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Promoter of COR2-like gene is a stress inducible regulatory region in banana
A promoter is a crucial component in driving the expression of a transgene of interest for biotechnological applications in crop improvement and thus...
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Expression of toxic genes in Methylorubrum extorquens with a tightly repressed, cumate-inducible promoter
Methylorubrum extorquens is an important model methylotroph and has enormous potential for the development of C1-based microbial cell factories....
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Unlocking Nature’s Toolbox: glutamate-inducible recombinant protein production from the Komagatella phaffii PEPCK promoter
BackgroundKomagataella phaffii (a.k.a. Pichia pastoris) harbors a glutamate utilization pathway in which synthesis of glutamate dehydrogenase 2 and...
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Bidirectional hybrid erythritol-inducible promoter for synthetic biology in Yarrowia lipolytica
BackgroundThe oleaginous yeast Yarrowia lipolytica is increasingly used as a chassis strain for generating bioproducts. Several hybrid promoters...
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A nematode-inducible promoter can effectively drives RNAi construct to confer Meloidogyne incognita resistance in tomato
Key messageHeterologous expression of a nematode-responsive promoter in tomato successfully driven the RNAi constructs to impart root-knot nematode...
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Identification of the inducible activity in the promoter of the soybean BBI-DII gene exposed to abiotic stress or abscisic acid
The expression of the soybean Bowman–Birk proteinase isoinhibitor DII ( BBI-DII ) gene and the inducible activity of its promoter were studied under...
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Novel inducible promoter DREB1G cloned from date palm exhibits high fold expression over AtRD29 to drought and salinity stress
Right and timely expression of the stress regulatory genes is required for plants to compete against abiotic stresses; it necessitates the isolation...
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Development of constitutive and IPTG-inducible integron promoter-based expression systems for Escherichia coli and Agrobacterium tumefaciens
Broad host range (BHR) expression vector is a vital tool in molecular biology research and application. Currently, most of the plasmid vectors used...
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Overexpression of the DREB1A gene under stress-inducible promoter delays leaf senescence and improves drought tolerance in rice
Rice ( Oryza sativa ) is the major source of food as over half of the world's population relies on rice as their major source of nutrition. Drought...
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Engineering a synthetic gene circuit for high-performance inducible expression in mammalian systems
Inducible gene expression systems can be used to control the expression of a gene of interest by means of a small-molecule. One of the most common...
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Identification and validation of the wound and insect bite early inducible promoter from Arabidopsis thaliana
A wound-inducible promoter facilitates the regulated gene expression at the targeted site during the time of mechanical stress or infestation by the...
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An inducible Komagataella phaffii system for protein expression using sorbitol dehydrogenase promoter
ObjectivesThe aim of the present work was to develop a methanol-independent Komagataella phaffii ( K. phaffii ) strain using a non-methanol promoter.
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Early Blight Resistance of Transgenic Potato Plants Expressing the ProSmAMP1 Gene for Antimicrobial Peptides under the Control of a Light-Inducible Cab Promoter
AbstractThe genome of Stellaria media contains a gene family for hevein-like antimicrobial peptides, some of which are known to encode two peptides...
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An inducible CRISPRi circuit for tunable dynamic regulation of gene expression in Saccharopolyspora erythraea
Actinomyces are gram-positive bacteria known for their valuable secondary metabolites. Redirecting metabolic flux towards desired products in...
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An Erwinia amylovora inducible promoter for improvement of apple fire blight resistance
Key messagepPPO16, the first Ea-inducible promoter cloned from apple, can be a useful component of intragenic strategies to create fire blight...
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Tuning of Protein Expression by Promoter Engineering
The promoter is an essential component of an expression system since it regulates the transcriptional beginning of related genes. The optimal... -
Deep flanking sequence engineering for efficient promoter design using DeepSEED
Designing promoters with desirable properties is essential in synthetic biology. Human experts are skilled at identifying strong explicit patterns in...
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Detecting Hypoxia-Inducible Factor Levels and Activity
The hypoxia-inducible transcription factors HIF-1 and HIF-2 regulate the response to hypoxia. Both proteins are dimers of an alpha subunit and a... -
Molecular characterization of the ER stress-inducible factor CRELD2
Previously, we found by constructing various luciferase reporters that a well-conserved ATF6-binding element in the CRELD2 promoter is activated by...
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Delaying production with prokaryotic inducible expression systems
BackgroundEngineering bacteria with the purpose of optimizing the production of interesting molecules often leads to a decrease in growth due to...