AR096027A2 - Proteínas insecticidas quiméricas de bacillus thuringiensis - Google Patents
Proteínas insecticidas quiméricas de bacillus thuringiensisInfo
- Publication number
- AR096027A2 AR096027A2 ARP140101586A ARP140101586A AR096027A2 AR 096027 A2 AR096027 A2 AR 096027A2 AR P140101586 A ARP140101586 A AR P140101586A AR P140101586 A ARP140101586 A AR P140101586A AR 096027 A2 AR096027 A2 AR 096027A2
- Authority
- AR
- Argentina
- Prior art keywords
- amino acid
- acid sequence
- proteins
- lmbp
- cry2ae
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 11
- 102000004169 proteins and genes Human genes 0.000 title abstract 9
- 241000193388 Bacillus thuringiensis Species 0.000 title abstract 2
- 229940097012 bacillus thuringiensis Drugs 0.000 title abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 7
- 230000000749 insecticidal effect Effects 0.000 abstract 4
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 3
- 108091026890 Coding region Proteins 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 2
- 150000007523 nucleic acids Chemical group 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000002917 insecticide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Compuestos insecticidas derivados de cepas de Bacillus thuringiensis. Se proveen proteínas denominadas Cry2Ae, Cry2Af y Cry2Ag, y variantes de las mismas, así como secuencias de ADN codificadores de estas proteínas o de las variantes de las mismas. Además se proveen huéspedes recombinantes que expresan dichas proteínas, en particular células vegetales y plantas. Reivindicación 1: Un gen quimérico que comprende una secuencia de ácido nucleico bajo el control de un promotor de expresión en plantas, caracterizada porque dicha secuencia de ácido nucleico codifica una proteína insecticida Cry2Ae que comprende una secuencia de aminoácidos seleccionada del grupo formado por : a) la secuencia de aminoácidos de la proteína codificada por el gen cry2Ae depositado en BCCM-LMBP con el Nº Acceso LMBP 4248 o una porción insecticida de la misma, b) la secuencia de aminoácidos de la proteína de la SEQ ID Nº 2, o una porción insecticida de la misma, c) la secuencia de aminoácidos de la proteína de la SEQ ID Nº 2 entre la posición de aminoácido 1 y la posición de aminoácido 625 y d) la secuencia de aminoácidos de una proteína insecticida codificada por un ADN que se hibridiza con la secuencia de codificación cry2Ae depositada en BCCM-LMBP con el Nº Acceso LMBP 4248 bajo condiciones de hibridación severas, donde dicho ADN presenta una identidad de secuencia de por lo menos 93%, en particular por lo menos 98%, preferentemente por lo menos 99%, con la secuencia de codificación de la SEQ ID Nº 1.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US75629601A | 2001-01-09 | 2001-01-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR096027A2 true AR096027A2 (es) | 2015-12-02 |
Family
ID=25042854
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP020100051A AR032231A1 (es) | 2001-01-09 | 2002-01-09 | Nuevas proteinas insecticidas de bacillus thuringiensis |
| ARP140101586A AR096027A2 (es) | 2001-01-09 | 2014-04-15 | Proteínas insecticidas quiméricas de bacillus thuringiensis |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP020100051A AR032231A1 (es) | 2001-01-09 | 2002-01-09 | Nuevas proteinas insecticidas de bacillus thuringiensis |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP1352068B1 (es) |
| JP (1) | JP4404549B2 (es) |
| CN (1) | CN1234867C (es) |
| AR (2) | AR032231A1 (es) |
| AT (1) | ATE409231T1 (es) |
| AU (1) | AU2002252974B2 (es) |
| BR (3) | BR122015003517B1 (es) |
| CA (2) | CA2771117C (es) |
| DE (1) | DE60229037D1 (es) |
| ES (1) | ES2315360T3 (es) |
| MX (1) | MXPA03006130A (es) |
| PT (1) | PT1352068E (es) |
| SI (1) | SI1352068T1 (es) |
| WO (1) | WO2002057664A2 (es) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR0014516A (pt) | 1999-09-15 | 2002-07-02 | Monsanto Technology Llc | Composições e métodos de uso de delta-endotoxina do bacilo thuringiensis ativo de lepidopteran |
| CN100497378C (zh) | 2002-03-22 | 2009-06-10 | 拜尔生物科学公司 | 新的苏云金芽孢杆菌杀昆虫蛋白质 |
| WO2005082077A2 (en) | 2004-02-25 | 2005-09-09 | Pioneer Hi-Bred International, Inc. | Novel bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof |
| AU2006231503A1 (en) * | 2005-04-05 | 2006-10-12 | E. I. Du Pont De Nemours And Company | Methods and compositions for designing nucleic acid molecules for polypeptide expression in plants using plant virus codon-bias |
| EP1904521B1 (en) | 2005-07-08 | 2013-08-21 | Universidad Nacional Autonoma De Mexico Instituto | Novel bacterial proteins with pesticidal activity |
| CN101405296B (zh) * | 2006-03-21 | 2014-04-30 | 拜尔作物科学公司 | 编码杀虫蛋白的新基因 |
| CN101688216B (zh) * | 2007-06-01 | 2014-03-26 | 拜尔作物科学公司 | 编码杀虫蛋白质的新基因 |
| EA018012B1 (ru) * | 2007-06-11 | 2013-04-30 | Байер Кропсайенс Н.В. | Устойчивые к насекомым растения хлопчатника и методы их идентификации |
| US7772465B2 (en) | 2007-06-26 | 2010-08-10 | Pioneer Hi-Bred International, Inc. | Bacillus thuringiensis gene with lepidopteran activity |
| CA2956841A1 (en) * | 2007-10-16 | 2009-04-23 | Athenix Corporation | Axmi-066 and axmi-076: delta-endotoxin proteins and methods for their use |
| WO2009149787A1 (en) * | 2008-06-13 | 2009-12-17 | Bayer Bioscience N.V. | Bollworm insect resistance management in transgenic plants |
| MX2011009496A (es) * | 2009-03-11 | 2011-10-14 | Athenix Corp | Axmi-001, axmi-002, axmi-030, axmi-035 y axmi-045: genes de toxina y metodos para su uso. |
| EP2512223B1 (en) * | 2009-12-16 | 2018-01-24 | Dow AgroSciences LLC | Insecticidal protein combination comprising cry1ab and cry2aa for controlling european corn borer, and methods for insect resistance management |
| MX2012007360A (es) | 2009-12-23 | 2012-11-06 | Bayer Ip Gmbh | Plantas tolerantes a herbicidas inhibidores de las hppd. |
| UY33140A (es) | 2009-12-23 | 2011-07-29 | Bayer Cropscience Ag | Plantas tolerantes a herbicidas inhibidores de las hppd |
| WO2011076889A1 (en) | 2009-12-23 | 2011-06-30 | Bayer Cropscience Ag | Plants tolerant to hppd inhibitor herbicides |
| WO2011076885A1 (en) | 2009-12-23 | 2011-06-30 | Bayer Cropscience Ag | Plants tolerant to hppd inhibitor herbicides |
| ES2658990T3 (es) | 2009-12-23 | 2018-03-13 | Bayer Intellectual Property Gmbh | Plantas tolerantes a herbicidas inhibidores de HPPD |
| EP2453012B1 (en) | 2010-11-10 | 2016-06-01 | Bayer CropScience AG | HPPD variants and methods of use |
| US8648230B2 (en) | 2011-03-18 | 2014-02-11 | Ms Technologies, Llc | Regulatory regions preferentially expressing in non-pollen plant tissue |
| EP2688407B1 (en) | 2011-03-25 | 2015-04-22 | Bayer Intellectual Property GmbH | Use of n-(1,2,5-oxadiazol-3-yl)benzamides for controlling unwanted plants in areas of transgenic crop plants being tolerant to hppd inhibitor herbicides |
| JP5847921B2 (ja) | 2011-03-25 | 2016-01-27 | バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングＢａｙｅｒ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ ＧｍｂＨ | Ｈｐｐｄ阻害薬型除草剤に対して耐性であるトランスジェニック作物の区域で望ましくない植物を防除するためのｎ−（テトラゾール−４−イル）−もしくはｎ−（トリアゾール−３−イル）アリールカルボキサミド類またはそれらの塩の使用 |
| CN112400900A (zh) | 2012-03-09 | 2021-02-26 | 韦斯塔隆公司 | 毒肽产生、植物中的肽表达和富含半胱氨酸的肽的组合 |
| US11692016B2 (en) | 2012-03-09 | 2023-07-04 | Vestaron Corporation | High gene expression yeast strain |
| CN103571853B (zh) * | 2012-08-08 | 2016-01-20 | 中国科学院亚热带农业生态研究所 | 密码子优化的Cry2Aa基因与重组载体以及改变作物抗性的方法 |
| AR092564A1 (es) | 2012-09-14 | 2015-04-22 | Bayer Cropscience Lp | Variantes de la enzima 4-hidroxifenil piruvato deoxigenasa (hppd) y metodos de uso para conferir tolerancia a herbicidas en plantas |
| BR112015021651B1 (pt) | 2013-03-07 | 2021-12-28 | Bayer Cropscience Lp | Molécula de ácido nucleico recombinante, cassete de expressão, célula hospedeira de bactéria, polipeptídeo recombinante, composição e métodos para aplicação dos mesmos |
| CN103290130B (zh) * | 2013-06-13 | 2015-02-04 | 中华人民共和国上海出入境检验检疫局 | 转基因产品中Cry2Ae基因的检测方法及其试剂盒 |
| BR112016020889B1 (pt) | 2014-03-11 | 2022-10-04 | BASF Agricultural Solutions Seed US LLC | Molécula de ácido nucleico recombinante, célula hospedeira bacteriana, proteína hppd recombinante, uso do ácido nucleico recombinante e produto de base |
| CN104286014B (zh) * | 2014-08-27 | 2016-03-23 | 北京大北农科技集团股份有限公司 | 杀虫蛋白的用途 |
| CN105367633B (zh) * | 2014-08-28 | 2018-11-30 | 四川农业大学 | 一种BT蛋白CRY2Ab32、其编码基因及应用 |
| AR105155A1 (es) * | 2015-07-07 | 2017-09-13 | Syngenta Participations Ag | Composiciones y métodos para controlar plagas de plantas |
| ES2933673T3 (es) | 2015-09-11 | 2023-02-13 | BASF Agricultural Solutions Seed US LLC | Variantes de HPPD y métodos de uso |
| US11535653B2 (en) | 2016-10-21 | 2022-12-27 | Vestaron Corporation | Cleavable peptides and insecticidal and nematicidal proteins comprising same |
| CN110267975B (zh) | 2016-11-23 | 2024-04-19 | 巴斯夫农业种子解决方案美国有限责任公司 | Axmi669和axmi991毒素基因及其使用方法 |
| WO2018119336A1 (en) | 2016-12-22 | 2018-06-28 | Athenix Corp. | Use of cry14 for the control of nematode pests |
| UY37571A (es) | 2017-01-18 | 2018-08-31 | Bayer Cropscience Lp | Gen de toxina bp005 y procedimientos para su uso |
| AR110756A1 (es) | 2017-01-18 | 2019-05-02 | Bayer Cropscience Lp | Uso de bp005 para el control de patógenos de planta |
| WO2018165091A1 (en) | 2017-03-07 | 2018-09-13 | Bayer Cropscience Lp | Hppd variants and methods of use |
| BR112020008096A2 (pt) | 2017-10-24 | 2020-11-03 | Basf Se | método para conferir tolerância a um herbicida e planta de soja transgênica |
| WO2019083810A1 (en) | 2017-10-24 | 2019-05-02 | Basf Se | IMPROVING HERBICIDE TOLERANCE FOR 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE (HPPD) INHIBITORS BY NEGATIVE REGULATION OF HPPD EXPRESSION IN SOYBEANS |
| PH12021552729A1 (en) * | 2019-04-24 | 2022-07-11 | Dcm Shriram Ltd | Codon optimized synthetic nucleotide sequences encoding cry2ai protein and uses thereof |
| US20220275031A1 (en) * | 2019-07-30 | 2022-09-01 | Dcm Shriram Limited | Synthetic nucleotide sequences encoding insecticidal crystal protein and uses thereof |
| CA3157234A1 (en) | 2019-10-14 | 2021-04-22 | BASF Agricultural Solutions Seed US LLC | Novel insect resistant genes and methods of use |
| BR112022007119A2 (pt) | 2019-10-14 | 2022-07-05 | BASF Agricultural Solutions Seed US LLC | Molécula de ácido nucleico, ácido nucleico, polipeptídeos, vetor, célula hospedeira, planta transgênica, semente transgênica, composição, métodos para controlar uma população de pragas, para matar uma praga, para produzir um polipeptídeo, para proteger uma planta e para aumentar o rendimento em uma planta, planta, uso do ácido nucleico e produto básico |
| CN111334586A (zh) * | 2020-04-02 | 2020-06-26 | 南京农业大学 | 基于as-pcr技术快速检测二化螟鱼尼丁受体基因突变的方法 |
| UY39585A (es) * | 2020-12-23 | 2022-07-29 | Monsanto Technology Llc | Proteínas que exhiben actividad inhibidora de insectos frente a plagas con importancia agrícola de plantas de cultivo y semillas |
| CN114717256A (zh) * | 2022-02-19 | 2022-07-08 | 四川农业大学 | 在水稻中高效表达Bt蛋自Cry2Ag1抗草地贪夜蛾的方法 |
| WO2024137446A1 (en) | 2022-12-19 | 2024-06-27 | BASF Agricultural Solutions Seed US LLC | Methods of identifying and evaluating genes for insect control |
| WO2024137438A2 (en) | 2022-12-19 | 2024-06-27 | BASF Agricultural Solutions Seed US LLC | Insect toxin genes and methods for their use |
| WO2024137445A1 (en) | 2022-12-20 | 2024-06-27 | BASF Agricultural Solutions Seed US LLC | Methods of identifying and evaluating genes for insect control |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL177516C (nl) | 1978-09-12 | 1985-10-01 | Pidou Bv | Afdichtmanchet. |
| NL8700204A (nl) | 1987-01-28 | 1988-08-16 | Pidou Bv | Afdichtinrichting. |
| WO1998040490A1 (en) * | 1997-03-13 | 1998-09-17 | Mycogen Corporation | Bacillus thuringiensis toxins |
| US6489542B1 (en) * | 1998-11-04 | 2002-12-03 | Monsanto Technology Llc | Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids |
| BR0014516A (pt) * | 1999-09-15 | 2002-07-02 | Monsanto Technology Llc | Composições e métodos de uso de delta-endotoxina do bacilo thuringiensis ativo de lepidopteran |
-
2002
- 2002-01-08 PT PT02722023T patent/PT1352068E/pt unknown
- 2002-01-08 JP JP2002557703A patent/JP4404549B2/ja not_active Expired - Lifetime
- 2002-01-08 MX MXPA03006130A patent/MXPA03006130A/es active IP Right Grant
- 2002-01-08 BR BR122015003517-1A patent/BR122015003517B1/pt active IP Right Grant
- 2002-01-08 BR BRPI0206346-8A patent/BRPI0206346B1/pt unknown
- 2002-01-08 CA CA2771117A patent/CA2771117C/en not_active Expired - Lifetime
- 2002-01-08 CN CNB028035674A patent/CN1234867C/zh not_active Expired - Lifetime
- 2002-01-08 CA CA2433817A patent/CA2433817C/en not_active Expired - Lifetime
- 2002-01-08 SI SI200230777T patent/SI1352068T1/sl unknown
- 2002-01-08 ES ES02722023T patent/ES2315360T3/es not_active Expired - Lifetime
- 2002-01-08 AT AT02722023T patent/ATE409231T1/de active
- 2002-01-08 DE DE60229037T patent/DE60229037D1/de not_active Expired - Lifetime
- 2002-01-08 BR BR0206346-8A patent/BR0206346A/pt active IP Right Grant
- 2002-01-08 EP EP02722023A patent/EP1352068B1/en not_active Expired - Lifetime
- 2002-01-08 AU AU2002252974A patent/AU2002252974B2/en not_active Expired
- 2002-01-08 WO PCT/EP2002/000298 patent/WO2002057664A2/en active IP Right Grant
- 2002-01-09 AR ARP020100051A patent/AR032231A1/es active IP Right Grant
-
2014
- 2014-04-15 AR ARP140101586A patent/AR096027A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BR122015003517B1 (pt) | 2021-02-02 |
| CN1484702A (zh) | 2004-03-24 |
| DE60229037D1 (de) | 2008-11-06 |
| AR032231A1 (es) | 2003-10-29 |
| CA2433817C (en) | 2012-05-22 |
| CA2771117C (en) | 2015-03-17 |
| WO2002057664A2 (en) | 2002-07-25 |
| BR0206346A (pt) | 2003-10-28 |
| AU2002252974B2 (en) | 2006-12-14 |
| PT1352068E (pt) | 2009-01-06 |
| WO2002057664A3 (en) | 2003-04-17 |
| EP1352068B1 (en) | 2008-09-24 |
| CA2771117A1 (en) | 2002-07-25 |
| EP1352068A2 (en) | 2003-10-15 |
| JP4404549B2 (ja) | 2010-01-27 |
| CN1234867C (zh) | 2006-01-04 |
| JP2004522437A (ja) | 2004-07-29 |
| MXPA03006130A (es) | 2005-02-14 |
| BRPI0206346B1 (pt) | 2018-04-03 |
| ATE409231T1 (de) | 2008-10-15 |
| CA2433817A1 (en) | 2002-07-25 |
| ES2315360T3 (es) | 2009-04-01 |
| SI1352068T1 (sl) | 2009-02-28 |
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| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |