ALPER BARAN

The goal of this study was to evaluate the abnormal spermatozoa rates and types of American Bronze turkeys under Turkish conditions. Semen was collected from toms by abdominal massage and pooled once a week for 10 weeks. After pooling, the semen samples were examined morphologically and mean abnormal rates and types were evaluated. A total abnormal spermatozoa rate of 17 - 0.06% was determined. Acrosome and mid-piece defects were the leading defects at 66 - 0.36%. Tail defects of 22 - 0.18% and head defects of 11 - 0.11% were the next most prevalent. In conclusion, we observed that the most common morphological defects were at the acrosome and the mid-piece, and these organelles were the most sensitive to various environmental conditions.

Ozet Calismada kullanilan 32 adet disi kopekten 16'si taze sperma, kalan 16'si ise %4 gliserollu Tris-fruktoz-sitrik asit sulandiricisinda payet yontemine gore dondurulmus sperma ile intravaginal yolla tohumlandi. Optimal tohumlama zamaninin tespitinde "Ovulasyon Test Kiti" kullanildi ve yari kantitatif saptanan progesteron degerine bakilarak (1-8 ng/ml) taze ve donmus sperma ile disiler gun asin 3 kez tohumlandi. Gebelik orani taze sperma ile 81.25, dondurulmus sperma ile %37.50 olarak saptandi ve aralarindaki fark onemli bulundu (p<0.01). Taze ve dondurulmus sperma ile dogum basina dusen ortalama yavru sayisi 4.69 ve 3.33 olarak saptandi. Sonuc olarak, disi kopeklerde taze sperma ile intravaginal suni tohumlama tekniginin, cerrahi bir uygulama veya anesteziye ihtiyac gostermemesi gibi avantajlari nedeniyle tum veteriner hekimler tarafindan uygulamada kullanilabilecegi ancak, donmus sperma ile bu yolla dusuk gebelik oranlari elde edilecegi dusunulmelidir. Anaht...

OZET Gunumuzde dondurulmus embriyolarin transferi ile ustun verim ozelliklerine sahip surulerin olusturulmasi, hastaliklarin kontrolu ve genetik materyallerin uzun sure saklanmasi mumkun olabilmektedir. Ancak donmus embriyolarda eritme sonrasi tranfer edilebilir embriyo eldesi ve gebelik oranlari istenilen duzeyde degildir. Ozellikle embriyolarin dondurulmasi sirasinda dejenerasyonlar olusmaktadir. Dejenerasyonlarin, embriyolarin kriyoprotektif maddelere maruz kalma sureleri ile toksik etkilerinden meydana geldigi dusunulmektedir. Calismada mezbahada kesilen sigirlarin ovaryumlari kullanildi. Aspirasyon yontemi ile elde edilen oositler (1290 adet) TCM-199 da 22-24 saat sureyle % 5 CO 2 , %5 O 2 , %90N 2 gaz atmosferinde 38,8 oC de in vitro olarak olgunlastirildilar. Olgun oositler IVF-TALP medyumunda 18-24 saat sureyle fertilize edildiler. Fertilizasyon sonrasi 48. saatte cleavage %67,05 (865/1290) saptandi. Embriyolar %10 FCS'li SOF medyumunda 7 gun sureyle % 5 CO 2 , %5 O 2 , ...

... Mithat EVECEN, Hatem ATALLA, Sema B‹RLER, Alper BARAN, Kemal AK ‹stanbul Üniversitesi, Veteriner Fakültesi, Dölerme ve Sun'i Tohumlama ... Sonuç olarak, kedi oositlerinin in vitro olgunlaflt›r›lmas›nda SOS medyumunun Ham's F-10 medyumundan daha baflar›l› oldu¤u ...

Bu çalışmada kısraklarda östrüsün; rektal palpasyon, ultrasonografi ve hızlı kan-progesteron testi yardımıyla karşılaştırmalı olarak tespiti amaçlandı. Materyal olarak yaşları 5-15 arasında değişen saf ve yarım kan 20 İngiliz kısrak kullanıldı. Östrüs tespiti, deneme aygırı muayenesinden sonra rektal palpasyon ve ultrasonografi yardımıyla gerçekleştirildi. Follikül çapları Östrüs boyunca rektal palpasyon ve ultrason kullanılarak östrüsün birinci gününden ovulasyon tespitine kadar gün aşırı ölçüldü. Follikül çapı ölçümlerini takiben kısraklardan gün aşırı olmak koşuluyla östrüsün birinci gününden ovulasyona kadar kan örnekleri alındı. Alınan örnekler 5000 devirde 20 dakika süreyle santrifüj edilerek serumlar elde edildi. Elde edilen serum iki eşit kısma ayrıldı. Birinci kısım serumda EIA (hızlı test), ikinci kusim serumda ise RIA yöntemine göre progesteron tayini yapıldı. Rektal palpasyon ve ultrasonografik ölçümler arasındaki korelasyon (r=0.92) önemli bulundu (p<0.01). Serum pro...

ABSTRACT The aim of the study was to determine the effects of gestation type (single or twin) and parity (primiparous or pluriparous), on uterus involution period and on parameters that are related with uterus involution such as; caruncular diameter (CD), uterine horn diameter (UHD), uterine endometrial wall tickness (UEWT) and presence of vaginal discharge in Saanen does, ultrasonographically. Totally 20 Saanen does were used for the study. Ten of them delivered twin offsprings (twin group), the other 10 does delivered single offspring (single group). The does were further categorized as either primiparous (n=7) or pluriparous (n=13). Ultrasonographic examinations were performed at postpartum (pp) 1st, 2nd, 4th, 7th, 10th, 13th, 19th, 25th, 28th days, except in three does ultrasonography was continued till 29th day of pp in which their uterine involution completed at 29th day pp. During ultrasonographic examinations CD, UHD, UEWT were measured on the most thickest uterine horn and were continued always on the same uterine horn; presence of vaginal discharge were recorded in these days, too. Two-way ANOVA and chi-square tests were used for statistical analysis of data. Gestation type and parity had no significant influence on CD, UHD and involution period except for CD on day 13, in Saanen does. However, uterine endometrial wall thickness in days 10, 13 and 19 were higher in twin delivered does than single ones (P<0.01). The percentage of does having vaginal discharge was higher in twin group when compared with single group at day 7 postpartum (pp), (P<0.01) and the percentage of does having vaginal discharge was higher in pluriparous group at day 7 pp (P<0.05) compared with primiparous ones. The results of the study indicate that vaginal discharge was ceased more rapidly in twin parturated and pluriparous does when compared with single parturated and primiparous does, respectively. According to these results uterine involution was detected as completed at the similar periods in twin and single delivering, and in primiparous and pluriparous Saanen does.

... (LH pik), C3: Donuk Mavi=2.5-8 ng/ml., C4: Beyaz= >8 ng/ml. Çalışmada optimal tohumlama zamanı ... (Doktora Tezi), Ankara. 8. Keskin, O., Akçay, E., Kaya, M., Yurdaydın, N., Tekin, N., Gürcan, S. (1996): Değişik ırktan köpeklerde sun'i tohumlama çalışmaları. Lalahan Hay. Arş. ...

The aim of this study was to determine the effect of GnRH + PGF2a and hCG + PGF2a applications on oestrus and fertility in dairy cows postpartum between days 35 and 45. In this study, 117 healthy, 3-7-year-old Holstein dairy cows from the same farm under similar husbandry conditions were used and they were in postpartum days between 35 and 45. Cows were divided equally into three groups randomly. Group I (n = 39): on day 0, 2000 IU hCG IM (Pregnyl¨, Organon) was given, on day 7, 500 µg Cloprostenol Na IM (Estrumate¨, DÜF), and on day 9, 12.6 µg Buserelin acetate IM (Receptal¨, Hoechst). Group II (n = 39): on day 0, 12.6 µg Buserelin acetate IM (Receptal¨, Hoechst) was given, on day 7, 500 µg Cloprostenol Na IM (Estrumate¨, DÜF), and on day 9, 2000 IU hCG IM (Pregnyl¨, Organon). Group III (n = 39): they received 500 µg Cloprostenol IM (Estrumate¨, DÜF) twice, at 11-d intervals. The diameters of the follicules in ovaria were measured in the cows in groups I and II on day 9, and in the...

The aim of this study was to determine the effects of FSH and LH hormones on in vitro development of cat oocytes. The ovaries from spayed cats were brought to the laboratory in +4 ¡C PBS solution two hours after being collected. The oocytes were recovered by slicing of ovaries and were separated into two groups: Group 1 (Control); TCM 199 + 5% FCS + 2.2 g/l NaHCO3 + 0.23 mM Na Pyruvate; and Group 2 (Treatment); TCM 199 + 5% FCS + 2.2 g/l NaHCO3 + 0.23mM Na Pyruvate + FSH (Sigma F- 2293) (1 µg/ml) + LH (Sigma L-5269) (1 µg/ml). Oocytes were matured for 48 hours at 38.5 ¡C and 5% CO2 in a humidified atmosphere. At the end of this period oocytes were fixed, stained and evaluated for maturation criteria by phase-contrast microscope at x400 magnification. Data were analyzed statistically by Students t - test. A total of 428 oocytes (Control: 203; Treatment: 225) were used. Eleven of the control group oocytes were at M I (5.4%), 2 (0.98%) were at anaphase I and 2 (0.98%) were at M II stag...

The types and rates of acrosome and other (head, mid-piece and tail) abnormal spermatozoon types were determined by the Spermac¨ staining technique. Semen from 5 stray tom cats under the same management conditions was used. Semen collection was performed under general anesthesia by electro-ejaculator once a week for 5 weeks. After ejaculation the semen was diluted by 100 µl of 0.9% NaCl solution and stained with Spermac¨ stain for morphological evaluation. The morphological criteria were acrosome, other (head, mid-piece and tail) and total morphological defect rates, and were 9.20 ± 2.55%, 9.20 ± 3.49% and 18.40 ± 4.79%, respectively. It was concluded at the end of the study that the Spermac¨ staining technique could provide a detailed observation of cat spermatozoa, especially the acrosomal region, and could be employed in the determination of acrosomal defects.

zet: Köpek oositlerinin in vitro olgunlaflt›r›lmas› için medyuma kat›lan protein kaynaklar›n›n etkisinin incelendi¤i bu çal›flmada, k›s›rlaflt›r›lm›fl köpeklerin ovaryumlar› kullan›ld›. Ovaryumlar 38 ºC s›cakl›ktaki PBS solüsyonu içerisinde, k›s›rlaflt›rma ifllemini müteakip en geç bir saat içerisinde laboratuara getirildi. Elde edilen oositler üç gruba ayr›larak, % 5 CO 2 ve yüksek nem' in sa¤land›¤› 38,5º C s›cakl›ktaki inkübatörde 72 saat olgunlaflmaya b›rak›ld›. Grup 1 (Kontrol): TCM -199 medyumu, Grup 2 (BSA): % 0,3 BSA katk›l› TCM –199 medyumu, Grup 3 (FCS): % 5 FCS katk›l› TCM –199 medyumu. Olgunlaflma süresinin sonunda, oositlerin tümü pipetlenerek kumulus hücrelerinden ar›nd›r›ld› ve 1:3 asetik asit:etanol ile 48 saat fikse edildi. Oositler % 2 aseto-orsein ile boyand›ktan sonra olgunlaflma kriterleri aç›s›ndan faz-kontrast mikroskopta x400 büyütmede incelendi. Toplam 624 oositin kullan›ld›¤› çal›flmada kontrol, BSA ve FCS gruplar›nda s›ras›yla ; %16,58 (34/205), %37,08...

Praca oryginalna Original paper 2,4-Dichlorophenol (2,4-DCP) is used in the produc-tion of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), and it is one of the abundant chlorophenols in the aquatic environment. These pollutants, which are widely distributed in nature, have detrimental biologi-cal effects, including chronic toxicity, mutagenicity and carcinogenicity (18). 2,4-DCP is also utilized in the manufacture of methylated chlorophenols that are used for mothproofing, in antiseptics and disinfectants (1). Because of this great ubiquity, 2,4-DCP, together with other chlorophenols, was listed as early as 1978, among 129 priority pollutants in 65 classes by the US Envi-ronmental Protection Agency (10). In recent years, there has been increasing interest in the toxicology of the male reproductive system. Con-troversial reports on reproductive disorders associated with the exposure of males to occupational toxicants has partly triggered this interest (2, 6, 13). Increasing rep...

The objective of the current study was to investigate the eff ects of the addition of oviduct co-culture to synthetic oviduct fl uid (SOF) and Ménézo B2 media used in an in vitro study of cows, and the eff ects of diff erent gas atmospheres (5% CO2 or 5% CO2, 5% O2, and 90% N2) on in vitro cultures. Oocytes obtained through aspiration from the ovaries of slaughtered Holstein cows were washed and cultured within a TCM-199 maturation medium at 38.8 °C for 23 h. Th en the matured oocytes and thawed semen, prepared in accordance with the swim-up method, were incubated for fertilization within in vitro fertilization (IVF)-Tyrode’s albumin lactate pyruvate (TALP) medium with an atmosphere including the combination of 5% CO2, 5% O2, and 90% N2 gas, at 38.8 °C for 18-24 h. Fertilized oocytes were distributed into 3 main groups: Group I: B2 (5% CO2), Group II: B2 medium (5% CO2, 5% O2, and 90% N2), and Group III: SOF (5% CO2, 5% O2, and 90% N2), and each group was divided into 2 subgroups, w...

Oxidative stress during in vitro culture leads to defects in development of gametes and embryos. Several antioxidants such as cysteamine, L-ascorbic acid, beta mercaptoethanol, cysteine, glutathione, proteins, vitamins have been used to supplement culture media to counter the oxidative stress. This study was conducted to detect the effect of adding cysteamine to the maturation medium to subsequent cleavage rates of sheep embryos. Totally 604 ovaries were obtained by ten replica and 2060 oocytes were collected. The cumulus oocyte complexes were recovered by the slicing method. A total of 1818 selected oocytes were divided into two groups and used for maturation (88.25%). The first group was created as supplemented with cysteamine (Group A) and second group (Group B, control) without cysteamine in TCM-199. The two groups were incubated for 24 h at 38.8 °C in an atmosphere of 5% CO2 in humidified air for in vitro maturation (IVM). After IVM, oocytes were fertilized with 50 x 107 / mL f...

The aim of this study was to investigate the post-thaw motility and morphological defect rates of cat semen frozen with various extenders, and pregnancy rates aft er intrauterine insemination (IUI). Cats, 2 males and 14 queens, 2 to 3 years of age, and under the same managemental conditions were used. Semen was collected by means of an electroejaculator under general anesthesia. Based on spermatological traits, semen samples presumed suitable for freezing were extended with either tris-fructose-citric acid (EY-TFC) containing 20% egg yolk, or skimmed milk-glucose-taurine (EY-SMGT) containing 10% egg yolk extender, and frozen in 0.25 mL straws. For the extenders, post-thaw motility and morphological abnormality values were found to be effi cient for IUI and no statistical signifi cance was observed between the values of the extender groups. To achieve ovulation, queens showing behavioral estrus received 250 IU of hCG via IM route, 30 h prior to IUI. Under general anesthesia, queens w...

The aim of this study was to determine the effects of 2 different transport temperatures on the in vitro maturation of oocytes collected from frozen-thawed sheep ovaries. Sheep ovaries were transferred into saline at temperatures of 4 °C and 32 °C. After the 2 experimental groups (A: fresh cortex, B: frozen-thawed cortex) were formed, each group was divided into 2 subgroups (group A1: 4 °C, group A2: 32 °C [control]; group B1: 4 °C, group B2: 32 °C). The cortexes were dissected into slices 1-3 mm thick and pieces of 0.5 cm2. For groups B1 and B2, 1-2 cortex pieces were placed in cryogenic vials containing 1 mL of freezing medium modified with Earle’s salts (TCM-199) and supplemented with 10% fetal calf serum (FCS) (FCS + 2.5 M ethylene glycol + 0.1 M sucrose). The vials were then cooled to −7 °C at 2 °C/min and held at −7 °C for 10 min for manual seeding. The temperature was then lowered by −0.3 °C/min to −35 °C and thereafter by −10 °C/min to −75 °C. Vials were plunged into −196 °C...

Abstract: The aim of the present study was to investigate the effects of gonadotrophin and Bovine Serum Albumin (BSA) supplemented Synthetic Oviduct Fluid (SOF) and Ham's F-10 media on the in vitro maturation of cat oocytes. Oocytes collected from spayed stray ...

Abstract: The objectives of this study were to (i) compare the effects of two staining techniques and one fixative solution on the morphological features of ram spermatozoa and (ii) evaluate the morphological features of dead/live spermatozoa by using Eosin-Nigrosin ...