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AR016223A1 - Fragmento aislado de acidos nucleicos que codifica enzimas de la biosintesis de carotenoides, gen quimerico, celula huesped transformada, polipeptido,metodos para alterar el nivel de expresion de dichas enzimas, metodos para obtener dicho fragmento, productos que se obtienen por dichos metodos y met - Google Patents

Fragmento aislado de acidos nucleicos que codifica enzimas de la biosintesis de carotenoides, gen quimerico, celula huesped transformada, polipeptido,metodos para alterar el nivel de expresion de dichas enzimas, metodos para obtener dicho fragmento, productos que se obtienen por dichos metodos y met

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Publication number
AR016223A1
AR016223A1 ARP990101902A ARP990101902A AR016223A1 AR 016223 A1 AR016223 A1 AR 016223A1 AR P990101902 A ARP990101902 A AR P990101902A AR P990101902 A ARP990101902 A AR P990101902A AR 016223 A1 AR016223 A1 AR 016223A1
Authority
AR
Argentina
Prior art keywords
methods
enzymes
expression
nucleic acid
level
Prior art date
Application number
ARP990101902A
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English (en)
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of AR016223A1 publication Critical patent/AR016223A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se revelan fragmentos de ácidos nucleicos aislados que codifican enzimas biosintéticas de carotenoides, más especifícamente con un fragmento aislado deácidos nucleicos que codifica una fitoeno sintetasa o una zeaxantina epoxidasa y su fragmento complementario. Una realizacion adicional se relaciona con unpolipéptido que codifica una enzima biosintética de carotenoides seleccionada del grupo formado por fitoeno sintetasa o zeaxantina epoxidasa. En otrarealizacion, se relaciona con un gen quimérico que comprende un fragmento de ácidos nucleicos que codifica un fitoenosintetasa o una zeaxantina epoxidasa,ligado operativamente a secuencias reguladoras adecuadas, donde la expresion del gen quimérico da como resultado la produccion de niveles alterados (es decir,incrementados o disminuidos) de la proteína codificada en una célula huésped transformada, que también se describe, con respecto a los niveles producidos enuna célula huésped no transformada. En realizaciones adicionales se refierenun método para alterar el nivel de expresion de una fitoeno sintetasa o unazeaxantina epoxidasa en una célula huésped transformada, un método para obtener dicho fragmento de ácido nucleico, los productos que se obtienen. Asimismo sedescribe un métodopara evaluar por lo menos un compuesto por su capacidad de inhibir la actividad de dichas enzimas.
ARP990101902A 1998-04-24 1999-04-23 Fragmento aislado de acidos nucleicos que codifica enzimas de la biosintesis de carotenoides, gen quimerico, celula huesped transformada, polipeptido,metodos para alterar el nivel de expresion de dichas enzimas, metodos para obtener dicho fragmento, productos que se obtienen por dichos metodos y met AR016223A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US8304298P 1998-04-24 1998-04-24

Publications (1)

Publication Number Publication Date
AR016223A1 true AR016223A1 (es) 2001-06-20

Family

ID=22175804

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP990101854A AR016216A1 (es) 1998-04-24 1999-04-22 Fragmento aislado de acidos nucleicos que codifica un polipeptido de por lo menos 40 aminoacidos, que tiene al menos un 80% de identidad, basado en elmetodo clustal de alineamiento, con una enzima de la biosintesis de carotenoides en semillas y plantas, gen quimerico, celula huesped transformada, po
ARP990101902A AR016223A1 (es) 1998-04-24 1999-04-23 Fragmento aislado de acidos nucleicos que codifica enzimas de la biosintesis de carotenoides, gen quimerico, celula huesped transformada, polipeptido,metodos para alterar el nivel de expresion de dichas enzimas, metodos para obtener dicho fragmento, productos que se obtienen por dichos metodos y met

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP990101854A AR016216A1 (es) 1998-04-24 1999-04-22 Fragmento aislado de acidos nucleicos que codifica un polipeptido de por lo menos 40 aminoacidos, que tiene al menos un 80% de identidad, basado en elmetodo clustal de alineamiento, con una enzima de la biosintesis de carotenoides en semillas y plantas, gen quimerico, celula huesped transformada, po

Country Status (6)

Country Link
US (1) US6627795B1 (es)
EP (3) EP1071800A2 (es)
AR (2) AR016216A1 (es)
AU (3) AU3749199A (es)
BR (1) BR9910038A (es)
WO (3) WO1999055887A2 (es)

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US6429356B1 (en) 1996-08-09 2002-08-06 Calgene Llc Methods for producing carotenoid compounds, and specialty oils in plant seeds
US6653530B1 (en) 1998-02-13 2003-11-25 Calgene Llc Methods for producing carotenoid compounds, tocopherol compounds, and specialty oils in plant seeds
CA2369844C (en) 1999-04-15 2013-02-26 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
AU2001240069A1 (en) * 2000-03-07 2001-09-17 Cargill Incorporated Production of lutein in microorganisms
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
DE10022362A1 (de) * 2000-05-08 2001-11-15 Bayer Ag Verfahren zum Auffinden von Modulatoren von Enzymen des Carotenoid-Biosyntheseweges
AR028450A1 (es) * 2000-05-12 2003-05-07 Monsanto Technology Llc Mctodos para producir compuestos carotenoides y aceites especiales en semillas de plantas
AR030124A1 (es) 2000-08-07 2003-08-13 Monsanto Technology Llc Genes de la via del fosfato de metil-d-eritritol
BR0209483A (pt) 2001-05-09 2004-07-06 Monsanto Technology Llc Genes tyra e seus usos
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
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AU2002329759A1 (en) 2001-08-17 2003-03-03 Monsanto Technology Llc Methyltransferase genes and uses thereof
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CN113549639B (zh) * 2021-07-21 2022-07-29 云南中烟工业有限责任公司 一种降低烟叶总蛋白及烟气苯酚含量的调控基因

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Also Published As

Publication number Publication date
BR9910038A (pt) 2001-10-09
WO1999055888A2 (en) 1999-11-04
WO1999055887A3 (en) 2000-04-13
AR016216A1 (es) 2001-06-20
AU3754499A (en) 1999-11-16
WO1999055889A2 (en) 1999-11-04
AU3749199A (en) 1999-11-16
WO1999055888A3 (en) 2000-04-13
WO1999055889A3 (en) 2000-04-06
US6627795B1 (en) 2003-09-30
EP1071801A2 (en) 2001-01-31
AU3864999A (en) 1999-11-16
EP1071800A2 (en) 2001-01-31
WO1999055887A2 (en) 1999-11-04
EP1071802A2 (en) 2001-01-31

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