sequence.

QB replicase is another alternative for exponentially amplifying a target sequence. This method relies on the ability of the enzyme QB replicase to generate copies of recombinant RNA molecules. QB replicase is an RNA-directed tRNA polymerase of bacteriophage QB. The sequence to be amplified is cloned into a gene coding for a natural PC/US91/05067 WO 92/01813 3 template of QB, such as MDV-1 RNA, and produced as a recombinant RNA. For target amplification, the recombinant RNAs hybridize to complementary DNA and serve as templates for RNA synthesis. See Kramer and Lazardi, Nature 339:401- 402 (1989), which is incorporated herein by reference.

Transcription-based amplification (TAS) systems have also been developed to amplify the copy number of a target sequence, as described in PCT publication WO 88/10315. The cycle begins with the synthesis of cDNA from a target RNA sequence. TAS employs specialized 5' primers which contain a RNA polymerase promoter sequence. The use of these primers results in a promoter sequence being incorporated into the double-stranded product. Transcription from the promoter results in the production of multiple RNA copies.

These copies can then be reprimed for additional rounds of cDNA synthesis and transcription. A disadvantage to this method is that it requires accurate execution of at least three enzymatic steps. Therefore, full length products may be difficult to obtain.

Finally, enhancement of target sequences by priming, extension, restriction and reannealing of a linear fragment containing multiple copies of the target sequence has been described. See Becker et al., EPA No. 0 300 796, which is incorporated herein by reference. Although this method generates additional copies of target sequence with each cycle, it has a major disadvantage in that the template, which consists of multiple copies of the target sequence, is destroyed by restriction at the end of each cycle and cannot be reused. Moreover, multiple copies must be present in each template to provide any increase in copies, and product transcripts are of highly variable length.

Most of the methods described above rely on thermal cyclization for denaturating and reannealing of products and templates. Methods such as the QB replicase technique WO 92/01813 PCT/US91/05067 4 additionally require specialized recombinant RNA templates.

Moreover, all methods employ linear templates, some of which are specially constructed to contain repetitive copies of the sequence to be amplified.

There thus exists a need for a method to generate multiple copies of a target sequence without the use of degenerative or specialized linear templates and without time consuming thermal cyclizations. Such a method would be of critical importance to increasing the efficiency of medical diagnosis. The present invention satisfies this need through the use of novel circular templates and provides related advantages as well.

SUMMARY OF THE INVENTION The invention provides a process for generating multiple linear complements of a single strand, circular nucleic acid template containing at least one cleavage site. The process includes combining the single strand, circular nucleic acid template with polynucleotide primers under conditions sufficient for hybridization; and extending the polynucleotide primer more than once around the circle to generate a complementary displacement of more than one contiguous complement of the single strand, circular nucleic acid template. The invention further provides a process of synthesizing novel single strand, circular nucleic acids between 30 and 2200 nucleotides, by synthesizing a linear polynucleotide and ligating the linear polynucleotide to produce a single strand, circular nucleic acid.

WO 92/01813 PCT/US91/05067 BRIEF DESCRIPTION OF THE FIGURES FIGURE 1 is a schematic diagram of a single-stranded circle. A represents a target sequence, 10-1000 bases in length, complementary to the polynucleotide primer; B and C represent sequences, 0-400 bases in length, which are used as detection sequences for circle complement; d-f represent linking sequences of 0-100 bases in length.

Any of A-e may contain one or more restriction sites.

FIGURE 2 is a schematic diagram for generating multiple nucleic acid complements. G represents singlestranded circle primed for extension by a target polynucleotide; H represents excess single-stranded circle; I represents G extended by polymerizing enzymes after displacement to form multiple linear concatemers; J represents I cleaved into smaller fragments; K represents H primed by fragments from J; Reaction 1 is extension of primer by polymerizing enzymes; Reaction 2 is cleavage of I to form J; Reaction 3 is reannealing of fragments from J to circles H.

FIGURE 3 is a schematic diagram of constructs for making single-stranded circles. L represents one linear fragment circularized by linker m before ligation; N represents formation of a circle composed of two or more fragments o and p, each 15-1100 bases in length which may be similar or different in sequence; Q represents formation of a circle capable of forming at least one stem structure by ligation of two subunits containing loops r and s; T Lepresents formation of a circle capable of forming at least one stem structure by ligation of a single linear fragment containing loops r and s; U represents formation of a circle capable of forming one or more stem structures with ends which can be ligated; V represents construction of a circle by restricting a stem structure at site w, allowing formation and ligation of the product circle.

PCT/US91/05067 WO 92/01813 6 DETAILED DESCRIPTION OF THE INVENTION This invention is directed to a simple, inexpensive and rapid process for generating multiple linear complements from a single strand, circular nucleic acid and to a process for making such novel circular nucleic acids.

An important advantage of generating linear complements from single strand, circular nucleic acids is that the need for differential thermal conditions at each step in the process is alleviated. All steps in the process can be performed isothermally which also alleviates the need for expensive thermocycling devices. Thus, the process is applicable to use for detecting nucleic acids in such areas as medical diagnosis and infectious diseases.

In one embodiment, the process for generating multiple linear complements from single strand, circular nucleic acid templates involves combining the template with polynucleotide primers, such as an analyte, in a biological sample. The combining step is performed under conditions sufficient for hybridization so that a primer-template is formed between the primer and a complementary region within the circular template and can be used for polymerase extension. The analyte can be, for example, of viral or bacterial origin and may be DNA or RNA.

Extending the primer-template with a polymerase such as Klenow fragment generates a linear concatemer of the circular template through strand displacement synthesis.

The synthesized DNA segment is thereby replaced with a new strand each time the polymerase passes around the circular template to produce a complementary displacement product.

The circular template can contain a cleavage site such as a restriction endonuclease cleavage site. The restriction endonuclease site is incorporated into the complementary displacement product and can be used, when PC/US91/05067 WO 92/01813 7 hybridized to a restriction polynucleotide, to cleave the displacement product into unit length linear complements.

The restriction polynucleotides are preferably the single strand, circular nucleic acid templates, wherein the restriction site on the circular template has been modified so as to be refractory to endonuclease cleavage.

Modification can be accomplished by methylation, for example.

The linear complements generated by strand displacement synthesis and cleavage can be further used to prime additional circular templates for production of additional linear complements. All components necessary for polymerase extension and cleavage of the displacement product to linear complements remain available for use in additional cycles. In this way, a large number of linear complements can be generated from a very small number of initial polynucleotide primers.

If the polynucleotide primers are, for example, analytes in a biological sample, then the production of linear complements signifies the presence of the analyte.

Detection of linear complements or displacement products can be performed by methods known to one skilled in the art. The invention, therefore, may be used for rapid and efficient detection of infectious diseases and diagnoses of genetic disorders.

In another embodiment, the process for making novel circular nucleic acids for use in the process for generating linear complements involves the chemical synthesis of linear polynucleotides and their combination with linking oligonucleotides. The linking oligonucleotides have sequences complementary to the ends of the linear polynucleotides and can be hybridized to the linear polynucleotides. Hybridization of the two types of molecules will bring the 5' and 3' ends of one or more PCT/US91/05067 WO 92/01813 8 linear polynucleotide together. Ligating the ends of the linear polynucleotides together produces a single strand, circular nucleic acid.

As used herein, the term "single strand, circular nucleic acid template" or "circular template" refers to a single strand nucleic acid polymer which has been joined through its 5' and 3' ends to produce a circular molecule.

These single strand, circular templates include molecules produced by methods disclosed herein for synthesis of small single-stranded circles (between 30 and 750 nucleotides) as well as molecules produced in vivo or in vitro through bacteriophage replication mechanisms. Circular molecules produced entirely through chemical synthesis are included as well. The circular templates can be either deoxyribonucleic or ribonucleic acids.

As used herein, the term "cleavage site" refers to a nucleotide sequence in which the phosphodiester backbone is selectively broken. For example, a nucleotide sequence recognized by a restriction endonuclease is a cleavage site because the enzyme will cut the phosphodiester backbone at selective sites within the sequence. Such cleavage sites may be single or double-stranded, depending on the endonuclease. Also included are chemical cleavage sites such as pyrimidine and purine cleavage reactions performed in Maxam and Gilbert sequencing, or cleavage through chemical methods such as oxidation as described in United States Patent No. 4,795,700, which is incorporated herein by reference.

As used herein, the term "polynucleotide primer" refers to any nucleic acid with a complementary sequence sufficient to hybridize to the single strand, circular template and be used as a substrate for polymerase extension reactions. This term includes an analyte within a mixture of nucleic acids, synthesized oligonucleotides PCT/US91/05067 WO 92/01813 9 and linear complements produced from displacement products.

RNA as well as DNA primers can be used. The length of the primer can vary so long as the 3' terminal nucleotides form sufficient base pairs to effectively prime the template.

Therefore, the term "primer-template" as used herein refers to a primer which is hybridized to a single strand, circular template and can be used in polymerase extension reactions.

As used herein, the term "displacement synthesis" refers to polymerase extension reactions in which one strand of a double-stranded region is displaced by the processivity of the enzyme. Effectively, the strand is peeled away from the template simultaneously with new strand synthesis. Therefore, the term "complementary displacement product" or "displacement product" as used herein refers to the displaced complementary strand which is peeled away from the template as the polymerase proceeds around the circular template.

As used herein, the term "restriction polynucleotide" refers to a nucleic acid, preferably a single-stranded deoxyribonucleic acid, which encodes a restriction endonuclease site so that when hybridized to a complementary strand it forms a functional restriction endonuclease site. The length of the restriction polynucleotides can vary so long as, when in duplex form, the appropriate restriction enzyme will recognize the sequence and cleave the phosphodiester bonds. Restriction polynucleotides may include linear poly- and oligonucleotides as well as circular nucleic acids such as the circular templates described herein.

As used herein, the term "linear complement" refers to the complementary sequence of the single strand, circular template. A linear complement is the extension product from a primer-template substrate and includes concatemers WO 92/01813 PCT/US91/05067 produced by displacement synthesis as well as individual copies of the circle template.

As used herein, the term "blocked" refers to modifications of an oligo- or polynucleotide which limits the function of the modified group. Such functions may include polymerization of a nucleotide or nucleotides.

Blocking groups can include, for example, attachment of chemical moieties, organic polymers and macromolecules to the ligo- or polynucleotide. Also included is the reverse addition of a nucleotide to the oligo- or polynucleotide.

Modifications encompass additions to the nucleotide bases, the sugar moieties and the phosphate backbone.

The invention provides a process for generating multiple linear complements of a single strand, circular nucleic acid template containing at least one cleavage site by combining the single strand, circular nucleic acid template with polynucleotide primers under conditions sufficient for hybridization; extending the polynucleotide primer more than once around the circle to generate a complementary displacement product comprising more than one contiguous complement of the single-stranded, circular nucleic acid template; and cleaving the displacement product to generate linear complements of the circle template.

As shown in Figure single strand, circular templates are first hybridized to polynucleotide primers.

The resultant primer-templates are substrates for polymerase directed synthesis of nucleic acids. For example, if the template is DNA, then any of the known DNA polymerases such as E. coli DNA polymerase I, Klenow fragment, T4 or T7 DNA polymerases, modified polymerases, reverse transcriptase, Tag polymerase, Bst polymerase, and T. flavins polymerase can be used to synthesize a complementary DNA strand. Alternatively, if the template PCI'7 US9 1/05067 WO 92/01813S/05067 11 is RNA, reverse transcriptase can be used to synthesize a complementary DNA strand. The invention provides a process tor generating multiple copies of a single strand, circular nucleic acid template using the above polymerases.

The invention provides for polynucleotide primers which are selected so as to allow strand displacement synthesis by a polymerase. This displacement can be accomplished in a variety of ways. For example, primers can be chosen which are complementary over their entire sequence to the circular template. Extension of the primer around the template will result in displacement of the end of the primer by a polymerase. The resultant displacement product is a linear concatemer which is complementary to the circle template as shown in Figure If a completely complementary primer is used, then the polymerase selected should preferably be devoid of to 3' exonuclease activity, such as Klenow fragment. This selection prevents nick translation activity and ensures displacement synthesis.

Alternatively, polynucleotide primers can be selected in which the 5' end is non-complementary to sequences contained in the circle template, but the 3' end is complementary to contained sequences and hybridizes to the circle template. Extension of this primer-template aUsnd the circle results in displacement synthesis at the juncture of the hybridized and non-hybridized regions of primer.

Polynucleotide primers can also be selected wherein the 5' end of the nucleotide primers are blocked. Blocking of the 5' end of a primer, as defined above, allows for displacement synthesis. As with the 5' non-complementary primers, a primer modified at its 5' end by, for example, a chemical moiety, will not impede a polymerase's movement, nor will it be recognized by inherent 5' to 3' exonuclease PCI/US91/05067 WO 92/01813 12 activity found in most polymerases. Therefore, the invention provides a process for enerating multiple complements of a circular template wherein the 5' end of the nucleotide primers are non-complementary to the circular template or are blocked so as to allow displacement synthesis by a polymerase.

The invention further provides a process of synthesizing single strand, circular nucleic acids contcining between 30 and 750 nucleotides by chemically synthesizing a linear polynucleotide; combining the linear polynucleotide with a complementary link.ng oligonucleotide under conditions sufficient for hybridization; and ligating the linear polynucleotide to produce a single strand, circular nucleic acid. Ligation can be by enzymatic or chemical means.

The linear polynucleotides are chemically synthesized by methods known to those skilled in the art United States Patent No. 4,500,707, which is incorporated herein by reference). Current chemical synthesis methods may be used to synthesize linear polynucleotides of up to about 200 bases in length.

To generate a single strand, circular nucleic acid of about 200 nucleotides, a linear polynucleotide of about 200 nucleotides is first synthesized. The 5' and 3' ends of the linear molecules are brought together to form circular molecules by hybridization to complementary linking oligonucleotides Figure The linking oligonucleotides are designed such that the 5' end of the linking oligonucleotide is complementary to the 3' end of the synthesized linear polynucleotide and the remaining 3' end of the linking oligonucleotide is complementary to the end of the linear polynucleotide. The ends of the linear polynucleotide which have been brought together in this way are covalently joined by ligation. Alternatively, WO 92/01813 PCT/US91/05067 13 the use of RNA ligase without the use of linking oligonucleotides may be used to ligate a circle since RNA ligase is able to ligate DNA ends together. Additionally, larger single strand, circular nucleic acids composed of multiple linear polynucleotides of the same sequence may be achieved by varying the concentration of components in the ligation.

The invention also provides for the synthesis of single strand, circular nucleic acids, where the circular nucleic acid is synthesized from two or more linear polynucleotides Figure Synthesis of larger, single strand, circular nucleic acid templates can be accomplished, for example, by synthesizing two halves of the circle as linear polynucleotides. For example, to generate a 400 nucleotide circle, two shorter linear molecules whose sum is 400 nucleotides are first synthesized.

The linear molecules are joined together to create a circular molecule in the same fashion as that described above. Complementary linking oligonucleotides are designed to bring the 5' end of one linear molecule and the 3' end of the second linear molecule together. For example, to bring together two linear molecules which contain different terminal sequences at each of the four ends, two linking oligonucleotides are needed. One should be complementary to the 5' end of the first linear molecule and to the 3' end of the second molecule. The second linking oligonucleotide should therefore be complementary to the 3' end of the first molecule and to the 5' end of the second.

If the 5' and 3' ends of one linear molecule are identical or similiar in sequence to the 5' and 3' ends, respectively of the second linear molecule, then only a single complementary oligonucleotide may be used. Hybridization of the complementary oligonucleotides to the linear molecules will bring together the four ends of the WO 92/01813 PCT/US91/05067 14 molecules to form a non-covalently closed circle. The phosphodiester backbone can be ligated together to form covalently closed, single-stranded circles. In a similar manner, circles composed of three or more linear polynucleotide precursors can be produced. Additionally, alternative strategies for ligation of linear polynucleotides into circles may be employed and are shown in Figure 3. Therefore, the invention also provides for novel single strand, circular nucleic acid templates, wherein the template comprises between 30 and 750 nucleotides and is made from chemically synthesized oligoor polynucleotides.

The single strand, circular nucleic acid templates of the present invention can also be produced by a variety of other methods well known to those skilled in the art. For example, single strand circular templates can be derived through in vivo bacteriophage replication as described in Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, (1989); Ausubel et al., Current Protocols in Molecular Biology, John Wiley and Sons, (1987); and Short et al., Nucleic Acids Research 16:7583-7600 (1988), all of which are incorporated herein by reference. Alternatively, in vitro replication methods can be employed such as those described by Shavitt and Liuneh, Bacteriology, 171:3530-3538 (1989), which is 'ncorporated herein by reference. The sequence to be amplified can be cloned into a variety of available phage vectors, such as pBluescript' (Stratagene Cloning Systems, La Jolla, CA) or vectors can be constructed such that only pertinent sequences remain. The invention provides a process to generate multiple complements of a circular template, wherein the single strand, circular nucleic acid template is derived from phage replication.

Alternatively, circular nucleic acid templates can be generated by in vitro polymerase extension reactions. For WO92/01813 PCT/US91/05067 WO 92/01813 example, sequences of preferably between 50 and 2200 nucleotides to be transformed into a circula: template are first synthesized as a double-stranded duplex by priming a linear template and polymerase extension. Defined ends of linear duplex can be produced by digesting with restriction endonucleases known to cut at preselected sequences or by using preselected primers in polymerase chain reaction synthesis. The linear extension products can be circularized as a single-stranded molecule by first denaturing the duplex and hybridizing it to a oligonucleotide complementary to each end of the linear template. Treatment of this structure with ligase results in a single strand, circular template. Any linear molecules can be digested with DNases (such as Exo VII) to remove any residual linear fragments, including the complementary strand which does not ligate. Therefore, the invention provides a process to generate multiple complements of a circular template wherein the circular template is between 50 and 2200 nucleotides. Also provided are novel single strand, circular nucleic acid templates wherein the template is between 50 and 220 nucleotides.

Synthesis of circular nucleic acids can be accomplished using a combination of the methods uescribed above as well as in conjunction with methods known in the art. For example, methodology using bifunctional phosphorylating reagents as described by Capobianco et al., Nucleic Acids Research 18:2661-2669 (1990), which is incorporated herein by reference, can be employed for the synthesis of circular templates. This methodology is amenable to synthesis and circularization of nucleotides longer than four bases in length or can be used in conjunction with conventional phosphoramidite methodology to cyclize longer molecules such as between 50 and 750 nucleotides in length for chemically synthesized linear polynucleotides. Thus, the invention additionally provides a process to generate multiple copies of a single strand, WO 92/01813 PCT/US91/05067 16 circular template, wherein the template is chemically synthesized and is between 30 and 750 nucleotides.

In another aspect, the invention provides methods of characterizing small single-stranded circles 50-2200 bases in length, as are produced by any of the above methods.

Circularization of linear fragments produces nucleic acid products which migrate as bands with shifted electrophoretic mobilities, usually slower than the linear fragment. Such circles can be distinguished from the initial linear fragment(s) or from other linear by-products as follows: 1) by mobility shift relative to markers; 2) circles do not enzymatically degrade when treated with Exo VII (which requires free 3' or 5' ends); 3) circles can be primed and primers can be extended past the site of ligation; and 4) when annealed with unique restriction polynucleotides at a site other than the ligation site and restricted with the appropriate endonuclease, circles form only one full-length linear fragment. Any non-circular fragments, including the linkers used in producing the circle, will be degraded by Exo VII, cannot extend past the linear terminus and will produce at least two fragments when restricted.

The invention provides for a single strand, circular template which has at least one cleavage site. The cleavage sites are used, for example, to reduce the concatemeric displacement product down to individual linear complements. These individual complements correspond to a whole or partial single copy of the circle template.

Hybridization of a restriction polynucleotide or a singlestranded circle to the complementary displacement product produces a local duplex region which is the length of the restriction primer. The duplexed region is a substrate for endonucleolytic cleavage when treated with the appropriate endonuclease the endonuclease which recognizes the encoded restriction site). Since the cleavage site is 92/01813 PCT/US91/05067 WO 92/01813 17 encoded at a predetermined location, restriction of the site produces linear complements of defined length and sequence.

The invention also provides for a process to generate multiple complements of a single strand, circular template where the template has a restriction endonuclease site which is capable of being modified so as to inhibit restriction when hybridized in duplex form. For example, if the restriction endonuclease activity can be blocked with modified bases, then the appropriate restriction endonuclease will not cleave the DNA when one or both of the strands are modified. For example, endonucleases such as Hpa II, Hae II, Hae III and BstUl are known not to restrict at methylated sites. A particular use for this embodiment is depicted in Figure Restriction of the displacement product down to individual linear complements as described above will also restrict the circle template to a linear form unless the site on the template is refractory to digestion. For example, modification of a Hpa II site on the circle template by methylation as described by Nelson and McClelland, Nucleic Acids Research (1987), 15:219-230, and incorporated herein by reference, inhibits digestion of the hemi-methylated DNA and therefore preserves the structure of the circular template.

Preferably, the circular templates are methylated prior to initial hybridization with the polynucleotide primers by incorporation of modified bases such as 5-methylcytosine or methyladenosine into the linear polynucleotide during chemical synthesis as described by Ono and Ueda, Nucleic Acids Res. (1987) 15:219-232, which is incorporated herein by reference. The circle can also be methylated by a variety of other methods known to one skilled in the art.

Such methods include, for example, the use of specific methylase enzymes, such as Hpa II methylase, to methylate sites in the circle. Therefore, the invention additionally provides a process to generate multiple complements of a WO 92/01813 PCr/US91/05067 18 circular template wherein the restriction enzyme site on the template is modified by methylation. When it is not known if restriction enzyme activity can be blocked, simple procedures using oligonucleotide duplexes in solution can easily ascertain how much enzyme activity is reduced by site modification.

Restriction of the displacement product can be accomplished subsequent to, or preferably be performed simultaneously with, primer extension and displacement synthesis. In the former case, restriction endonucleases can be added at a later time. Displacement synthesis can even be stopped, for example, by heat denaturation of the polymerase or by organic extraction of the reaction.

Restriction polynucleotides are hybridized to the displacement product followed by digested with the appropriate restriction endonuclease. In the latter case, restriction polynucleotides and restriction endonucleases are included in the extension reactions. As the displacement product is produced, the restriction polynucleotides hybridize to the product and are concomitantly digested by the endonuclease (shown in Figure During simultaneous synthesis and restriction, if extension products resulting from primer extension of the hybridized restriction polynucleotides are undesirable, then restriction polynucleotides can be selected which cannot be used as substrates by a polymerase. These polynucleotides can be either the circular templates described herein which do not have free 3' terminal nucleotides for polymerization or linear molecules which are not substrates for polymerization. For example, polymerase recognition of a primer-template requires that about three terminal nucleotides of the primer be correctly hybridized to the template (Kornberg, DNA REPLICATION, W.H. Freeman and Company (1980), which is incorporated WO 92/01813 PCT/US91/05067 19 herein by reference). Therefore, the use of linear restriction polynucleotides which have non-complementary 3' terminal nucleotides will inefficiently promote polymerase extension reactions. Alternatively, the 3' end of the restriction polynucleotide can be chemically blocked to prevent polymerase extension. Addition of a blocking group such as chemical modification using a 2',3'-didedoxy nucleoside at the 3' end, reversing the orientation of the terminal nucleotide or covalently linking a non-hydroxy moiety such as an alkylamine will also allow the simultaneous synthesis and restriction of displacement product without resulting in extension products off of the restriction polynucleotides. Thus, the invention provides a process to generate multiple complements of a circular template where the restriction polynucleotides are deoxyribonucleic acids and the 3' end is inhibitory to primer extension by a polymerase.

The restriction polynucleotides can be further selected so that, once restricted, a fragment of the restriction polynucleotide remains hybridized to the end of the displacement product. The length of the end of the polynucleotide from the restriction site necessary to retain hybridization under various conditions and temperatures once restricted is known to one skilled in the art, or can be determined without undue experimentation.

These procedures are described in detail in Ausubel et al., ibid. Restriction polynucleotides selected in this way allow the linear complements produced after digestion to be used either directly or indirectly as a polynucleotide primer for further strand displacement synthesis. The hybridized portion of the restriction polynucleotide to the end of the linear complement functions in an analogous fashion as the non-complementary or blocked polynucleotide primers described above and therefore allows further hybridization to circular templates and polymerization.

WO 92/01813 PCT/US91/05067 The restriction polynucleotides can be further selected so that, once restricted, the fragment hybridized to the 3' end of the linear complement is unstable and does not remain hybridized. This restriction generates a single-stranded 3' end of the linear complement which is available to be recognized as a polymerase substrate when hybridized to a circular template. Again, polynucleotide lengths which are unstable under various conditions and temperatures are known or can be determined by one of ordinary skill in the art. For example, at 370C, which is the optimal temperature for a polymerase, a restriction primer which results in hybridization of only about four nucleotides once it is digested will be unstable.

Additionally, if modified circular templates are used as restriction primers, the digested 3' end of the linear complements are already hybridized to the circular templates and can be extended without rehybridization to a circular template. The use of circular templates in this way is advantageous if restriction of the circle is blocked. Thus, the invention provides for direct hybridization and primer-template formation between linear complements and single strand, circular template.

The invention also provides a process for generating multiple linear complements of a single strand, circular nucleic acid template containing at least one cleavage site by: combining the single strand, circular nucleic acid template with polynucleotide primers under conditions sufficient for hybridization; extending the polynucleotide primer more than once around the circle to generate a complementary displacement product comprising more than one contiguous complement of the single strand, circular nucleic acid template; cleaving the displacement product to generate linear complements of the circle template; combining the single strand, circular template of step with the linear complements produced in step under conditions sufficient for hybridization; WO 92/01813 PCT/US91/05067 21 repeating steps through at least once to generate linear complements of the circle template.

By subsequent hybridization, or preferably simultaneous hybridization, of the initially produced linear complements, the products of more than one cycle (steps a through c above which correspond to Figure 2, steps G through J) of linear complements can be generated.

Figure 2(K, parts 3-6) shows a schematic diagram depicting the hybridization of linear complements to single strand, circular templates and generation of additional displacement products and linear complements. Thus, repetitive cycles of priming, strand displacerent synthesis, cleaving of the linear complements and repriming with linear complement exponentially amplifies the copy number of the initial polynucleotide primer. Generation of multiple complements of the circle template indicates the presence of the initial polynucleotide primer.

The invention also provides a process for generating multiple linear complements of a single strand, circular template where all steps are carried out under isothermal conditions. Using the preferred embodiments of the present invention, all reactions including, for example, hybridization of polynucleotide primers, restriction polynucleotides and linear complement, strand displacement synthesis and restriction endonuclease digestion of displacement products can be performed at the same temperature. Preferably this temperature is between about and 70*C, more preferably between about 37'C to Isothermal conditions allow multiple and overlapping steps in the cycle to be performed rapidly and simultaneously.

Alternatively, the single strand, circular template can be denatured by heat prior to or during hybridization with linear complement. Heat denaturation ensures the availability of all templates for subsequent rounds of WO 92/01813 PCT/US91/05067 22 synthesis. Linear complements, once restricted, can also be denatured by heat prior to or during hybridization with circular template, ensuring the complete removal of the restriction polynucleotide for efficient primer-template hybridization.

The invention utilizes synthetic polynucleotide primers as well as synthetic restriction primers. Chemical synthesis of polynucleotide primers is well known to those skilled in the art. Methods disclosed herein for the synthesis of linear polynucleotides, such as phosphoramidite nucleotide chemistry, are routinely performed on automated synthesizers using protocols and reagents suggested by the manufacturers.

The invention further provides a process for generating multiple linear complements of a circular template where the polynucleotide primers comprise a mixture of double and/or single-stranded nucleic acids.

For example, the presence of a nucleic acid analyte within a biological sample can be determined by the processes disclosed herein. The analyte can be a nucleic acid from an infectious organism such as a virus or bacteria. The analyte can also be nucleic acid derived from a host organism such as a human. The detection of nucleic acids derived from almost any source allows the invention to be applicable in a large variety of clinical settings. Many infectious diseases and genetic disorders can be easily diagnosed using the invention.

Nucleic acids to be analyzed for such diseases and disorders can be isolated from the appropriate tissue or body fluid of an organism and used as polynucleotide primers in the generation of linear complements. If the analyte is RNA, the appropriate polynucleotide primers and their 3' ends for polymerase extension can be generated by first and second strand cDNA synthesis using preselected PCT/US91/05067 WO 92/01813 23 first or second strand primers. If the analyte is DNA, the appropriate 3' ends can be generated for primer extension by digesting the DNA with a preselected restriction enzyme.

Accordingly, any nucleic acid analyte can be used, or can be generated using ordinary skills in the art, so long as the 3' terminal nucleotides are sufficiently complementary to the circular template to form a primer-template.

In the process of the present invention, any 3'-ends of nucleic acids which are significantly complementary to the single-stranded circular nucleic acid may initiate synthesis of displacement products and linear complements.

If such nucleic acids are not the desired polynucleotide primer, such as an analyte target sequence, generation of non-specific background (false positive signal) may be produced. To decrease any non-specific background, the mixture of nucleic acids containing target sequences may be treated with nucleic acids complementary to some portion of the target. Such complementary nucleic acids or catcher nucleic acids may be immobilized on solid supports, such as beads or membranes, or contain a ligand, for example, biotin, which can be used to remove the hybridized catcher and target sequences from solution. After removal of any non-hybridized nucleic acids, the target fragment may now be used to prime the single-stranded, circular nucleic acid. Such affinity selection of target polynucleotide primer greatly reduces non-specific backgrounds.

If double-stranded nucleic acids, such as DNA or cDNA, are used or have been generated to use as polynucleotide primers, the strands are separated by denaturation prior to hybridization with the circular templates. Heat or alkaline denaturation are two common procedures for the separation of nucleic acid strands but a variety of other methods are known to one skilled in the art which can also be used. Detection of the analyte in the form of intermediary displacement products or in the form of linear WO 92/01813 PCT/US91/05067 24 complements can be performed using standard fluorescent staining or hybridization techniques known to one skilled in the art. The invention also provides a process for detecting multiple linear complements of a single strand, circular nucleic acid template containing at least one cleavage site by the steps of: combining the single strand, circular nucleic acid template with polynucleotide primers under conditions sufficient for hybridization; b) extending the polynucleotide primer more than once around the circle to generate a complementary displacement product comprising more than one contiguous complement of the single strand, circular nucleic acid template; cleaving the displacement product to generate linear complements of the circle template; combining the single strand, circular template of step with the linear complements produced in step under conditions sufficient for hybridization; repeating steps through at least once to generate linear complements of the circle template; and detecting the linear complements.

The invention further provides products such as DNA, RNA, polypeptides and antibodies produced from either the displacement products, displacement products hybridized to restriction polynucleotides, linear complements or linear complements hybridized to restriction polynucleotides.

These nucleic acids products, or nucleic acids encoding these products, can be cloned into a vector for propagation in either encaryotic or procaryotic organisms using ordinary skills in the art. The choice of the vector will depend on the organism in which the nucleic acid will be propagated. Additionally, the cloned products can be expressed in vivo for polypeptide production (using expression vectors) or they can be transcribed and (using transcription vectors) and translated in vitro to produce recombinant polypeptides. Such recombinant polypeptides derived by in vivo or in vitro methods can further be used to generate monoclonal or polyclonal antibodies as WO 92/01813 PCr/US91/05067 described by Harlow and Lane, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, 1988, which is incorporated herein by reference.

The following examples are intended to illustrate but not limit the invention.

EXAMPLES

Circular M13mpl8DNA (single or double stranded) was purchased from U.S. Biochemical (Cleveland, OH). All synthetic oligonucleotides were made using standard phosphoramidite chemistry on an Applied Biosystems 380A DNA synthesizer (Applied Biosystems, Foster City CA).

Purifications of DMT-on oligonucleotides was by "DMTon" reverse phase HPLC using 0.3 x 15 cm C-8 (31) columns eluting 5 to 30% acetonitrile in 100 mM triethyl-ammonium acetate at 1.0 ml/min. Phosphoramidites of 5-methyl-2' deoxycytidine and phosphorylating reagent were purchased from Glen Research, Herdon, VA. Where indicated, oligonucleotide linkers are synthesized with a blocked 3'end by synthesis on Branched Modifier Icaa-CPG (Glen Research), which results in oligomers with alkylamines on the 3'-hydroxyl. Size estimation of circles and extension products were based on mobility by agarose or polyacrylamide gel electrophoresis; size markers were either Hae III restricted double-stranded Phix174 or Hae III-restricted single-stranded M13 DNA. Total nucleic acid synthesis was quantitated by ionic adsorption of nucleic acid onto DE-81 disks after incorporation of 32

P_

dATP ci 32 P-dCTP using the method as described in Molecular Cloning: A Laboratory Manual, volume 3, 1988 Sambrook et al, eds, p. E18-E19. The number of primers extended in a reaction was quantitated by using 32P-end labeled primers of known specific activity.

The average number of complements produced from a WO 92/01813 PCT/US91/05067 26 orimed single stranded DNA circle was determined as follows: the circular single stranded DNA is primed by addition of a ten fold molar excess of complementary primer. A primer extension reaction was carried out on the bound primer in the presence of alpha- 32 P-dATP of known specific activity. The total amount of 32 P incorporated into circle complement was measured by spotting 2-5 gL of the reaction mixture onto Whatman DE81 ion exchange paper.

The DE81 paper was washed five times in 0.3M NaPO 4 pH for 5 minutes per wash to remove unincorporated 32 P-dATP.

The DE81 paper was counted by scintillation counting. The number of adenosine residues per circle complement and the circle concentration was known; allowing calculation of specific activity per circle complement and the number of circle complements produced per circle.

EXAMPLE I Extension of a 7300 Base Pair M13 Single-Stranded Circle to Produce Multiple Copies This example illustrates the ability of DNA polymerases to displace complement strands produced after extension and the ability of polymerases to incorporate several hundred bases per minute.

A 19 gl reaction volume contained 50 nM M13 model primer (sequence: 5'-dGGTTTTCCCAGTCACGACG) and 5 nM M13mpl8 single-stranded circular DNA Biochemical, Cleveland, OH) in Extension Buffer (10 mM Tris, pH 7.4, mM MgC1, 1 mM dithiothreitol (DTT)) containing 200 gM dGTP, dCTP, dTTP, and alpha- 32 P-dATP (6 pCi/pmol). The extension was initiated by addition of 1 Al containing 2 units of DNA polymerase Klenow fragment (BRL, Gaithersburg, MD).

Negative controls consisted of either no primer added or no enzyme added. The extension was incubated for one hour at either 37' or 50'C. Total DNA synthesis was determined by spotting 2 pl aliquots on DE81 anion exchange paper disks (Whatman, Clifton, NJ), and washed three times in 0.5 M WO 92/01813 PCT/US91/05067 27 sodium phosphate, pH 7.3. Aliquots of 2-5 pl were also analyzed by agarose gel electrophoresis followed by autoradiography on XAR film (Kodak, Rochester, NY) to estimate average size of fragments produced by the extension. The results indicated that the average size of circle complement produced was greater than 25,000 bases, or an extension rate of more than 417 bases per minute.

This represents more than 18,000 bases of displacement.

EXAMPLE II Construction of a 682-Base Single-Stranded Circle Based on M13 This example illustrates the construction of a medium size single-stranded circle using recombinant enzymatic methods.

One milligram of single-stranded M13mpl8 (U.S.

Biochemical, Cleveland, OH) was ethanol precipitated from 1500 1l total volume containing 300 mM sodium acetate, pH 5.4, and pelleted by centrifugation. The supernatent was removed, and the pellet washed with 70% ethanol and dried under vacuum. The DNA was redissolved in 500 pl water. A portion of the solution (90 Cl, 180 pg of DNA 76 pmols) was treated in the following manner: 1.5 p1 (750 pmols) of each of two restriction site oligonucleotides were added (500 pM Bgl II/M13 restriction site oligonucleotide sequence: 5'-dTGAGAGATCTACAAAGGCTATCAGGTCATTGCCT; AND pM Bam H1/M13 restriction oligonucleotide, sequence dTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCG); 15 p1 of Universal buffer (Stratagene Cloning Systems, La Jolla, CA) plus 27 Cl H20 was added. The mixture was heated to for 3 minutes, then cooled on ice for 5 minutes. 7.5 1l of Bam HI restriction endonuclease (Boehringer Mannheim, Indianapolis, IN) and 7.5 p1 of Bgl II restriction endonuclease (Boehringer Mannheim, Indianapolis, IN) was added and the mixture was incubated at 37*C for three hours.

WO 92/01813 PCT/US91/05067 28 After incubation, the digest was checked for completion on a 1.5% agarose mini-gel with approximately pg of the digested DNA. A preparative 1.5% agarose (FMC SeaKem GTG, Rockland, ME)/TAE was prepared with marker lanes. Agarose gel loading dyes (15.0 p1) were added to the digest. Ten p1 of this preparation were loaded in the marker lane and the remainder in the preparative well. The sample was electrophoresed in TAE buffer for 1.5 hours at mA.

The marker lane was cut from the gel and stained with ethidium bromide in water at a concentration of 0.5 pg/ml.

The 682 bp fragment was located under UV light and then excised from the corresponding location on the gel.

The gel slice was loaded onto a Schleicher Schuell Elutrap (Schleicher Schuell, Keene, NH) and electroeluted in Ix TAE buffer for 4 hours at 130 V. The eluate was recovered and butanol extracted 4 times, Phenol/chloroform/isoamyl alcohol extracted twice and chloroform/isoamyl alcohol extracted one time. One tenth volume of 3 M sodium acetate pH 5.4 and 2.5 volumes of 100% ethanol were added and the preparation was incubated in an ethanol/dry ice bath for 20 minutes. The preparation was then centrifuged in a microfuge for 30 minutes at room temperature at 14,000 X G. The supernate was discarded, 0.75 ml of 70% ethanol was added to wash the pellet and the preparation was then re-centrifuged for 5 minutes under the same conditions. Ethanol was removed and the pellet was dried under vacuum for 3 minutes.

The dried pellet was redissolved in 100 1l of H 2 0 and loaded onto a Centricon 100 (Amicon, Danvers, ME). Two mls of 50 mM NaOH/1 mM EDTA were added and the preparation was spun in a fixed angle rotor, (Model SS34; Dupont-Sorvall, Wilmington, DE) centrifuged for 45 minutes at room WO 92/01813 PCT/US91/05067 29 temperature at 2400 rpm. The above steps were repeated with an additional two mls of NaOH/EDTA. One ml of TE 10:1 pH 8.0 (10 mM Tris pH 8.0, 1 mM EDTA) was added and centrifuged as above.

Circularization of the 682 bp fragment was performed as follows: The retentate was recovered from Centricon 100 filter apparatus at a total volume of 38 pl. Forty 1p of ligase buffer (BRL, Gaithersburg, MD) were added. A linker molecule to span both ends of the linear 682 bp fragment was synthesized as described above. The synthesized linker molecule, designated M13/682 liGation linker, had the following sequence: One hundred and seven pl of H 2 0 were added and the preparation was incubated at 65*C for 5 minutes, followed by flash cooling on ice for minutes. Three Al of T4 DNA Ligase (BRL, Gaithersburg, MD) containing lU/pl were added and the preparation was incubated at 16'C for 1 hour. Following incubation, two additional ls of linker were added. The preparation was incubated at 65*C for 5 minutes, cooled on ice 5 minutes, and then three pls of ligase were added followed by a 1 hour incubation at 16'C. Two additional ils of linker were added, followed by another 65'C incubation, rapid cooling on ice for 5 minutes. Three more ils of ligase were added and reaction was incubated at 16C overnight.

EXAMPLE III Construction of a Synthetic 72-Base Single-Stranded Circle with Protected Restriction Sites and a Single Core Sequence This example illustrates the construction of a small single-stranded circle using oligonucleotide synthesis followed by enzymatic ligation to form the circle.

A 72 base DNA oligomer with a 5'-phosphate sequence: -p-d CTTTCACGTTTATCATCTGACTATCCTGTAATAAAGATCAATGCGTCC WO 92/01813 PCT/US91/05067 GGTCTACATAGTCTCTAAAATTAG) was synthesized on an Applied Biosystems 380A DNA synthesizer (Applied Biosystems, Foster city, CA) using standard phosphoramidite chemistry. Both deoxycytidines in the HpaII restriction site (CCGG) contained within the 72 base DNA oligomer were replaced with 5-methyldeoxycytidine to prevent cleavage of the oligomer by Hpa II. A 33 base linker sequence that has bases complementary to each end of the 72 base oligomer was also synthesized. The 3' end of the linker has 3 noncomplementary bases and a blocked 3' end to prevent the linker from extending in a polymerase reaction. This oligomer was used to bring the 5' and 3' ends of the 72 base oligomer together to allow for enzymatic ligation.

The 72 base oligomer was enzymatically ligated in a ImL reaction volume containing 10uM of 72 base oligomer, of 33 base linker oligomer, 40mM Tris-Cl pH 7.5, MgCl 2 10mM dithiothreotol, and 1mM ATP. The reaction mixture was incubated for 5 minutes at 50 0 C then cooled to room temperature. 40,000 units of T4 DNA Ligase (New England Biolabs, Beverly, MA) was added to the reaction mixture. The reaction mixture was incubated for 2 hours at room temperature. The linker concentration was brought to and the reaction mixture was incubated an additional 16 hours at room temperature. The linker concentration was increased to 15uM and the reaction mixture was incubated an additional 2 hours at room temperature. The reaction mixture was electrophoresed at 1000V for 4 hours in a polyacylamide/8M urea gel in IxTBE The slower migrating circular 72mer product was identified by UV shadowing, electroeluted, ethanol precipitated and quantitated by optical density at 260nm. The circular 72mer was resistant to degradation by Exo VII, and could be primer extended past the ligation site.

WO 92/01813 PCTiUS91/05067 31 EXAMPLE IV Construction of a Synthetic 101-Base Single-Stranded Circle with Protected Restriction Sites and a Single Core Secquence A synthetic 101mer oligomer (sequence: pdGATTAAAATCTCTGACCCGAGCCGATGACTTACTGGCG'CGTTmCGAACCGGA AAGCTCCCTGGAGTGCGATCTTCCTGAGGCCGATCTGATAGGACATTAT) was synthesized as in Example III. The underlined portion designates the sequence which is complementary to the 33 base linker described below. The 101mer oligomer contained blocked restriction sites for the restriction endonucleases Hpa II (CCGG), Bst B1 (TTCGAA), and Bst Ul (CGCG). All the deoxycytosines in the restriction sites of these restriction endonucleases were replaced with methyldeoxycytosines to prevent restriction cleavage. A 33 base linker (sequ ence: 5' dTCAGAGATTTTAATCATAATGTCCTATCAGTAG) that has 15 bases complementary to each end of the 101 base oligomer was also synthesized. The 3' end of the linker has 3 noncomplementary bases and a 3' amine to prevent the linker from extending in a polymerase reaction. This oligomer was used to bring the 5' and 3' ends of the 101 base oligomer together to allow for enzymatic ligation. The 101 base oligomer was enzymatically ligated and purified as in Example III. The circular 101mer was resistant to degradation by Exo VII and could be primer extended past the site of ligation.

EXAMPLE V Construction of a Synthetic 202-Base Single-Stranded Circle with Protected Restriction Sites and a Repeated Core Sequence This example illustrates the construction of a small single-stranded circle composed of two core sequences with unique ends enzymatically ligated to form the circle.

A synthetic 101 base oligomer (sequence: WO 92/01813 PCT/US91/05067 32 pdTTCTAGTTACGCAGGCCCGAGCCGATGACTTACTGGCCGCGTTCGAACCGGAAAG CTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTATTTGCACTTTC) was synthesized as in Example III with the sama 71 base core sequence but with different 15 base linker sequences on each end of the 101 base oligomer. The different linker sequences, which are underlined, allow for specific ligation between the 101 base oligomer shown above and the 101 base oligomer from Example IV. Two 34 base linkers (sequences: NH2 and 5'-dTCAGAGATTTTAATCGAAAGTGCAAATAGTCCCC-NH 2 that have 15 bases complementary to one end of each 101 base oligomer were also synthesized. The 3' end of the linkers have 4 noncomplementary bases and a 3' amine to prevent the linkers from extending in a polymerase reaction. Each linker wwas used to bring the 5' end of one 101 base oligomer and 3' end of the other 101 base oligomer together to allow for enzymatic ligation.

The 101 base oligomers were enzymatically ligated and purified into a 202 base oligomer as in Example III except that each 101 base oligomer was at 0.6 AM and the first linker was at 0.15 pM starting concentration and increased to 0.75 pM. The 101 base oligomers were also gel purified before ligation instead of after ligation. The 202 base oligomer was then ligated into a circle as in Example III except the 202 base oligomer was at 0.3 pM and the second linker was at 0.15 pM starting concentration and increased to 1.2 M.

EXAMPLE VI Extension of a 682-Base Single-Stranded Circle This example illustrates the ability of DNA polymerases to displace complement strands produced after extension, and the ability of polymerases to incorporate several hundred bases per minute into a medium-sized single-stranded circle. A primer extension reaction was WO 92/01813 PCT/US91/05067 33 carried out and extension products determined as described in Example I. Extension reactions for the circular 682 bp single strand, nucleic acid were performed using free nucleotides, a DNA Polymerase (BRL, Gaithersburg, MD), a synthetic 32 P labeled primer fragment (5000 cpm/fmole) and T4 Polynucleotide Kinase (New England Biolabs, Beverly, MA) The primer sequence was 5'TGAACGGTAATCGTAAAACTAGCATGTCAATCATATGTACCCCGGTTGAT3'.

Reactions were performed in 20 gl volumes with a 10:1 molar ratio of primer to circle, dithiothreotol (DTT) at a final concentration of 5 mM, free dNTP's (Pharmacia, Pleasant Hill, CA) at a final concentration of 200 PM, Klenow DNA polymerase (Boehringer Mannheim, Indianapolis, IN), and extension buffer. Reactions were incubated at 37*C for one hour. Three and a half pls of 6X alkaline gel loading buffer were added to the reactions and then electrophoresis was carried out in a 0.7% alkaline agarose gel for 16 hours at 150 mA. The gel was neutralized and stained as described above and then exposed on Kodak X-OMAT AR film (Kodak, Rochester, NY) at room temperature with a screen for two hours to analyze extension products. The average size of circle complement produced using a 682-base singlestranded circle was greater than 20,000 bases.

EXAMPLE VII Extension of a 72-Base Circle A primer extension reaction was carried out as in Example I except a different primer was used (sequence: The 19 bases on the 3' end are complementary to the 72 base circle but the 18 bases or the 5' end are not complementary to the circle. The average size of circle complement produced using a 72-base single-stranded circle was 150 to 250 bases.

WO 92/01813 PCT/US91/05067 34 EXAMPLE VIII Extension of a 7300-Base Single-Stranded Circle, and Restriction to Form Secondary Primers This example illustrates the ability of Sau 3A endonuclease to restrict the amplified product of a 7300base single-stranded circle to generate linear complements for use as secondary primers in the next cycle of extension reaction.

A 19 pl reaction volume contained 50 nM M13mpl8 single-stranded circle Biochemical, Cleveland, OH) and 5 nM M13 primer (100 fmole) in extension buffer (20 mM Tris pH 7.5, 10 mM MgCl 2 25 mM NaC1, 6.5 mM DTT) containing 200 pM dGTP, dCTP, dATP, dTTP and 0.33 pM of alpha 32

P-

dATP. The final specific activity of 3P-dATP was 9,000 cpm/pmole. The extension was initiated by addition of 1 1l of DNA polymerase Klenow fragment (BRL, Gaithersburg, MD, 6 units/pl) for 1 hour at 50*C. 2 pl1 of 8 units/il Sau3A I enzyme (BRL, Gaithersburg, MD) were added to the 20 p1 reaction mix and allowed to digest for 1 hour at 370C. 4 p1 of the 20 1l reaction mix was transferred into a new tube and denatured for 2 minutes at 100"C in a heat block (silicon oil/sand or boiling water), followed by chilling in a dry ice bath for 1 minute. 16 pl1 of new reaction mix containing 40 nM M13mpl8 single-stranded circle and the same concentration of extension buffer mentioned above was added to the 4 pl chilled sample. 1 p1 of DNA polymerase Klenow fragment was added and the extension reaction was carried out for 1 hour at 50*C. The restriction/denaturation/extension cycles were repeated two more times. The restricted and extended products were analyzed by gel and total DNA synthesis. 2 pl of each sample tube was loaded onto a 0.5% alkaline agarose denaturing gel and run for overnight at 150 mA. A 1:10 diluted stock of each sample tube was prepared and 2 1l was used to spot onto DE81 Whatman paper, washed 3 times in WO 92/01813 PCT/US91/05067 sodium phosphate, pH 7.3 and counted in a scintillation counter for total count analysis. Denatured, extended samples were used as controls. Results indicated that the extended product was restricted and the restricted fragments in turn acted as primers for the next round of extension. Since each molecule of M13mpl8, (complement contains about 1760 adenine residues, incorporation of 9,000cpm/pmol 3p-dATP will result in complement product with specific activity of 16,000 cpm/fmol. Results (E=extension; R=restriction; D=heat denaturation) cpm fmols fold Reaction incorporated complement increase E only 6.4 x 10 6 400 4 E/R 2 x 107 1,250 12.5 E/R/O/E 2 x 108 12,500 125 E/R/D/E/R 3 x 108 18,750 187.5 E/R/D/E/R/D/E 2.2 x 109 137,500 1,375 These results indicate that 1,375 molecules of circle complement are formed for every original molecule of polynucleotide primer, or a 1,375 fold amplification over original target polynucleotide. Repeating the reaction under isothermal conditions at 50C gave similar results.

EXAMPLE IX Extension of a 72-Base Single-Stranded Circle, and Restriction to Form Secondary Primers A primer extension reaction was carried out as in Example VII except Taq DNA Polymerase (Cetus, Emeryville, CA) was used at 60*C instead of Klenow at 37*C. The extended product was brought to 5 mM in DTT and 1 pM in a 38 base restriction linker (sequence: dTGACTATCCTGTAATAAAGATCAATGCGTCCGGTCTAC-NH 2 The restriction linker is complementary to the circle complement and renders it double-stranded. 10 units of Hpa PCI/US91/05067 WO 92/01813 II restriction endonuclease were was incubated 30 minutes at visualized by autoradiography.

cleaved into 72 base fragments Sadded and the reaction mix 37'C. The product was The circle complement was following this procedure.

Although the invention has been described with references to the presently preferred embodiment, it should be understood that various modifications can be made without departing from the spirit of the invention.

Accordingly, the invention is limited only by the claims.

Claims (36)

1. A process for generating multiple linear complements of a single strand, circular nucleic acid template containing at least one cleavage site comprising: combining the single strand, circuar nucleic acid template with polynucleotide primers under conditions sufficient for hybridization; and extending the polynucleotide primer more than once around the circle to generate a complementary displacement product comprising more than one contiguous complement of the single strand, circular nucleic acid template. WO 92/01813 PCT/US91/05067 38

2. A process for generating multiple linear complements of a single strand, circular nucleic acid template containing at least one cleavage site comprising: combining the single strand, circular nucleic acid template with polynucleotide primer under conditions sufficient for hybridization; extending the polynucleotide primer more than once around the circle to generate a complementary displacement product comprising more than one contiguous complement of the single strand, circular nucleic acid template; cleaving the displacement prodct to generate linear complements of the circle template; combining the single strand, circular template of step with the linear complements produced in step under conditions sufficient for hybridization; and repeating steps through at least once to generate linear complements of the circle template wherein the last repeated cycle ends at step or

3. The process of claim 2, wherein the circular template of step is denatured prior to use in step WO 92/01813 PCIAIS91~;L/05067 39

4. The process of claim 2, wherein the product of step is denatured prior to use in step The process of claim 1 or 2, wherein all steps are carried out under isothermal conditions.

6. The process of claim 1 or 2, wherein the polynuclec-:ide primers comprise double-stranded nucleic acids, single-stranded nucleic acids or a mixture thereof.

7. The process of claim 6, wherein the nucleic acids are derived from a biological sample.

8. The process of claim 6, wherein the polynucleotide primers are double-stranded and their strands are separated by denaturing before or during step

9. The process of claim 8, wherein the denaturing is thermal. The process of claim 1 or 2, wherein the polynucleotide primers are synthetic oligonucleotides.

11. The process of claim 1 or 2, wherein the 1'olynucleotide primers are selected so as to allow strand displacement synthesis by a polymerase.

12. The process of claim 1 or 2, wherein the end of the polynucleotide primers are non-complementary to the circular template.

13. The process of claim 1 or 2, wherein the end of the nucleotide primers are blocked. WO 92/01813 i'Cr/ US')/05067

14. A process of synthesizing a single strand, circular nucleic acid comprising: chemically synthesizing a linear polynucleotide; combining the linear polynucleotide with a complementary linking oligonucleotide under conditions sufficient for hybridization; and ligating the linear polynucleotide to produce a single strand, circular nucleic acid. The process of claim 14, wherein the circular nucleic acid is synthesized as two or more linear polynucleotides.

16. A single strand, circular nucleic acid, wherein the nucleic acid comprises between 30 and 2200 nucleotides.

17. The single strand, circular nucleic acid of claim 16, wherein the nucleic acid comprises between 30 and 750 nucleotides.

18. The single strand, circular nucleic acid of claim 17, wherein the nucleic acid comprises between 50 and 300 nucleotides.

19. The single strand, circular nucleic acid of claim 18, wherein the circular nucleic acid further comprises at least one cleavage site. WO 92/01813 PCG/US91/05067 41 The single strand, circular nucleic acid of claim 19, wherein a portion of the circular nucleic acid is complementary to polynucleotide primers derived from a biological sample comprising double-stranded nucleic acids, single-stranded nucleic acids or a mixture thereof.

21. The process of claim 1 or 2, wherein the single strand, circular nucleic acid template is derived from phage replication.

22. The process of claim 1 or 2, wherein the single strand, circular nucleic acid template is between and 2200 nucleotides.

23. The process of claim 1 or 2, wherein the single strand, circular nucleic acid template is made from chemically synthesized oligo- or poly-nucleotides.

24. The process of claim 23, wherein the single strand, circular nucleic acid template is between 50 and 750 nucleotides. The process of claim 1 or 2, wherein the cleaving of the displacement product is achieved by one or more restriction endonucleases.

26. The process of claim 25, wherein the one or more restriction endonuclease sites on the single strand, circular nucleic acid template are capable of being modified so as to inhibit restriction of at least the modified strand.

27. The process of claim 25, wherein the restriction endonuclease site is Hpa II. WO 92/01813 PCr/US91/05067 42

28. The process of claim 26, wherein the one or more restriction endonuclease sites are modified by methylation.

29. The process of claim 1 or 2, wherein the restriction polynucleotides are selected so that when restricted, a fragment of the restriction polynucleotide remains hybridized to the 5' end of the displacement product. The process of claim 1 or 2, wherein the restriction polynucleotides are deoxyribonucleic acids and the 3' end is inhibitory to primer extension by a polymerase.

31. The process of claim 1 or 2, wherein the polymerase is selected from the group comprising E. coli DNA polymerase I, Klenow fragment, T4 DNA polymerase, T7 DNA polymerase, modified polymerases, reverse transcriptase, Taq polymerase, Bst polymerases or T. flavins polymerase. WO 92/01813 PC/US91/05067 43

32. A process for detecting multiple linear complements of a single strand, circular nucleic acid template containing at least one cleavage site comprising: combining the single strand, circular nucleic acid template with polynucleotide primers under conditions sufficient for hybridization; extending the polynucleotide primer more than once around the circle to generate a complementary displacement product comprising more than one contiguous complement of the single strand, circular nucleic acid template; detecting the linear complements. WO 92/01813 PCr/US91/05067 44

33. A process for detecting multiple linear complements of a single strand, circular nucleic acid template containing at least one cleavage site comprising: combining the single strand, circular nucleic acid template with polynucleotide primers under conditions sufficient for hybridization; extending the polynucleotide primer more than once around the cirrle to generate a complementary displacement product comprising more than one contiguous complement of the single strand, circular nucleic acid template; cleaving the displacement product to generate linear complements of the circle template; combining the single strand, circular template of step with the linear complements produced in step under conditions sufficient for hybridization; repeating steps through at least once to generate linear complements of the circle template, wherein the last repeated step ends at step or and detecting the linear complements.

34. The process of claim 32 or 33, wherein the detecting step is by hybridization. WO 92/01813 iPcr/USgi/05067 The process of claim 32 or 33, wherein the detecting step is by fluorescence.

36. The process of claim 32 or 33, wherein the polynucleotide primers comprise double-stranded nucleic acids, single-stranded nucleic acids or a mixture thereof.

37. The process of claim 36, wherein the nucleic acids are derived from a biological sample.

38. The displacement products produced by the process of claim 1 or 2.

39. The displacement products hybridized to the restriction polynucleotides produced by the process of claim 1 or 2. The linear complements produced by the process of claim 1 or 2.

41. A product made with the product of claim 38 selected from the group consisting of DNA, RNA, polypeptide and antibody.

42. A product made with the product of claim 39 selected from the group consisting of DNA, RNA, polypeptide and antibody.

43. A product made with the product of claim selected from the group consisting of DNA, RNA, polypeptide and antibody.

44. The process of claim 1 further comprising step cleaving of the displacement product to generate linear complements of the circle template. WO 92/01813 Pcr/US9J /05067 The process of claim 1 or 2, wherein the polynucleotide primers are affinity selected.