Both those who provide services and those for whom the services are intended are responsible for ... more Both those who provide services and those for whom the services are intended are responsible for the barriers to the spread and adoption of family planning. The barriers on the consumers side are easy to identify but difficult to overcome. The major bottleneck appears to be political indifference or opposition and inappropriate provision of services rather than the reluctance of the individual consumer. From the point of view of those professionals involved in family planning the focus of attention must be directed to the barriers and bottlenecks which obstruct the provision and extension of services now. The demand for family planning exceeds services in many areas of the world and there is a large unmet market for contraceptive services. It is not a question of manipulating peoples desires but of meeting existing demand. Those providing services should look long and hard at available services to determine whether they meet consumer needs and perceptions. Both democratically electe...
Corynebacterium diphtheriae is the etiological agent of diphtheria; an acute toxin-mediated infec... more Corynebacterium diphtheriae is the etiological agent of diphtheria; an acute toxin-mediated infection of the upper respiratory tract and skin. The toxin gene is encoded on a temperate bacteriophage, which during lysogeny is capable of toxin production. In this chapter, we will summarise current knowledge regarding corynephage, focusing on the lifecycle and biology of the toxin-carrying corynephages that are known to convert pathogenic corynebacteria, and discuss the insights recent genome corynebacterial sequencing has given us.
Rhodococcus equi ("Prescottella equi") is a pathogenic actinomycete primarily infecting... more Rhodococcus equi ("Prescottella equi") is a pathogenic actinomycete primarily infecting horses but has emerged as an opportunistic human pathogen. We have sequenced the genome of the type strain of this species, R. equi strain C7(T) , and compared the genome with that of another foal isolate 103S and of a human isolate ATCC 33707. The R. equi strains are closely related to each other and yet distantly related to other rhodococci and Nocardia brasiliensis. The comparison of gene contents among R. equi strains revealed minor differences that could be associated with host adaptation from foals to humans, including the presence of a paa operon in the human isolate which is potentially involved in pathogenesis. This article is protected by copyright. All rights reserved.
We report the draft genome sequence of the human pathogen Streptomyces somaliensis (DSM 40738), a... more We report the draft genome sequence of the human pathogen Streptomyces somaliensis (DSM 40738), a pathogen within a genus of largely saprophytic organisms. S. somaliensis causes severe and debilitating deep tissue and bone infections. The genome sequence is deposited in DDBJ/EMBL/GenBank with the accession number AJJM01000000.
In the era when large whole genome bacterial datasets are generated routinely, rapid and accurate... more In the era when large whole genome bacterial datasets are generated routinely, rapid and accurate molecular systematics is becoming increasingly important. However, 16S ribosomal RNA sequencing does not always offer sufficient resolution to discriminate between closely related genera. The SsgA-like proteins are developmental regulatory proteins in sporulating actinomycetes, whereby SsgB actively recruits FtsZ during sporulation-specific cell division. Here, we present a novel method to classify actinomycetes, based on the extraordinary way the SsgA and SsgB proteins are conserved. The almost complete conservation of the SsgB amino acid (aa) sequence between members of the same genus and its high divergence between even closely related genera provides high-quality data for the classification of morphologically complex actinomycetes. Our analysis validates Kitasatospora as a sister genus to Streptomyces in the family Streptomycetaceae and suggests that Micromonospora, Salinispora and ...
We report the draft genome of Streptomyces rimosus (ATCC 10970), a soil isolate that produces oxy... more We report the draft genome of Streptomyces rimosus (ATCC 10970), a soil isolate that produces oxytetracycline, a commercially important and clinically useful antibiotic.
Rhodococcus equi ('Prescottella equi') is a pathogenic actinomycete primarily infecting h... more Rhodococcus equi ('Prescottella equi') is a pathogenic actinomycete primarily infecting horses but has emerged as an opportunistic human pathogen. We have sequenced the genome of the type strain of this species, R. equi strain C7(T) , and compared the genome with that of another foal isolate 103S and of a human isolate ATCC 33707. The R. equi strains are closely related to each other and yet distantly related to other rhodococci and Nocardia brasiliensis. The comparison of gene contents among R. equi strains revealed minor differences that could be associated with host adaptation from foals to humans, including the presence of a paa operon in the human isolate, which is potentially involved in pathogenesis.
Rhodococcus defluvii strain Ca11(T) was isolated from a bioreactor involved in extensive phosphor... more Rhodococcus defluvii strain Ca11(T) was isolated from a bioreactor involved in extensive phosphorus removal. We have sequenced the whole genome of this strain, and our comparative genomic and phylogenetic analyses confirm its close relatedness with Rhodococcus equi (Rhodococcus hoagii) strains, which share >80 % of the gene content. The R. equi virulence plasmid is absent though most of the chromosomal R. equi virulence-associated genes are present in R. defluvii Ca11(T). These data suggest that although R. defluvii is an environmental organism, it has the potential to colonize animal hosts.
Both those who provide services and those for whom the services are intended are responsible for ... more Both those who provide services and those for whom the services are intended are responsible for the barriers to the spread and adoption of family planning. The barriers on the consumers side are easy to identify but difficult to overcome. The major bottleneck appears to be political indifference or opposition and inappropriate provision of services rather than the reluctance of the individual consumer. From the point of view of those professionals involved in family planning the focus of attention must be directed to the barriers and bottlenecks which obstruct the provision and extension of services now. The demand for family planning exceeds services in many areas of the world and there is a large unmet market for contraceptive services. It is not a question of manipulating peoples desires but of meeting existing demand. Those providing services should look long and hard at available services to determine whether they meet consumer needs and perceptions. Both democratically electe...
Corynebacterium diphtheriae is the etiological agent of diphtheria; an acute toxin-mediated infec... more Corynebacterium diphtheriae is the etiological agent of diphtheria; an acute toxin-mediated infection of the upper respiratory tract and skin. The toxin gene is encoded on a temperate bacteriophage, which during lysogeny is capable of toxin production. In this chapter, we will summarise current knowledge regarding corynephage, focusing on the lifecycle and biology of the toxin-carrying corynephages that are known to convert pathogenic corynebacteria, and discuss the insights recent genome corynebacterial sequencing has given us.
Rhodococcus equi ("Prescottella equi") is a pathogenic actinomycete primarily infecting... more Rhodococcus equi ("Prescottella equi") is a pathogenic actinomycete primarily infecting horses but has emerged as an opportunistic human pathogen. We have sequenced the genome of the type strain of this species, R. equi strain C7(T) , and compared the genome with that of another foal isolate 103S and of a human isolate ATCC 33707. The R. equi strains are closely related to each other and yet distantly related to other rhodococci and Nocardia brasiliensis. The comparison of gene contents among R. equi strains revealed minor differences that could be associated with host adaptation from foals to humans, including the presence of a paa operon in the human isolate which is potentially involved in pathogenesis. This article is protected by copyright. All rights reserved.
We report the draft genome sequence of the human pathogen Streptomyces somaliensis (DSM 40738), a... more We report the draft genome sequence of the human pathogen Streptomyces somaliensis (DSM 40738), a pathogen within a genus of largely saprophytic organisms. S. somaliensis causes severe and debilitating deep tissue and bone infections. The genome sequence is deposited in DDBJ/EMBL/GenBank with the accession number AJJM01000000.
In the era when large whole genome bacterial datasets are generated routinely, rapid and accurate... more In the era when large whole genome bacterial datasets are generated routinely, rapid and accurate molecular systematics is becoming increasingly important. However, 16S ribosomal RNA sequencing does not always offer sufficient resolution to discriminate between closely related genera. The SsgA-like proteins are developmental regulatory proteins in sporulating actinomycetes, whereby SsgB actively recruits FtsZ during sporulation-specific cell division. Here, we present a novel method to classify actinomycetes, based on the extraordinary way the SsgA and SsgB proteins are conserved. The almost complete conservation of the SsgB amino acid (aa) sequence between members of the same genus and its high divergence between even closely related genera provides high-quality data for the classification of morphologically complex actinomycetes. Our analysis validates Kitasatospora as a sister genus to Streptomyces in the family Streptomycetaceae and suggests that Micromonospora, Salinispora and ...
We report the draft genome of Streptomyces rimosus (ATCC 10970), a soil isolate that produces oxy... more We report the draft genome of Streptomyces rimosus (ATCC 10970), a soil isolate that produces oxytetracycline, a commercially important and clinically useful antibiotic.
Rhodococcus equi ('Prescottella equi') is a pathogenic actinomycete primarily infecting h... more Rhodococcus equi ('Prescottella equi') is a pathogenic actinomycete primarily infecting horses but has emerged as an opportunistic human pathogen. We have sequenced the genome of the type strain of this species, R. equi strain C7(T) , and compared the genome with that of another foal isolate 103S and of a human isolate ATCC 33707. The R. equi strains are closely related to each other and yet distantly related to other rhodococci and Nocardia brasiliensis. The comparison of gene contents among R. equi strains revealed minor differences that could be associated with host adaptation from foals to humans, including the presence of a paa operon in the human isolate, which is potentially involved in pathogenesis.
Rhodococcus defluvii strain Ca11(T) was isolated from a bioreactor involved in extensive phosphor... more Rhodococcus defluvii strain Ca11(T) was isolated from a bioreactor involved in extensive phosphorus removal. We have sequenced the whole genome of this strain, and our comparative genomic and phylogenetic analyses confirm its close relatedness with Rhodococcus equi (Rhodococcus hoagii) strains, which share >80 % of the gene content. The R. equi virulence plasmid is absent though most of the chromosomal R. equi virulence-associated genes are present in R. defluvii Ca11(T). These data suggest that although R. defluvii is an environmental organism, it has the potential to colonize animal hosts.
Uploads