ATE397081T1 - Erhöhte expression von proteinen mittels gfp - Google Patents
Erhöhte expression von proteinen mittels gfpInfo
- Publication number
- ATE397081T1 ATE397081T1 AT00946940T AT00946940T ATE397081T1 AT E397081 T1 ATE397081 T1 AT E397081T1 AT 00946940 T AT00946940 T AT 00946940T AT 00946940 T AT00946940 T AT 00946940T AT E397081 T1 ATE397081 T1 AT E397081T1
- Authority
- AT
- Austria
- Prior art keywords
- proteins
- gfp
- increased expression
- constructs
- amino acids
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 abstract 1
- 102000004144 Green Fluorescent Proteins Human genes 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 230000004927 fusion Effects 0.000 abstract 1
- 239000005090 green fluorescent protein Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
- C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US35112499A | 1999-07-10 | 1999-07-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE397081T1 true ATE397081T1 (de) | 2008-06-15 |
Family
ID=23379676
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00946940T ATE397081T1 (de) | 1999-07-10 | 2000-06-26 | Erhöhte expression von proteinen mittels gfp |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1194578B1 (de) |
| AT (1) | ATE397081T1 (de) |
| AU (1) | AU6062300A (de) |
| CA (1) | CA2378549A1 (de) |
| DE (1) | DE60039049D1 (de) |
| WO (1) | WO2001004331A2 (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7300775B2 (en) | 1999-12-29 | 2007-11-27 | Verenium Corporation | Methods for producing α-substituted carboxylic acids using nitrilases and strecker reagents |
| US7521216B2 (en) | 1999-12-29 | 2009-04-21 | Verenium Corporation | Nitrilases and methods for making and using them |
| US7608445B1 (en) | 1999-12-29 | 2009-10-27 | Verenium Corporation | Nitrilases, nucleic acids encoding them and methods for making and using them |
| WO2002063029A1 (en) * | 2001-02-02 | 2002-08-15 | Iconix Pharmaceuticals, Inc. | Alteration of phenotype due to heterologous genes |
| AU2003251523A1 (en) | 2002-06-13 | 2003-12-31 | Diversa Corporation | Processes for making (r)-ethyl 4-cyano-3-hydroxybutyric acid |
| DK2115153T3 (da) | 2007-03-01 | 2013-09-08 | Bp Corp North America Inc | Nitrilaser, nucleinsyrer som koder for dem og fremgangsmåder til at fremstille og anvende disse |
| ES2336754A1 (es) * | 2008-07-31 | 2010-04-15 | Plant Bioproducts, S.L | Metodo para mejorar la expresion de proteinas en cloroplastos. |
| AR074941A1 (es) | 2009-01-07 | 2011-02-23 | Bayer Cropscience Sa | Plantas transplastomicas exentas de marcador de seleccion |
| CN104450775B (zh) * | 2014-12-04 | 2018-04-27 | 中国农业科学院作物科学研究所 | 抗草甘膦转基因大豆及其制备方法与应用 |
| US9920102B2 (en) | 2015-05-15 | 2018-03-20 | Albert Einstein College Of Medicine, Inc. | Fusion tags for protein expression |
| CN108085325B (zh) * | 2018-01-31 | 2020-07-03 | 华中农业大学 | 一种抗草甘膦基因以及转基因抗草甘膦烟草的培育方法 |
| CN112321724B (zh) * | 2020-12-04 | 2021-08-10 | 中国农业科学院农业质量标准与检测技术研究所 | 一种基于epsp合酶与gfp融合蛋白检测草甘膦的快速检测方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5693507A (en) * | 1988-09-26 | 1997-12-02 | Auburn University | Genetic engineering of plant chloroplasts |
| US5545817A (en) * | 1994-03-11 | 1996-08-13 | Calgene, Inc. | Enhanced expression in a plant plastid |
| GB9504446D0 (en) * | 1995-03-06 | 1995-04-26 | Medical Res Council | Improvements in or relating to gene expression |
| ES2242234T3 (es) * | 1996-09-12 | 2005-11-01 | Syngenta Participations Ag | Plantas transgenicas que expresan enzimas celuloliticas. |
| JP2002520024A (ja) * | 1998-07-10 | 2002-07-09 | カルジーン エルエルシー | 植物色素体における除草剤耐性遺伝子の発現 |
-
2000
- 2000-06-26 DE DE60039049T patent/DE60039049D1/de not_active Expired - Lifetime
- 2000-06-26 AT AT00946940T patent/ATE397081T1/de not_active IP Right Cessation
- 2000-06-26 WO PCT/US2000/018096 patent/WO2001004331A2/en active Search and Examination
- 2000-06-26 AU AU60623/00A patent/AU6062300A/en not_active Abandoned
- 2000-06-26 CA CA002378549A patent/CA2378549A1/en not_active Abandoned
- 2000-06-26 EP EP00946940A patent/EP1194578B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE60039049D1 (de) | 2008-07-10 |
| WO2001004331A3 (en) | 2001-07-19 |
| EP1194578A2 (de) | 2002-04-10 |
| EP1194578B1 (de) | 2008-05-28 |
| CA2378549A1 (en) | 2001-01-18 |
| AU6062300A (en) | 2001-01-30 |
| WO2001004331A2 (en) | 2001-01-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |