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ATE514770T1 - Verwendung von pilzmutanten zur expression von antikörpern - Google Patents

Verwendung von pilzmutanten zur expression von antikörpern

Info

Publication number
ATE514770T1
ATE514770T1 AT06791474T AT06791474T ATE514770T1 AT E514770 T1 ATE514770 T1 AT E514770T1 AT 06791474 T AT06791474 T AT 06791474T AT 06791474 T AT06791474 T AT 06791474T AT E514770 T1 ATE514770 T1 AT E514770T1
Authority
AT
Austria
Prior art keywords
endogenous
activity
protease activity
kexin
express antibodies
Prior art date
Application number
AT06791474T
Other languages
English (en)
Inventor
Mogens Trier Hansen
Christian Isak Jyrgensen
Jan Lehmbeck
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE514770T1 publication Critical patent/ATE514770T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT06791474T 2005-10-17 2006-10-17 Verwendung von pilzmutanten zur expression von antikörpern ATE514770T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200501452 2005-10-17
PCT/DK2006/000586 WO2007045248A1 (en) 2005-10-17 2006-10-17 Use of fungal mutants for expression of antibodies

Publications (1)

Publication Number Publication Date
ATE514770T1 true ATE514770T1 (de) 2011-07-15

Family

ID=37496608

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06791474T ATE514770T1 (de) 2005-10-17 2006-10-17 Verwendung von pilzmutanten zur expression von antikörpern

Country Status (6)

Country Link
US (1) US7858360B2 (de)
EP (1) EP1941025B1 (de)
CN (1) CN101292024B (de)
AT (1) ATE514770T1 (de)
DK (1) DK1941025T3 (de)
WO (1) WO2007045248A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007535913A (ja) * 2004-01-21 2007-12-13 ノボザイムス アクティーゼルスカブ ヘテロカリオン菌類又は菌類宿主細胞におけるモノクローナル抗体の生成
US8383391B2 (en) 2007-10-01 2013-02-26 Novozymes A/S Mutant cell with deleted or disrupted genes encoding protease
WO2010059413A2 (en) 2008-11-20 2010-05-27 Novozymes, Inc. Polypeptides having amylolytic enhancing activity and polynucleotides encoding same
JP5305353B2 (ja) * 2009-06-23 2013-10-02 ヤマサ醤油株式会社 ａｌｐＢをコードする遺伝子の機能が欠損している麹菌及びその利用
CN111500474B (zh) * 2009-12-18 2023-10-03 诺维信股份有限公司 用于在木霉属的蛋白酶缺陷突变体中产生多肽的方法
WO2011161206A1 (en) 2010-06-25 2011-12-29 Novozymes A/S Polynucleotides having leader sequence function
WO2011161207A1 (en) 2010-06-25 2011-12-29 Novozymes A/S Polynucleotides having promoter activity
EP2585601A1 (de) 2010-06-25 2013-05-01 Novozymes A/S Polynukleotide mit promoterwirkung
WO2012075151A2 (en) 2010-11-30 2012-06-07 Novozymes, Inc. Promoters for expressing genes in a fungal cell
CN102174453A (zh) * 2010-12-01 2011-09-07 江南大学 一种产碱性蛋白酶基因工程菌及其应用
CA2820248C (en) 2010-12-07 2019-10-29 Vnomics Corp. System and method for measuring and reducing vehicle fuel waste
FI123425B (fi) * 2011-03-31 2013-04-30 Ab Enzymes Oy Proteaasientsyymi ja sen käytöt
EP2527448A1 (de) 2011-05-23 2012-11-28 Novozymes A/S Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze
EP2527432A1 (de) 2011-05-23 2012-11-28 Novozymes A/S Bidirektionaler Cytosin-Deaminase-codierender Selektionsmarker
CN103621188B (zh) * 2011-06-15 2017-08-08 哈利伯顿能源服务公司 粗大硬金属颗粒内注射焊枪以及相关的组合、系统和方法
US20130084608A1 (en) 2011-09-30 2013-04-04 Codexis, Inc. Fungal proteases
CA2861697C (en) 2012-01-05 2022-03-15 Novartis Ag Protease deficient filamentous fungal cells and methods of use thereof
US9695454B2 (en) 2012-05-23 2017-07-04 Glykos Finland Oy Production of fucosylated glycoproteins
KR102111078B1 (ko) * 2013-07-04 2020-05-18 노파르티스 아게 O-만노실트랜스퍼라제 결핍 사상균 세포 및 이의 이용 방법
DK3019602T3 (en) 2013-07-10 2018-11-12 Glykos Finland Oy MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
CN103451173B (zh) * 2013-08-21 2015-01-28 山东省农业科学院生物技术研究中心 通过单点突变缺失结构中的盐键提高pae酶活的方法
US10619144B2 (en) 2013-08-23 2020-04-14 Novozymes A/S Regulated PepC expression
US10513724B2 (en) 2014-07-21 2019-12-24 Glykos Finland Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
US10430838B1 (en) 2016-06-28 2019-10-01 Snap Inc. Methods and systems for generation, curation, and presentation of media collections with automated advertising
WO2018167153A1 (en) 2017-03-17 2018-09-20 Novozymes A/S Improved filamentous fungal host cell
CN110724644A (zh) * 2019-12-05 2020-01-24 西北农林科技大学 一种产重组人胰岛素原的里氏木霉工程菌及其应用
IL300173A (en) 2020-07-31 2023-03-01 Biotalys NV Expression host
WO2022204576A1 (en) * 2021-03-25 2022-09-29 Bio-Cat, Inc. Fungal protease mixtures and uses thereof
CN113151329B (zh) * 2021-03-30 2023-09-08 云南师范大学 中性蛋白酶突变体及其构造方法和应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5190931A (en) 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US4885249A (en) 1984-12-05 1989-12-05 Allelix, Inc. Aspergillus niger transformation system
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
DE69333249T2 (de) * 1992-04-15 2004-07-29 Novartis Ag Neue Schimmel-Protease
DE69534185T2 (de) 1994-06-30 2006-02-23 Novozymes Biotech, Inc., Davis Nicht-toxisches, nicht-toxigenes, nicht-pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
NL1000803C2 (nl) 1995-07-14 1997-01-15 Ambrosiushoeve Proefbedrijf Werkwijze voor het telen van hommelkoninginnen en werkwijze voor het telen van hommels.
CN1160465C (zh) * 1995-12-15 2004-08-04 诺沃奇梅兹有限公司 其中areA、pepC和/或pepE基因已被灭活的真菌
AU2152897A (en) 1996-03-27 1997-10-17 Novo Nordisk A/S Alkaline protease deficient filamentous fungi
ES2256895T3 (es) 1996-09-19 2006-07-16 Novozymes A/S Celulas huesped y metodos de produccion de proteinas.
ATE414782T1 (de) 1998-12-23 2008-12-15 Novozymes As Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen
CN1940067A (zh) 1999-03-22 2007-04-04 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
EP1175496A1 (de) 1999-05-06 2002-01-30 Aventis Animal Nutrition S.A. Rekombinante penicillium funiculosum zur homologen und heterologen proteinherstellung
DE60142226D1 (de) 2000-03-14 2010-07-08 Novozymes As Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
US7063962B2 (en) 2001-07-20 2006-06-20 Novozymes A/S DNA sequences for regulating transcription
US20040018573A1 (en) * 2002-04-18 2004-01-29 Power Scott D Production of functional antibodies in filamentous fungi

Also Published As

Publication number Publication date
DK1941025T3 (da) 2011-09-26
EP1941025B1 (de) 2011-06-29
CN101292024A (zh) 2008-10-22
US7858360B2 (en) 2010-12-28
WO2007045248A1 (en) 2007-04-26
EP1941025A1 (de) 2008-07-09
CN101292024B (zh) 2012-04-18
US20080248530A1 (en) 2008-10-09

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