ATE397619T1 - Verfahren zur proteinisolierung unteranoxischen bedingungen - Google Patents
Verfahren zur proteinisolierung unteranoxischen bedingungenInfo
- Publication number
- ATE397619T1 ATE397619T1 AT04706187T AT04706187T ATE397619T1 AT E397619 T1 ATE397619 T1 AT E397619T1 AT 04706187 T AT04706187 T AT 04706187T AT 04706187 T AT04706187 T AT 04706187T AT E397619 T1 ATE397619 T1 AT E397619T1
- Authority
- AT
- Austria
- Prior art keywords
- protein isolation
- anoxic conditions
- under anoxic
- isolation under
- present
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 238000000164 protein isolation Methods 0.000 title abstract 2
- 238000002955 isolation Methods 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108010076181 Proinsulin Proteins 0.000 abstract 1
- 239000003638 chemical reducing agent Substances 0.000 abstract 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP03100208 | 2003-01-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE397619T1 true ATE397619T1 (de) | 2008-06-15 |
Family
ID=32799004
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04706187T ATE397619T1 (de) | 2003-01-31 | 2004-01-29 | Verfahren zur proteinisolierung unteranoxischen bedingungen |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US20060128942A1 (de) |
| EP (1) | EP1592703B1 (de) |
| JP (1) | JP4992029B2 (de) |
| CN (1) | CN100395263C (de) |
| AT (1) | ATE397619T1 (de) |
| CA (1) | CA2514416C (de) |
| DE (1) | DE602004014251D1 (de) |
| ES (1) | ES2306981T3 (de) |
| PL (1) | PL212317B1 (de) |
| WO (1) | WO2004067556A1 (de) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100395263C (zh) * | 2003-01-31 | 2008-06-18 | 欧加农股份有限公司 | 缺氧条件下的蛋白质分离方法 |
| US20120214199A1 (en) * | 2011-02-23 | 2012-08-23 | Elona Biotechnologies | Lis-pro proinsulin compositions and methods of producing lis-pro insulin analogs therefrom |
| EP4335863A3 (de) | 2007-07-09 | 2024-07-24 | Genentech, Inc. | Verhinderung der disulfidbindungsreduktion während der rekombinanten herstellung von polypeptiden |
| CN101782549A (zh) * | 2010-03-29 | 2010-07-21 | 内蒙古蒙牛乳业（集团）股份有限公司 | 检测牛乳制品中酪蛋白磷酸肽含量的方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ199391A (en) | 1981-01-02 | 1985-12-13 | Genentech Inc | Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin |
| EP0196056B1 (de) | 1985-03-28 | 1991-05-22 | Chiron Corporation | Expression durch Verwendung von Fusionsgenen für Proteinproduktion |
| US4801691A (en) * | 1987-05-15 | 1989-01-31 | International Minerals & Chemical Corp. | Method for removing sodium dodecyl sulfate from sodium dodecyl sulfate solubilized protein solutions |
| US5593860A (en) * | 1987-08-14 | 1997-01-14 | Bio-Technology General Corp. | Expression plasmids containing the deo promoter, and bacterial hosts containing the plasmids |
| US6875589B1 (en) * | 1988-06-23 | 2005-04-05 | Hoechst Aktiengesellschaft | Mini-proinsulin, its preparation and use |
| EP0347781B1 (de) * | 1988-06-23 | 1994-02-16 | Hoechst Aktiengesellschaft | Mini-Proinsulin, seine Herstellung und Verwendung |
| US4923967A (en) * | 1988-09-26 | 1990-05-08 | Eli Lilly And Company | Purification and refolding of recombinant proteins |
| DE3901718A1 (de) | 1989-01-21 | 1990-07-26 | Hoechst Ag | Verfahren zur renaturierung falscher rekombinanten von insulinvorlaeufern |
| DE69001248T2 (de) * | 1989-05-04 | 1993-10-14 | Genentech Inc | Verfahren und zusammensetzungen zur isolation von menschlichem relaxin. |
| US6020145A (en) * | 1989-06-30 | 2000-02-01 | Bristol-Myers Squibb Company | Methods for determining the presence of carcinoma using the antigen binding region of monoclonal antibody BR96 |
| US5840851A (en) * | 1993-07-23 | 1998-11-24 | Plomer; J. Jeffrey | Purification of hemoglobin |
| US6001604A (en) * | 1993-12-29 | 1999-12-14 | Bio-Technology General Corp. | Refolding of proinsulins without addition of reducing agents |
| SG71728A1 (en) * | 1994-07-29 | 2000-04-18 | Innogenetics Nv | Purified hepatitis c virus envelope proteins for diagnostic and therapeutic use |
| DK0871474T4 (da) | 1994-12-29 | 2012-01-09 | Ferring Int Ct Sa | Fremstilling af human insulin |
| KR100253916B1 (ko) * | 1997-12-29 | 2000-05-01 | 김충환 | 사람 인슐린 전구체의 제조방법 |
| CN100395263C (zh) * | 2003-01-31 | 2008-06-18 | 欧加农股份有限公司 | 缺氧条件下的蛋白质分离方法 |
| UA96139C2 (uk) * | 2005-11-08 | 2011-10-10 | Дженентек, Інк. | Антитіло до нейропіліну-1 (nrp1) |
-
2004
- 2004-01-29 CN CNB2004800030976A patent/CN100395263C/zh not_active Expired - Lifetime
- 2004-01-29 JP JP2006501710A patent/JP4992029B2/ja not_active Expired - Lifetime
- 2004-01-29 CA CA2514416A patent/CA2514416C/en not_active Expired - Lifetime
- 2004-01-29 WO PCT/EP2004/000953 patent/WO2004067556A1/en active IP Right Grant
- 2004-01-29 PL PL377927A patent/PL212317B1/pl unknown
- 2004-01-29 ES ES04706187T patent/ES2306981T3/es not_active Expired - Lifetime
- 2004-01-29 EP EP04706187A patent/EP1592703B1/de not_active Expired - Lifetime
- 2004-01-29 DE DE602004014251T patent/DE602004014251D1/de not_active Expired - Lifetime
- 2004-01-29 US US10/543,524 patent/US20060128942A1/en not_active Abandoned
- 2004-01-29 AT AT04706187T patent/ATE397619T1/de not_active IP Right Cessation
-
2012
- 2012-05-09 US US13/467,305 patent/US8802395B2/en not_active Expired - Lifetime
-
2014
- 2014-07-08 US US14/325,822 patent/US9505802B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DE602004014251D1 (de) | 2008-07-17 |
| US20140323687A1 (en) | 2014-10-30 |
| US20130150553A1 (en) | 2013-06-13 |
| US8802395B2 (en) | 2014-08-12 |
| JP2007506406A (ja) | 2007-03-22 |
| CA2514416A1 (en) | 2004-08-12 |
| PL377927A1 (pl) | 2006-02-20 |
| WO2004067556A1 (en) | 2004-08-12 |
| HK1078885A1 (en) | 2006-03-24 |
| CN1745092A (zh) | 2006-03-08 |
| EP1592703B1 (de) | 2008-06-04 |
| ES2306981T3 (es) | 2008-11-16 |
| PL212317B1 (pl) | 2012-09-28 |
| EP1592703A1 (de) | 2005-11-09 |
| US9505802B2 (en) | 2016-11-29 |
| JP4992029B2 (ja) | 2012-08-08 |
| US20060128942A1 (en) | 2006-06-15 |
| CN100395263C (zh) | 2008-06-18 |
| CA2514416C (en) | 2012-08-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |