Choo Hock Tan
Address: Venom Research & Toxicology Laboratory
Department of Pharmacology
Faculty of Medicine
University of Malaya
50603 Kuala Lumpur
Malaysia
Department of Pharmacology
Faculty of Medicine
University of Malaya
50603 Kuala Lumpur
Malaysia
less
InterestsView All (14)
Uploads
Papers by Choo Hock Tan
mix resulted in antiserum with wide para-specificity against elapid venoms from distant geographic areas. The antivenom prepared from this antiserum would be expected to be pan-specific and effective in treating envenomations by most elapids in many Asian countries.
Due to economies of scale, the antivenom could be produced inexpensively and save many lives. This simple strategy and procedure could be readily adapted for the production of pan-specific antisera against elapids of other continents.
investigated using reverse-phase HPLC, SDS-PAGE and high-resolution liquid chromatography-tandem mass
spectrometry. The findings revealed a minimalist profile with only 18 venom proteins. These proteins belong
to 5 toxin families: three-finger toxin (3FTx), phospholipase A2 (PLA2), cysteine-rich secretory protein (CRISP),
snake venom metalloprotease (SVMP) and L-amino acid oxidase (LAAO). The 3FTxs (3 short neurotoxins and
4 long neurotoxins) constitute 70.5% of total venom protein, 55.8% being short neurotoxins and 14.7% long
neurotoxins. The PLA2 family consists of four basic (21.4%) and three acidic (6.1%) isoforms. The minor proteins
include one CRISP (1.3%), two SVMPs (0.5%) and one LAAO (0.2%). This is the first report of the presence of long
neurotoxins, CRISP and LAAO in H. schistosus venom. The neurotoxins and the basic PLA2 are highly lethal in mice
with an intravenous median lethal dose of b0.2 μg/g. Cross-neutralization by heterologous elapid antivenoms
(Naja kaouthia monovalent antivenom and Neuro polyvalent antivenom) was moderate against the long
neurotoxin and basic PLA2, but weak against the short neurotoxin, indicating that the latter is the limiting factor
to be overcome for improving the antivenom cross-neutralization efficacy.
mix resulted in antiserum with wide para-specificity against elapid venoms from distant geographic areas. The antivenom prepared from this antiserum would be expected to be pan-specific and effective in treating envenomations by most elapids in many Asian countries.
Due to economies of scale, the antivenom could be produced inexpensively and save many lives. This simple strategy and procedure could be readily adapted for the production of pan-specific antisera against elapids of other continents.
investigated using reverse-phase HPLC, SDS-PAGE and high-resolution liquid chromatography-tandem mass
spectrometry. The findings revealed a minimalist profile with only 18 venom proteins. These proteins belong
to 5 toxin families: three-finger toxin (3FTx), phospholipase A2 (PLA2), cysteine-rich secretory protein (CRISP),
snake venom metalloprotease (SVMP) and L-amino acid oxidase (LAAO). The 3FTxs (3 short neurotoxins and
4 long neurotoxins) constitute 70.5% of total venom protein, 55.8% being short neurotoxins and 14.7% long
neurotoxins. The PLA2 family consists of four basic (21.4%) and three acidic (6.1%) isoforms. The minor proteins
include one CRISP (1.3%), two SVMPs (0.5%) and one LAAO (0.2%). This is the first report of the presence of long
neurotoxins, CRISP and LAAO in H. schistosus venom. The neurotoxins and the basic PLA2 are highly lethal in mice
with an intravenous median lethal dose of b0.2 μg/g. Cross-neutralization by heterologous elapid antivenoms
(Naja kaouthia monovalent antivenom and Neuro polyvalent antivenom) was moderate against the long
neurotoxin and basic PLA2, but weak against the short neurotoxin, indicating that the latter is the limiting factor
to be overcome for improving the antivenom cross-neutralization efficacy.