[go: up one dir, main page]

ATE448875T1 - Mikrofluidische vorrichtungen und methoden um temperatur-vermittelte reaktionen durchzuführen - Google Patents

Mikrofluidische vorrichtungen und methoden um temperatur-vermittelte reaktionen durchzuführen

Info

Publication number
ATE448875T1
ATE448875T1 AT01970919T AT01970919T ATE448875T1 AT E448875 T1 ATE448875 T1 AT E448875T1 AT 01970919 T AT01970919 T AT 01970919T AT 01970919 T AT01970919 T AT 01970919T AT E448875 T1 ATE448875 T1 AT E448875T1
Authority
AT
Austria
Prior art keywords
reaction
reaction channel
channel
carrying
methods
Prior art date
Application number
AT01970919T
Other languages
English (en)
Inventor
Seth Stern
Original Assignee
Caliper Life Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Caliper Life Sciences Inc filed Critical Caliper Life Sciences Inc
Application granted granted Critical
Publication of ATE448875T1 publication Critical patent/ATE448875T1/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
AT01970919T 2000-09-14 2001-09-13 Mikrofluidische vorrichtungen und methoden um temperatur-vermittelte reaktionen durchzuführen ATE448875T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23234900P 2000-09-14 2000-09-14
PCT/US2001/028646 WO2002022878A1 (en) 2000-09-14 2001-09-13 Microfluidic devices and methods for performing temperature mediated reactions

Publications (1)

Publication Number Publication Date
ATE448875T1 true ATE448875T1 (de) 2009-12-15

Family

ID=22872752

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01970919T ATE448875T1 (de) 2000-09-14 2001-09-13 Mikrofluidische vorrichtungen und methoden um temperatur-vermittelte reaktionen durchzuführen

Country Status (7)

Country Link
US (2) US6670153B2 (de)
EP (1) EP1317569B1 (de)
JP (1) JP4949600B2 (de)
AT (1) ATE448875T1 (de)
AU (1) AU2001290867A1 (de)
DE (1) DE60140553D1 (de)
WO (1) WO2002022878A1 (de)

