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Shamkant B Badgujar
  • Mumbai, Maharashtra, India

Shamkant B Badgujar

NIRRH, Biochemistry, Post-Doc
Wound healing activity of E uphorbia nivulia Buch.-Ham. (Euphorbiaceae) was evaluated using excision wound model in albino mice by applying latex topically on the area of excised wound once a day for 23 days. Plant latex significantly... more
Wound healing activity of E uphorbia nivulia Buch.-Ham. (Euphorbiaceae) was evaluated using excision wound model in albino mice by applying latex topically on the area of excised wound once a day for 23 days. Plant latex significantly enhanced the rate of wound contraction and period of epithelialization, which is comparable to Soframycin. Latex of this plant significantly reduces the bleeding and whole blood clotting time. Phytochemical analysis revealed the presence of alkaloids, cynogenic glycosides, phenolics, tannins and saponins. The latex of Euphorbia nivulia possesses natural ingredients capable of arresting wound bleeding and accelerating wound healing properties which suggests healing potential to cure wound.
152-160<span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:=""... more
152-160<span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Investigations have been carried out on proteolytic activities of four laticiferous plants, viz. Calotropis procera (Ait.) R. Br. (<i style="mso-bidi-font-style: normal">Ruie), Carica papaya<span style="mso-bidi-font-style: italic"> Linn. (Papaya), Euphorbia nivulia Buch.-Ham. (Sabar) and Ficus carica Linn. (Anjir) using Hammerstein grade casein and skimmed milk powder. Ratio of milk clotting to proteolytic activity was highest in the latex sample of C. papaya followed by F. carica, <i style="mso-bidi-font-style: normal">E. nivulia and C. procera. The crude enzyme of Sabar, Ruie, Papaya and Anji...
Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A new cysteine protease named Nivulian-II has been purified from the latex of Euphorbia... more
Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A new cysteine protease named Nivulian-II has been purified from the latex of Euphorbia nivulia Buch.-Ham. The apparent molecular mass of Nivulian-II is 43670.846 Da (MALDI TOF/MS). Peptide mass fingerprint analysis revealed peptide matches to Maturase K (Q52ZV1 9MAGN) of Banksia quercifolia. The N-terminal sequence (DFPPNTCCCICC) showed partial homology with those of other cysteine proteinases of biological origin. This is the first paper to characterize a Nivulian-II of E. nivulia latex with respect to amino acid sequencing. 1.
To identify the potent source of protease that can be used as ideal milk clotting enzyme(s) in handmade dairy products of latex obtained from twenty one plant species belonging to Northern (Khandesh) region of Maharashtra, India, was... more
To identify the potent source of protease that can be used as ideal milk clotting enzyme(s) in handmade dairy products of latex obtained from twenty one plant species belonging to Northern (Khandesh) region of Maharashtra, India, was analyzed for caseinolytic activity, milk clotting activity, gelatinolytic activity and peroxidase activity. Highest caseinolytic activity was noted in latex of Pedilanthus
Ethnomedicinal survey was conducted in hilly area of North Maharashtra, India. Till today Bhills, Gavits, Kokanis, Mavachis, Padvis, Tadvis, Valvis and Vasaves tribes are exclusively dependent on forest. They have own system of herbal... more
Ethnomedicinal survey was conducted in hilly area of North Maharashtra, India. Till today Bhills, Gavits, Kokanis, Mavachis, Padvis, Tadvis, Valvis and Vasaves tribes are exclusively dependent on forest. They have own system of herbal medicine. Information on 19 plant species of 18 angiosperm families which are traditionally used as medicine to cure Kavil (Jaundice) was collected. The plant part used, traditional preparation and doses for administration are given in the present paper.
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Foeniculum vulgare Mill commonly called fennel has been used in traditional medicine for a wide range of... more
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Foeniculum vulgare Mill commonly called fennel has been used in traditional medicine for a wide range of ailments related to digestive, endocrine, reproductive, and respiratory systems. Additionally, it is also used as a galactagogue agent for lactating mothers. The review aims to gather the fragmented information available in the literature regarding morphology, ethnomedicinal applications, phytochemistry, pharmacology, and toxicology of Foeniculum vulgare. It also compiles available scientific evidence for the ethnobotanical claims and to identify gaps required to be filled by future research. Findings based on their traditional uses and scientific evaluation indicates that Foeniculum vulgare remains to be the most widely used herbal plant. It has been used for more than forty types of disorders. Phytochemical studies have shown the presence of numer...
Analyses were carried out on latex obtained from twenty one plant species belonging to Khandesh region of Maharashtra, India, for the presence of possible secondary metabolites, namely alkaloids, flavonoids, terpenoids, cynogenic... more
Analyses were carried out on latex obtained from twenty one plant species belonging to Khandesh region of Maharashtra, India, for the presence of possible secondary metabolites, namely alkaloids, flavonoids, terpenoids, cynogenic glycosides, phenolics, tannins and saponins. Phenolic compounds were found in all latex samples except in Ipomoea carnea Jacq. Seventy six percent species of laticiferous plants contain alkaloid. Terpenoids were found in the latex of Carica papaya L. and Manilkara zapota (L.) P. van Royen only however, remaining plants were devoid. Cynogenic glycosides and tannins were detected in 52 % and 48 % of latex samples respectively. Flavonoids and saponins were detected in equal number of latex samples i.e. 38 %. Out of this, four species of Apocynaceae and single species of Euphorbiaceae, Moraceae, Carricaceae, Convolvulaceae etc. showed flavonoid. While three species of Euphorbiaceae, two species of Apocynaceae and Moraceae and single species of Asclepiadaceae sh...
To identify the potent source of protease that can be used as ideal milk clotting enzyme(s) in handmade dairy products of latex obtained from twenty one plant species belonging to Northern (Khandesh) region of Maharashtra, India, was... more
To identify the potent source of protease that can be used as ideal milk clotting enzyme(s) in handmade dairy products of latex obtained from twenty one plant species belonging to Northern (Khandesh) region of Maharashtra, India, was analyzed for caseinolytic activity, milk clotting activity, gelatinolytic activity and peroxidase activity. Highest caseinolytic activity was noted in latex of Pedilanthus tithymaloides (L.) Poit., followed by Ficus carica L., Calotropis gigantea (L.) R. Br. and Carica papaya L. Moderate caseinolytic activity was seen in Euphorbia milii Desmoul., Ficus hispida L.f. and Calotropis procera (Ait.) R. Br., and remaining plants possess less activity. Milk clotting activity was found highest in stem latex of Euphorbia nivulia L., followed by Carica papaya L., Ficus carica L., Calotropis procera (Ait.) R. Br., and Tabernaemontana divaricata (L.) R. Br. Six plants showed moderate milk clotting activity, however, such activity was not noticed in remaining six pl...
Extracellular alkaline protease produced by an isolate fromsoil was preliminarily identified to be Bacillus subtilis. Studies on submerged fermentation revealed that maximum level i.e. 321 µg/ml/min of protease production was observed... more
Extracellular alkaline protease produced by an isolate fromsoil was preliminarily identified to be Bacillus subtilis. Studies on submerged fermentation revealed that maximum level i.e. 321 µg/ml/min of protease production was observed during early stationary phase. Optimization of fermentation mediumfor characterization of protease was carried out. Optimization parameter includes incubation period, temperature, pH, substrate concentration, carbon sources, nitrogen sources and tween 80 concentrations. The optimized conditions found for protease production were 400C at pH 10 (alkaline condition), with 10%inoculums, 2 ml tween 80, 1%sucrose, 1%ammoniumchloride and 20% casein, after 72 h of incubation stimulates protease production. The protease profile of the selected isolate shows its potential for its industrial applications.
A new cysteine protease named nivulian was partially characterized from the latex of Euphorbia nivulia Buch.-Ham., a member of Euphorbiaceae family. It had good stability in the presence of local detergents viz., Tide®, Ujalla®, Nirma®... more
A new cysteine protease named nivulian was partially characterized from the latex of Euphorbia nivulia Buch.-Ham., a member of Euphorbiaceae family. It had good stability in the presence of local detergents viz., Tide®, Ujalla®, Nirma® and also Triton X-100 and Tween-80 at 370C. The protein stains (blood and egg yolk) were removed within 10 – 15 min from the test fabric (cotton) cloth by the treatment of this enzyme. Thus this application of plant origin ‘nivulianÂ’ demonstrates feasibility for inclusion in laundry detergent formulation.
Nature always stands as a golden mark to exemplify the outstanding phenomenon of symbiosis. The history of herbal medicines is as old as human civilization. Many herbal remedies have been employed in various medicinal systems for the... more
Nature always stands as a golden mark to exemplify the outstanding phenomenon of symbiosis. The history of herbal medicines is as old as human civilization. Many herbal remedies have been employed in various medicinal systems for the treatment and management of different ailments. Euphorbia nivulia Buch. - Ham. a member of Euphorbiaceae family is a wild, thorny, xerophytic, succulent plant, commonly used in fencing of the agricultural field and also in dry barren areas. It has different biological activities for the treatment of several ailments of human being. It possesses antimicrobial, wound healing, haemostatic, larvicidal, insecticidal, nematicidal and cytotoxic activity. Chemically, it contains terpenes, glycoproteins, phytoelements and phytochemicals. This article provides informative data on ethnobotanical importance and bioprospecting of Euphorbia nivulia Buch. - Ham.
Investigations have been carried out on proteolytic activities of four laticiferous plants, viz. Calotropis procera (Ait.) R. Br. (Ruie), Carica papaya Linn. (Papaya), Euphorbia nivulia Buch.-Ham. (Sabar) and Ficus carica Linn. (Anjir)... more
Investigations have been carried out on proteolytic activities of four laticiferous plants, viz. Calotropis procera (Ait.) R. Br. (Ruie), Carica papaya Linn. (Papaya), Euphorbia nivulia Buch.-Ham. (Sabar) and Ficus carica Linn. (Anjir) using Hammerstein grade casein and skimmed milk powder. Ratio of milk clotting to proteolytic activity was highest in the latex sample of C. papaya followed by F. carica, E. nivulia and C. procera. The crude enzyme of Sabar, Ruie, Papaya and Anjir latices exhibit high proteolytic activity with in a pH range from 6.0-7.8, 6.5-8.0, 6.0-7.5 and 6.0-7.0, while maximum milk clotting activity with in a pH range from 6.0-6.5, 6.0-7.0, 6.0-6.5 and 6.5-7.0, respectively. The optimum temperature of proteolytic enzymes of plant latices were recorded over the temperature range 10-80°C at pH 7.0. Whereas, the optimum temperature range of milk clotting protease(s) for milk coagulation was found in between 45-55°C. Proteolytic activity of all latices was inhibited b...
Protease plays an important role in the management of defense mechanism of living organisms. This review presents a comprehensive and exhaustive account of plant, animal and microbial enzymes with special reference to proteolytic... more
Protease plays an important role in the management of defense mechanism of living organisms. This review presents a comprehensive and exhaustive account of plant, animal and microbial enzymes with special reference to proteolytic properties. A large number of biological sources of proteases described in this review clearly demonstrate the importance of plants and microbial proteases in applied and industrial uses. Proteases have potential applications in food, dairy, detergent, leather, alcoholic beverages, brewing, meat, pharmaceutical and photographic industries. The research work on protease has been going on since seven decades but no exhaustive review on protease from last eleven years is available in the literature. This review attempts to focus on some of the difficulties observed in the earlier work and tried to find out possible solutions and bridging up this gap by introducing recent information regarding proteinases. This review provides information on diverse groups of p...
An ethnobotanical study was conducted from January 2006 to October 2008 to investigate the uses of medicinal plants for oral health care by different aborigines, such as Bhills, Gavits, Kokanis, Mavachis, Valvis, Pawras, Koknas and... more
An ethnobotanical study was conducted from January 2006 to October 2008 to investigate the uses of medicinal plants for oral health care by different aborigines, such as Bhills, Gavits, Kokanis, Mavachis, Valvis, Pawras, Koknas and Vasaves, in the Nandurbar district of Maharashtra, India. Data were collected by interviewing native people, mainly elderly – engaged in farming and stock rising activities, housewives and local traditional medicinemen of different villages. The investigation revealed that a total 20 claims were obtained as distributed in 18 genera belonging to 14 families to treat various diseases and disorders of oral cavity, particularly in tooth decay. Information about local names, plant parts and different form of preparation used were recorded and are focused in given issue. In this study most commonly used family was Euphorbiaceae followed by Moraceae, Anacardiaceae, Fabaceae, Asteraceae, Acanthaceae, Meliaceae, Asclepiadaceae, Poaceae, Lythraceae, Sapotaceae, Cuc...
A newlatex protein named nivulian-I of plant origin has been isolated and purified from the latex of Euphorbia nivulia Buch.-Ham., a member of Euphorbiaceae family; by using centrifugation, acetone precipitation and ion exchange... more
A newlatex protein named nivulian-I of plant origin has been isolated and purified from the latex of Euphorbia nivulia Buch.-Ham., a member of Euphorbiaceae family; by using centrifugation, acetone precipitation and ion exchange chromatography. This protein was characterized by sodium dodecyl sulphate polyacrylamide gel electrophoresis and mass spectroscopic analysis. Molecular mass of nivulian-I is 31486.985 Da. Peptide mass fingerprint revealed that, it fairlymatches with Tubulin alpha-1 chain (TBA1_ELEIN) of Eleusine indica (L.) Gaertn, a member of Poaceae family. This investigation infers that nivulian-I may be a novel protein of plant origin.
Abstract Background and aim The pandemic COVID-19 occurring due to novel emerging coronavirus-2019 (SARS-CoV-2) is severely affecting the worldwide public health, culture, economy and human social behaviour. Till date, there is no... more
Abstract Background and aim The pandemic COVID-19 occurring due to novel emerging coronavirus-2019 (SARS-CoV-2) is severely affecting the worldwide public health, culture, economy and human social behaviour. Till date, there is no approved medicine/treatment to cure COVID-19, whereas, vaccine development efforts are going on high priority. This review aimed to provide an overview of prior art, recent advances, vaccine designing strategies, current scenario, opportunities and challenges related to development of coronavirus vaccine. Method A literature survey was conducted using Scopus, PubMed and Google Scholar with the search key as: coronavirus vaccine, SARS vaccine, MERS vaccine and COVID-19 vaccine. Articles related to above search query were retrieved, sorted, analyzed and developed into an easy-to-understand review. Results The genome phylogenetic analysis suggested that genomic sequence of SARS-CoV-2 is almost 80% similar to that of SARS-CoV, further both these viruses bind to same host cell receptor ACE-2. Hence it is expected that, previously available literature data about coronavirus vaccine designing may play crucial role in development of rapid vaccine against COVID-19. In view of this, the present review discuss (i) existing information (from 2003 to present) about the type of vaccine, antigen, immunogenic response, animal model, route of administration, adjuvants and current scenario for designing of coronavirus vaccine (ii) potential factors and challenges related to rapid development of COVID-19 vaccine. Conclusion In conclusion, we suggest possible target sites for designing of vaccine against SARS-CoV-2 virus.
We describe a simplified approach for the purification and characterization of urinary albumin, a key biomarker currently used for understanding the onset and prognosis of microalbuminuria. Urinary albumin was purified from human urine... more
We describe a simplified approach for the purification and characterization of urinary albumin, a key biomarker currently used for understanding the onset and prognosis of microalbuminuria. Urinary albumin was purified from human urine collected from diabetic kidney disease patients by using 2-stage tangential flow filtration process and set of column chromatography steps. The relative molecular mass of urinary albumin is 66,871 Da (SYNAPT G2 High Definition Mass Spectrometry System). Isolated urinary albumin was analyzed by SDS-PAGE, Western blotting, immunoelectrophoresis, Ouchterlony double-immunodiffusion, single radial immunodiffusion, size-exclusion HPLC and peptide mass fingerprint analysis. The size-exclusion HPLC elution profile of the purified urinary albumin was similar to that of a reference form of native albumin. Peptide mass fingerprint analysis of the purified urinary albumin yielded peptides that partially matched with known sequence of ALBU_HUMAN (P02768). This is the first report of purification and validation of immunochemically reactive form of urinary albumin from a large volume of urine of diabetic kidney disease patients. In this purification approach, the cost of the purified albumin is significantly lower.
Human thyroid peroxidase (hTPO) has been secretory expressed in AD293 mammalian cells. cDNA sequence of 'Gluc' (Gaussia luciferase) protein from Gaussia princeps was incorporated at the amino terminal of hTPO gene for secretion of... more
Human thyroid peroxidase (hTPO) has been secretory expressed in AD293 mammalian cells. cDNA sequence of 'Gluc' (Gaussia luciferase) protein from Gaussia princeps was incorporated at the amino terminal of hTPO gene for secretion of targeted protein outside the mammalian cells. Augmentation of TPO clone in serum free mediums was investigated and a simplified purification procedure of hTPO has been reported here. Purified hTPO was further analyzed by SDS-PAGE and immunoblotting (western blotting). The relative molecular mass of hTPO was found to be 105kDa. This is the first report with respect to cost effective and simplified purification approach to get highest yield and purity of recombinant hTPO.
Cancer antigen 15-3 (CA15-3) is a key biomarker, currently used for understanding the onset and prognosis of breast cancer. In present investigation, CA15-3 has been purified from the culture supernatant of breast cancer T47-D cell line... more
Cancer antigen 15-3 (CA15-3) is a key biomarker, currently used for understanding the onset and prognosis of breast cancer. In present investigation, CA15-3 has been purified from the culture supernatant of breast cancer T47-D cell line with 76% yield and 3350 fold purification. Isolated CA15-3 was analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting (western blotting), chemiluminescence immunoassay (CLIA) and Fourier-transform infrared spectroscopy (FTIR). CA15-3 is a monomeric protein with an apparent molecular mass in between ∼250-350kDa. The FTIR spectroscopy revealed similar profiles of T47-D derived CA15-3 and commercially available CA15-3 protein. With the easy availability of T47-D cell line and a simple purification approach described here will support for the large scale production of CA15-3 to be used for various clinical and diagnostic applications.
We describe a chromatographic approach for the purification of urinary free light chains (FLCs) viz., lambda free light chains (λ-FLCs) and kappa free light chains (κ-FLCs). Isolated urinary FLCs were analyzed by SDS-PAGE, immunoblotting... more
We describe a chromatographic approach for the purification of urinary free light chains (FLCs) viz., lambda free light chains (λ-FLCs) and kappa free light chains (κ-FLCs). Isolated urinary FLCs were analyzed by SDS-PAGE, immunoblotting and mass spectrometry (MS). The relative molecular masses of λ-FLC and κ-FLC are 22,933.397 and 23,544.336Da respectively. Moreover, dimer forms of each FLC were also detected in mass spectrum which corresponds to 45,737.747 and 47,348.028Da respectively for λ-FLCs and κ-FLCs. Peptide mass fingerprint analysis of the purified λ-FLCs and κ-FLCs has yielded peptides that partially match with known light chain sequences viz., gi|218783338 and gi|48475432 respectively. The tryptic digestion profile of isolated FLCs infers the exclusive nature of them and they may be additive molecules in the dictionary of urinary proteins. This is the first report of characterization and validation of FLCs from large volume samples by peptide sequencing. This simple and...
This investigation reports a simplified approach for the purification of urinary siderocalin known as neutrophil gelatinase-associated lipocalin (NGAL). Urinary NGAL was purified by tangential flow filtration and ion exchange... more
This investigation reports a simplified approach for the purification of urinary siderocalin known as neutrophil gelatinase-associated lipocalin (NGAL). Urinary NGAL was purified by tangential flow filtration and ion exchange chromatography. Isolated NGAL was analyzed by SDS-PAGE, immunoblotting and mass spectrometry (MS). The relative molecular mass of NGAL is 23674Da. Peptide mass fingerprinting of the purified NGAL yielded peptides that partially matched with known sequence of P80188 (NGAL_HUMAN). The tryptic digestion profile of isolated NGAL infers that it may be unique and additive molecule in the dictionary of urinary proteins. This is the first report of purification and validation of urinary NGAL from large volume sample by using tangential flow filtration and peptide sequencing respectively. This cost-effective and simplified approach to purification of NGAL, together with the easy availability of urine sample makes the large-scale production of NGAL possible, allowing exploration of various bioclinical as well as biodiagnostic applications.
The prevalence of Ulcerative Colitis (UC), once thought to be negligible, has increases exponentially in the Indian population. The development of novel, cost effective and time efficient Indirect Immunofluorescence (IIF) based assay for... more
The prevalence of Ulcerative Colitis (UC), once thought to be negligible, has increases exponentially in the Indian population. The development of novel, cost effective and time efficient Indirect Immunofluorescence (IIF) based assay for detection of anti-neutrophil cytoplasmic antibodies (ANCA) and diagnosis of UC in the Indian population is discussed. A novel IIF based assay was developed using intact nuclei from human neutrophils to detect atypical p-ANCA in patients suffering from UC. Sera from 45 patients diagnosed with UC, 45 healthy controls and one related disease control were tested using a novel UC-ANCA assay and validated by commercially available ANCA IIF assay. Prevalence of ANCA amongst UC patients in the Indian population was determined. Atypical p-ANCA was detected in 86.6% of the patients using the UC-ANCA assay as compared to 71.1% using the commercial ANCA assay. The validation of UC-ANCA assay with a commercially available ANCA IIF assay resulted in higher sensitivity. The UC-ANCA assay proved to be not only enhanced in terms of performance but also comparatively economical and rapid. The novel UC-ANCA assay may prove to be very useful in identification and differentiation of UC patients from typical ANCA positive subjects suffering from other autoimmune diseases at one tenth the cost of clinically available ANCA IIF tests which will immensely benefit the cost constrained diagnostic field of developing countries.
We describe an analytical approach for the detection and verification of glycosylation patterns of prostate specific antigen (PSA), a key biomarker currently used for understanding the onset and prognosis of prostate cancer. PSA has been... more
We describe an analytical approach for the detection and verification of glycosylation patterns of prostate specific antigen (PSA), a key biomarker currently used for understanding the onset and prognosis of prostate cancer. PSA has been purified from the human seminal plasma and total PSA from prostate cancer sera. PSA is a monomeric glycoprotein with an apparent molecular mass 28040.467Da, which exhibits a characteristic protease activity against casein and gelatin. Its optimal protease activity is centered on neutral pH. Peptide mass fingerprint analysis of the purified PSA has yielded peptides that partially match with known database sequences (Uniprot ID P07288). Tryptic digestion profile of isolated PSA, infer the exclusive nature of PSA and may be additive molecule in the dictionary of seminal proteins. Surface plasmon resonance and lectin immunoassay revealed direct interaction between a newly developed anti-PSA monoclonal antibody (C4E6) and PSA. A lectin based immunoassay is reported here which was achieved with the C4E6 anti-PSA antibody and biotinylated plant lectins. This investigation provides an alternative method to isolate and quantify PSA with altered glycosylation which might be seen in the prostate cancer and developing a lectin based immunoassay to detect PSA in serum of prostate cancer patients.
ABSTRACT Nature always stands as a golden mark to exemplify the outstanding phenomenon of symbiosis. The history of herbal medicines is as old as human civilization. Many herbal remedies have been employed in various medicinal systems for... more
ABSTRACT Nature always stands as a golden mark to exemplify the outstanding phenomenon of symbiosis. The history of herbal medicines is as old as human civilization. Many herbal remedies have been employed in various medicinal systems for the treatment and management of different ailments. Euphorbia nivulia Buch. – Ham. a member of Euphorbiaceae family is a wild, thorny, xerophytic, succulent plant, commonly used in fencing of the agricultural field and also in dry barren areas. It has different biological activities for the treatment of several ailments of human being. It possesses antimicrobial, wound healing, haemostatic, larvicidal, insecticidal, nematicidal and cytotoxic activity. Chemically, it contains terpenes, glycoproteins, phytoelements and phytochemicals. This article provides informative data on ethnobotanical importance and bioprospecting of Euphorbia nivulia Buch. - Ham.
An antigenic glycosylated cysteine protease has been purified from the latex of Euphorbia nivulia Buch.-Ham. It exhibits remarkable protease activity in the presence of metal ions, oxidizing agents, organic solvents, and detergents. This... more
An antigenic glycosylated cysteine protease has been purified from the latex of Euphorbia nivulia Buch.-Ham. It exhibits remarkable protease activity in the presence of metal ions, oxidizing agents, organic solvents, and detergents. This enzyme showed potential role in leather processing industry due to its dehairing activity for animal hide without hydrolyzing fibrous proteins, producing, by this way, a better quality product. The enzyme can also be used for silver recovering from X-ray plates. In addition, the stability (temperature and surfactants) and hydrolysis of blood stain data also revealed its application in detergent industries. Agriculturally, this protease finds application in biocontrol process against the infectious management of root knot nematode, Meloidogyne incognita. Biologically, it shows noticeable wound healing, haemostatic and antibacterial activity.
Investigations have been carried out on proteolytic activities of four laticiferous plants, viz. Calotropis procera (Ait.) R. Br. (Ruie), Carica papaya Linn. (Papaya), Euphorbia nivulia Buch.-Ham. (Sabar) and Ficus carica Linn. (Anjir)... more
Investigations have been carried out on proteolytic activities of four laticiferous plants, viz. Calotropis procera (Ait.) R. Br. (Ruie), Carica papaya Linn. (Papaya), Euphorbia nivulia Buch.-Ham. (Sabar) and Ficus carica Linn. (Anjir) using Hammerstein grade casein and skimmed milk powder. Ratio of milk clotting to proteolytic activity was highest in the latex sample of C. papaya followed by F. carica, E. nivulia and C. procera. The crude enzyme of Sabar, Ruie, Papaya and Anjir latices exhibit high proteolytic activity with in a pH range from 6.0-7.8, 6.5-8.0, 6.0-7.5 and 6.0-7.0, while maximum milk clotting activity with in a pH range from 6.0-6.5, 6.0-7.0, 6.0-6.5 and 6.5-7.0, respectively. The optimum temperature of proteolytic enzymes of plant latices were recorded over the temperature range 10-80°C at pH 7.0. Whereas, the optimum temperature range of milk clotting protease(s) for milk coagulation was found in between 45-55°C. Proteolytic activity of all latices was inhibited b...
Nature always stands as a golden mark to exemplify the outstanding phenomenon of symbiosis. The history of herbal medicines is as old as human civilization. Many herbal remedies have been employed in various medicinal systems for the... more
Nature always stands as a golden mark to exemplify the outstanding phenomenon of symbiosis. The history of herbal medicines is as old as human civilization. Many herbal remedies have been employed in various medicinal systems for the treatment and management of different ailments. Euphorbia nivulia Buch. – Ham. a member of Euphorbiaceae family is a wild, thorny, xerophytic, succulent plant, commonly used in fencing of the agricultural field and also in dry barren areas. It has different biological activities for the treatment of several ailments of human being. It possesses antimicrobial, wound healing, haemostatic, larvicidal, insecticidal, nematicidal and cytotoxic activity. Chemically, it contains terpenes, glycoproteins, phytoelements and phytochemicals. This article provides informative data on ethnobotanical importance and bioprospecting of Euphorbia nivulia Buch. - Ham.
In Ayurveda, the rhizome of Cyperus rotundus Linn has been reported for wide spectrum of biological activities including lactational therapy for increasing milk quantity. However, not a single report is available on validation of its... more
In Ayurveda, the rhizome of Cyperus rotundus Linn has been reported for wide spectrum of biological activities including lactational therapy for increasing milk quantity. However, not a single report is available on validation of its herbal galactagogue potentiality in literature. Thus, the present study is aimed to assess the lactogenic property of aqueous extract of Cyperus rotundus (CRE). The effect of aqueous extract of Cyperus rotundus rhizome was evaluated by measuring weight of the pups during suckling period. Quantitatively, total protein and carbohydrate contents of mammary tissue and serum prolactin and cortisol level were calculated. Histopathological analysis of mammary gland, pituitary gland, heart, liver, spleen, kidney, and ovary tissues was carried out. Acute toxicity of CRE against rat was assessed by the Hippocratic test and biochemical profile of blood serum. Oral administration of 300 and 600mg of CRE induced about 23% and 40% more milk in experimental group of animals as compared to the control group of animals. Weight gain by pups and mother rats of treated groups were significantly higher following administration of CRE as compared to that of control group. Moreover protein and carbohydrate content of mammary gland tissue were also significantly more than control group of animals. The CRE was found to stimulate the synthesis of prolactin significantly. In addition, the mammary gland tissues of experimental group showed obvious lobulo-alveolar development with milk secretion. Administration of CRE did not cause any signs or symptoms of toxicity which implied that Cyperus rotundus is toxicologically safe. This study demonstrates that the aqueous extract of Cyperus rotundus can stimulate milk production in the female rats which may be consequently effective in increasing the lactation of human too.
... Mahajan and Badgujar, 2008, Patil and Bhaskar, 2006 and Tayade and Patil, 2006).The fresh specimen of the plants were collected and identified taxonomically by the experts, matching with authentic herbarium, books on flora (Kirtikar... more
... Mahajan and Badgujar, 2008, Patil and Bhaskar, 2006 and Tayade and Patil, 2006).The fresh specimen of the plants were collected and identified taxonomically by the experts, matching with authentic herbarium, books on flora (Kirtikar and Basu, 1995, Naik, 1979, Naik, 1998 ...