Aziza Shalby
National Research Centre, Hormones, Post-Doc
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Objective: This research was conducted to explore mechanisms behind the potency of quercetin in inhibiting colon cancer induced in an experimental model. Materials and Methods: Forty adult male rats of Wistar strain were distributed into... more
Objective: This research was conducted to explore mechanisms behind the potency of quercetin in inhibiting colon cancer induced in an experimental model. Materials and Methods: Forty adult male rats of Wistar strain were distributed into 4 groups; a negative control group, a colon cancer bearing group, a quercetin-treated group and a 5-fluorouracil (5-FU)-treated group. Serum TAG72 and GAL3 levels were quantified by ELISA. Colonic Wnt5a and Axin-1 gene expression was estimated by PCR. In addition, colonic tissues were subjected to immunohistochemical examination of Bax expression and histological investigation of histopathological alterations. Results: Quercetin elicited significant reduction in serum TAG72 and GAL3 levels, in addition to significant suppression of colonic Wnt5a gene expression and amplification of colonic Axin-1 gene expression. Also, it caused moderate positive reaction for Bax in mucosal epithelium. Conclusion: The present research provides experimental evidence about the activity of quercetin in the colon cancer of rats. Inhibitory effects on cancer development might be ascribable to regulatory action on Wnt signaling and induction of apoptosis.
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This research work was initiated to explore the efficacy of ellagic acid in mitigation of hepatocarcinogenesis in rats. Rats were distributed into 4 groups; negative control, hepatocellular carcinoma (HCC), doxorubicin and ellagic acid.... more
This research work was initiated to explore the efficacy of ellagic acid in mitigation of hepatocarcinogenesis in rats. Rats were distributed into 4 groups; negative control, hepatocellular carcinoma (HCC), doxorubicin and ellagic acid. Serum alpha-fetoprotein (AFP), glypican-3 (GPC-3), signal transducer and activator of transcription 3 (STAT3) and suppressors of cytokine signaling 3 (SOCS3) levels were assayed by ELISA. Immunohistochemical examination of hepatic VEGF expression was also conducted, along with histological procedures for examination of liver tissue sections. Significant elevation in serum AFP, GPC-3 and STAT3 levels with a significant drop in SOCS3 were observed in the HCC group. In contrast, the treated groups showed significant decline in serum AFP, GPC-3 and STAT3 levels and significant increase in SOCS3. Additionally, the HCC group declared mild positive immunoreaction for VEGF in hepatocytes while treatment with doxorubicin or ellagic acid was associated with a negative immunoreaction for VEGF. These results were supported by histological examination of liver tissue. The obtained findings suggested that ellagic acid may have beneficial chemopreventive role against hepatocarcinogenesis through its apoptotic, antiangiogenic and antiproliferative activities.
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Cancer, a worldwide epidemic disease with diverse origins, involves abnormal cell growth with the potential to invade other parts of the body. Globally, it is the main cause of mortality and morbidity. To overcome the drawbacks of the... more
Cancer, a worldwide epidemic disease with diverse origins, involves abnormal cell growth with the potential to invade other parts of the body. Globally, it is the main cause of mortality and morbidity. To overcome the drawbacks of the commercially available chemotherapies, natural products-loaded nano-composites are recommended to improve cancer targetability and decrease the harmful impact on normal cells. This study aimed at exploring the anti-cancer impacts of Moringa oleifera seed oil in its free- (MO) and nano-formulations (MOn) through studying whether it mechanistically promotes mitochondrial apoptosis-mediating cell death. Mitochondrial-based cytotoxicity and flow cytometric-based apoptosis analyses were performed on cancer HepG2, MCF7, HCT 116, and Caco-2 cell lines against normal kidney BHK-21 cell line. The present study resulted that MOn triggered colorectal cancer Caco-2 and HCT 116 cytotoxicity via mitochondrial dysfunction more powerful than its free counterpart (MO). On the other side, MOn and MO remarkably induces HCT 116 mitochondrial apoptosis, while sparing normal BHK-21 cells with minimal cytotoxic effect. The present results concluded that nano-micelle of Moringa oleifera seed oil (MOn) can provide a novel therapeutic approach for colorectal and breast cancers via mitochondrial-mediated apoptosis, while sparing normal and even liver cancer cells a bit healthy or with minimal harmful effect. Intriguingly, MOn induced breast cancer not hepatocellular carcinoma cell death.
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Research Interests: Endocrinology, Biology, Crop Physiology and Biochemistry, Medicine, Gene expression, and 15 moreMelatonin, Dexamethasone, Brain, Internal Medicine, Female, Animals, Antioxidant, Male, Glutathione, Acetylcholinesterase, Heme Oxygenase, Medical Physiology, Glucocorticoids, Malondialdehyde, and DNA fragmentation
Cisplatin (CP), a broadly used anticancer drug, is widely known to induce acute renal failure as a result of renal tubular injury. In this study, the effect of N-acetyl cysteine (NAC) or taurine (TAU) for protection against CP-induced... more
Cisplatin (CP), a broadly used anticancer drug, is widely known to induce acute renal failure as a result of renal tubular injury. In this study, the effect of N-acetyl cysteine (NAC) or taurine (TAU) for protection against CP-induced nephrotoxicity was investigated. A single dose of CP 1 mg/kg b.wt. for 4 days was given IP to the rats, 10 days rest, and then the dose was repeated for other 4 days. After CP administration, NAC or TAU was given IP in a dose of 50 mg/kg b.wt. 3 times weekly for 8 weeks. CP-induced nephrotoxicity is reflected in high values of blood urea and creatinine levels. In addition, the significant increase in the β(2)-MG and N-acetyl-β-glucosaminidase enzymes exhibited a strong correlation with histology scores of overall proximal tubule damage as compared with the normal control values. Treatment with TAU or NAC after CP administration significantly ameliorated CP-induced nephritic oxidation stress markers as compared with CP-treated group. On the other hand, treatment with TAU or NAC after CP administration significantly ameliorated CP-induced nephritic inflammation with possible attenuation of renal injury. These data suggest that oxidative stress and inflammation are involved in the pathogenesis of CP-induced acute renal failure. The inhibition in oxidative stress and the elevation of the total antioxidant capacity as well as the inhibition of the inflammatory biomarkers by NAC or TAU after CP administration may play a central role in modulation of nephrotoxicity induced by CP. NAC and TAU have antioxidant and anti-inflammatory against CP-induced nephrotoxicity.
Research Interests: Pharmacology, Chemistry, Acute kidney injury, Oxidative Stress, Medicine, and 15 moreAntioxidants, Kidney, Female, Animals, Lipid peroxidation, Cisplatin, Rats, Biological markers, Taurine, Nephrotoxicity, Antineoplastic Agents, Creatinine, Pharmacology and pharmaceutical sciences, Kidney function tests, and acetylcysteine
Hepatocarcinoma (HCC) and colorectal cancer (CRC) are the most malignant tumors with increasing incidences of morbidity and mortality globally.The aim of the present study was to determine in vitro antitumor activity of quercetin, gallic... more
Hepatocarcinoma (HCC) and colorectal cancer (CRC) are the most malignant tumors with increasing incidences of morbidity and mortality globally.The aim of the present study was to determine in vitro antitumor activity of quercetin, gallic acid, and ellagic acid against HCC and CRC. This was achieved through testing the effects of these compounds on HepG2 and HCT 116 cells, ranging from cell growth inhibition assay and fold dose advantage, passing by mitochondrial activity assessment, to cellular protein content measurement. The efficacies of these compounds were compared with doxorubicin in case of HepG2 cells and 5-fluorouracil in case of HCT 116 cells. The present findings indicated that in both cell types, gallic acid elicited the most pronounced anti-cancer activity and mitochondrial dysfunction compared to quercetin and ellagic acid. Extensive research is warranted to assess the precise molecular mechanisms of these natural products in bridling HCC and CRC.
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Research Interests: Endocrinology, Medicine, Gene expression, Estrogen Receptor, Internal Medicine, and 15 moreCurcumin, Animals, Male, Hepatocellular Carcinoma, Rats, Public health systems and services research, Wistar Rats, Androgen Receptor, Monoterpenes, Diethylnitrosamine, Estrogen receptor alpha, Carvacrol, Liver neoplasms, Estrogen Receptor beta, and Biological products
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Background: Postmenopausal osteoporosis has become a global health issues recently. Several vitamins play a role in building and maintenance of bones. Objective: To evaluate the possible roles of vitamin B-complex supplementation alone or... more
Background: Postmenopausal osteoporosis has become a global health issues recently. Several vitamins play a role in building and maintenance of bones. Objective: To evaluate the possible roles of vitamin B-complex supplementation alone or combined with estrogen in the improvement of osteoporosis in ovariectomized (OVX) rats. Materials and Methods: Fifty adult female albino rats were equally divided into 5 groups then 4 groups were exposed to ovariectomy: Control group, OVX group, OVX/ Ethinyl estradiol (EE) group, OVX/B-complex group and OVX/EE/B-complex only. All treatments were started 4 weeks after surgery and continued for 8 weeks. Blood samples were collected to measure serum levels of homocysteine (HCY), bone–specific alkaline phosphatase (BAP), collagen type 1 cross-linked C-telopeptide (CTX) and calcium (Ca++). Results: Ovariectomy increased serum levels of Ca++, bone turnover markers (BAP and CTX) and homocysteine (HCY). Serum Ca++ levels were increased, while serum levels ...
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Colorectal cancer is associated with significant morbidity and mortality worldwide. Egypt, as a developing country, has a high-rise incidence of cancer. The current study objective was to investigate the antitumor influences of... more
Colorectal cancer is associated with significant morbidity and mortality worldwide. Egypt, as a developing country, has a high-rise incidence of cancer. The current study objective was to investigate the antitumor influences of ellagitannin-loaded CS-PEG-decorated PLGA nano-prototypes against human colorectal cancer cell lines (HCT 116 as well as Caco-2) in vitro. Doxorubicin (DOX), punicalin (PN), and punicalagin (PNG)-encapsulated chitosan-polyethylene glycol-decorated PLGA (PLGA-CS-PEG) nanoparticles (NPs) were described. The cytotoxicity of each preparation was evaluated using MTT assays in HCT 116 as well as Caco-2 cells during G0, G1, S, and G2 cell cycle phases. Cell cycle-related gene expression and protein levels were measured after treatment. Reactive oxygen species (ROS) levels were also measured. Both PN and PNG PLGA-CS-PEG NPs induce colon cancer cell death with cell cycle arrest in the G1 phase in vitro. Caco-2 cells were more sensitive to the nano-therapy than HCT 116...
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The assessment of alteration of postmortem RNA expression has forensic significance in estimating postmortem interval. To evaluate wound healing progression and the effect of different postmortem intervals, histopathological changes,... more
The assessment of alteration of postmortem RNA expression has forensic significance in estimating postmortem interval. To evaluate wound healing progression and the effect of different postmortem intervals, histopathological changes, immunohistochemical matrix metalloproteinase-9 (MMP-9) expression, and long noncoding fatty acid oxidation (lncFAO), RNA expression was assessed in the incised cutaneous wound model. A full-thickness cutaneous wound was inflicted on 75 rats. All 15 rats were sacrificed at different post-infliction intervals (0, 2, 4, 8 and 10 days), and the cutaneous wounds (n = 5) were excised at different postmortem intervals (0, 5, and 24 h after euthanasia). The maximal inflammatory healing stage was detected at day 4 post-infliction, while near complete healing, thick mature collagen deposition was detected at day 10 post-infliction. LncFAO expression was significantly over-expressed with increasing wound age. MMP-9 was detectable on injury day with continuous elev...
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This study examined the preventive and therapeutic benefits of various Panax ginseng fractions against NG-nitro-L-arginine methyl ester (L-NAME)-induced hypertension in rats. Rats injected with L-NAME plus vehicle exhibited persistently... more
This study examined the preventive and therapeutic benefits of various Panax ginseng fractions against NG-nitro-L-arginine methyl ester (L-NAME)-induced hypertension in rats. Rats injected with L-NAME plus vehicle exhibited persistently elevated SBP, serum concentrations of the cardiac injury biomarkers creatine kinase (CK), CK-MB, and troponin, total cholesterol, triglyceride (TG), low-density lipoprotein (LDL), and interleukin (IL)-6, but lower high-density lipoprotein (HDL) compared to the vehicle control group. Furthermore, L-NAME disrupted the normal histological structure and function of rat cardiac tissue. All ginseng fractions and losartan restored SBP to a normal level, reversed the changes in CK, CK-MB, troponin, total cholesterol, TG, HDL, and LDH, and preserved normal myocardial histology, with the WGF demonstrating the greatest cardioprotective and antihypertensive effects. Compounds within multiple ginseng fractions, but especially the aqueous fraction, possess potent ...
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Research Interests: Biology and DNA damage
The current study aimed to evaluate the potential role of dehydroepiandrosterone (DHEA) in the protection and intervention of glucocorticoid-induced secondary osteoporosis in female rats. For this purpose this study was conducted on five... more
The current study aimed to evaluate the potential role of dehydroepiandrosterone (DHEA) in the protection and intervention of glucocorticoid-induced secondary osteoporosis in female rats. For this purpose this study was conducted on five groups of female Sprague Dawley rats which were classified into: (1) negative control group received saline as vehicle, (2) osteoporotic group orally administered with prednisolone (5 mg/kg b.wt.) daily for six months, (3) positive control group orally administered with DHEA (250 mg/kg b.wt.) three times weekly for six months, (4) protective group orally administered with prednisolone daily with simultaneous oral administration of DHEA three times weekly for six months and (5) therapeutic group orally administered with prednisolone daily for six months then orally administered with DHEA three times weekly for other six months. The obtained data revealed that prednisolone administration resulted in significant decrease in serum osteocalcin (OC), 1,25-dihydroxyvitamin D(3) (1,25-(OH)(2) D(3)) and osteoprotegerin (OPG) levels accompanied with significant increase in serum parathyroid hormone (PTH) and receptor activator nuclear factor kappa B ligand (RANKL) levels. Histopathological investigation of left femur bone showed that prednisolone administration produced compression of the reduced articular surface and atrophy of the epiphyseal bone. On the other hand, DHEA supplementation to osteoporotic rats increased serum OC, 1,25-(OH)(2) D(3) and OPG levels while decreased serum PTH and RANKL levels. Moreover, DHEA administration resulted in restoration of intact epiphyseal bony structure and articular surface. In conclusion, DHEA via its control on glucocorticoid activity and androgenic action provided potent effect on bone.
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Alzheimer's disease (AD) is now the most common cause of dementia in the elderly. This study aimed to explore new markers for AD as total homocysteine (tHcy), insulin, insulin like growth factor-1 (IGF-1), interleukin-1β (IL-1 β) and... more
Alzheimer's disease (AD) is now the most common cause of dementia in the elderly. This study aimed to explore new markers for AD as total homocysteine (tHcy), insulin, insulin like growth factor-1 (IGF-1), interleukin-1β (IL-1 β) and tumor necrosis factor-α (TNF-α); to determine the modulatory effects of vitamin E (VE), acetyl-L-carnitine (ALC) and α-lipoic acid (LA) on the investigated parameters and to evaluate the possible mechanisms for the role of these nutraceutical in regression of AD that is induced in female rats. Our results revealed that brain acetylcholine esterase (AChE) activity and tHcy levels were significantly increased in AD model. Folic acid, vitamin B 12 levels and Na + /K + ATPase activity were markedly reduced. Plasma insulin and IGF-1 levels were noticeably decreased while plasma TNF-α and IL-1β concentrations were significantly increased, indicating that abnormal inflammatory response is associated with AD. Treatment by VE, ALC or LA restored the above me...
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The present study assessed the potential role of leptin administration in the protection and intervention against glucocorticoid-induced secondary osteoporosis in female rats. For this purpose five groups of female Sprague Dawley rats... more
The present study assessed the potential role of leptin administration in the protection and intervention against glucocorticoid-induced secondary osteoporosis in female rats. For this purpose five groups of female Sprague Dawley rats were classified into: (1) negative control group in which the healthy rats received saline as vehicle, (2) a group orally administered with prednisolone (5 mg kg b.wt.-1) daily for six months (osteoporotic group), (3) a group subcutaneously administered with leptin (400 microg kg b.wt.-1) three times weekly for six months (positive control), (4) a group orally administered with prednisolone daily with simultaneous subcutaneous administration of leptin three times weekly for six months (protective group), and (5) a group orally administered with prednisolone daily for six months then subcutaneously administered with leptin three times weekly for other six months (therapeutic group). The obtained data revealed that prednisolone administration resulted in...
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This study was undertaken to investigate the anti-inflammatory role of fennel, carob, doum and mixture of them in improving the renal dysfunction induced in rats by the cyclosporine. Sixty female albino rats were divided into six groups:... more
This study was undertaken to investigate the anti-inflammatory role of fennel, carob, doum and mixture of them in improving the renal dysfunction induced in rats by the cyclosporine. Sixty female albino rats were divided into six groups: healthy control rats, cyclosporine injected rats (positive control), other groups were injected by cyclosporine for 7 days and then diet was supplemented with either fennel, doum, carob or mixture of them. After 45 days of supplementation, rats were scarified. Serum and urinary samples were obtained for different biochemical analysis. The analysis included the determination of creatinine levels in serum and urinary samples, serum ammonia, transforming growth factor-beta1 (TGF-beta1) and tumor necrosis factor-alpha (TNF-alpha), urinary N-acetyl-beta D-glucosaminidase (NAG), beta2 microglobulin as well as calculating the creatinine clearance. Also, an histopathological examination for the kidney tissue was performed. The present study showed that cycl...
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The present study was planned to investigate the role of sex hormone receptor gene expression in the pathogenesis of hepatocellular carcinoma (HCC). Adult male Wistar rats were divided into seven groups. Group (1) was negative control.... more
The present study was planned to investigate the role of sex hormone receptor gene expression in the pathogenesis of hepatocellular carcinoma (HCC). Adult male Wistar rats were divided into seven groups. Group (1) was negative control. Groups (2), (5), (6), and (7) were orally administered with N-nitrosodiethylamine for the induction of HCC, then group (2) was left untreated, group (5) was orally treated with curcumin, group (6) was orally treated with carvacrol, and group (7) was intraperitoneally injected with doxorubicin, whereas groups (3) and (4) were orally administered only curcumin and carvacrol, respectively. The HCC group showed significant upregulation in the androgen receptor (AR) and the estrogen receptor-alpha (ERα) gene expression levels in the liver tissue. On the contrary, HCC groups treated with either curcumin or carvacrol showed significant downregulation in AR and ERα gene expression levels in the liver tissue. In conclusion, the obtained data highlight that bot...
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Objective: This study aimed to investigate the efficacy of Boswellia serrata methylene chloride extract against colon cancer-induced in rats. Materials and Methods Forty male rats were classified into 5 groups. Group (1) was control.... more
Objective: This study aimed to investigate the efficacy of Boswellia serrata methylene chloride extract against colon cancer-induced in rats. Materials and Methods Forty male rats were classified into 5 groups. Group (1) was control. Groups from (2) to (5) were intrarectally administered with N-methylnitrosourea for induction of colon cancer then group (2) was left untreated (cancer group); group (3) was treated intraperitoneally with 5-fluorouracil, while, groups (4 and 5) were treated orally with 1666.6 mg/kg and 3333.3 mg/kg of Boswellia serrata methylene chloride extract respectively. Serum MMP-7, MMP-9 and EGF, plasma TGF-β and TNF-α levels were assayed using ELISA procedure. Immunohistochemical technique was used for estimation of colon COX-2 and cyclin D1 expression. Colon β-catenin, K-ras and c-myc gene expression was detected by RT-PCR. Also, histological investigation of colon tissue was done. Results: Colon cancer group showed significant elevation in the studied biochemi...
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Alzheimer’s disease (AD) is now the most common cause of dementia in the elderly. This study aimed to explore new markers for AD as total homocysteine (tHcy), insulin, insulin like growth factor-1 (IGF-1), interleukin-1β (IL-1 β) and... more
Alzheimer’s disease (AD) is now the most common cause of dementia in the elderly. This study aimed to explore new markers for AD as total homocysteine (tHcy), insulin, insulin like growth factor-1 (IGF-1), interleukin-1β (IL-1 β) and tumor necrosis factor-α (TNFα); to determine the modulatory effects of vitamin E (VE), acetyl-L-carnitine (ALC) and α-lipoic acid (LA) on the investigated parameters and to evaluate the possible mechanisms for the role of these nutraceutical in regression of AD that is induced in female rats. Our results revealed that brain acetylcholine esterase (AChE) activity and tHcy levels were significantly increased in AD model. Folic acid, vitamin B12 levels and Na/K ATPase activity were markedly reduced. Plasma insulin and IGF-1 levels were noticeably decreased while plasma TNFα and IL-1β concentrations were significantly increased, indicating that abnormal inflammatory response is associated with AD. Treatment by VE, ALC or LA restored the above mentioned para...
2 Abstract: The present study focused on evaluating the effect of different nutraceutical combinations 10 including primarose, omega 3 fatty acids, silymarin, coenzyme Q , antioxidants on tumor growth as well as the metabolic... more
2 Abstract: The present study focused on evaluating the effect of different nutraceutical combinations 10 including primarose, omega 3 fatty acids, silymarin, coenzyme Q , antioxidants on tumor growth as well as the metabolic impact-induced by Ehrlich ascites carcinoma (EAC) inoculation in female mice. After 60 days of oral administration of the selected nutraceutical combinations, the tumor size was recorded. Also, serum and liver samples were obtained for different biochemical analyses. These analyses included the determination of serum arginase, L-fucosidase, lipid peroxidation, nitric oxide levels and total antioxidant capacity. Moreover, hepatic Na - K /ATPase activity, sodium and ++ potassium content of hepatic tissue were also assayed. The present results revealed that EAC inoculation (0.2ml of EAC which contains 2.5×10 cells) causes significant increase in serum arginase, 6 L-fucosidase, , lipid peroxidation, nitric oxide levels and hepatic potassium concentrations, while, t...
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Background/aim Reduction of female sex hormones is associated with health disorders including cardiovascular diseases such as hypertension. Supplementation with vitamin B complex can lower the incidence of these disorders. The present... more
Background/aim Reduction of female sex hormones is associated with health disorders including cardiovascular diseases such as hypertension. Supplementation with vitamin B complex can lower the incidence of these disorders. The present study aimed to study the potential cardioprotective effects of vitamin B complex and ethinyl estradiol (EE) when administered alone or in combination to hormonal-deprived ovariectomized (OVX) rats. Materials and methods Fifty female Wister rats were classified into five equal groups: the first group included normal rats, the second group was OVX (positive control), and the third to fifth groups were OVX rats that were treated by EE (0.03 mg/kg), vitamin B complex (9.036 mg/kg), and a combination of both treatments, respectively. Treatment continued for eight successive weeks. Blood pressure (BP), cystathionine γ-lyase (CSE), reduced glutathione (R-GSH), superoxide dismutase (SOD), catalase (CAT), and interleukin-β6 (IL-β6) were measured. Results The OV...
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Objective: This study aimed to investigate the efficacy of Boswellia serrata methylene chloride extract against colon cancer- induced in rats. Materials and Methods Forty male rats were classified into 5 groups. Group (1) was control.... more
Objective: This study aimed to investigate the efficacy of Boswellia serrata methylene chloride extract against colon cancer- induced in rats. Materials and Methods Forty male rats were classified into 5 groups. Group (1) was control. Groups from (2) to (5) were intrarectally administered with N-methylnitrosourea for induction of colon cancer then group (2) was left untreated (cancer group); group (3) was treated intraperitoneally with 5-fluorouracil, while, groups (4 and 5) were treated orally with 1666.6 mg/kg and 3333.3 mg/kg of Boswellia serrata methylene chloride extract respectively. Serum MMP-7, MMP-9 and EGF, plasma TGF-β and TNF-α levels were assayed using ELISA procedure. Immunohistochemical technique was used for estimation of colon COX-2 and cyclin D1 expression. Colon β-catenin, K-ras and c- myc gene expression was detected by RTPCR. Also, histological investigation of colon tissue was done. Results: Colon cancer group showed significant elevation in the studied biochem...
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The present study aimed to investigate the efficacy of Rosmarinus officinalis total methanolic extract on colon cancer- induced in rats. Rats were classified into 5 groups. Group (1) was control. Groups from (2) to (5) were intrarectally... more
The present study aimed to investigate the efficacy of Rosmarinus officinalis total methanolic extract on colon cancer- induced in rats. Rats were classified into 5 groups. Group (1) was control. Groups from (2) to (5) were intrarectally injected with N-methylnitrosourea (MNU) for induction of colon cancer then group (2) was left untreated (cancer group); group (3) was treated intraperitoneally with 5-fluorouracil while, groups (4 and 5) were treated orally with 1400 and 2800 mg/kg b.wt. of R. officinalis extract respectively. Cancer group declared significant increase in the expression levels of β-catenin and c-Myc genes in colon tissue. Furthermore, colon cancer induced-rats demonstrated significant increase in colon COX-2, cyclin D1 and survivin expression in colon tissue associated with significant elevation in the circulating levels of all studied biochemical markers. Additionally, histopathological investigation of colon tissue sections in cancer group showed dysplasia and ana...
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Nephrotoxicity induced by exposure to environmental pollution, including herbicides, is becoming a global problem. Natural products are the prime alternative scientific research as they express better medicinal activity and minor side... more
Nephrotoxicity induced by exposure to environmental pollution, including herbicides, is becoming a global problem. Natural products are the prime alternative scientific research as they express better medicinal activity and minor side effects compared with a variety of synthetic drugs. This study was performed to evaluate the nephroprotective proficiency of Arabic gum against butralin-induced nephrotoxicity. Adult female rats were supplemented with Arabic gum (4.3 g/kg b.wt) and/or butralin (312 mg/L) in drinking water for 30 days. The results found that markers of serum kidney function, oxidative stress biomarkers, DNA damage, and expression of kidney specific genes (Acsm2, Ace, and Ace2) as well as histopathological examination in treated rats were conducted. Butralin-treated rats showed a rise in serum creatinine (41%), BUN (47.3%), and MDA (140.9%) as well as decrease in activity of the antioxidant markers (CAT (−21%); GPx (−70.7%); and TAC (43.2%)) in comparison with the control group. In addition, butralin treatment increased the DNA damage (221%); altered the expression levels of Acsm2, Ace, and Ace2 (−51.6%, 141.6%, and 143% respectively); and elevated histopathological lesions in the kidney tissues. Pretreatment of Arabic gum prevented butralin-prompted degenerative changes of kidney tissues. The results suggested that the protective effect provided by Arabic gum on renal tissues exposed to the herbicide butralin could be attributed to enhancement of antioxidants and increase the free radical scavenging activity in vivo.
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Drug resistance is a major challenge of breast and colon cancer therapies leading to treatment failure. The main objective of the current study is to investigate whether selenium nanoparticles (nano-Se) can induce the chemo-sensitivity of... more
Drug resistance is a major challenge of breast and colon cancer therapies leading to treatment failure. The main objective of the current study is to investigate whether selenium nanoparticles (nano-Se) can induce the chemo-sensitivity of 5-fluorouracil (FU)-encapsulated poly (D, L-lactide-co-glycolide) nanoparticles (nano-FU) in breast and colon cancer cell lines. Nano-Se and nano-FU were synthesized and characterized, then applied individually or in combination upon MCF7, MDA-MB-231, HCT 116, and Caco-2 cancerous cell lines. Cytotoxicity, cellular glucose uptake, and apoptosis, as well as malondialdehyde (MDA), nitric oxide (NO), and zinc (Zn) levels, were investigated upon the different treatments. We have resulted that nano-FU induced cell death in MCF7 and Caco-2 more effectively than MDA-MB-231 and HCT 116 cell lines. Moreover, nano-FU plus nano-Se potentiate MCF7 and Caco-2 chemo-sensitivity were higher than MDA-MB-231 and HCT 116 cancerous cell lines. It is relevant to note ...
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Cancer, a worldwide epidemic disease with diverse origins, involves abnormal cell growth with the potential to invade other parts of the body. Globally, it is the main cause of mortality and morbidity. To overcome the drawbacks of the... more
Cancer, a worldwide epidemic disease with diverse origins, involves abnormal cell growth with the potential to invade other parts of the body. Globally, it is the main cause of mortality and morbidity. To overcome the drawbacks of the commercially available chemotherapies, natural products-loaded nano-composites are recommended to improve cancer targetability and decrease the harmful impact on normal cells. This study aimed at exploring the anti-cancer impacts of Moringa oleifera seed oil in its free- (MO) and nano-formulations (MOn) through studying whether it mechanistically promotes mitochondrial apoptosis-mediating cell death. Mitochondrial-based cytotoxicity and flow cytometric-based apoptosis analyses were performed on cancer HepG2, MCF7, HCT 116, and Caco-2 cell lines against normal kidney BHK-21 cell line. The present study resulted that MOn triggered colorectal cancer Caco-2 and HCT 116 cytotoxicity via mitochondrial dysfunction more powerful than its free counterpart (MO)....
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Objective: This research was conducted to explore mechanisms behind the potency of quercetin in inhibiting colon cancer induced in an experimental model. Materials and Methods: Forty adult male rats of Wistar strain were distributed into... more
Objective: This research was conducted to explore mechanisms behind the potency of quercetin in inhibiting colon cancer induced in an experimental model. Materials and Methods: Forty adult male rats of Wistar strain were distributed into 4 groups; a negative control group, a colon cancer bearing group, a quercetin-treated group and a 5-fluorouracil (5-FU)-treated group. Serum TAG72 and GAL3 levels were quantified by ELISA. Colonic Wnt5a and Axin-1 gene expression was estimated by PCR. In addition, colonic tissues were subjected to immunohistochemical examination of Bax expression and histological investigation of histopathological alterations. Results: Quercetin elicited significant reduction in serum TAG72 and GAL3 levels, in addition to significant suppression of colonic Wnt5a gene expression and amplification of colonic Axin-1 gene expression. Also, it caused moderate positive reaction for Bax in mucosal epithelium. Conclusion: The present research provides experimental evidence ...
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Hepatocarcinoma (HCC) and colorectal cancer (CRC) are the most malignant tumors with increasing incidences of morbidity and mortality globally.The aim of the present study was to determine in vitro antitumor activity of quercetin, gallic... more
Hepatocarcinoma (HCC) and colorectal cancer (CRC) are the most malignant tumors with increasing incidences of morbidity and mortality globally.The aim of the present study was to determine in vitro antitumor activity of quercetin, gallic acid, and ellagic acid against HCC and CRC. This was achieved through testing the effects of these compounds on HepG2 and HCT 116 cells, ranging from cell growth inhibition assay and fold dose advantage, passing by mitochondrial activity assessment, to cellular protein content measurement. The efficacies of these compounds were compared with doxorubicin in case of HepG2 cells and 5-fluorouracil in case of HCT 116 cells. The present findings indicated that in both cell types, gallic acid elicited the most pronounced anti-cancer activity and mitochondrial dysfunction compared to quercetin and ellagic acid. Extensive research is warranted to assess the precise molecular mechanisms of these natural products in bridling HCC and CRC.