[go: up one dir, main page]

ATE399880T1 - Ein in-vitro-verfahren zur herstellung einer dna- bibliothek - Google Patents

Ein in-vitro-verfahren zur herstellung einer dna- bibliothek

Info

Publication number
ATE399880T1
ATE399880T1 AT98907911T AT98907911T ATE399880T1 AT E399880 T1 ATE399880 T1 AT E399880T1 AT 98907911 T AT98907911 T AT 98907911T AT 98907911 T AT98907911 T AT 98907911T AT E399880 T1 ATE399880 T1 AT E399880T1
Authority
AT
Austria
Prior art keywords
primers
templates
extended
polynucleotides
induced
Prior art date
Application number
AT98907911T
Other languages
English (en)
Inventor
Jesper Vind
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE399880T1 publication Critical patent/ATE399880T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
AT98907911T 1997-03-18 1998-03-18 Ein in-vitro-verfahren zur herstellung einer dna- bibliothek ATE399880T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK30797 1997-03-18
DK43497 1997-04-17
DK62597 1997-05-30

Publications (1)

Publication Number Publication Date
ATE399880T1 true ATE399880T1 (de) 2008-07-15

Family

ID=27220576

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98907911T ATE399880T1 (de) 1997-03-18 1998-03-18 Ein in-vitro-verfahren zur herstellung einer dna- bibliothek

Country Status (14)

Country Link
EP (1) EP0973940B1 (de)
JP (1) JP2001515356A (de)
KR (1) KR20000076363A (de)
CN (1) CN1249784A (de)
AT (1) ATE399880T1 (de)
AU (1) AU741834B2 (de)
BR (1) BR9808368A (de)
CA (1) CA2284253A1 (de)
DE (1) DE69839668D1 (de)
DK (1) DK0973940T3 (de)
IL (1) IL131966A0 (de)
NZ (1) NZ337900A (de)
PL (1) PL335860A1 (de)
WO (1) WO1998041653A1 (de)

Families Citing this family (130)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
AU746786B2 (en) 1997-12-08 2002-05-02 California Institute Of Technology Method for creating polynucleotide and polypeptide sequences
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
IL140100A0 (en) * 1998-06-29 2002-02-10 Phylos Inc Methods of generating highly diverse libraries
EP1104459A1 (de) 1998-08-12 2001-06-06 Maxygen, Inc. Dna-shuffling von monooxygenase genen für die herstellung von industriellen chemikalien
WO2000020573A2 (en) 1998-10-07 2000-04-13 Maxygen, Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
ATE332968T1 (de) 1998-10-26 2006-08-15 Novozymes As Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
WO2000028018A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes
DE69941364D1 (de) * 1998-11-27 2009-10-15 Novozymes As Varianten eines lipolytischen enzyms
US7312062B2 (en) 1998-11-27 2007-12-25 Novozymes A/S Lipolytic enzyme variants
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
EP2253704B1 (de) 1999-01-19 2015-08-19 Codexis Mayflower Holdings, LLC Durch Oligonukleotide-vermittelte Nukleinsäuren-rekombination
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
KR20010102069A (ko) 1999-02-11 2001-11-15 추후제출 고효율 질량 분석
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
IL146743A0 (en) * 1999-05-31 2002-07-25 Novozymes As Screening method for peptides
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
EP1276900A2 (de) 2000-01-11 2003-01-22 Maxygen, Inc. Integrierte systeme und verfahren zur generierung und screening der diversität
US6248541B1 (en) 2000-04-21 2001-06-19 Genencor International, Inc. Screening under nutrient limited conditions
US6534292B1 (en) 2000-05-08 2003-03-18 Genencor International, Inc. Methods for forming recombined nucleic acids
US7115403B1 (en) 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
US7094875B2 (en) 2000-06-23 2006-08-22 Maxygen, Inc. Co-stimulatory polypeptides
WO2002000897A2 (en) 2000-06-23 2002-01-03 Maxygen, Inc. Novel chimeric promoters
US6994963B1 (en) 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
AUPQ974900A0 (en) * 2000-08-29 2000-09-21 Macquarie Research Limited Degenerate oligonucleotide gene-shuffling
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
US6783941B2 (en) 2000-12-06 2004-08-31 Novozymes A/S Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes
WO2002046396A1 (en) * 2000-12-06 2002-06-13 Novozymes A/S Method for producing a polynucleotide library
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
WO2002083868A2 (en) 2001-04-16 2002-10-24 California Institute Of Technology Peroxide-driven cytochrome p450 oxygenase variants
US7226768B2 (en) 2001-07-20 2007-06-05 The California Institute Of Technology Cytochrome P450 oxygenases
ES2564570T3 (es) 2002-03-01 2016-03-23 Codexis Mayflower Holdings, Llc Métodos, sistemas y software para la identificación de biomoléculas funcionales
EP1488335A4 (de) 2002-03-09 2006-11-15 Maxygen Inc Optimierung von crossover-punkten für die gerichtete evolution
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
CA2481411C (en) 2002-04-19 2016-06-14 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
DK1504098T4 (da) 2002-05-17 2011-05-23 Alligator Bioscience Ab Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
EP1576151A4 (de) 2002-08-06 2006-05-17 Verdia Inc Ap1-aminoxidase-varianten
WO2004016811A2 (en) * 2002-08-19 2004-02-26 Danmarks Tekniske Universitet (Dtu) Methods and kit for genes shuffling using tagged primers
EP3508578A1 (de) 2003-03-06 2019-07-10 BASF Enzymes, LLC Amylasen, nukleinsäuren zur codierung davon und verfahren zur herstellung und verwendung davon
EP2853593B1 (de) 2003-03-07 2017-10-04 DSM IP Assets B.V. Hydrolasen, Nucleinsäuren zur Codierung davon sowie Verfahren zur Herstellung und Verwendung davon
ES2598037T3 (es) 2003-04-04 2017-01-24 Basf Enzymes Llc Pectato liasas, ácidos nucleicos que las codifican y métodos para su preparación y uso
CA2521284C (en) 2003-04-29 2014-07-08 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
WO2005017105A2 (en) 2003-06-17 2005-02-24 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
DK1641910T3 (da) 2003-07-02 2013-05-21 Verenium Corp Glucanaser, nucleinsyrer, som koder for disse, og fremgangsmåder til at fremstille og benytte disse
WO2005021714A2 (en) 2003-08-11 2005-03-10 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
WO2005017116A2 (en) 2003-08-11 2005-02-24 California Institute Of Technology Thermostable peroxide-driven cytochrome p450 oxygenase variants and methods of use
DE602005023727D1 (de) 2004-06-09 2010-11-04 Pioneer Hi Bred Int Plastiden transit peptide
CN103173282A (zh) 2004-06-16 2013-06-26 帝斯曼知识产权资产管理有限公司 对叶绿素进行酶促脱色的组合物和方法
CA2614769A1 (en) 2005-03-10 2006-09-21 Verenium Corporation Lyase enzymes, nucleic acids encoding them and methods for making and using them
NZ583532A (en) 2005-03-15 2011-09-30 Verenium Corp Cellulases, nucleic acids encoding them and methods for making and using them
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
US11214817B2 (en) 2005-03-28 2022-01-04 California Institute Of Technology Alkane oxidation by modified hydroxylases
CN100366752C (zh) * 2005-12-28 2008-02-06 河北省农林科学院遗传生理研究所 一种利用原子变构衍生dna序列多样性的方法
EP1987142A4 (de) 2006-02-02 2009-07-15 Verenium Corp Esterasen und damit zusammenhängende nukleinsäuren und verfahren
NZ595499A (en) 2006-02-10 2013-05-31 Verenium Corp Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
EP3406621A1 (de) 2006-02-14 2018-11-28 BP Corporation North America Inc. Xylanasen, dafür codierende nukleinsäuren sowie verfahren zur herstellung und verwendung davon
ATE548450T1 (de) 2006-03-07 2012-03-15 Verenium Corp Aldolasen, nukleinsäuren zu ihrer kodierung sowie verfahren zu ihrer herstellung und verwendung
BRPI0708673A2 (pt) 2006-03-07 2011-06-07 Cargill Inc método para fabricação de polipeptìdeos com atividade de aldolase e polinucleotìdeos que codificam estes polipeptìdeos
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8026085B2 (en) 2006-08-04 2011-09-27 California Institute Of Technology Methods and systems for selective fluorination of organic molecules
EP2069389B1 (de) 2006-08-04 2014-10-08 BP Corporation North America Inc. Glucanasen, nukleinsäuren zu ihrer kodierung sowie verfahren zu ihrer herstellung und verwendung
ES2451266T3 (es) 2006-09-21 2014-03-26 Dsm Ip Assets B.V. Fosfolipasas, ácidos nucleicos que las codifican, y métodos para obtenerlas y usarlas
DK2617814T3 (da) 2006-09-21 2016-02-22 Basf Enzymes Llc Phytaser, nukleinsyrer, der koder for disse, og fremgangsmåder til frembringelse og anvendelse heraf
DK2479267T3 (en) 2006-12-21 2017-03-27 Basf Enzymes Llc Amylases and Glucoamylases, Nucleic Acids Encoding Them, and Methods for Preparing and Using Them
MX2009008129A (es) 2007-01-30 2010-03-17 Verenium Corp Enzimas para el tratamiento de materiales ligno-celulosicos, acidos nucleicos que las codifican y metodos para hacerlas y usarlas.
CN101952437B (zh) 2007-10-03 2014-04-09 维莱尼姆公司 木聚糖酶、编码它们的核酸以及其制备和应用方法
CA2710683A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
US8541220B2 (en) 2008-01-03 2013-09-24 Verenium Corporation Isomerases, nucleic acids encoding them and methods for making and using them
EP2297310A2 (de) 2008-05-23 2011-03-23 E. I. du Pont de Nemours and Company Neue dgat-gene für gesteigerte samenspeicherlipidproduktion und veränderte fettsäureprofile bei ölsamenpflanzen
KR20110044753A (ko) * 2008-08-08 2011-04-29 토소가부시키가이샤 기능 개선된 ｒｎａ 중합효소 변이체
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
CN102227503B (zh) 2008-09-26 2015-10-21 托卡根公司 重组载体
CA2763283C (en) 2009-05-21 2021-01-12 Verenium Corporation Phytases, nucleic acids encoding them and methods for making and using them
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
WO2011127405A1 (en) 2010-04-08 2011-10-13 Sanford-Burnham Medical Research Institute Methods and compositions for enhanced delivery of compounds
US9187762B2 (en) 2010-08-13 2015-11-17 Pioneer Hi-Bred International, Inc. Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity
CN102560688B (zh) * 2010-12-15 2013-11-20 深圳华大基因科技服务有限公司 一种新的基于illumina测序平台的文库构建方法
WO2013016207A2 (en) 2011-07-22 2013-01-31 California Institute Of Technology Stable fungal cel6 enzyme variants
CN104411823A (zh) 2012-05-04 2015-03-11 纳幕尔杜邦公司 包含具有大范围核酸酶活性的序列的组合物和方法
EP3450549B1 (de) 2012-10-29 2020-09-30 University Of Rochester Artemisininderivate, verfahren zu deren herstellung und deren verwendung als antimalariamittel
US20140287419A1 (en) 2013-03-14 2014-09-25 Arzeda Corp. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
EP2970935A1 (de) 2013-03-14 2016-01-20 Pioneer Hi-Bred International, Inc. Zusammensetzungen mit dicamba-decarboxylaseaktivität und verfahren zur verwendung
EP2971000A4 (de) 2013-03-15 2016-11-23 Pioneer Hi Bred Int Phi-4-polypeptide und verfahren zu deren verwendung
WO2015023846A2 (en) 2013-08-16 2015-02-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP4159028A1 (de) 2013-09-13 2023-04-05 Pioneer Hi-Bred International, Inc. Insektizidproteine und verfahren zu deren verwendung
CN103539744B (zh) * 2013-10-17 2016-03-23 北京颖泰嘉和生物科技股份有限公司 一种制备3-(3,5-二氯苯基)-2,4-咪唑烷二酮的方法
EP4122945A1 (de) 2013-12-23 2023-01-25 University of Rochester Verfahren und zusammensetzungen zur ribosomalen synthese von makrocyclischen peptiden
US10480007B2 (en) 2014-02-07 2019-11-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants
CN117903266A (zh) 2014-02-07 2024-04-19 先锋国际良种公司 杀昆虫蛋白及其使用方法
MX2017004745A (es) 2014-10-16 2017-10-20 Pioner Hi-Bred Int Inc Proteinas insecticidas y metodos de uso.
WO2016114973A1 (en) 2015-01-15 2016-07-21 Pioneer Hi Bred International, Inc Insecticidal proteins and methods for their use
WO2016134521A1 (en) * 2015-02-27 2016-09-01 The University Of Hong Kong Dna display and methods thereof
BR112017019419B1 (pt) 2015-03-11 2022-10-11 E. I. Du Pont De Nemours And Company Construto de dna, método de obtenção de uma planta transgênica e método para controlar uma população de praga de verme da raiz do milho
CN108064233B (zh) 2015-05-19 2022-07-15 先锋国际良种公司 杀昆虫蛋白及其使用方法
UA124495C2 (uk) 2015-08-06 2021-09-29 Піонір Хай-Бред Інтернешнл, Інк. Інсектицидний білок рослинного походження та спосіб його застосування
EP3390431A1 (de) 2015-12-18 2018-10-24 Pioneer Hi-Bred International, Inc. Insektizide proteine und verfahren zu deren verwendung
EA201892293A1 (ru) 2016-05-04 2019-04-30 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
CN109788735A (zh) 2016-07-01 2019-05-21 先锋国际良种公司 来自植物的杀昆虫蛋白及其使用方法
CA3038806A1 (en) 2016-11-01 2018-05-11 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11174295B2 (en) 2016-12-14 2021-11-16 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11213028B2 (en) 2016-12-22 2022-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US20190390219A1 (en) 2017-02-08 2019-12-26 Pioneer Hi-Bred International, Inc. Insecticidal combinations of plant derived insecticidal proteins and methods for their use
WO2018208882A1 (en) 2017-05-11 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11634400B2 (en) 2017-08-19 2023-04-25 University Of Rochester Micheliolide derivatives, methods for their preparation and their use as anticancer and antiinflammatory agents
AU2019234566B2 (en) 2018-03-14 2024-09-26 Hexima Limited Insecticidal proteins from plants and methods for their use
CA3092075A1 (en) 2018-03-14 2019-09-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
CN108588050B (zh) * 2018-05-14 2021-06-25 北京艾克伦医疗科技有限公司 Dna聚合酶以及核酸检测方法和试剂盒
BR112022013503A2 (pt) 2020-01-10 2022-09-13 Univ California Plataforma biossintética para a produção de ácido olivetólico e análogos de ácido olivetólico
BR112022027035A2 (pt) 2020-07-14 2023-04-11 Pioneer Hi Bred Int Proteínas inseticidas e métodos para uso das mesmas
CN114197061A (zh) * 2022-02-17 2022-03-18 北京贝瑞和康生物技术有限公司 用于构建检测染色体拷贝数变异的测序文库的方法和试剂盒
EP4490202A1 (de) 2022-03-11 2025-01-15 University of Rochester Cyclopeptikörper und verwendungen davon

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5629179A (en) * 1995-03-17 1997-05-13 Novagen, Inc. Method and kit for making CDNA library
DK0843725T3 (da) 1995-08-11 2002-08-12 Novozymes As Fremgangmåde til fremstilling af polypeptidvarianter
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis

Also Published As

Publication number Publication date
CA2284253A1 (en) 1998-09-24
IL131966A0 (en) 2001-03-19
JP2001515356A (ja) 2001-09-18
DK0973940T3 (da) 2008-10-20
CN1249784A (zh) 2000-04-05
PL335860A1 (en) 2000-05-22
AU6611598A (en) 1998-10-12
NZ337900A (en) 2001-05-25
WO1998041653A1 (en) 1998-09-24
KR20000076363A (ko) 2000-12-26
EP0973940A1 (de) 2000-01-26
AU741834B2 (en) 2001-12-13
DE69839668D1 (de) 2008-08-14
BR9808368A (pt) 2000-05-23
EP0973940B1 (de) 2008-07-02

Similar Documents

Publication Publication Date Title
ATE399880T1 (de) Ein in-vitro-verfahren zur herstellung einer dna- bibliothek
NO20021223L (no) Fremgangsmater og sammensetninger for lineaer, isoterm amplifisering av polynukleotidsekvenser
ATE257861T1 (de) Methoden und zusammensetzungen zur effizienten nukleinsaeuresequenzierung
DK0408295T3 (da) Fremgangsmåder til opformering af nukleinsyresekvenser
DE69820111D1 (de) Massenspektroskopische verfahren zur sequenzierung von nukleinsäuren
WO2002072773A3 (en) Methods and compositions for amplification of rna sequences
DE60034396D1 (de) Reaktionsgemisch und Verfahren für RT-PCR mittels einer homopolymeren Nukleinsäure
DE60220025D1 (de) Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
DE69307503D1 (de) Chemisches verfahren zur analyse von dns-sequenzen
ATE542917T1 (de) Zusammensetzungen, verfahren und kits zur bestimmung des vorhandenseins von trichomonas vaginalis in einer testprobe
ATE361377T1 (de) Leistungstarke verknüpfung von nukleinsäuresegmenten
ATE440147T1 (de) Erhíhung der spezifität von nukleinsäureamplifizierungen durch trägernukleinsäuren
WO2005118608A3 (en) 2’-nitrobenzyl-modified ribonucleotides
EP1103624A4 (de) Verbessertes verfahren zur amplifikation von nukleinsäuren
DE60123481D1 (de) Verfahren zur durchführung einer subtraktiven hybridisierung
DE60125054D1 (de) Verfahren zur amplifikation von nukleinsäuren, das mittel zur strangtrennung und universelle sequenzmarker bereitstellt
EP1634959A3 (de) Methode zur Bereitstellung von Proben und Mikroarrays, deren Verwendung und ein Computerprogramm für die Herstellung der Proben
WO2004099443A3 (en) Method for selection of optimal microarray probes
HUP0000500A2 (hu) In vitro eljárás DNS-könyvtár készítésére
WO2024076689A3 (en) Size-discriminating nucleic acid-binding complexes and methods of use thereof
WO2004016755A3 (en) Amplification of target nucleotide sequence without polymerase chain reaction
WO2002050094A3 (en) Nucleic acid synthesis by primer extension on successive templates
ATE122095T1 (de) Verfahren zur herstellung von chimären antikörpern.
GB0216026D0 (en) Nucleic acid amplification method
KR20130128129A (ko) 검출율이 향상된 수동식 중합효소 연쇄 반응 방법

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties