AR046944A1 - Composiciones de genes y proteinas insecticidas secretadas de bacillus thuringiensis y usos de las mismas - Google Patents
Composiciones de genes y proteinas insecticidas secretadas de bacillus thuringiensis y usos de las mismasInfo
- Publication number
- AR046944A1 AR046944A1 ARP040104700A ARP040104700A AR046944A1 AR 046944 A1 AR046944 A1 AR 046944A1 AR P040104700 A ARP040104700 A AR P040104700A AR P040104700 A ARP040104700 A AR P040104700A AR 046944 A1 AR046944 A1 AR 046944A1
- Authority
- AR
- Argentina
- Prior art keywords
- seq
- insecticidal
- bacillus thuringiensis
- proteins
- nucleotide sequences
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 241000193388 Bacillus thuringiensis Species 0.000 title abstract 4
- 229940097012 bacillus thuringiensis Drugs 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 239000000203 mixture Substances 0.000 title 1
- 230000000749 insecticidal effect Effects 0.000 abstract 7
- 241000255777 Lepidoptera Species 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 3
- 108700012359 toxins Proteins 0.000 abstract 3
- 241001147398 Ostrinia nubilalis Species 0.000 abstract 2
- 241000500437 Plutella xylostella Species 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 241000196324 Embryophyta Species 0.000 abstract 1
- 241000985245 Spodoptera litura Species 0.000 abstract 1
- 241000607479 Yersinia pestis Species 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 238000012512 characterization method Methods 0.000 abstract 1
- 239000012228 culture supernatant Substances 0.000 abstract 1
- 210000001723 extracellular space Anatomy 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 231100000765 toxin Toxicity 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Insects & Arthropods (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Aislamiento y caracterizacion de secuencias de nucleotidos que codifican proteínas insecticidas secretadas hacia el espacio extracelular de Bacillus thuringiensis y cepas relacionadas. Las proteínas se aíslan de sobrenadantes de cultivo de Bacillus thuringiensis y cepas relacionadas y presentan actividad insecticida contra insectos lepidopteros incluyendo el barrenador del maíz europeo (ECB), la oruga capullera de tabaco (TBW) y la pollilla de dorso de diamante (DBM). Se describen proteínas insecticidas codificadas por las secuencias de nucleotidos que se hibridizan bajo condiciones severas con las secuencias de nucleotidos aisladas y caracterizadas. Se describen métodos para elaborar y usar células y plantas transgénicas que comprenden dicha secuencia de nucleotidos. Reivindicacion 1: Un polinucleotido aislado, caracterizado porque codifica una proteína toxina insecticida de Bacillus thuringiensis o un fragmento insecticida de la misma, activa contra una plaga de insectos lepidopteros, donde dicha proteína toxina insecticida comprende una secuencia de polipéptidos que presente al menos un 80% aproximadamente de identidad de secuencia con una secuencia de polipéptidos seleccionada del grupo formado por las SEQ ID Ns 4 (TIC900), SEQ ID Ns 6 (TIC402), SEQ ID Ns 8 (TIC403), SEQ ID Ns 10(TIC404), SEQ ID Ns 30 (TIC434), SEQ ID Ns 12 (TIC961), SEQ ID Ns 14 (TIC962), SEQ ID Ns 16(TIC963), SEQ ID Ns 18 (TIC965) y SEQ ID Ns 20(TIC966), o una variante de toxina insecticida activa contra lepidopteros o una porcion de la misma.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US52991703P | 2003-12-16 | 2003-12-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR046944A1 true AR046944A1 (es) | 2006-01-04 |
Family
ID=35320635
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP040104700A AR046944A1 (es) | 2003-12-16 | 2004-12-16 | Composiciones de genes y proteinas insecticidas secretadas de bacillus thuringiensis y usos de las mismas |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US20080040827A1 (es) |
| EP (1) | EP1708561B1 (es) |
| CN (1) | CN101133079A (es) |
| AR (1) | AR046944A1 (es) |
| AT (1) | ATE487372T1 (es) |
| BR (1) | BRPI0417735A (es) |
| CA (1) | CA2547933C (es) |
| DE (1) | DE602004030044D1 (es) |
| ES (1) | ES2353603T3 (es) |
| MX (1) | MXPA06007005A (es) |
| WO (1) | WO2005107383A2 (es) |
| ZA (1) | ZA200604678B (es) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7355099B2 (en) | 2003-02-20 | 2008-04-08 | Athenix Corporation | AXMI-004, a delta-endotoxin gene and methods for its use |
| US20080040827A1 (en) | 2003-12-16 | 2008-02-14 | Judith Donovan | Secreted Insecticidal Protein and Gene Compositions from Bacillus Thuringiensis and Uses Therefor |
| EP1818405B1 (en) | 2004-04-09 | 2015-06-03 | Monsanto Technology, LLC | Compositions and methods for control of insect infestations in plants |
| DE602007004340D1 (de) * | 2006-07-21 | 2010-03-04 | Du Pont | Verfahren zur identifizierung neuer gene |
| WO2009036234A1 (en) * | 2007-09-14 | 2009-03-19 | Athenix Corporation | Synthetic axmi-004 delta-endotoxin genes and methods for their use |
| BRPI0821964A2 (pt) * | 2008-01-10 | 2014-10-21 | Pioneer Hi Bred Int | Molécula de ácido nucleico isolado, construção de dna, polipeptídeo isolado com atividade pesticida, composição, método para controlar uma praga de população de coleópteros, método para matar uma praga de coleópteros, método para produzir um polipeptídeo com atividade pesticida, planta de uma construção de dna estavelmente incorporado ao seu genoma e método para proteger uma planta de uma praga |
| US20100077507A1 (en) * | 2008-09-22 | 2010-03-25 | Pioneer Hi-Bred International, Inc. | Novel Bacillus Thuringiensis Gene with Lepidopteran Activity |
| AR075114A1 (es) * | 2008-12-23 | 2011-03-09 | Athenix Corp | Gen de delta-endotoxina axmi-150 y metodos de uso del mismo |
| AR075634A1 (es) * | 2009-02-27 | 2011-04-20 | Athenix Corp | Proteinas pesticidas y metodos de uso de las mismas |
| US8586832B2 (en) * | 2009-12-21 | 2013-11-19 | Pioneer Hi Bred International Inc | Bacillus thuringiensis gene with Lepidopteran activity |
| CN102382876A (zh) * | 2010-08-30 | 2012-03-21 | 高希武 | 一种检测小菜蛾对阿维菌素类农药抗药性的方法 |
| CN103596436B (zh) * | 2011-04-07 | 2020-03-06 | 孟山都技术公司 | 具有对抗半翅目和/或鳞翅目昆虫的活性的昆虫抑制毒素家族 |
| US11692016B2 (en) | 2012-03-09 | 2023-07-04 | Vestaron Corporation | High gene expression yeast strain |
| KR20240116574A (ko) | 2012-03-09 | 2024-07-29 | 베스타론 코포레이션 | 독성 펩타이드 제조, 식물에서의 펩타이드 발현 및 시스테인 농후 펩타이드의 조합 |
| AP2016009601A0 (en) * | 2014-06-20 | 2016-12-31 | Dow Agrosciences Llc | Vegetative insecticidal proteins useful for control of insect pests |
| BR112017007932A2 (pt) * | 2014-10-16 | 2018-01-23 | Du Pont | proteínas inseticidas e métodos para uso das mesmas |
| ES2904255T3 (es) * | 2015-07-30 | 2022-04-04 | Monsanto Technology Llc | Proteínas inhibidoras de insectos novedosas |
| US10055521B2 (en) * | 2016-04-15 | 2018-08-21 | International Business Machines Corporation | Fly-height interaction simulation |
| CR20240410A (es) * | 2016-10-10 | 2024-11-08 | Monsanto Technology Llc | PROTEÍNAS INHIBIDORAS DE INSECTOS NOVEDOSAS (Divisional Exp. 2019-0221) |
| AU2017347761C1 (en) | 2016-10-21 | 2024-03-28 | Vestaron Corporation | Cleavable peptides and insecticidal and nematicidal proteins comprising same |
| CN109055251B (zh) * | 2017-10-31 | 2021-08-10 | 上海大学 | 一种芽孢杆菌及其应用 |
| CN111909950B (zh) * | 2019-05-09 | 2022-09-23 | 中国科学院分子植物科学卓越创新中心 | 用于提高刺糖孢菌中多杀菌素产量的基因及其应用 |
| WO2022016021A1 (en) | 2020-07-15 | 2022-01-20 | Third Harmonic Bio, Inc. | Crystalline forms of a selective c-kit kinase inhibitor |
| EP4247368A4 (en) | 2020-11-19 | 2024-10-23 | Third Harmonic Bio, Inc. | PHARMACEUTICAL COMPOSITIONS OF A SELECTIVE C-KIT KINASE INHIBITOR AND METHODS FOR THE PREPARATION AND USE THEREOF |
| CN112480220B (zh) * | 2020-12-02 | 2021-10-12 | 东北农业大学 | 苏云金芽胞杆菌Sip1Aa蛋白随机重组突变蛋白 |
| CN112608866B (zh) * | 2020-12-22 | 2022-12-06 | 四川大学 | 巨大芽孢杆菌及其活化方法、泡菜制作方法及泡菜 |
| CN114431179A (zh) * | 2022-02-25 | 2022-05-06 | 生态环境部南京环境科学研究所 | 一种Bt杀虫蛋白对大型蚤安全性影响的评价方法 |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4554101A (en) | 1981-01-09 | 1985-11-19 | New York Blood Center, Inc. | Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity |
| GB8425487D0 (en) | 1984-10-09 | 1984-11-14 | Agricultural Genetics Co | Strain of bacillus thuringiensis |
| US4695462A (en) | 1985-06-28 | 1987-09-22 | Mycogen Corporation | Cellular encapsulation of biological pesticides |
| US4695455A (en) | 1985-01-22 | 1987-09-22 | Mycogen Corporation | Cellular encapsulation of pesticides produced by expression of heterologous genes |
| US5080897A (en) | 1987-05-08 | 1992-01-14 | Ecogen Inc. | Novel bacillus thuringiensis strains, and related insecticidal compositions |
| ES2077589T3 (es) | 1988-04-25 | 1995-12-01 | Monsanto Co | Plantas de lechuga resistentes a insectos. |
| US5416011A (en) | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
| US5135867A (en) | 1988-11-01 | 1992-08-04 | Mycogen Corporation | Gene encoding a lepidopteran-active toxin from Bacillus thuringiensis isolate denoted B.t. .PS81GG active against lepidopteran pests |
| JP3364616B2 (ja) | 1989-02-24 | 2003-01-08 | モンサント テクノロジー エルエルシー | 合成植物遺伝子と調製方法 |
| US5593874A (en) | 1992-03-19 | 1997-01-14 | Monsanto Company | Enhanced expression in plants |
| US6156308A (en) * | 1992-12-15 | 2000-12-05 | Valent Biosciences, Inc. | Bacillus thuringiensis strains active against lepidopteran and coleopteran pests |
| US5849870A (en) | 1993-03-25 | 1998-12-15 | Novartis Finance Corporation | Pesticidal proteins and strains |
| CN1119877A (zh) | 1993-03-25 | 1996-04-03 | 希巴-盖吉股份公司 | 新杀虫蛋白和菌株 |
| US5689052A (en) | 1993-12-22 | 1997-11-18 | Monsanto Company | Synthetic DNA sequences having enhanced expression in monocotyledonous plants and method for preparation thereof |
| CN1037913C (zh) | 1995-12-28 | 1998-04-01 | 中国农业科学院生物技术研究中心 | 编码杀虫蛋白质的融合基因和表达载体及其应用 |
| US6063756A (en) | 1996-09-24 | 2000-05-16 | Monsanto Company | Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor |
| US5942664A (en) * | 1996-11-27 | 1999-08-24 | Ecogen, Inc. | Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants |
| AU2001259260A1 (en) | 2000-05-15 | 2001-11-26 | Monsanto Technology Llc | Polypeptide composionns toxic to anthonomus insects, and use thereof |
| AR030718A1 (es) * | 2000-09-12 | 2003-09-03 | Monsanto Technology Llc | Proteinas de bacillus thuringiensis inhibidoras de insectos fusiones y metodos de uso de las mismas |
| US7355099B2 (en) * | 2003-02-20 | 2008-04-08 | Athenix Corporation | AXMI-004, a delta-endotoxin gene and methods for its use |
| US20040197917A1 (en) * | 2003-02-20 | 2004-10-07 | Athenix Corporation | AXMI-014, delta-endotoxin gene and methods for its use |
| EP1947184B1 (en) * | 2003-02-20 | 2011-03-30 | Athenix Corporation | Delta-endotoxin genes and methods for their use |
| EP1644480B1 (en) | 2003-07-07 | 2013-06-05 | Monsanto Technology, LLC | Insecticidal proteins secreted from bacillus thuringiensis and uses therefor |
| US20080040827A1 (en) | 2003-12-16 | 2008-02-14 | Judith Donovan | Secreted Insecticidal Protein and Gene Compositions from Bacillus Thuringiensis and Uses Therefor |
-
2004
- 2004-12-14 US US10/581,763 patent/US20080040827A1/en not_active Abandoned
- 2004-12-14 EP EP04821812A patent/EP1708561B1/en not_active Expired - Lifetime
- 2004-12-14 AT AT04821812T patent/ATE487372T1/de active
- 2004-12-14 CN CNA2004800377074A patent/CN101133079A/zh active Pending
- 2004-12-14 BR BRPI0417735-5A patent/BRPI0417735A/pt not_active Application Discontinuation
- 2004-12-14 CA CA2547933A patent/CA2547933C/en not_active Expired - Fee Related
- 2004-12-14 WO PCT/US2004/042611 patent/WO2005107383A2/en active Application Filing
- 2004-12-14 ES ES04821812T patent/ES2353603T3/es not_active Expired - Lifetime
- 2004-12-14 MX MXPA06007005A patent/MXPA06007005A/es active IP Right Grant
- 2004-12-14 DE DE602004030044T patent/DE602004030044D1/de not_active Expired - Lifetime
- 2004-12-16 AR ARP040104700A patent/AR046944A1/es not_active Application Discontinuation
-
2006
- 2006-06-07 ZA ZA200604678A patent/ZA200604678B/xx unknown
-
2011
- 2011-06-29 US US13/171,519 patent/US8569583B2/en not_active Expired - Lifetime
-
2012
- 2012-11-21 US US13/682,774 patent/US20130212748A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20080040827A1 (en) | 2008-02-14 |
| BRPI0417735A (pt) | 2007-04-03 |
| CA2547933C (en) | 2014-08-12 |
| CA2547933A1 (en) | 2005-11-17 |
| WO2005107383A3 (en) | 2007-04-12 |
| EP1708561B1 (en) | 2010-11-10 |
| US8569583B2 (en) | 2013-10-29 |
| DE602004030044D1 (de) | 2010-12-23 |
| EP1708561A2 (en) | 2006-10-11 |
| MXPA06007005A (es) | 2008-02-14 |
| ATE487372T1 (de) | 2010-11-15 |
| WO2005107383A2 (en) | 2005-11-17 |
| EP1708561A4 (en) | 2008-03-05 |
| US20130212748A1 (en) | 2013-08-15 |
| ES2353603T3 (es) | 2011-03-03 |
| WO2005107383A8 (en) | 2007-05-24 |
| CN101133079A (zh) | 2008-02-27 |
| ZA200604678B (en) | 2008-06-25 |
| US20110283417A1 (en) | 2011-11-17 |
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Legal Events
| Date | Code | Title | Description |
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| FC | Refusal |