AU6045196A - Secretion leader trap cloning method - Google Patents
Secretion leader trap cloning methodInfo
- Publication number
- AU6045196A AU6045196A AU60451/96A AU6045196A AU6045196A AU 6045196 A AU6045196 A AU 6045196A AU 60451/96 A AU60451/96 A AU 60451/96A AU 6045196 A AU6045196 A AU 6045196A AU 6045196 A AU6045196 A AU 6045196A
- Authority
- AU
- Australia
- Prior art keywords
- cloning method
- secretion leader
- trap cloning
- trap
- leader
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/32—Fusion polypeptide fusions with soluble part of a cell surface receptor, "decoy receptors"
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US47280695A | 1995-06-07 | 1995-06-07 | |
US48349395A | 1995-06-07 | 1995-06-07 | |
US483493 | 1995-06-07 | ||
US472806 | 1995-06-07 | ||
PCT/US1996/008936 WO1996040904A1 (en) | 1995-06-07 | 1996-06-04 | Secretion leader trap cloning method |
Publications (1)
Publication Number | Publication Date |
---|---|
AU6045196A true AU6045196A (en) | 1996-12-30 |
Family
ID=27043933
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU60451/96A Abandoned AU6045196A (en) | 1995-06-07 | 1996-06-04 | Secretion leader trap cloning method |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0832209A1 (en) |
JP (1) | JPH11506939A (en) |
AU (1) | AU6045196A (en) |
CA (1) | CA2223155C (en) |
WO (1) | WO1996040904A1 (en) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5536637A (en) * | 1993-04-07 | 1996-07-16 | Genetics Institute, Inc. | Method of screening for cDNA encoding novel secreted mammalian proteins in yeast |
GB9701492D0 (en) * | 1997-01-24 | 1997-03-12 | Univ Edinburgh | Transfection of embryonic stem cells |
WO1999005256A2 (en) * | 1997-07-24 | 1999-02-04 | President And Fellows Of Harvard College | Method for cloning secreted proteins |
JP3499528B2 (en) | 1997-11-26 | 2004-02-23 | 中外製薬株式会社 | Signal sequence trap method |
US6103472A (en) * | 1998-02-20 | 2000-08-15 | Amgen Inc. | Methods and compositions for identifying novel secreted mammalian polypeptides in yeast |
US6150098A (en) * | 1998-02-20 | 2000-11-21 | Amgen Inc. | Methods for identifying novel secreted mammalian polypeptides |
WO1999049028A1 (en) * | 1998-03-23 | 1999-09-30 | Genentech, Inc. | Method of selection for genes encoding secreted and transmembrane proteins |
WO1999060113A1 (en) | 1998-05-20 | 1999-11-25 | Chugai Seiyaku Kabushiki Kaisha | Novel method for isolating gene |
WO2001009310A1 (en) * | 1999-07-29 | 2001-02-08 | Berlex Laboratories, Inc. | 5'ENRICHED cDNA LIBRARIES AND A YEAST SIGNAL TRAP |
US7112434B2 (en) | 2001-05-02 | 2006-09-26 | University Of South Florida | Vector system for selection of genes encoding secreted proteins and membrane-bound proteins |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2061482T3 (en) * | 1986-05-02 | 1994-12-16 | Gist Brocades Nv | METHOD FOR THE IDENTIFICATION OF SECRETORY SIGNAL SEQUENCES FOR SYNTHESIS OF EXTRACELLULAR PROTEIN IN BACILLUS. |
US5817797A (en) * | 1988-06-01 | 1998-10-06 | The United States Of America As Represented By The Department Of Health And Human Services | Sequencing DNA; a modification of the polymerase chain reaction |
DE3901681A1 (en) * | 1989-01-21 | 1990-07-26 | Behringwerke Ag | SIGNAL PEPTIDE FOR THE SECRETION OF PEPTIDES IN ESCHERICHIA COLI, METHOD FOR ITS EFFECT AND ITS USE |
GB8926269D0 (en) * | 1989-11-21 | 1990-01-10 | Dynal As | Plasmid |
JP2879303B2 (en) * | 1993-01-14 | 1999-04-05 | 佑 本庶 | Method for preparing cDNA library, novel polypeptide and DNA encoding the same |
DE4332463A1 (en) * | 1993-09-24 | 1995-03-30 | Boehringer Mannheim Gmbh | Process for the specific cloning of nucleic acids |
-
1996
- 1996-06-04 AU AU60451/96A patent/AU6045196A/en not_active Abandoned
- 1996-06-04 CA CA002223155A patent/CA2223155C/en not_active Expired - Fee Related
- 1996-06-04 JP JP9501386A patent/JPH11506939A/en active Pending
- 1996-06-04 WO PCT/US1996/008936 patent/WO1996040904A1/en not_active Application Discontinuation
- 1996-06-04 EP EP96918107A patent/EP0832209A1/en not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
JPH11506939A (en) | 1999-06-22 |
CA2223155C (en) | 2003-08-12 |
CA2223155A1 (en) | 1996-12-19 |
WO1996040904A1 (en) | 1996-12-19 |
EP0832209A1 (en) | 1998-04-01 |
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