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Research Interests: Business and Food Chain
The aim of this in vitro study was to examine the dose-dependent effects of iron as a potential endocrine disruptor in relation to the release of sexual steroid hormones by a human adrenocortical carcinoma (NCI-H295R) cell line. The cells... more
The aim of this in vitro study was to examine the dose-dependent effects of iron as a potential endocrine disruptor in relation to the release of sexual steroid hormones by a human adrenocortical carcinoma (NCI-H295R) cell line. The cells were exposed to different concentrations (3.90, 62.50, 250, 500, 1000 μM) of FeSO4.7H2O and compared with the control group (culture medium without FeSO4.7H2O). Cell viability was measured by the metabolic activity assay. Quantification of sexual steroid production was performed by enzyme-linked immunosorbent assay. Following 48 h culture of the cells in the presence of FeSO4.7H2O, significantly (P < 0.001) increased production of progesterone was observed at the lowest concentration (3.90 μM) of FeSO4.7H2O, whereas the lowest release of progesterone by NCIH295R cells was noted after addition of 1000 μM of FeSO4.7H2O, which did not elicit cytotoxic action (P > 0.05). Testosterone production was substantially increased at the concentrations ≤ ...
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The purpose of this study was to monitor concentration of selected biological and risk elements in testes and later epididymal spermatozoa motility of wild boars (Sus scrofa scrofa) as well as their association. Wild boars were hunted in... more
The purpose of this study was to monitor concentration of selected biological and risk elements in testes and later epididymal spermatozoa motility of wild boars (Sus scrofa scrofa) as well as their association. Wild boars were hunted in region Žuhračka - Levice branch plant, Slovak Republic. Testes were surgically removed post-mortem and were transported to the laboratory. Spermatozoa were obtained by dissecting the distal portion of the cauda epididymis and were analysed by Computer assisted semen analyzer (CASA) system. Concentration of elements were measured by inductively-coupled plasma optical emission spectrometry (ICP-OES) and by cold vapor absorption spectrometer (MA-3 Solo Mercury Analyzer). Total spermatozoa motility was at the level of 44.29% and progressive motility was 18.47%. Concentration of elements in testes was in following order: K > Na > Mg > Ca > Fe > Zn > Al > Cu > Se > Mn > As > Cr > Pb > Mo > Sr > Ni > Ba > ...
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Various studies have shown that the reproductive organs are highly sensitive to toxic elements found in the environment. Due to technological progress, the use of nanoparticles has become more common nowadays. Nanoparticles are used for... more
Various studies have shown that the reproductive organs are highly sensitive to toxic elements found in the environment. Due to technological progress, the use of nanoparticles has become more common nowadays. Nanoparticles are used for drug delivery because their dimensions allow them to circulate throughout the body and enter directly into the cell. Antimicrobial properties are increasingly used in the manufacture of medical devices, textiles, food packaging, cosmetics, and other consumer products. Nanoparticles provide several benefits, but aspects related to their effects on living organisms and the environment are not well known. This review summarizes current in vivo, and in vitro animal studies focused on the evaluation of toxicity of selected metal nanoparticles (Ag, ZnO, TiO2) on male and female reproductive health. It can be concluded that higher concentrations of metal nanoparticles in the male reproductive system can cause a decrease in spermatozoa motility, viability an...
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The objective of the present study was to investigate semen quality of infertile smokers and non-smokers including Benzo[a]Pyrene-7,8-diol-9,10 epoxide (BPDE)-DNA adduct formation and acrosome status of sperm cells. Semen quality... more
The objective of the present study was to investigate semen quality of infertile smokers and non-smokers including Benzo[a]Pyrene-7,8-diol-9,10 epoxide (BPDE)-DNA adduct formation and acrosome status of sperm cells. Semen quality parameters were analyzed according to 2010 World Health Organization criteria in 122 non-smokers and 125 cigarette smokers attending tertiary health care centre in Barak Valley of Assam, India followed by BPDE-DNA adduct formation and acrosome status test. A significant decline was found in sperm concentration, progressive motility, morphology in smokers (p
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Abstract The purpose of the present study was to evaluate the effects of aflatoxin B1 (AFB1) and benzo[a]pyrene (BaP) on the heart muscle of chicken embryos of the broiler line Ross 308. The benzo[a]pyrene in the organic oil solution was... more
Abstract The purpose of the present study was to evaluate the effects of aflatoxin B1 (AFB1) and benzo[a]pyrene (BaP) on the heart muscle of chicken embryos of the broiler line Ross 308. The benzo[a]pyrene in the organic oil solution was injected in ovo on the 6th day of the incubation in doses of: 0.1, 0.5, and 1 mg/kg weight of eggs; the aflatoxin B1 in the organic oil solution was injected in ovo on the 6th day of the incubation into the yolk in doses of 80, 120 and 240 ng/kg weight of eggs. Multiple biochemical and hepatic parameters have been observed, including sodium, potassium, chloride, cholesterol, uric acid, total proteins, aminotransferase aspartate, and aminotransferase alanine. A low dose of AFB1 and BaP administered in ovo during early embryonic development had a significant impact on chicken embryonic development, as demonstrated by alterations in biochemical, mineral, and hepatic parameters.
Research Interests: Chemistry, Medicine, Multidisciplinary, Incubation, Aflatoxin, and 4 moreUric Acid, Pyrene, Broiler, and Yolk
Acrylamide monomer causes neurotoxicity, male reproductive toxicity and endocrine – related tumors in rodents. Acrylamide was found in food, heat-processed potato products, coffee, bread and has been classified as probable human... more
Acrylamide monomer causes neurotoxicity, male reproductive toxicity and endocrine – related tumors in rodents. Acrylamide was found in food, heat-processed potato products, coffee, bread and has been classified as probable human carcinogen by IARC. Daily intake from foodstuff was estimated in the range of 0.3-0.8 mg/kg bw per day (Seal et al., 2008). Glutathione peroxidase catalyzes the reduction hydrogen peroxide by glutathione. Extracellular superoxide dismutase catalyzes the dismutation of superoxide radical to hydrogen peroxide and oxygen. The activity of superoxide dismutase and glutathione peroxidase has been examined. The research was conducted on SWISS mice 8 week old, weight 26 g. Decapitation was carried out after 48, 72 and 192 hours after acrylamide injection. Animals received two doses of acrylamide 20 mg/kg and 40 mg/kg with intraperitoneal injection three times a week. Control group received physiological saline 3 times a week. The activity of superoxide dismutase was...
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In this study distribution of nickel as a risk factor of environment in testis and its effects on the testicular structure in experimental animals and effect on spermatozoa was analyzed. In this study the effect of Ni on the testicular... more
In this study distribution of nickel as a risk factor of environment in testis and its effects on the testicular structure in experimental animals and effect on spermatozoa was analyzed. In this study the effect of Ni on the testicular structure after an experimental intraperitoneal (i.p.) administration, concentration of nickel in semen of different animal species and the effect of in vitro spermatozoa incubation with nickel on the spermatozoa motility and membrane changes is reported. Our findings clearly suggest a negative effect of nickel on the structure as well as on the function of seminiferous epithelium. In experimental groups with nickel a significant (p 250 μM Ni/ml) cause decrease of spermatozoa motility in vitro.
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In the present study the effect of collection regime on rabbit semen development was analyzed. Eight seminal traits, such as overall ejaculate volume (VOL), spermatozoa concentration (CON), motility (MOT), progressive motility (PRO),... more
In the present study the effect of collection regime on rabbit semen development was analyzed. Eight seminal traits, such as overall ejaculate volume (VOL), spermatozoa concentration (CON), motility (MOT), progressive motility (PRO), percentage of progressive motility (% PRO), curvilinear velocity (VCL), beat-cross frequency (BCF) and amplitude of lateral head displacement (ALH) were analyzed. As the number of collections increased, more bucks were able to ejaculate. During collections the semen volume gradually increased from 0.4 ml to 1.0 ml with the average of 0.68 ± 0.34 ml. The average of MOT was 55.55 ± 26.63 % and the average of PRO reached 40.43 ± 27.35 %. The other assessed parameters were CON - 0.547 ± 0.165 x 10 9 ml -1 ; ALH - 4.18 ± 1.18 µm; BCF - 27.42 ± 7.12 Hz; VCL - 104.04 ± 37.00 µm.s -1 �� A positive correlation between ejaculate volume and the number of collections was found. MOT and PRO significantly correlated with the number of collections. In addition, positi...
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concentrations of cooper, zinc, iron, manganese, cadmium, lead and nickel in the semen of rabbits (n=27) in relation to spermato zoa quality were evaluated. samples were analyzed on an atomic absorption spectrophotometer (Unicam solar 939).
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The target of this study was to evaluate the effect of extract of the European mistletoe – Viscum album quercus L. on spermatozoa motility and viability in vitro. The CASA system was used to determine the spermatozoa motility parameters... more
The target of this study was to evaluate the effect of extract of the European mistletoe – Viscum album quercus L. on spermatozoa motility and viability in vitro. The CASA system was used to determine the spermatozoa motility parameters at different time intervals (0, 1, 2 and 3 h) and spermatozoa viability was determined in five different doses of Viscum album quercus L [10 (QA), 6.6 (QB), 3.3 (QC), 2.5 (QD) and 2 (QE) mg/ml]. Results in experimental groups detected a significant deterioration on rabbit spermatozoa after 1, 2 and 3 hours, compared to the control. The initial total spermatozoa motility showed increased value for all doses of Viscum album quercus in comparison to control. After in vitro culture a dose–dependent decrease (QA: reduction of 69.7 %, QB: reduction of 40.9 %) was found. For the progressive spermatozoa most significant decrease (86.8 % for QA vs. 48.5 % for QB) was detected compared to the control after 3 hours of culture. Spermatozoa viability (MTT test) w...
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Nickel is a ubiquitous environmental pollutant, which has various effects on reproductive endocrinology. In this study, human adrenocortical carcinoma (NCI-H295R) cell line was used as an in vitro biological model to study the effect of... more
Nickel is a ubiquitous environmental pollutant, which has various effects on reproductive endocrinology. In this study, human adrenocortical carcinoma (NCI-H295R) cell line was used as an in vitro biological model to study the effect of nickel chloride (NiCl2) on the viability and steroidogenesis. The cells were exposed to different concentrations (3.90; 7.80; 15.60; 31.20; 62.50; 125; 250 and 500 μM) of NiCl2 and compared with control group (culture medium without NiCl2). The cell viability was measured by the metabolic activity assay. Production of sexual steroid hormones was quantified by enzyme linked immunosorbent assay. Following 48 h culture of the cells in the presence of NiCl2 a dose-dependent depletion of progesterone release was observed even at the lower concentrations. In fact, lower levels of progesterone were detected in groups with higher doses (≥125 μM) of NiCl2 (P<0.01), which also elicited cytotoxic action. A more prominent decrease in testosterone production (...
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The aim of this study was to evaluate the effect of induced training on the horses’ metabolism during an experiment lasting nine weeks where we continually scaled up the load on the horses by three defined stages. Blood was obtained from... more
The aim of this study was to evaluate the effect of induced training on the horses’ metabolism during an experiment lasting nine weeks where we continually scaled up the load on the horses by three defined stages. Blood was obtained from eighteen horses – two stallions, eight mares and eight geldings. In the experiment, we focused on the biochemical analysis of the blood plasma on multiple mineral profile indicators – Ca, P, Mg, K, Cl and Na, and some other variables (energy, nitrogen, AST, ALT, glucose, urea, creatinine kinase, total proteins). The result showed significant changes between the groups in most indicators. A significant increase in the potassium, phosphorus and calcium and a decrease in the concentrations of magnesium over the course of the experiment were found. For the other indicators, a significant increase in the activities of the AST and ALT out of the other indicators and the fluctuating values in the total proteins were noticed. Summarised, significant changes...
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The goal of this study was to observe the effect of resorcinol on motility, viability and morphology of bovine spermatozoa. The semen was used from six randomly chosen breeding bulls. Ejaculate was diluted by different solutions of... more
The goal of this study was to observe the effect of resorcinol on motility, viability and morphology of bovine spermatozoa. The semen was used from six randomly chosen breeding bulls. Ejaculate was diluted by different solutions of resorcinol in 1:40 ratio. Samples were divided into 7 groups with different concentrations of resorcinol (Control, RES1 – 4 mg/ml, RES2 – 2 mg/ml, RES3 – 1 mg/ml, RES4 – 0.5 mg/ml, RES5 – 0.25 mg/ml and RES6 – 0.125 mg/ml). Motility of spermatozoa was detected using CASA method at temperature of 37 °C in time periods 0, 1, 2, 3, 4 hours from the start of the experiment. Significant motility differences between all groups except control and RES6 with difference of 5.58 %, as well as between RES1 and RES2 groups with difference of 2.17 % were found. Progressive motility had the same significant differences. Spermatozoa viability (MTT test) decreased compared to control in all experimental groups during the entire duration of experiment. Observing morphologi...
Research Interests: Chemistry, Andrology, Medicine, Motility, Physiological, and 4 moreMedical Physiology, Semen, Resorcinol, and flagellum
The purpose of present study was to observe the effect of resorcinol on bovine spermatozoa before and after cryopreservation. Fresh semen was obtained from six randomly chosen breeding bulls. Experimental samples were prepared by diluting... more
The purpose of present study was to observe the effect of resorcinol on bovine spermatozoa before and after cryopreservation. Fresh semen was obtained from six randomly chosen breeding bulls. Experimental samples were prepared by diluting the semen with six different concentrations of resorcinol (REZ1– 4; REZ2 – 2; REZ3 – 1; REZ4 – 0.5; REZ5 – 0.25 and REZ6 –0.152 mg.ml-1). Experimental groups were compared against control group (REZK). Using CASA method spermatozoa motility was evaluated before cryopreservation and also after thawing, during incubation at 37°C in time periods 0, 5, 10, 15 and 60 minutes. Negative effect of in vitro resorcinol addition was observed in all analysed parameters (MOT, PRO, VCL, ALH and BCF) in groups REZ1, REZ2 and REZ3. In contrast, positive impact mainly on motility and progressive motility was found in experimental groups with the lowest resorcinol concentration addition (REZ4, REZ5 and REZ6) in comparison to the control group. After thawing signific...
Research Interests: Chemistry, Andrology, Cryopreservation, Motility, In Vitro, and 3 moreIncubation, Semen, and Resorcinol
The goal of this study is to summarize the current knowledge on the effects of one of the essential metals, copper (Cu) on the reproductive system. The development of past four decades addressing effects of Cu on reproductive organs is... more
The goal of this study is to summarize the current knowledge on the effects of one of the essential metals, copper (Cu) on the reproductive system. The development of past four decades addressing effects of Cu on reproductive organs is reviewed. The most relevant data obtained from in vivo and in vitro experiments performed on humans and other mammals, including effects of copper nanoparticles (CuNPs) on the reproductive functions are presented. Short term Cu administration has been found to exert deleterious effect on intracellular organelles of rat ovarian cells in vivo. In vitro administration in porcine ovarian granulosa cells releases insulin-like growth factor (IGF-I), steroid hormone progesterone (P4), and induces expression of peptides related to proliferation and apoptosis. Adverse effect of Cu on male reproductive functions has been indicated by the decrease in spermatozoa parameters such as concentration, viability and motility. Copper nanoparticles are capable of generat...
