There is an urgent need to develop alternative to kidney biopsy in children to diagnose nephrotic... more There is an urgent need to develop alternative to kidney biopsy in children to diagnose nephrotic syndrome. Increased apoptosis plays a central role in the development of nephrotic syndrome. The aim of this study was to evaluate the blood levels of two markers of apoptosis, Fas-ligand (Fas-l) and granzyme-b in children with nephrotic syndrome. Thirty children with biopsy-proven nephrotic syndrome as well as twenty four healthy controls were evaluated in this study. Clinical and laboratory data of included cases and controls were collected. Serum Fas-ligand and granzyme-b levels were measured using specific enzyme-linked immunosorbent assay (ELISA). The results of the current study showed that children with nephrotic syndrome had significantly higher serum levels of Fas-ligand (183.29±43.34pg/ml) and granzyme-b (151.68±30.47pg/ml) than healthy control subjects (Fas-1=99.28±7.20pg/ml, and granzyme-b=86.10±5.68pg/ml) (P=0.000). A significant increase in Fas-ligand levels in Focal segme...
Background: Several vitamin D receptor polymorphisms have been associated with SLE development an... more Background: Several vitamin D receptor polymorphisms have been associated with SLE development and clinical manifestations. So, the aim of the study was to evaluate the possible association between the common VDR gene polymorphisms and SLE susceptibility and severity in children. Patients and methods: Thirty six lupus children, 29 females (80.6%) and 7 males (19.6 %) were recruited from the Collagen Clinic, Children Hospital, Cairo University between February and November, 2016. Forty three healthy age and sex matched children were included as a control group. Five ml venous blood sample was withdrawn under aseptic conditions from the studied groups. Two ml were put in a sterile tube containing EDTA for genotyping of BsmI (rs1544410), ApaI (rs7975232), TaqI (rs731236) and FokI (rs2228570) polymorphisms by real time PCR. The rest was centrifuged and serum was used for assessment of: serum calcium, vitamin D level, total and intact Parathormonee (IPTH) level, and fibroblast growth fac...
The epidemic of overweight and obesity in youth is increasing the prevalence of prehypertension a... more The epidemic of overweight and obesity in youth is increasing the prevalence of prehypertension and hypertension among children and adolescents. The younger the child is at presentation and the more severe the blood pressure abnormality, the more likely a secondary cause of hypertension to be present. Measurement of blood pressure (BP) in children requires adaptation to the age and size of the children. Interpretation must be related to normative values specific to age, gender and height. Evaluation is primarily aimed at identifying secondary causes of hypertension, associated comorbidities, additional risk factors, and evidence of target organ damage. Ambulatory blood pressure monitoring is emerging as a useful tool for evaluation of some patients, particularly for those with suspected white coat hypertension. Uncontrolled and prolonged elevation of blood pressure can lead to a variety of changes in the myocardial structure, coronary vasculature, and conduction system of the heart....
Background: Acute kidney injury (AKI) is a serious postoperative complication after cardiac surge... more Background: Acute kidney injury (AKI) is a serious postoperative complication after cardiac surgery in children and is a major contributor to patient outcome. This study aims to identify the incidence of AKI in children undergoing cardiac surgery and the role of Interleukin 18 (IL-18) and Kidney Injury Molecule 1 (KIM-1) in diagnosis of AKI in comparison to creatinine. Methods: Forty-four children who underwent open heart surgery using cardiopulmonary bypass) for congenital heart disease were assessed for AKI diagnosis according the KDIGO criteria, urinary IL-18 and KIM-1 were determined by Enzyme Linked Immunosorbent Assay in addition to the assessment of length of stay in PICU and outcome and the effect of AKI on these parameters. Results: twenty three percent of the patient developed AKI, there were no statistical correlation between AKI and the factors (Age, gender, CBP and Risk adjustment for congenital heart surgery (RACHS-1) complexity score). Eight of our patients needed per...
s 19th International Congress of Nutrition October 4–9, 2009, Bangkok, Thailand Editors: Tontisir... more s 19th International Congress of Nutrition October 4–9, 2009, Bangkok, Thailand Editors: Tontisirin, K.; Wasantwisut, E. (Bangkok)
Introduction: Regulatory T cells (Treg) are emerging as a potential therapy to facilitate long-te... more Introduction: Regulatory T cells (Treg) are emerging as a potential therapy to facilitate long-term allograft survival what makes identification of reliable surface markers that are selectively expressed on Treg is crucial. The aim of this study is to evaluate the regulatory and suppressive functions of CD127 in peripheral T lymphocytes of pediatric kidney transplant recipients through studying the association their frequency and the development of rejection. Material and methods: Flow cytometric analysis of peripheral blood samples for the CD127 surface marker of 50 pediatric transplant recipients and 12 healthy controls was done. Clinical, laboratory, immunosuppressive therapy data and graft function of transplant recipients were collected and correlated with their CD127 peripheral blood expression. Results: CD127 expression in transplanted children was significantly elevated than that of controls (2.76 ±3.26% vs. 0.95 ±0.94%, p = 0.042). CD 127 expression did not correlate with d...
Background: The equilibrium between regulatory cells and cytotoxic cells may define graft consequ... more Background: The equilibrium between regulatory cells and cytotoxic cells may define graft consequence. We investigated the relationship between the expression of main regulatory and cytotoxic markers (i.e., FOXP3 and granzyme B (GZM-B), respectively) and acute rejection (AR) in the peripheral blood of pediatric renal transplant recipients. Methods: In this retrospective study, FOXP3 mRNA expression and serum GZM-B levels in peripheral blood samples from 47 first-time pediatric kidney-transplant recipients were measured, with 17 children classified as possessing AR; whereas the remaining 30 children had functionally stabilized allografts. Results: Levels of the FOXP3 mRNA vs. the expression levels GADPH mRNA (FOXP3 mRNA/GADPH mRNA) were significantly elevated in children with AR than those with stabilized renal allograft (0.48 ± 0.26 vs.0.23 ± 0.18, respectively, P=0.002) Also, serum GZM-B levels in the AR group were elevated than those in the functionally stabilized children (120.07...
There is an urgent need to develop alternative to kidney biopsy in children to diagnose nephrotic... more There is an urgent need to develop alternative to kidney biopsy in children to diagnose nephrotic syndrome. Increased apoptosis plays a central role in the development of nephrotic syndrome. The aim of this study was to evaluate the blood levels of two markers of apoptosis, Fas-ligand (Fas-l) and granzyme-b in children with nephrotic syndrome. Thirty children with biopsy-proven nephrotic syndrome as well as twenty four healthy controls were evaluated in this study. Clinical and laboratory data of included cases and controls were collected. Serum Fas-ligand and granzyme-b levels were measured using specific enzyme-linked immunosorbent assay (ELISA). The results of the current study showed that children with nephrotic syndrome had significantly higher serum levels of Fas-ligand (183.29±43.34pg/ml) and granzyme-b (151.68±30.47pg/ml) than healthy control subjects (Fas-1=99.28±7.20pg/ml, and granzyme-b=86.10±5.68pg/ml) (P=0.000). A significant increase in Fas-ligand levels in Focal segme...
Background: Several vitamin D receptor polymorphisms have been associated with SLE development an... more Background: Several vitamin D receptor polymorphisms have been associated with SLE development and clinical manifestations. So, the aim of the study was to evaluate the possible association between the common VDR gene polymorphisms and SLE susceptibility and severity in children. Patients and methods: Thirty six lupus children, 29 females (80.6%) and 7 males (19.6 %) were recruited from the Collagen Clinic, Children Hospital, Cairo University between February and November, 2016. Forty three healthy age and sex matched children were included as a control group. Five ml venous blood sample was withdrawn under aseptic conditions from the studied groups. Two ml were put in a sterile tube containing EDTA for genotyping of BsmI (rs1544410), ApaI (rs7975232), TaqI (rs731236) and FokI (rs2228570) polymorphisms by real time PCR. The rest was centrifuged and serum was used for assessment of: serum calcium, vitamin D level, total and intact Parathormonee (IPTH) level, and fibroblast growth fac...
The epidemic of overweight and obesity in youth is increasing the prevalence of prehypertension a... more The epidemic of overweight and obesity in youth is increasing the prevalence of prehypertension and hypertension among children and adolescents. The younger the child is at presentation and the more severe the blood pressure abnormality, the more likely a secondary cause of hypertension to be present. Measurement of blood pressure (BP) in children requires adaptation to the age and size of the children. Interpretation must be related to normative values specific to age, gender and height. Evaluation is primarily aimed at identifying secondary causes of hypertension, associated comorbidities, additional risk factors, and evidence of target organ damage. Ambulatory blood pressure monitoring is emerging as a useful tool for evaluation of some patients, particularly for those with suspected white coat hypertension. Uncontrolled and prolonged elevation of blood pressure can lead to a variety of changes in the myocardial structure, coronary vasculature, and conduction system of the heart....
Background: Acute kidney injury (AKI) is a serious postoperative complication after cardiac surge... more Background: Acute kidney injury (AKI) is a serious postoperative complication after cardiac surgery in children and is a major contributor to patient outcome. This study aims to identify the incidence of AKI in children undergoing cardiac surgery and the role of Interleukin 18 (IL-18) and Kidney Injury Molecule 1 (KIM-1) in diagnosis of AKI in comparison to creatinine. Methods: Forty-four children who underwent open heart surgery using cardiopulmonary bypass) for congenital heart disease were assessed for AKI diagnosis according the KDIGO criteria, urinary IL-18 and KIM-1 were determined by Enzyme Linked Immunosorbent Assay in addition to the assessment of length of stay in PICU and outcome and the effect of AKI on these parameters. Results: twenty three percent of the patient developed AKI, there were no statistical correlation between AKI and the factors (Age, gender, CBP and Risk adjustment for congenital heart surgery (RACHS-1) complexity score). Eight of our patients needed per...
s 19th International Congress of Nutrition October 4–9, 2009, Bangkok, Thailand Editors: Tontisir... more s 19th International Congress of Nutrition October 4–9, 2009, Bangkok, Thailand Editors: Tontisirin, K.; Wasantwisut, E. (Bangkok)
Introduction: Regulatory T cells (Treg) are emerging as a potential therapy to facilitate long-te... more Introduction: Regulatory T cells (Treg) are emerging as a potential therapy to facilitate long-term allograft survival what makes identification of reliable surface markers that are selectively expressed on Treg is crucial. The aim of this study is to evaluate the regulatory and suppressive functions of CD127 in peripheral T lymphocytes of pediatric kidney transplant recipients through studying the association their frequency and the development of rejection. Material and methods: Flow cytometric analysis of peripheral blood samples for the CD127 surface marker of 50 pediatric transplant recipients and 12 healthy controls was done. Clinical, laboratory, immunosuppressive therapy data and graft function of transplant recipients were collected and correlated with their CD127 peripheral blood expression. Results: CD127 expression in transplanted children was significantly elevated than that of controls (2.76 ±3.26% vs. 0.95 ±0.94%, p = 0.042). CD 127 expression did not correlate with d...
Background: The equilibrium between regulatory cells and cytotoxic cells may define graft consequ... more Background: The equilibrium between regulatory cells and cytotoxic cells may define graft consequence. We investigated the relationship between the expression of main regulatory and cytotoxic markers (i.e., FOXP3 and granzyme B (GZM-B), respectively) and acute rejection (AR) in the peripheral blood of pediatric renal transplant recipients. Methods: In this retrospective study, FOXP3 mRNA expression and serum GZM-B levels in peripheral blood samples from 47 first-time pediatric kidney-transplant recipients were measured, with 17 children classified as possessing AR; whereas the remaining 30 children had functionally stabilized allografts. Results: Levels of the FOXP3 mRNA vs. the expression levels GADPH mRNA (FOXP3 mRNA/GADPH mRNA) were significantly elevated in children with AR than those with stabilized renal allograft (0.48 ± 0.26 vs.0.23 ± 0.18, respectively, P=0.002) Also, serum GZM-B levels in the AR group were elevated than those in the functionally stabilized children (120.07...
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