Families Citing this family (147)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
US8900811B2 (en) 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US7440684B2 (en) 2001-04-12 2008-10-21 Spaid Michael A Method and apparatus for improved temperature control in microfluidic devices
WO2004011666A2 (en) * 2002-07-26 2004-02-05 Applera Corporation Mg-mediated hot start biochemical reactions
KR100959101B1 (ko) * 2003-02-20 2010-05-25 삼성전자주식회사 Ｐｃｒ 반응기 및 ｐｃｒ 반응기의 입구와 출구의 개폐를조절하는 방법
US7041481B2 (en) * 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
CA2784762A1 (en) 2003-04-10 2004-10-28 President And Fellows Of Harvard College Formation and control of fluidic species
DE10318139B4 (de) * 2003-04-18 2005-02-17 PRO DESIGN Gesellschaft für Produktentwicklung mbH Selbstkonfigurerbarer Biochip
US7038472B1 (en) 2003-05-12 2006-05-02 Caliper Life Sciences, Inc. Methods and systems for measuring internal dimensions of microscale structures
US7731906B2 (en) 2003-07-31 2010-06-08 Handylab, Inc. Processing particle-containing samples
BRPI0414004A (pt) 2003-08-27 2006-10-24 Harvard College controle eletrÈnico de espécies fluìdicas
CA2536360C (en) 2003-08-28 2013-08-06 Celula, Inc. Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network
EP1663497B2 (de) 2003-09-05 2020-03-25 Stokes Bio Limited Mikrofluidanalysesystem
US9597644B2 (en) 2003-09-05 2017-03-21 Stokes Bio Limited Methods for culturing and analyzing cells
DE10353985A1 (de) * 2003-11-19 2005-06-23 Olympus Biosystems Gmbh Einrichtung und Verfahren zum Manipulieren und Analysieren von Mikroobjekten auf Grundlage eines zumindest bereichsweise als fluidisches Mikrosystem oder/und Chipsystem ausgeführten Mikrosystems
JP4592060B2 (ja) * 2004-04-26 2010-12-01 キヤノン株式会社 Ｐｃｒ増幅反応装置、ならびに、該装置を利用するｐｃｒ増幅反応方法
ES2572382T3 (es) 2004-05-03 2016-05-31 Handylab Inc Un dispositivo microfluídico para el procesamiento de muestras que contienen polinucleótidos
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
EP1758981A4 (de) * 2004-05-28 2013-01-16 Wafergen Inc Vorrichtungen und verfahren für multiplex-analysen
DE602005017279D1 (de) 2004-07-28 2009-12-03 Canon Us Life Sciences Inc Verfahren zur überwachung genomischer dna von organismen
US9132398B2 (en) 2007-10-12 2015-09-15 Rheonix, Inc. Integrated microfluidic device and methods
JP4982387B2 (ja) * 2005-02-18 2012-07-25 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッド 微生物のゲノムｄｎａを同定するデバイスおよび方法
DE602006015637D1 (de) * 2005-06-15 2010-09-02 Rhodia Operations Mikrofluidikzirkulationsvorrichtung zur bestimmung von parametern einer physikalischen und/oder chemischen transformation und verwendung davon
DE602005011963D1 (de) * 2005-08-16 2009-02-05 Agilent Technologies Inc Flüssigkeitseinspritzsystem
CN101300352B (zh) * 2005-09-01 2013-03-20 佳能美国生命科学公司 检测、分析和鉴定基因组dna的方法和分子诊断装置
US20090155894A1 (en) * 2005-10-17 2009-06-18 Soper Steven A Electrokinetic Thermal Cycler and Reactor
EP1981624B1 (de) 2006-02-07 2011-09-07 Stokes Bio Limited Flüssigkeitsbrückesystem und Verfahren
JP5254949B2 (ja) 2006-03-15 2013-08-07 マイクロニクス， インコーポレイテッド 一体型の核酸アッセイ
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8088616B2 (en) 2006-03-24 2012-01-03 Handylab, Inc. Heater unit for microfluidic diagnostic system
EP3088083B1 (de) 2006-03-24 2018-08-01 Handylab, Inc. Methode zur durchführung von pcr mittels einer kartusche mit mehreren bahnen
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7846314B2 (en) * 2006-05-11 2010-12-07 Agilent Technologies , Inc Handling a plurality of samples
US7629124B2 (en) * 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
AU2008209561A1 (en) 2007-01-22 2008-07-31 Wafergen, Inc. Apparatus for high throughput chemical reactions
US8691164B2 (en) * 2007-04-20 2014-04-08 Celula, Inc. Cell sorting system and methods
US20080280466A1 (en) * 2007-05-08 2008-11-13 Imation Corp. USB memory device
KR101366487B1 (ko) * 2007-05-18 2014-02-21 메디메이트 홀딩 비.브이. 미세유체 측정 장치
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
JP5651011B2 (ja) 2007-07-13 2015-01-07 ハンディーラブ インコーポレイテッド ポリヌクレオチド捕捉材料およびその使用方法
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8016260B2 (en) 2007-07-19 2011-09-13 Formulatrix, Inc. Metering assembly and method of dispensing fluid
JP5547071B2 (ja) * 2007-08-09 2014-07-09 セルラ・インコーポレイテッド 関連付け多パラメーター単一細胞測定および残留する生物学的材料の回収のための方法および装置
US9114397B2 (en) * 2007-10-25 2015-08-25 Canon U.S. Life Sciences, Inc. Method of reducing cross-contamination in continuous amplification reactions in a channel
US9393566B2 (en) 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
EP2153901A1 (de) * 2008-08-01 2010-02-17 Eppendorf Ag Temperierungsvorrichtung mit Testmöglichkeit und Verfahren zum Testen einer Temperierungsvorrichtung
US8322918B2 (en) * 2008-08-01 2012-12-04 Eppendorf Ag Tempering apparatus with testing device and method for testing a tempering apparatus
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
EP4512526A2 (de) 2008-09-23 2025-02-26 Bio-Rad Laboratories, Inc. Tropfenbasiertes assaysystem
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US8100293B2 (en) 2009-01-23 2012-01-24 Formulatrix, Inc. Microfluidic dispensing assembly
CA2764678C (en) 2009-06-04 2017-12-12 Lockheed Martin Corporation Multiple-sample microfluidic chip for dna analysis
JP6155418B2 (ja) 2009-09-02 2017-07-05 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 多重エマルジョンの合体による、流体を混合するためのシステム
CN102740976B (zh) 2010-01-29 2016-04-20 精密公司 取样-应答微流体盒
JP2013524169A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴によるアッセイ用の検出システム
EP2550528B1 (de) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Tropfenerzeugung für tropfenbasierte prüfungen
CA2814720C (en) 2010-10-15 2016-12-13 Lockheed Martin Corporation Micro fluidic optic design
CN103429331B (zh) 2010-11-01 2016-09-28 伯乐生命医学产品有限公司 用于形成乳液的系统
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
JP2014509865A (ja) 2011-03-18 2014-04-24 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド シグナルの組合せ使用による多重化デジタルアッセイ
ES2617599T3 (es) 2011-04-15 2017-06-19 Becton, Dickinson And Company Termociclador microfluídico de exploración en tiempo real y métodos para termociclado sincronizado y detección óptica de exploración
EP2702175B1 (de) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzungen zur nukleinsäureanalyse
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
KR102121853B1 (ko) 2011-09-30 2020-06-12 벡톤 디킨슨 앤드 컴퍼니 일체화된 시약 스트립
EP2773892B1 (de) 2011-11-04 2020-10-07 Handylab, Inc. Vorrichtung zur vorbereitung von polynukleotidproben
BR112014018995B1 (pt) 2012-02-03 2021-01-19 Becton, Dickson And Company sistemas para executar ensaio automatizado
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
JP5935985B2 (ja) * 2012-03-30 2016-06-15 セイコーエプソン株式会社 熱サイクル装置
JP2013208066A (ja) 2012-03-30 2013-10-10 Seiko Epson Corp 熱サイクル装置及び熱サイクル装置の制御方法
JP5971463B2 (ja) 2012-03-30 2016-08-17 セイコーエプソン株式会社 熱サイクル装置及び熱サイクル装置の制御方法
JP6216494B2 (ja) * 2012-03-30 2017-10-18 セイコーエプソン株式会社 熱サイクル装置及び熱サイクル装置の制御方法
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2881685C (en) 2012-08-14 2023-12-05 10X Genomics, Inc. Microcapsule compositions and methods
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2935559B1 (de) 2012-12-21 2020-09-16 PerkinElmer Health Sciences, Inc. Fluoreszenznachweissystem
US10518262B2 (en) 2012-12-21 2019-12-31 Perkinelmer Health Sciences, Inc. Low elasticity films for microfluidic use
US10065186B2 (en) 2012-12-21 2018-09-04 Micronics, Inc. Fluidic circuits and related manufacturing methods
KR20230003659A (ko) 2013-02-08 2023-01-06 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
US9995412B2 (en) 2013-03-01 2018-06-12 Wave 80 Biosciences, Inc. Long-throw microfluidic actuator
JP2016516562A (ja) * 2013-03-01 2016-06-09 ウェーブ ８０ バイオサイエンシズ インコーポレイテッド マイクロ流体処理のための方法およびシステム
EP2994543B1 (de) 2013-05-07 2018-08-15 Micronics, Inc. Vorrichtung zur herstellung und analyse von nukleinsäuren
EP2994750B1 (de) 2013-05-07 2020-08-12 PerkinElmer Health Sciences, Inc. Mikrofluidische vorrichtungen und verfahren zur durchführung von serumtrennung und blutkreuzproben
CN105189750B (zh) 2013-05-07 2020-07-28 珀金埃尔默健康科学有限公司 使用粘土矿物和碱性溶液制备含核酸样品的方法
JP2015154722A (ja) 2014-02-20 2015-08-27 セイコーエプソン株式会社 核酸増幅反応容器及び核酸増幅反応装置
BR112016023625A2 (pt) 2014-04-10 2018-06-26 10X Genomics, Inc. dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
AU2015279548B2 (en) 2014-06-26 2020-02-27 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
JP2017532042A (ja) 2014-10-29 2017-11-02 １０エックス ゲノミクス，インコーポレイテッド 標的化核酸配列決定のための方法及び組成物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
BR112017014902A2 (pt) 2015-01-12 2018-03-13 10X Genomics Inc processos e sistemas para a preparação de bibliotecas de sequenciamento de ácido nucleico e bibliotecas preparadas usando os mesmos
JP6620160B2 (ja) 2015-02-20 2019-12-11 タカラ バイオ ユーエスエー， インコーポレイテッド 単一細胞の迅速かつ正確な分注、視覚化及び解析のための方法
JP2018505688A (ja) 2015-02-24 2018-03-01 １０エックス ゲノミクス，インコーポレイテッド 標的化核酸配列包括度（ｃｏｖｅｒａｇｅ）のための方法
EP3262407B1 (de) 2015-02-24 2023-08-30 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
ES2926495T3 (es) 2015-12-04 2022-10-26 10X Genomics Inc Métodos y composiciones para el análisis de ácidos nucleicos
JP6206688B2 (ja) * 2016-05-11 2017-10-04 セイコーエプソン株式会社 熱サイクル装置
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
ES2987492T3 (es) 2016-06-27 2024-11-15 Zoetis Services Llc Dispositivo con conductos modificados
JP7075394B2 (ja) 2016-07-21 2022-05-25 タカラ バイオ ユーエスエー， インコーポレイテッド マルチウェルデバイスを用いたマルチｚ撮像及び分注
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN110730690A (zh) * 2017-04-21 2020-01-24 爱贝斯股份有限公司 用于微流体分析的系统、装置和方法
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3445876B1 (de) 2017-05-26 2023-07-05 10X Genomics, Inc. Einzelzellenanalyse von transposasezugänglichem chromatin
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3775271B1 (de) 2018-04-06 2025-03-12 10X Genomics, Inc. Systeme und verfahren zur qualitätskontrolle in einer einzelzellenverarbeitung

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8917963D0 (en) * 1989-08-05 1989-09-20 Scras Apparatus for repeated automatic execution of a thermal cycle for treatment of biological samples
US5639423A (en) 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US6001229A (en) 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5863502A (en) 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
HUP9902418A3 (en) 1996-05-09 2001-10-29 Dimensional Pharm Inc Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
US5779868A (en) 1996-06-28 1998-07-14 Caliper Technologies Corporation Electropipettor and compensation means for electrophoretic bias
US6429025B1 (en) * 1996-06-28 2002-08-06 Caliper Technologies Corp. High-throughput screening assay systems in microscale fluidic devices
US5800690A (en) 1996-07-03 1998-09-01 Caliper Technologies Corporation Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces
WO1998045481A1 (en) * 1997-04-04 1998-10-15 Caliper Technologies Corporation Closed-loop biochemical analyzers
US5965410A (en) 1997-09-02 1999-10-12 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
EP1009995A4 (de) * 1997-09-02 2007-05-02 Caliper Life Sciences Inc Mikrofluidisches system mit elektrofluidischer und elektrothermischer regelung
US6103537A (en) * 1997-10-02 2000-08-15 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
US6202141B1 (en) 1998-06-16 2001-03-13 International Business Machines Corporation Method and apparatus for performing vector operation using separate multiplication on odd and even data elements of source vectors
US6062261A (en) * 1998-12-16 2000-05-16 Lockheed Martin Energy Research Corporation MicrofluIdic circuit designs for performing electrokinetic manipulations that reduce the number of voltage sources and fluid reservoirs
US6150119A (en) * 1999-01-19 2000-11-21 Caliper Technologies Corp. Optimized high-throughput analytical system
US6300075B1 (en) 1999-02-03 2001-10-09 Ortho-Clinical Diagnostics, Inc Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6221600B1 (en) * 1999-10-08 2001-04-24 Board Of Regents, The University Of Texas System Combinatorial oligonucleotide PCR: a method for rapid, global expression analysis

Also Published As

Publication number Publication date
WO2002022878A1 (en) 2002-03-21
JP2004508837A (ja) 2004-03-25
EP1317569A1 (de) 2003-06-11
US6670153B2 (en) 2003-12-30
US20040081997A1 (en) 2004-04-29
EP1317569B1 (de) 2009-11-18
US20020119536A1 (en) 2002-08-29
AU2001290867A1 (en) 2002-03-26
EP1317569A4 (de) 2004-06-30
JP4949600B2 (ja) 2012-06-13
DE60140553D1 (de) 2009-12-31
US7153673B2 (en) 2006-12-26

Similar Documents

Publication Publication Date Title
DE60140553D1 (de) MIKROFLUIDISCHE VORRICHTUNGEN UND METHODEN UM TEMPERATUR-VERMITTELTE REAKTIONEN DURCHZUFüHREN
DE60226500D1 (en) Late-pcr
ES2174884T3 (es) Amplificacion de cadenas por desplazamiento que utiliza encimas termofilicas.
DE602004022253D1 (de) Verfahren zur amplifikation und sequenzierung von nukleinsäuren
WO2002052030A3 (en) Compositions and methods enabling a totally internally controlled amplification reaction
ATE382095T1 (de) In-vitro verfahren zur bereitstellung von fragmenten für die dns sequenzierung
BR9808368A (pt) Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse.
ATE148925T1 (de) Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion
WO2003035811A8 (en) Remediation of a hydrocarbon containing formation
DE60116616D1 (de) Vorrichtung und verfahren zur behandlung von erdöllagerstätten
HK1018080A1 (en) Process for direct sequencing during template amplification
DE602006018472D1 (de) Analyse von boten-rna und mikro-rna im gleichen reaktionsansatz
DE69713866D1 (de) Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen und seine Anwendung
TW200520100A (en) Selective heating using flash anneal
ATE481503T1 (de) Verfahren zur genotypisierung von antigenen von blutzellen und ein kit zur genotypisierung
TW430866B (en) Thermal treatment apparatus
HK1042324A1 (en) Method for controlling the extension of an oligonucleotide
ATE466283T1 (de) Zentrifugalvorrichtung und verfahren zur durchführung von bindungsversuchen
DE50213489D1 (de) Verfahren zum nachweis von cytosin-methylierungen in immobilisierten dna proben
DE50112799D1 (de) Verfahren zum nachweis von cytosin-methylierungen
NO20043949L (no) Fremgangsmate for fremstilling av salpetersyre
WO2001075139A8 (en) Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization
BRPI0504146A (pt) processo catalìtico seletivo regenerativo
Gibson-Daw et al. Rapid microfluidic analysis of a Y-STR multiplex for screening of forensic samples
DE60014889D1 (de) Verfahren und vorrichtung zum verschweissen von länglichen elementen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties