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AR032284A1 - NUCLEAR TRANSFER WITH SELECTED DONOR CELLS - Google Patents

NUCLEAR TRANSFER WITH SELECTED DONOR CELLS

Info

Publication number
AR032284A1
AR032284A1 ARP000104816A ARP000104816A AR032284A1 AR 032284 A1 AR032284 A1 AR 032284A1 AR P000104816 A ARP000104816 A AR P000104816A AR P000104816 A ARP000104816 A AR P000104816A AR 032284 A1 AR032284 A1 AR 032284A1
Authority
AR
Argentina
Prior art keywords
cells
reconstituted
cell
nutraceutical
pharmaceutical
Prior art date
Application number
ARP000104816A
Other languages
Spanish (es)
Inventor
David Wells
Original Assignee
Agres Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agres Ltd filed Critical Agres Ltd
Publication of AR032284A1 publication Critical patent/AR032284A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/99—Coculture with; Conditioned medium produced by genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Neurology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Diabetes (AREA)
  • General Engineering & Computer Science (AREA)
  • Neurosurgery (AREA)
  • Microbiology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Hematology (AREA)
  • Cardiology (AREA)
  • Endocrinology (AREA)
  • Cell Biology (AREA)
  • Obesity (AREA)
  • Reproductive Health (AREA)
  • Oncology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Psychology (AREA)
  • Emergency Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Orthopedic Medicine & Surgery (AREA)

Abstract

Un método de transferencia nuclear, caracterizado porque comprende la seleccion y segregacion de células G1 de una poblacion proliferativa o no proliferativa de células donantes y la transferencia de un nucleo de dicha célula G1 segregada una célula receptora enucleada. Este método es ventajoso en comparacion con el arte previo debido a que provee certeza en cuanto a la etapa del ciclo celular en que se encuentran los nucleos donantes y permite la produccion de células embrionarias transgénicas o no transgénicas, embriones reconstituidos y animales completos para aplicaciones agrícolas, farmacéuticas, nutracéuticas y biomédicas. También se divulga un embrion animal reconstituido, un animal no humano clonado, progenie y descendientes de este ultimo, una línea celular embrionaria y uso de esta ultima, métodos para producir la misma y métodos de clonacion terapéutica para tratamiento de una enfermedad, para el descubrimiento de drogas, de xenotransplante y de terapia genética reconstituidos y animales completos para aplicaciones agrícolas, farmacéuticas, nutracéuticas y biomédicas.A method of nuclear transfer, characterized in that it comprises the selection and segregation of G1 cells from a proliferative or non-proliferative population of donor cells and the transfer of a nucleus from said segregated G1 cell to an enucleated receptor cell. This method is advantageous compared to the prior art because it provides certainty as to the stage of the cell cycle in which the donor nuclei are found and allows the production of transgenic or non-transgenic embryonic cells, reconstituted embryos and complete animals for agricultural applications. , pharmaceutical, nutraceutical and biomedical. A reconstituted animal embryo, a cloned non-human animal, progeny and descendants of the latter, an embryonic cell line and use of the latter, methods to produce the same and therapeutic cloning methods for treatment of a disease, for the discovery, are also disclosed. of reconstituted drugs, xenotransplantation and gene therapy and complete animals for agricultural, pharmaceutical, nutraceutical and biomedical applications.

ARP000104816A 1999-09-14 2000-09-14 NUCLEAR TRANSFER WITH SELECTED DONOR CELLS AR032284A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NZ337792A NZ337792A (en) 1999-09-14 1999-09-14 Nuclear transfer and use in cloning

Publications (1)

Publication Number Publication Date
AR032284A1 true AR032284A1 (en) 2003-11-05

Family

ID=19927507

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP000104816A AR032284A1 (en) 1999-09-14 2000-09-14 NUCLEAR TRANSFER WITH SELECTED DONOR CELLS

Country Status (8)

Country Link
JP (1) JP2004500038A (en)
CN (1) CN1424870A (en)
AR (1) AR032284A1 (en)
AU (1) AU784371B2 (en)
DE (1) DE10084987T1 (en)
GB (1) GB2369828B (en)
NZ (1) NZ337792A (en)
WO (1) WO2001019182A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2272482T3 (en) 2000-01-20 2007-05-01 Diabcell Pty Limited PREPARATION AND XENOTRANSPLANT OF SWINE ISLOTS.
CN1735338A (en) * 2002-12-10 2006-02-15 Gtc生物治疗学公司 Methods and systems for additional nuclear transfer using somatic cell nuclear transfer embryos as cell donors
JP2006081542A (en) * 2004-08-18 2006-03-30 Institute Of Physical & Chemical Research How to make a cloned mammal
JP2010075099A (en) * 2008-09-26 2010-04-08 Japan Science & Technology Agency Method for screening mammal nuclear transplantation embryo, nonhuman mammal nuclear transplantation embryo, clone nonhuman mammal and screening kit
US10570418B2 (en) 2014-09-02 2020-02-25 The Regents Of The University Of California Methods and compositions for RNA-directed target DNA modification
CN105483216B (en) * 2015-11-30 2019-01-15 四川农业大学 A kind of method of rabbit CETP genetic test meat breeding
CN108624621B (en) * 2018-01-17 2019-04-12 中国科学院上海生命科学研究院 The preparation method of the somatic cell clone animal of non-human primates
MX2022011245A (en) 2020-03-11 2023-01-11 Bit Bio Ltd Method of generating hepatic cells.
CN111793671A (en) * 2020-07-22 2020-10-20 中国农业大学 An optimized method for preparing chromosome suspension suitable for binary flow sorting of sheep chromosomes
CN112715479A (en) * 2021-01-06 2021-04-30 吉沙阿牛 Goat ecological cycle breeding method

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999046982A1 (en) * 1998-03-16 1999-09-23 Relag Pty Ltd Porcine nuclear transfer

Also Published As

Publication number Publication date
CN1424870A (en) 2003-06-18
WO2001019182A1 (en) 2001-03-22
GB0205915D0 (en) 2002-04-24
NZ337792A (en) 2002-03-28
AU784371B2 (en) 2006-03-23
GB2369828A (en) 2002-06-12
AU7462400A (en) 2001-04-17
GB2369828B (en) 2004-12-01
DE10084987T1 (en) 2003-04-17
JP2004500038A (en) 2004-01-08

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