ATE212367T1 - Asymmetrische cyaninfarbstoffe als fluoreszenzlöscher - Google Patents
Asymmetrische cyaninfarbstoffe als fluoreszenzlöscherInfo
- Publication number
- ATE212367T1 ATE212367T1 AT99902392T AT99902392T ATE212367T1 AT E212367 T1 ATE212367 T1 AT E212367T1 AT 99902392 T AT99902392 T AT 99902392T AT 99902392 T AT99902392 T AT 99902392T AT E212367 T1 ATE212367 T1 AT E212367T1
- Authority
- AT
- Austria
- Prior art keywords
- asymmetric cyanine
- cyanine dyes
- sub
- dye
- extinguishers
- Prior art date
Links
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 title abstract 4
- 239000000975 dye Substances 0.000 abstract 4
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 125000005647 linker group Chemical group 0.000 abstract 1
- 229910052760 oxygen Inorganic materials 0.000 abstract 1
- 229910052711 selenium Inorganic materials 0.000 abstract 1
- 229910052717 sulfur Inorganic materials 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Plural Heterocyclic Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Saccharide Compounds (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/012,525 US6080868A (en) | 1998-01-23 | 1998-01-23 | Nitro-substituted non-fluorescent asymmetric cyanine dye compounds |
PCT/US1999/001163 WO1999037717A1 (en) | 1998-01-23 | 1999-01-19 | Asymmetric cyanine dye quenchers |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE212367T1 true ATE212367T1 (de) | 2002-02-15 |
Family
ID=21755376
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT99902392T ATE212367T1 (de) | 1998-01-23 | 1999-01-19 | Asymmetrische cyaninfarbstoffe als fluoreszenzlöscher |
Country Status (8)
Country | Link |
---|---|
US (5) | US6080868A (de) |
EP (1) | EP1049744B1 (de) |
JP (2) | JP3946443B2 (de) |
AT (1) | ATE212367T1 (de) |
AU (1) | AU740989B2 (de) |
CA (1) | CA2318160C (de) |
DE (1) | DE69900813T2 (de) |
WO (1) | WO1999037717A1 (de) |
Families Citing this family (100)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5801155A (en) * | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
US5936087A (en) * | 1997-11-25 | 1999-08-10 | The Perkin-Elmer Corporation | Dibenzorhodamine dyes |
US6583168B1 (en) * | 1997-11-25 | 2003-06-24 | Applera Corporation | Sulfonated diarylrhodamine dyes |
US6080868A (en) | 1998-01-23 | 2000-06-27 | The Perkin-Elmer Corporation | Nitro-substituted non-fluorescent asymmetric cyanine dye compounds |
SE9902565D0 (sv) * | 1999-07-05 | 1999-07-05 | Jonas Karlsson | Probe for analysis of nucleic acids |
US7625705B2 (en) * | 1999-10-29 | 2009-12-01 | Hologic, Inc. | Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3′ flap |
US20070134686A1 (en) * | 1999-10-29 | 2007-06-14 | Stratagene California | Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3' flap |
JP2001204497A (ja) * | 2000-01-20 | 2001-07-31 | Japan Science & Technology Corp | 蛍光共鳴エネルギー移動を利用した、dnaハイブリダイゼーション法によるdna検出方法 |
TW572969B (en) * | 2000-02-10 | 2004-01-21 | Hayashibara Biochem Lab | Trimethine cyanine dye, light absorbent, light-resistant improver and optical recording medium containing same, and process for producing same |
US6323337B1 (en) | 2000-05-12 | 2001-11-27 | Molecular Probes, Inc. | Quenching oligonucleotides |
US6887664B2 (en) | 2000-06-06 | 2005-05-03 | Applera Corporation | Asynchronous primed PCR |
GB0024351D0 (en) * | 2000-10-04 | 2000-11-22 | Amersham Pharm Biotech Uk Ltd | Dye-labelled peptide and method |
AU2002213125B2 (en) * | 2000-10-11 | 2007-11-22 | Applied Biosystems, Llc. | Fluorescent nucleobase conjugates having anionic linkers |
DE60216468T2 (de) * | 2001-03-09 | 2007-09-27 | Boston Probes, Inc., Bedford | Kombinationsoligomere betreffende verfahren, kits und zusammensetzungen |
JP4676654B2 (ja) * | 2001-07-19 | 2011-04-27 | 富士フイルム株式会社 | 蛍光ヌクレオチド及びそれを用いた標識法 |
US20030082547A1 (en) | 2001-08-27 | 2003-05-01 | Ewing Gregory J. | Non-fluorescent quencher compounds and biomolecular assays |
US20050069962A1 (en) * | 2001-10-12 | 2005-03-31 | Archer Robert M | Antibody complexes and methods for immunolabeling |
US8323903B2 (en) * | 2001-10-12 | 2012-12-04 | Life Technologies Corporation | Antibody complexes and methods for immunolabeling |
US7166478B2 (en) * | 2002-03-12 | 2007-01-23 | Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. | Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses |
EP1543156A4 (de) * | 2002-07-18 | 2005-12-21 | Univ Leland Stanford Junior | Nachweis chemischer ligation unter verwendung von fluoreszenzquencher-abgangsgruppen |
JP4588451B2 (ja) * | 2002-09-08 | 2010-12-01 | アプライド バイオシステムズ, エルエルシー | Pnaダイマーおよびpnaオリゴマー合成に関する方法、組成物およびライブラリー |
US7803536B2 (en) | 2002-09-20 | 2010-09-28 | Integrated Dna Technologies, Inc. | Methods of detecting fluorescence with anthraquinone quencher dyes |
MXPA05010429A (es) | 2003-03-31 | 2005-11-04 | Hoffmann La Roche | Composiciones y metodos para detectar ciertos flavivirus que incluyen miembros del serogrupo del virus de la encefalitis japonesa. |
US7052412B2 (en) * | 2003-05-07 | 2006-05-30 | Bonneau Michael D | Load transfer arm for putter |
CA2445420A1 (en) * | 2003-07-29 | 2005-01-29 | Invitrogen Corporation | Kinase and phosphatase assays |
US7727752B2 (en) | 2003-07-29 | 2010-06-01 | Life Technologies Corporation | Kinase and phosphatase assays |
US7619059B2 (en) * | 2003-07-29 | 2009-11-17 | Life Technologies Corporation | Bimolecular optical probes |
US7271265B2 (en) | 2003-08-11 | 2007-09-18 | Invitrogen Corporation | Cyanine compounds and their application as quenching compounds |
EP2224243A1 (de) * | 2003-09-12 | 2010-09-01 | Life Technologies Corporation | Identifizierung von Modulatoren der enzymatischen Aktivität |
US7282339B2 (en) * | 2003-09-17 | 2007-10-16 | Invitrogen Corporation | Competitive immunoassay |
US20050118619A1 (en) * | 2003-09-22 | 2005-06-02 | Wensheng Xia | Dark quenchers for fluorescence resonance energy transfer (FRET) in bioassays |
WO2005049849A2 (en) | 2003-11-14 | 2005-06-02 | Integrated Dna Technologies, Inc. | Fluorescence quenching azo dyes, their methods of preparation and use |
US7776529B2 (en) | 2003-12-05 | 2010-08-17 | Life Technologies Corporation | Methine-substituted cyanine dye compounds |
WO2005056689A2 (en) | 2003-12-05 | 2005-06-23 | Molecular Probes, Inc. | Cyanine dye compounds |
WO2005077125A2 (en) * | 2004-02-11 | 2005-08-25 | Applera Corporation | Methods and compositions for detecting nucleic acids |
US8883486B2 (en) | 2004-05-04 | 2014-11-11 | Universite De Montreal | Arrestin biosensor |
US7939251B2 (en) | 2004-05-06 | 2011-05-10 | Roche Molecular Systems, Inc. | SENP1 as a marker for cancer |
US20060292649A1 (en) * | 2004-07-19 | 2006-12-28 | Cell Biosciences Inc. | Methods and apparatus for reference lab diagnostics |
US7935479B2 (en) * | 2004-07-19 | 2011-05-03 | Cell Biosciences, Inc. | Methods and devices for analyte detection |
US7846676B2 (en) * | 2004-07-19 | 2010-12-07 | Cell Biosciences, Inc. | Methods and devices for analyte detection |
US20060292558A1 (en) * | 2004-07-19 | 2006-12-28 | Cell Biosciences Inc. | Methods and apparatus for protein assay diagnostics |
EP2916128B1 (de) | 2004-07-19 | 2019-04-03 | ProteinSimple | Verfahren und vorrichtung zur analytdetektion |
JP5214967B2 (ja) * | 2004-08-13 | 2013-06-19 | エポック バイオサイエンシズ インコーポレーティッド | ホスホン酸蛍光色素および複合体 |
US7767834B2 (en) * | 2004-08-13 | 2010-08-03 | Elitech Holding B.V. | Phosphonylated fluorescent dyes and conjugates |
US7910753B2 (en) * | 2004-09-10 | 2011-03-22 | Anaspec Incorporated | Cyanine dyes and their applications as luminescence quenching compounds |
US8084260B2 (en) * | 2004-11-24 | 2011-12-27 | Applied Biosystems, Llc | Spectral calibration method and system for multiple instruments |
US20060140203A1 (en) * | 2004-12-28 | 2006-06-29 | Sanjeev Jain | System and method for packet queuing |
US20070015176A1 (en) * | 2005-02-18 | 2007-01-18 | Applera Corporation | Small nucleic acid detection probes and uses thereof |
WO2006094200A2 (en) * | 2005-03-03 | 2006-09-08 | The Trustees Of Boston College | Method of obtaining a desired localization for cellular imaging with the use of peptidoconjugates |
WO2006099537A2 (en) | 2005-03-15 | 2006-09-21 | Applera Corporation | The use of antibody-surrogate antigen systems for detection of analytes |
EP1872139A2 (de) * | 2005-04-09 | 2008-01-02 | Cell Biosciences, Inc. | Automatisches mikrovolumen-assaysystem |
CN101171343A (zh) | 2005-04-14 | 2008-04-30 | 阿普里拉股份有限公司 | 含有假异胞嘧啶核碱基衍生物的3’修饰寡核苷酸及其作为引物或探针的应用 |
EP1886145A2 (de) * | 2005-05-03 | 2008-02-13 | Applera Corporation | Fluoreszenznachweissystem und farbstoffsatz zur verwendung damit |
WO2006124816A1 (en) * | 2005-05-11 | 2006-11-23 | Molecular Probes, Inc. | Fluorescent chemical compounds having high selectivity for double stranded dna, and methods for their use |
US20060286378A1 (en) * | 2005-05-23 | 2006-12-21 | Shivkumar Chiruvolu | Nanostructured composite particles and corresponding processes |
US7569695B2 (en) * | 2005-05-24 | 2009-08-04 | Enzo Life Sciences, Inc. | Dyes for the detection or quantification of desirable target molecules |
US8357801B2 (en) | 2005-05-24 | 2013-01-22 | Enzo Life Sciences, Inc. | Labeling of target molecules, identification of organelles and other applications, novel compositions, methods and kits |
US7737281B2 (en) * | 2005-05-24 | 2010-06-15 | Enzo Life Sciences, Inc. C/O Enzo Biochem, Inc. | Purine based fluorescent dyes |
US20060292574A1 (en) * | 2005-05-26 | 2006-12-28 | Center for Disease Control, Department of Health, Taiwan, R.O.C. | Automated VNTR genotyping method |
US8227621B2 (en) | 2005-06-30 | 2012-07-24 | Li-Cor, Inc. | Cyanine dyes and methods of use |
US7630849B2 (en) * | 2005-09-01 | 2009-12-08 | Applied Biosystems, Llc | Method of automated calibration and diagnosis of laboratory instruments |
CN100361990C (zh) * | 2005-09-06 | 2008-01-16 | 武汉大学 | 含吲哚基团的二阶非线性光学发色团及其制法 |
US8945361B2 (en) * | 2005-09-20 | 2015-02-03 | ProteinSimple | Electrophoresis standards, methods and kits |
AU2005234696B2 (en) * | 2005-11-18 | 2012-02-16 | Phanos Technologies, Inc. | Fluorescent membrane intercalating probes and methods for their use |
US8092784B2 (en) * | 2005-11-30 | 2012-01-10 | Biotium, Inc. | Enzyme substrate comprising a functional dye and associated technology and methods |
WO2007106690A2 (en) | 2006-03-15 | 2007-09-20 | Siemens Healthcare Diagnostics Inc. | Degenerate nucleobase analogs |
JP2010500867A (ja) | 2006-06-30 | 2010-01-14 | ロゼッタ ジノミクス リミテッド | 核酸の検出方法 |
US8018593B2 (en) * | 2007-04-04 | 2011-09-13 | Netbio, Inc. | Integrated nucleic acid analysis |
CN101343420B (zh) * | 2007-07-12 | 2013-10-16 | 大连理工大学 | 一类不对称菁类荧光染料 |
US20090023156A1 (en) * | 2007-07-20 | 2009-01-22 | Voss Karl O | Methods and reagents for quantifying analytes |
WO2009067632A1 (en) * | 2007-11-20 | 2009-05-28 | Applied Biosystems Inc. | Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds |
WO2009067628A1 (en) | 2007-11-20 | 2009-05-28 | Applied Biosystems Inc. | Reversible di-nucleotide terminator sequencing |
US8501922B2 (en) * | 2008-02-07 | 2013-08-06 | Pacific Biosciences Of California, Inc. | CIS reactive oxygen quenchers integrated into linkers |
US7943320B2 (en) * | 2008-12-30 | 2011-05-17 | Canon U.S. Life Sciences, Inc. | Unsymmetrical cyanine dyes for high resolution nucleic acid melting analysis |
US8332007B2 (en) * | 2009-01-12 | 2012-12-11 | The Board Of Trustees Of The University Of Illinois | Quantitative three-dimensional mapping of oxygen tension |
WO2010117420A2 (en) | 2009-03-30 | 2010-10-14 | Pacific Biosciences Of California, Inc. | Fret-labeled compounds and uses therefor |
GB0915664D0 (en) | 2009-09-08 | 2009-10-07 | Enigma Diagnostics Ltd | Reaction method |
US9506057B2 (en) | 2010-03-26 | 2016-11-29 | Integrated Dna Technologies, Inc. | Modifications for antisense compounds |
AU2011230496B2 (en) | 2010-03-26 | 2015-09-17 | Integrated Dna Technologies, Inc. | Methods for enhancing nucleic acid hybridization |
WO2012033848A1 (en) | 2010-09-07 | 2012-03-15 | Integrated Dna Technologies, Inc. | Modifications for antisense compounds |
US8968875B2 (en) | 2012-03-21 | 2015-03-03 | Larry Takiff | Eyewear including nitrophenyl functionalized boron pyrromethene dye for neutralizing laser threat |
US8486311B1 (en) | 2012-03-21 | 2013-07-16 | Revision Military, Ltd. | Eyewear including catechol functionalized boron pyrromethene dye for neutralizing laser threat |
US9709500B2 (en) | 2012-05-02 | 2017-07-18 | Charles River Laboratories, Inc. | Optical method for detecting viable microorganisms in a cell sample |
EP2769218B1 (de) | 2012-05-02 | 2016-08-10 | Charles River Laboratories, Inc. | Lebensfähigkeitsfärbungsverfahren |
US10324036B2 (en) | 2012-05-02 | 2019-06-18 | Charles River Laboratories, Inc. | Porous planar cell capture system |
WO2016069922A1 (en) | 2014-10-29 | 2016-05-06 | Biotium, Inc. | Nucleic acid modifying agents and uses thereof |
NL2013786B1 (en) * | 2014-11-13 | 2016-10-07 | Original G B V | Quenched coating. |
US9957393B2 (en) | 2015-03-30 | 2018-05-01 | Enzo Biochem, Inc. | Monoazo dyes with cyclic amine as fluorescence quenchers |
CN108463560B (zh) | 2016-01-15 | 2022-09-20 | 昆塔波尔公司 | 具有降低的背景的基于光学的纳米孔分析 |
BR112018073378A2 (pt) | 2016-05-20 | 2019-03-06 | Quantum-Si Incorporated | composições de nucleotídeos marcados e métodos para sequenciamento de ácidos nucleicos |
CA3031812A1 (en) | 2016-08-19 | 2018-02-22 | Quantapore, Inc. | Optically-based nanopore sequencing using quenching agents |
WO2018162986A2 (en) | 2017-03-09 | 2018-09-13 | Elitechgroup B.V. | Nitrodiarylethenes as fluorescence quenchers for nucleic acid probes |
ES2909351T3 (es) | 2017-08-17 | 2022-05-06 | Elitechgroup Inc | Quencheres estabilizadores de fluorescencia dúplex para sondas de ácidos nucleicos |
CN109053778A (zh) * | 2018-06-14 | 2018-12-21 | 忻州师范学院 | 特异性检测氰根的试剂及其合成方法和应用 |
US20210310002A1 (en) * | 2018-08-31 | 2021-10-07 | City Of Hope | Cationic compounds for delivery of nucleic acids |
EP4047057A4 (de) * | 2019-11-19 | 2024-01-17 | SFC Co., Ltd. | Löscher und seine verwendungen |
US20230075533A1 (en) | 2021-09-03 | 2023-03-09 | ProteinSimple | Methods and systems for analysis of samples containing particles used for gene delivery |
AU2022379602B2 (en) | 2021-10-26 | 2024-09-12 | Caribou Biosciences, Inc. | Exonuclease-coupled real-time endonuclease activity assay |
CN116003448B (zh) * | 2022-11-07 | 2024-06-11 | 淮阴工学院 | 一种含吲哚啉多烯键识别Aβ纤维的BODIPY近红外荧光探针及其制备方法 |
WO2024170742A1 (en) | 2023-02-17 | 2024-08-22 | Danmarks Tekniske Universitet | Copolymer-drug conjugate for treatment of tumours |
Family Cites Families (44)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2078233A (en) * | 1934-09-14 | 1937-04-27 | Eastman Kodak Co | Photographic emulsion |
GB455710A (en) * | 1935-03-21 | 1936-10-21 | Kodak Ltd | Improvements in the manufacture of cyanine and related dyes |
US2152615A (en) * | 1936-08-29 | 1939-03-28 | Agfa Ansco Corp | Pentamethine-omega-aldehydes of heterocyclic bases |
US2269234A (en) | 1940-10-18 | 1942-01-06 | Eastman Kodak Co | Polymethine dye intermediates |
US4230558A (en) | 1978-10-02 | 1980-10-28 | Coulter Electronics, Inc. | Single drop separator |
US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
US4439356A (en) | 1981-03-03 | 1984-03-27 | Syva Company | Unsymmetrical fluorescein derivatives |
US4415732A (en) | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
US4711955A (en) | 1981-04-17 | 1987-12-08 | Yale University | Modified nucleotides and methods of preparing and using same |
CA1190838A (en) | 1981-07-17 | 1985-07-23 | Cavit Akin | Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer |
US4757141A (en) | 1985-08-26 | 1988-07-12 | Applied Biosystems, Incorporated | Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof |
US5258538A (en) | 1985-08-26 | 1993-11-02 | Applied Biosystems, Inc. | 2,3-disubstituted-1,3,2-oxazaphosphacycloalkanes as nucleic acid linking agents |
US4883867A (en) * | 1985-11-01 | 1989-11-28 | Becton, Dickinson And Company | Detection of reticulocytes, RNA or DNA |
ATE88761T1 (de) | 1986-01-10 | 1993-05-15 | Amoco Corp | Kompetitiver homogener test. |
US4855225A (en) | 1986-02-07 | 1989-08-08 | Applied Biosystems, Inc. | Method of detecting electrophoretically separated oligonucleotides |
US4811218A (en) | 1986-06-02 | 1989-03-07 | Applied Biosystems, Inc. | Real time scanning electrophoresis apparatus for DNA sequencing |
JP2527340B2 (ja) | 1986-12-15 | 1996-08-21 | アプライド バイオシステムズ インコーポレイテッド | ロ―ダミン染料の5−及び6−スクシニミジルカルボキシレ―ト異性体 |
US5231191A (en) | 1987-12-24 | 1993-07-27 | Applied Biosystems, Inc. | Rhodamine phosphoramidite compounds |
US5188934A (en) | 1989-11-14 | 1993-02-23 | Applied Biosystems, Inc. | 4,7-dichlorofluorescein dyes as molecular probes |
US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
EP0605655B1 (de) * | 1991-09-16 | 1997-05-07 | Molecular Probes, Inc. | Dimere unsymmetrische Cyaninfarbstoffe |
US5321130A (en) * | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
EP0601889A2 (de) | 1992-12-10 | 1994-06-15 | Maine Medical Center Research Institute | Nukleinsäure-Sonden |
US5410030A (en) * | 1993-04-05 | 1995-04-25 | Molecular Probes, Inc. | Dimers of unsymmetrical cyanine dyes containing pyridinium moieties |
US5436134A (en) * | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
US5658751A (en) * | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
US5534416A (en) * | 1993-04-13 | 1996-07-09 | Molecular Probes, Inc. | Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
US5654419A (en) | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
US5597696A (en) * | 1994-07-18 | 1997-01-28 | Becton Dickinson And Company | Covalent cyanine dye oligonucleotide conjugates |
US5565554A (en) * | 1994-07-29 | 1996-10-15 | The Regents Of The University Of California | Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores |
JP3189000B2 (ja) * | 1994-12-01 | 2001-07-16 | 東ソー株式会社 | 特定核酸配列の検出方法 |
US5656449A (en) * | 1995-03-06 | 1997-08-12 | Molecular Probes, Inc. | Neutral unsymmetrical cyanine dyes |
EP0826066B1 (de) | 1995-05-05 | 2000-09-13 | The Perkin-Elmer Corporation | Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay |
US5658735A (en) * | 1995-11-09 | 1997-08-19 | Biometric Imaging, Inc. | Cyclized fluorescent nucleic acid intercalating cyanine dyes and nucleic acid detection methods |
JPH09288326A (ja) * | 1996-04-23 | 1997-11-04 | Fuji Photo Film Co Ltd | 熱現像感光材料 |
US5863727A (en) | 1996-05-03 | 1999-01-26 | The Perkin-Elmer Corporation | Energy transfer dyes with enhanced fluorescence |
US5847162A (en) | 1996-06-27 | 1998-12-08 | The Perkin Elmer Corporation | 4, 7-Dichlororhodamine dyes |
SE506700C2 (sv) * | 1996-05-31 | 1998-02-02 | Mikael Kubista | Sond och förfaranden för analys av nukleinsyra |
US5736333A (en) | 1996-06-04 | 1998-04-07 | The Perkin-Elmer Corporation | Passive internal references for the detection of nucleic acid amplification products |
US5821356A (en) | 1996-08-12 | 1998-10-13 | The Perkin Elmer Corporation | Propargylethoxyamino nucleotides |
US5770716A (en) | 1997-04-10 | 1998-06-23 | The Perkin-Elmer Corporation | Substituted propargylethoxyamido nucleosides, oligonucleotides and methods for using same |
US6080868A (en) | 1998-01-23 | 2000-06-27 | The Perkin-Elmer Corporation | Nitro-substituted non-fluorescent asymmetric cyanine dye compounds |
AU5728400A (en) | 1999-06-09 | 2000-12-28 | Amersham Pharmacia Biotech Uk Limited | Ph sensitive cyanine dyes as reactive fluorescent reagents |
-
1998
- 1998-01-23 US US09/012,525 patent/US6080868A/en not_active Expired - Lifetime
-
1999
- 1999-01-19 CA CA002318160A patent/CA2318160C/en not_active Expired - Fee Related
- 1999-01-19 AU AU22383/99A patent/AU740989B2/en not_active Ceased
- 1999-01-19 WO PCT/US1999/001163 patent/WO1999037717A1/en active IP Right Grant
- 1999-01-19 DE DE69900813T patent/DE69900813T2/de not_active Expired - Lifetime
- 1999-01-19 AT AT99902392T patent/ATE212367T1/de not_active IP Right Cessation
- 1999-01-19 JP JP2000528627A patent/JP3946443B2/ja not_active Expired - Lifetime
- 1999-01-19 EP EP99902392A patent/EP1049744B1/de not_active Expired - Lifetime
- 1999-07-20 US US09/357,740 patent/US6348596B1/en not_active Expired - Lifetime
-
2000
- 2000-06-21 US US09/602,544 patent/US6541618B1/en not_active Expired - Lifetime
-
2003
- 2003-03-31 US US10/404,404 patent/US6750024B2/en not_active Expired - Lifetime
-
2004
- 2004-04-08 JP JP2004114829A patent/JP4471711B2/ja not_active Expired - Lifetime
- 2004-05-05 US US10/840,189 patent/US7166715B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US6080868A (en) | 2000-06-27 |
JP2002501102A (ja) | 2002-01-15 |
JP2004323839A (ja) | 2004-11-18 |
DE69900813T2 (de) | 2002-10-02 |
US6348596B1 (en) | 2002-02-19 |
CA2318160C (en) | 2005-11-29 |
US20030207305A1 (en) | 2003-11-06 |
AU2238399A (en) | 1999-08-09 |
CA2318160A1 (en) | 1999-07-29 |
US7166715B2 (en) | 2007-01-23 |
EP1049744A1 (de) | 2000-11-08 |
AU740989B2 (en) | 2001-11-22 |
US6541618B1 (en) | 2003-04-01 |
WO1999037717A1 (en) | 1999-07-29 |
US6750024B2 (en) | 2004-06-15 |
JP3946443B2 (ja) | 2007-07-18 |
EP1049744B1 (de) | 2002-01-23 |
DE69900813D1 (de) | 2002-03-14 |
JP4471711B2 (ja) | 2010-06-02 |
US20050009058A1 (en) | 2005-01-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE212367T1 (de) | Asymmetrische cyaninfarbstoffe als fluoreszenzlöscher | |
ATE370935T1 (de) | Rylenderivate und deren verwendung als farbstoffe | |
ATE315066T1 (de) | Cyaninfarbstoffe | |
WO2005056689A3 (en) | Cyanine dye compounds | |
ATE216718T1 (de) | Fluoreszente zusammensetzungen und ihre anwendung | |
DE60131276D1 (de) | Kryptate der seltenen erden mit verringerter fluoreszenz-löschung | |
KR950032501A (ko) | 분산 염료 조성물 및 이를 사용한 소수성 섬유물질의 염색방법. | |
TR199902897T2 (xx) | S�v� benz-izo-kuinolin t�revleri. | |
HUP9800008A2 (hu) | Színezőreagens vérsejtek meghatározására | |
EA199800683A1 (ru) | Стабильные гербицидные композиции, содержащие хелаты гербицидных дионовых соединений с металлами | |
FI853586A0 (fi) | Nya arylaettiksyraderivat. | |
ATE223461T1 (de) | Helium-neon anregbare retikulozitfarbstoffe erhältlich aus halolepidinen | |
EA200000092A1 (ru) | Противоаллергические средства | |
KR970065651A (ko) | 섬유-반응성 디스아조 염료로 이루어진 염료 혼합물 | |
TR200001027T2 (tr) | Yüksek molaküler ağırlıklı ester yağı içeren saç şekillendiricisi karışımları. | |
ID24362A (id) | Senyawa monoazo berpenghubung | |
TR199700481A2 (xx) | Sefem ve izooksafem t�revlerinin haz�rlanmas�. | |
KR960704069A (ko) | 사람 시토크롬 p4501a2 유전자 다형의 검출방법 | |
KR890004994A (ko) | 신규 백금 착화합물 | |
KR950003354A (ko) | 디케토피롤로피롤 안료로 대량 착색된 폴리아미드 | |
KR940014682A (ko) | 벤조디푸라논 화합물을 함유한 조성물 및 이를 사용하는 소수성 재료의 염색방법 | |
KR930000616A (ko) | 모노아조염료 | |
JP2002294094A5 (de) | ||
KR960014360A (ko) | 동물유전자를 이용한 형질전환된 식물세포 선별방법 | |
KR970015680A (ko) | 흑색 분산 염료 조성물 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |