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AR014351A1 - Fragmento aislado de acidos nucleicos que codifica enzimas de la biosintesis de aminoacidos en plantas, gen quimerico, celula huespedtransformada, polipeptido, metodos para alterar el nivel de expresion de dichas enzimas, metodos para obtener y seleccionar dicho fragmento, productos que se - Google Patents

Fragmento aislado de acidos nucleicos que codifica enzimas de la biosintesis de aminoacidos en plantas, gen quimerico, celula huespedtransformada, polipeptido, metodos para alterar el nivel de expresion de dichas enzimas, metodos para obtener y seleccionar dicho fragmento, productos que se

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Publication number
AR014351A1
AR014351A1 ARP980102658A ARP980102658A AR014351A1 AR 014351 A1 AR014351 A1 AR 014351A1 AR P980102658 A ARP980102658 A AR P980102658A AR P980102658 A ARP980102658 A AR P980102658A AR 014351 A1 AR014351 A1 AR 014351A1
Authority
AR
Argentina
Prior art keywords
enzymes
methods
nucleic acid
biosynthesis
amino acids
Prior art date
Application number
ARP980102658A
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English (en)
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of AR014351A1 publication Critical patent/AR014351A1/es

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se revela un fragmento aislado de ácidos nucleicos que codifica una enzima vegetal que cataliza pasos en la biosintesis de los aminoácidos lisina,treonina, metionina, cisteína e isoleucina a partir de aspartato, donde dicha enzima es un miembro del grupo formado por: dihidrodipicolinato reductasa,diaminpimelato epimerasa, treonina sintetasa, treonina deaminasa y S-adenosilmetionina sintetasa. También se refieren genes quiméricos quecodifican las enzimas biosintéticas de aminoácidos enumeradas anteriormente o genes quiméricos que comprenden fragmentos de ácidos nucleicos que soncomplementarios de los fragmentos de ácidos nucleicos que codifican dichas enzimas, ligados operativamente con secuencias reguladoras adecuadasy celulastransformadas. Se refiere también un método para alterar el nivel de expresion de una enzima biosintética vegetal en una célula huésped transformada. Serevelan asimismo, un método para obtener y seleccionar dicho fragmento de ácido nucleicoy un método para evaluar por lo menos un compuesto por su capacidadpara inhibir la actividad de una de las enzimas biosintéticas vegetales anteriormente divulgadas.
ARP980102658A 1997-06-06 1998-06-05 Fragmento aislado de acidos nucleicos que codifica enzimas de la biosintesis de aminoacidos en plantas, gen quimerico, celula huespedtransformada, polipeptido, metodos para alterar el nivel de expresion de dichas enzimas, metodos para obtener y seleccionar dicho fragmento, productos que se AR014351A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4877197P 1997-06-06 1997-06-06
US4944397P 1997-06-12 1997-06-12

Publications (1)

Publication Number Publication Date
AR014351A1 true AR014351A1 (es) 2001-02-28

Family

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Family Applications (1)

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ARP980102658A AR014351A1 (es) 1997-06-06 1998-06-05 Fragmento aislado de acidos nucleicos que codifica enzimas de la biosintesis de aminoacidos en plantas, gen quimerico, celula huespedtransformada, polipeptido, metodos para alterar el nivel de expresion de dichas enzimas, metodos para obtener y seleccionar dicho fragmento, productos que se

Country Status (8)

Country Link
US (2) US6664445B1 (es)
EP (2) EP0979296A2 (es)
CN (1) CN1259170A (es)
AR (1) AR014351A1 (es)
BR (1) BR9809967A (es)
CA (1) CA2292778A1 (es)
ID (1) ID24686A (es)
WO (1) WO1998055601A2 (es)

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ID24686A (id) 2000-07-27
EP1959016A2 (en) 2008-08-20
US7022895B2 (en) 2006-04-04
EP1959016A3 (en) 2008-11-05
CA2292778A1 (en) 1998-12-10
CN1259170A (zh) 2000-07-05
US6664445B1 (en) 2003-12-16
WO1998055601A3 (en) 1999-03-04
WO1998055601A2 (en) 1998-12-10
US20040209341A1 (en) 2004-10-21
EP0979296A2 (en) 2000-02-16
BR9809967A (pt) 2000-08-01

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