Eman A Elghoroury
National Research Centre, Clinical Pathology, Faculty Member
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Background Breast cancer (BC) is the most common cancer among Egyptian women, and its early detection is accompanied with decreased mortality rates. MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate gene expression and... more
Background Breast cancer (BC) is the most common cancer among Egyptian women, and its early detection is accompanied with decreased mortality rates. MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate gene expression and affect cancer development. The expression level of miRNA-155 is up-regulated in BC and correlates with its invasive properties, offering a great potential as a noninvasive biomarker for BC screening. Objective The aim of this study is to evaluate the role of miRNA-155 as an early diagnostic serum-based biomarker of BC. Patients and methods This study was conducted on 30 patients newly diagnosed with BC and associated 20 healthy women as a control group. Serum level of miRNA-155 was measured for all studied cases using real-time quantitative PCR technique. Results MiRNA-155 expression was up-regulated in serum of patients with BC compared with control group (P<0.001). Serum miRNA-155 expression significantly differed with cancer stage and its expression directly increased with advancing cancer stage. A comparative study of the miRNA-155 expression between control group and positive and negative metastatic lymph nodes (LNs) subgroups demonstrates a highly significant difference. MiRNA-155, in terms of fold change, is highest in positive LNs metastatic subgroup, with a mean of 19.63, followed by 4.29 in negative LNs metastasis subgroup, and lowest in the control group, with a mean of 1.06 (P<0.001). Conclusion The results validate miRNA-155 as a promising diagnostic and even a prognostic marker in cases of BC.
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s 19th International Congress of Nutrition October 4–9, 2009, Bangkok, Thailand Editors: Tontisirin, K.; Wasantwisut, E. (Bangkok)
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Research Interests: Hematology, Gastroenterology, Biology, Egypt, Adolescent, and 15 moreGene expression, Humans, Child, Internal Medicine, Female, Ferritin, Clinical Sciences, Genotype, Genotyping, Genetic Polymorphism, Blood Transfusion, Glutathione Transferase, Beta Thalassemia, Case Control Studies, and Cardiovascular medicine and haematology
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Background/aim Iron overload is the main concern in treatment of hemolytic diseases with repeated blood transfusion, especially sickle cell disease (SCD). Hepcidin has appeared as the key iron metabolism regulator. Erythroferrone (ERFE)... more
Background/aim Iron overload is the main concern in treatment of hemolytic diseases with repeated blood transfusion, especially sickle cell disease (SCD). Hepcidin has appeared as the key iron metabolism regulator. Erythroferrone (ERFE) is postulated to function as the chief erythroid regulator. Transferrin receptor 2 (TfR2) acts as an iron sensor on erythroid cells. Our aim is to evaluate serum levels of hepcidin, ferritin, ERFE, and TfR2 and its correlation with molecular genetic study of hepcidin gene expression for SCD patients Patients and methods Patients: 103 children aged 6–18 years with SCD were recruited from the Pediatric Hematology Clinic at the National Research Center and Abo-Elrish Hospital (Cairo University), and 55 healthy children with matched age and sex served as the control group. Methods: laboratory analysis and enzyme-linked immunosorbent assay tests on patient samples were performed for serum hepcidin, ERFE, ferritin and TfR2, and hepcidin gene expression was performed by quantitative real-time PCR. Results Hepcidin RNA expression level showed significant correlation with the duration of the disease and blood transfusion frequency (r=−0.33, P<0.001) (r=−0.270, P 0.006), respectively. Conclusion Combination of enzyme-linked immunosorbent assay and molecular studies of hepcidin RNA expression could be a diagnostic marker to be used in conjunction with analytical techniques to detect iron overload in pediatric sickle cell disease.
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BACKGROUND Blood transfusion (BT) is essential in treating sickle cell disease (SCD); however, it leads to iron overload (IO) and oxidative stress. We studied the relationship between oxidative stress, iron status parameters, hepcidin... more
BACKGROUND Blood transfusion (BT) is essential in treating sickle cell disease (SCD); however, it leads to iron overload (IO) and oxidative stress. We studied the relationship between oxidative stress, iron status parameters, hepcidin mRNA gene expression, and IO in SCD patients. METHODS We classified all SCD patients (n = 90) into two groups: Group I, 45 children (s.ferritin ≥ 938 ng/mL) and Group II, 45 children (s.ferritin < 938 ng/mL). A total of 55 children, age and sex matched, participated as a control group. Malondialdehyde (MDA), nitrite, s.iron, s.total iron-binding capacity (sTIBC), transferrin saturation %, s.ferritin, s.hepcidin, and hepcidin mRNA gene expression were assessed. RESULTS Among SCD BT-dependent patients (>3 times/year), 63% were from Group I and 37% from Group II, p < .01. The two patient groups had significantly lower s.hepcidin and hepcidin gene expression than controls (p < .001). TIBC, s.iron, s.ferritin, transferrin saturation %, ferritin/hepcidin ratio, and MDA levels were higher among SCD patients than controls (p < .001). Group I had higher mean level of ferritin/hepcidin ratio and MDA than Group II (p < .01). The higher level of MDA and increased frequency of BT were the significant predicting risk factors for IO (p < .05). A receiver-operating characteristic curve indicates that MDA is the outstanding significant biomarker for high level of s.ferritin with subsequent IO progression. CONCLUSION MDA may serve as a biomarker of oxidative stress and IO in SCD patients. This result paid attention for urgent initiation of antioxidant and chelation therapy on detecting increased MDA level.
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Introduction: Regulatory T cells (Treg) are emerging as a potential therapy to facilitate long-term allograft survival what makes identification of reliable surface markers that are selectively expressed on Treg is crucial. The aim of... more
Introduction: Regulatory T cells (Treg) are emerging as a potential therapy to facilitate long-term allograft survival what makes identification of reliable surface markers that are selectively expressed on Treg is crucial. The aim of this study is to evaluate the regulatory and suppressive functions of CD127 in peripheral T lymphocytes of pediatric kidney transplant recipients through studying the association their frequency and the development of rejection. Material and methods: Flow cytometric analysis of peripheral blood samples for the CD127 surface marker of 50 pediatric transplant recipients and 12 healthy controls was done. Clinical, laboratory, immunosuppressive therapy data and graft function of transplant recipients were collected and correlated with their CD127 peripheral blood expression. Results: CD127 expression in transplanted children was significantly elevated than that of controls (2.76 ±3.26% vs. 0.95 ±0.94%, p = 0.042). CD 127 expression did not correlate with d...
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Background Hepatocellular carcinoma (HCC) is known to be the second leading cause of cancer-related mortality worldwide. For improving the prognosis as well as reducing the rate of mortality, early diagnosis of HCC is a must. Aims This... more
Background Hepatocellular carcinoma (HCC) is known to be the second leading cause of cancer-related mortality worldwide. For improving the prognosis as well as reducing the rate of mortality, early diagnosis of HCC is a must. Aims This study was conducted to assess the ability of the serum expression of exosomal miR-18a and miR-222 to differentiate and diagnose patients with HCC, patients with liver cirrhosis, and healthy controls. Methods This study included 51 patients with liver cirrhosis, 51 patients with HCC on top of hepatitis C virus (HCV) infection, and 50 healthy controls. Results miR-18a and miR-222 were assessed using reverse transcription-polymerase chain reaction. MiR-18a and miR-222 levels were significantly higher in the liver cirrhosis and HCC groups than the control group ( p ˂ 0.001). However, no statistically significant difference was found between patients with HCC and liver cirrhosis ( p = 0.4 for miR-18a and p = 0.1 for miR-222). ROC curve analyses to evaluate...
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The role of CD4+CD25+ T regulatory cells (Tregs) in immune tolerance in experimental transplantation is very important but the clinical significance of circulating Tregs in the peripheral blood is undetermined. We evaluated the... more
The role of CD4+CD25+ T regulatory cells (Tregs) in immune tolerance in experimental transplantation is very important but the clinical significance of circulating Tregs in the peripheral blood is undetermined. We evaluated the association between the frequency of T cell activation markers CD25 and CD71 and clinical parameters that may affect the level of these T cell markers. In 47peditric kidney transplant (KT) recipients and 20 healthy controls, the frequency of T cell activation markers, CD25 and CD71 was measured with flow cytometry after transplantation. Two clinical protocols of induction immunosuppression were used: (1) anti-thymocyte globulin (THYMO) group (n =29) and Basiliximab (BSX) group (n=10). The percentage of circulating CD25 after KT was significantly lower than that in the controls. There is no significant difference between KT and the controls s regard to circulating CD71. The percentage of CD25 was significantly increased in children with acute rejection compare...
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The high prevalence of protein-energy malnutrition is a critical issue for patients with chronic kidney disease (CKD). Serum albumin is the most commonly used nutritional marker. Another index is plasma amino acid (AA) profile. Of these,... more
The high prevalence of protein-energy malnutrition is a critical issue for patients with chronic kidney disease (CKD). Serum albumin is the most commonly used nutritional marker. Another index is plasma amino acid (AA) profile. Of these, the plasma levels of glutamine, glutamate and homocysteine, correlate well with nutritional status. We measured some plasma AAs in children with different stages CKD to provide information in monitoring the therapeutic strategy, particularly in AA supplementary therapy or protein restriction. Three amino acids were evaluated along with albumin and high sensitivity C-reactive protein (hs-CRP) in 30 patients with advanced CKD stages 4 and 5. They were divided into two groups undergoing conservative treatment (CT) (n=15) or hemodialysis (HD) (n=15). An additional group of patients with nephrotic syndrome [CKD stage 2] was also studied to assess the alterations of plasma free amino acids with the early stage of CKD. Another 30 age- and sex-matched healt...
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Chemokine receptor 2 (CCR2) may have an impact on end stage renal disease (ESRD) development in children as well as renal allograft survival. Objective: Detection of the relevance of the CCR2 V64I gene polymorphism to the development and... more
Chemokine receptor 2 (CCR2) may have an impact on end stage renal disease (ESRD) development in children as well as renal allograft survival. Objective: Detection of the relevance of the CCR2 V64I gene polymorphism to the development and progression of ESRD and its impact on graft rejection in transplanted children. Methods: Genotyping for CCR2 V64I was done for seventy five children with end-stage renal disease (ESRD) [50 treated with renal transplantation and 25 with hemodialysis] and seventy five healthy children by polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP) analysis. Results: The CCR2 V64I displayed significantly higher frequencies among transplantation, hemodialysis, ESRD-patients as well as those with acute rejection when compared with the control subjects (P value <0.001for all).The mutant A allele displayed statistically significant frequencies in all groups when compared with the control group (P value < 0.001). Moreover, carriers of...
Background and objectives: Data concerning the concentration of matrix metalloproteinase-9 (MMP-9) and its functional polymorphisms in chronic kidney diseases (CKD) are conflicting. The present study aimed to evaluate the levels of... more
Background and objectives: Data concerning the concentration of matrix metalloproteinase-9 (MMP-9) and its functional polymorphisms in chronic kidney diseases (CKD) are conflicting. The present study aimed to evaluate the levels of MMP-9in children with end stage renal diseases (ESRD) on hemodialysis (HD) and to explore its association with MMP-9 polymorphism and vitamin D levels as an important risk factors for cardiovascular diseases (CVD). Methods: We studied 55 children with ESRD on hemodialysis and 18 healthy children served as controls. MMP-9 and vitamin D levels were measured by ELISA in serum of all patients and controls. Genotypes for MMP-9 polymorphism(C-1562T) were determined by RFLP for only 28 of the patients and all the controls. Results: There were insignificantly reduced MMP-9levels of patients vs. controls, however, there was significant increase in MMP-9 levels associated with CC genotypes for(C-1562T) polymorphism compared with CT genotype (p=0.01). We found that ...
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There is an urgent need to develop alternative to kidney biopsy in children to diagnose nephrotic syndrome. Increased apoptosis plays a central role in the development of nephrotic syndrome. The aim of this study was to evaluate the blood... more
There is an urgent need to develop alternative to kidney biopsy in children to diagnose nephrotic syndrome. Increased apoptosis plays a central role in the development of nephrotic syndrome. The aim of this study was to evaluate the blood levels of two markers of apoptosis, Fas-ligand (Fas-l) and granzyme-b in children with nephrotic syndrome. Thirty children with biopsy-proven nephrotic syndrome as well as twenty four healthy controls were evaluated in this study. Clinical and laboratory data of included cases and controls were collected. Serum Fas-ligand and granzyme-b levels were measured using specific enzyme-linked immunosorbent assay (ELISA). The results of the current study showed that children with nephrotic syndrome had significantly higher serum levels of Fas-ligand (183.29±43.34pg/ml) and granzyme-b (151.68±30.47pg/ml) than healthy control subjects (Fas-1=99.28±7.20pg/ml, and granzyme-b=86.10±5.68pg/ml) (P=0.000). A significant increase in Fas-ligand levels in Focal segme...
Background: The methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism has been shown to be associated with cardiovascular disease and in patients with end-stage renal disease (ESRD). However, the relationship between MTHFR... more
Background: The methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism has been shown to be associated with cardiovascular disease and in patients with end-stage renal disease (ESRD). However, the relationship between MTHFR polymorphisms and cardiovascular disease (CVD) in patients on hemodialysis has not been examined. The aim of this study was to assess the association of polymorphisms of MTHFR gene with homocysteine (Hcy) and intimal medial thickness (IMT) in children on hemodialysis. Methods: We performed a casecontrol study comprising 55 pediatric patients with ESRD and 55 healthy children as controls. Plasma Hcy was measured in all the subjects and these subjects were genotyped for 2 MTHFR polymorphisms (C677T and A1298C). Results: We observed significantly higher Hcy levels in patients compared to controls. The frequency of MTHFR 1298CC genotype was significantly higher in ESRD children than in controls (21.82 % vs 5.45 %) and the frequency of the MTHFR 677TT gen...
Background: 16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR) is used for detection of bacterial pathogens in the serum of patients with acute pancreatitis (AP). Acute pancreatitis is a frequent cause of hospital admission.... more
Background: 16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR) is used for detection of bacterial pathogens in the serum of patients with acute pancreatitis (AP). Acute pancreatitis is a frequent cause of hospital admission. Infection and septic complications are the major factors contributing to the poor outcome in acute severe pancreatitis. PCR makes it possible to identify the presence of bacterial DNA in culture-negative samples from patients with suspected infection. This molecular approach allows non-culture rapid confirmation of pancreatitis and septicemia, which leads to improved disease surveillance and provides guidance on appropriate antibiotic usage and patient management. The aim of the present study is to detect bacterial DNA accurately by PCR targeting 16s rRNA as a common bacterial primer in serum of patients with acute pancreatitis in order to augment the standardized use of prophylactic antibiotics in such cases. Methods: twenty patients with acute pancr...
BACKGROUND: Anemia is one of the most common complications in end-stage renal disease (ESRD) patients. Hepcidin is a hormone that regulates iron homeostasis in patients with ESRD. Carnitine deficiency is commonly seen in hemodialysis (HD)... more
BACKGROUND: Anemia is one of the most common complications in end-stage renal disease (ESRD) patients. Hepcidin is a hormone that regulates iron homeostasis in patients with ESRD. Carnitine deficiency is commonly seen in hemodialysis (HD) patients. AIM: This study aimed to investigate the relationship between hepcidin and inflammatory and other anemia markers in children with ESRD and to evaluate the association of carnitine deficiency with anemia in these patients. SUBJECTS AND METHODS: Thirty pediatric patients with ESRD undergoing HD, and thirty healthy, age- and sex-matched children served as controls were included in the study. Serum levels hepcidin, iron status, high-sensitivity C-reactive protein, and total carnitine were measured. RESULTS: Statistically significant increases in serum levels of hepcidin (100.7 ± 0.99 ng\ml vs. 77.43 ± 0.8 ng\ml, p = 0.000), was found in HD children as compared to healthy controls. Statistically significant increase in serum levels of hs-CRP (...
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BACKGROUND: Hepcidin, a small peptide hormone, is established as the main regulator of iron homeostasis. AIM: To estimate serum hepcidin, ferritin, and hepcidin: ferritin ratio in β-thalassemia patients and to determine the effect of... more
BACKGROUND: Hepcidin, a small peptide hormone, is established as the main regulator of iron homeostasis. AIM: To estimate serum hepcidin, ferritin, and hepcidin: ferritin ratio in β-thalassemia patients and to determine the effect of splenectomy and hydroxyurea on serum hepcidin. METHODS: A study was conducted on 30 thalassemia major (βTM), 29 thalassemia intermedia (βTI) and 29 healthy children's controls. Data were collected by patient interviewing where detailed history-taking and thorough clinical examinations were carried out. Serum ferritin and hepcidin were measured by ELISA assay (Bioneovan Co. Ltd Beijing, China). RESULTS: Βeta-thalassemia patients had higher serum ferritin, serum hepcidin and lower Hb and hepcidin: ferritin ratio compared to the controls (p < 0.001, 0.010, 0.001, 0.001) respectively. Β-TM patients had higher mean serum hepcidin and serum ferritin compared to β-TI, with statistically significant difference (P = 0.042, P < 0.001, respectively). Twe...
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BackgroundKlotho G-395-A gene polymorphism may impact children with end-stage renal disease (ESRD). We investigated the relevance of Klotho G-395-A on ESRD development and progression, and its relationship with evolution of cardiovascular... more
BackgroundKlotho G-395-A gene polymorphism may impact children with end-stage renal disease (ESRD). We investigated the relevance of Klotho G-395-A on ESRD development and progression, and its relationship with evolution of cardiovascular complications in pediatric dialysis patients.MethodsFifty-five children with chronic kidney disease (CKD) and seventy healthy children were genotyped for Klotho G-395A.ResultsIncidence of GA/AA genotypes and A allele were higher in ESRD patients compared with controls (54.5 vs. 7.1%, P < 0.001; 30.9 vs. 13.6%, P = 0.001, respectively). Also, children with GA/AA genotypes were 15.6 times more likely to develop ESRD than with GG genotype (95% CI 5.4–44.7, P < 0.001). A allele carriers have 2.8 times higher risk of developing ESRD than those with G allele (95% CI 1.5–5.35, P = 0.001). Also, the A allele could be considered a predictor of cardiovascular disease (CVD), as carriers have 161 times higher risk of cardiovascular complications than non-carriers (95% CI 21–1233, P < 0.001). All ESRD patients with CVD presented with left ventricular hypertrophy (LVH) and the frequency of A allele was significantly higher among ESRD children with LVH, whereas G allele frequency was significantly higher among ESRD children without LVH.ConclusionsThe A allele of the G-395A Klotho gene polymorphism shows a significantly higher frequency among children with CKD and those with CVD and LVH. This mutant allele could be used as a risk marker for the development of ESRD as well as a predictor of CVD in these children.
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Background Laser acupuncture is one of the complementary modalities used for treating osteoarthritis. The study was performed to evaluate the effectiveness of laser acupuncture in the treatment of grade 2 knee osteoarthritis. Patients and... more
Background Laser acupuncture is one of the complementary modalities used for treating osteoarthritis. The study was performed to evaluate the effectiveness of laser acupuncture in the treatment of grade 2 knee osteoarthritis. Patients and methods Forty patients having bilateral knee osteoarthritis were divided into two groups (20 patients in each group). The patients of the first group were subjected to 12 laser sessions at the following acupoints (St 35, St36, Sp9, Sp10 and Gb 34). During each session, laser of 90 mw was directed to each acupoint for 1 min giving energy of 5.4 joules. Energy of 21.6 joules was directed to ashi points. The laser had a wavelength of 808 nm, beam diameter 2 mm and was applied with a continuous wave. The cases of the second group were used as controls. Each patient is exposed to sham laser (laser probe is directed to the same acupoints while the device is off). Results The 20 patients receiving laser showed significant improvement in pain on (VAS), inc...
Research Interests: Complementary and Alternative Medicine, Plant Biology, Treatment Outcome, Medicine, Prospective studies, and 13 moreHumans, Lasers, Female, Male, Aged, Middle Aged, Adult, Knee Osteoarthritis, Substance P, Integrative and Complementary Medicine, Beta-endorphin, Acupuncture points, and Pharmacology and pharmaceutical sciences
Fc gamma receptor (FcγR) IIa is considered the most widely distributed of the three classes of Fc receptors, and it expresses an allelic polymorphism. This type of polymorphism may modify the immune response and may be an important factor... more
Fc gamma receptor (FcγR) IIa is considered the most widely distributed of the three classes of Fc receptors, and it expresses an allelic polymorphism. This type of polymorphism may modify the immune response and may be an important factor for some diseases. The aim of the study reported herein was to evaluate the association between the FcγRIIa polymorphism and susceptibility to both end-stage renal disease (ESRD) and acute kidney graft rejection (AR) in children who have undergone renal transplantation. The study evaluated 70 children who had undergone transplantation and 60 healthy subjects. AR was observed in 25 children. FcγRIIa genotypes and alleles were significantly different between transplantation patients and the control group. The assessment for FcγR of the groups in which AR was present showed that there was only a risk of having an acute rejection in homozygous genotype RR. FcγRIIa RR genotype and allele frequency was increased in paediatric renal transplant recipients....
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Breast cancer remains one of the top threats to women's health. The current lack of tumor markers with desirable sensitivity and specificity is a major obstacle toward the future management of breast cancer. Many studies are directed... more
Breast cancer remains one of the top threats to women's health. The current lack of tumor markers with desirable sensitivity and specificity is a major obstacle toward the future management of breast cancer. Many studies are directed to reveal the diagnostic and prognostic potentials of circulating miRNAs in breast cancer. In this study, we attempt to evaluate the feasibility and clinical utility of circulating miRNA-21 and let-7 as prognostic biomarkers for breast cancer. Real-time quantitative polymerase chain reaction technique was used. Levels of miRNA-21 and let-7 expression were determined in sera from 125 participants representing 3 different groups. With fold-change analysis, the expression of miRNA-21 and let-7 in the decided groups were assessed. Patients with breast cancer showed significantly higher expression of miRNA-21 compared with controls and other participants with benign breast lesions (P < .001). The mean expression levels of serum miRNA-21 was 3.27 ± 2.1...
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Data concerning the concentration of matrix metalloproteinase-9 (MMP-9) and its functional polymorphisms in chronic kidney diseases (CKD) are conflicting. The present study aimed to evaluate the levels of MMP-9in children with end stage... more
Data concerning the concentration of matrix metalloproteinase-9 (MMP-9) and its functional polymorphisms in chronic kidney diseases (CKD) are conflicting. The present study aimed to evaluate the levels of MMP-9in children with end stage renal diseases (ESRD) on hemodialysis (HD) and to explore its association with MMP-9 polymorphism and vitamin D levels as an important risk factors for cardiovascular diseases (CVD). We studied 55 children with ESRD on hemodialysis and 18 healthy children served as controls. MMP-9 and vitamin D levels were measured by ELISA in serum of all patients and controls. Genotypes for MMP-9 polymorphism(C(-1562)T) were determined by RFLP for only 28 of the patients and all the controls. There were insignificantly reduced MMP-9levels of patients vs. controls, however, there was significant increase in MMP-9 levels associated with CC genotypes for(C(-1562)T) polymorphism compared with CT genotype (p=0.01). We found that at MMP-9 base position-1562, the frequenc...
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Immune thrombocytopenic purpura (ITP) etiology is not clarified. Phospholipid antigen antibodies (aPls) occur in ITP patient sera. We studied predictive values of elevated anti-beta2-glycoprotein I (anti-beta2-GP1) or anticardiolipin... more
Immune thrombocytopenic purpura (ITP) etiology is not clarified. Phospholipid antigen antibodies (aPls) occur in ITP patient sera. We studied predictive values of elevated anti-beta2-glycoprotein I (anti-beta2-GP1) or anticardiolipin antibody (aCl) concentrations for secondary ITP detection, comparing levels with steroid therapy responsiveness in three groups of children and adolescents. Participants&amp;amp;amp;amp;#39; antinuclear antibodies, aCls (IgM, IgG) and anti-beta2-GPI (IgG) were assessed. Significantly higher aCl (IgM), aCl (IgG) and anti-beta2-GPI (IgG) mean concentrations occurred in chronic ITP cases compared with acute or control cases. Of chronic ITP cases, 77.8% showed elevated IgG aCl serum concentrations, and all presented increased IgG anti-beta2-GPI serum levels. Significant positive correlation between increased levels of IgG anti-beta2-GPI and increased IgG aCl serum concentrations was determined; these increased IgG concentrations significantly correlated with steroid therapy resistance. A total of 76.1% of ITP cases had positive aPls (all chronic ITP cases, five acute ITP cases). Elevated aCl or anti-beta2-GPI serum IgG isotype concentrations occurred in all nine splenectomized ITP children with positive aPls (three showed increased IgM aCl levels). Follow-up of the initially studied ITP children (2000-2004) revealed 16.7% developed clinical and laboratory criteria of systemic lupus erythrematosus (one acute ITP in remission, six chronic ITP); elevated IgG aCl serum concentrations were found at study start in these seven cases, and six had increased anti-beta2-GPI. IgG classes of both aCls and anti-beta2-GPI may be determinant cofactors for the developing risk of antiphospholipid syndrome or autoimmune diseases in ITP. Great attention should be paid to both assays as predictors for steroid therapy response.
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ABSTRACT
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Background: Increased oxidative stress is a hallmark of end-stage renal disease (ESRD). Glutathione S transferases (GST) are involved in the detoxification of xenobiotics and protection from oxidative damage. We hypothesized that genetic... more
Background: Increased oxidative stress is a hallmark of end-stage renal disease (ESRD). Glutathione S transferases (GST) are involved in the detoxification of xenobiotics and protection from oxidative damage. We hypothesized that genetic polymorphisms in antioxidant enzymes GSTM1 and GSTT1 are more frequent in ESRD and modulate the degree of oxidative stress in these patients. Objectives: The present study was designed to evaluate oxidized LDA (ox-LDL), high-sensitivity C-reactive protein (hs-CRP), and homocysteine (hcy) levels in children with end-stage renal disease (ESRD) treated with maintenance haemodialysis (MHD) or under conservative treatment, to compare these levels with those assayed in control subjects, and to evaluate these levels with different GSTM1 and GSTT1 genotypes. Methods: This case-control study was conducted in 78 children. They were divided into 3 groups: group I (44 on HD), group II (14 on conservative treatment), and group III (20 normal healthy children) se...
Background : End stage renal disease (ESRD) is a common cause of morbidity and mortality among children. Interleukin 4 is a cytokine that might influence the progression of chronic kidney disease (CKD) to end stage renal disease. There... more
Background : End stage renal disease (ESRD) is a common cause of morbidity and mortality among children. Interleukin 4 is a cytokine that might influence the progression of chronic kidney disease (CKD) to end stage renal disease. There are variable number of tandem repeats (VNTRs) in IL4 gene that could play major roles in genetic predisposition to some diseases. Aim of the study: The purpose of this study is to detect the association of allelic variant in intron 3 VNTR-IL4 gene with the end stage renal disease and if these variants could be considered as risk markers for this disease. Subjects and methods : The study was conducted on fifty-five children with CKD and fifty healthy children served as controls. All participants were genotyped for intron 3 VNTR by Polymerase Chain Reaction. Results : The frequency of intron 3 VNTR-IL4 P1P2 + P2P2 genotypes was significantly higher in ESRDchildren than those with P1P1 genotype (88.7% vs. 15.4%, OR 43; 95% CI 13–134, P value < 0.001). Also, the frequency of P2 allele was significantly higher in ESRD-children compared with healthy controls (70.9% vs. 8%, OR 28; 95% CI 12–64, P value < 0.001). Furthermore, a significantly higher frequencies of P1P1 genotype and P1 allele among the control group were demonstrated (84.6% vs. 11.3%, P < 0.001 and 92% vs. 29.1%, P < 0.001, respectively). Conclusion : we concluded that the P2 allele is an allelic variant predisposing to ESRD in children with CKD and it could be considered a risk factor for the development of ESRD. Keywords : VNTR-IL4 ESRD PCR Children Gene polymorphism
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Nitric oxide is a free radical that is elevated in the plasma of patients with heart failure due to contractile dysfunction. This study examines the relation between plasma NO level and left ventricular (LV) diastolic function and its... more
Nitric oxide is a free radical that is elevated in the plasma of patients with heart failure due to contractile dysfunction. This study examines the relation between plasma NO level and left ventricular (LV) diastolic function and its aetiology in heart failure patients in the pediatric age group. We performed echocardiographic Doppler studies in 47 patients (mean age 6.16 ±