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The purpose of this study was to evaluate the impact of caffeine on turkey spermatozoa during in vitro incubation. Experimental samples were prepared by diluting the raw semen with nine different concentrations of caffeine – from 0.078125... more
The purpose of this study was to evaluate the impact of caffeine on turkey spermatozoa during in vitro incubation. Experimental samples were prepared by diluting the raw semen with nine different concentrations of caffeine – from 0.078125 mg/mL to 10 mg/mL. The individual motility parameters were evaluated by the Computer Assisted Semen Analyser (CASA) system, and the viability of spermatozoa was evaluated using eosin-nigrosin staining. Selected parameters were recorded at six time periods: 0, 1, 2, 3, 4 and 5 h at 5 °C and 41 °C. A significantly higher motility and progressive motility of spermatozoa (P < 0.01 and P < 0.001, respectively) was detected in the samples containing caffeine ranging from 0.15625 to 7.5 mg/mL as compared to the control sample at 5 °C. At an incubation temperature of 41 °C the positive effect of caffeine on motility parameters was observed only at the beginning of incubation (at times 0 and 1). The tested caffeine concentrations showed no significant...
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DOAJ Directory of Open Access Journals, SPARC Europe Award 2009 English. Free, full text, quality controlled scientific and scholarly journals, covering all subjects and many languages. ...
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Background: Conservation of species diversity is the need of the hour for preserving life forms on Earth. Extinction of any part of the ecosystem has negative impacts on many processes and systems. The objective of this work was to... more
Background: Conservation of species diversity is the need of the hour for preserving life forms on Earth. Extinction of any part of the ecosystem has negative impacts on many processes and systems. The objective of this work was to analyze some biochemical and molecular indicators and their correlations to biogenic elements and heavy metals in Testudo hermanni (n = 16). Methods: Biochemical parameters were analyzed using the commercial kit DiaSys and biochemical analyzer Randox RX Monza. Sodium, potassium, and chlorides were measured using the EasyLite analyzer. Oxidative stress was evaluated using colorimetric and luminometric methods. Quantification of chemical elements in the blood was carried out using inductively coupled plasma mass spectrometry (ICPS). Results: Biochemical values of analyzed samples from Hermann’s tortoises were almost the same as referential values described by multiple authors, with minor aberrations in the total protein parameter. Values of arsenic (As) and...
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This study monitored the chemical and biochemical composition of bovine seminal plasma (SP). Freshly ejaculated semen (n = 20) was aliquoted into two parts. The first aliquot was immediately assessed to determine the sperm motion... more
This study monitored the chemical and biochemical composition of bovine seminal plasma (SP). Freshly ejaculated semen (n = 20) was aliquoted into two parts. The first aliquot was immediately assessed to determine the sperm motion parameters. Another motility measurement was performed following an hour-long co-incubation of spermatozoa with SP at 6 °C. The other aliquot was processed to obtain the SP. Seminal plasma underwent the analyses of chemical composition and quantification of selected proteins, lipids and RedOx markers. Determined concentrations of observed parameters served as input data to correlation analyses where associations between micro and macro elements and RedOx markers were observed. Significant correlations of total oxidant status were found with the content of Cu and Mg. Further significant correlations of glutathione peroxidase were detected in relation to Fe and Hg. Furthermore, associations of chemical elements and RedOx markers and spermatozoa quality parame...
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The aim of this study was to describe the effects of nickel (NiCl2) on murine testicular structure. Experimental animals were injected intraperitoneally with a single dose of 20 mg NiCl2 per kg of body mass (group A, n = 5) and 40 mg... more
The aim of this study was to describe the effects of nickel (NiCl2) on murine testicular structure. Experimental animals were injected intraperitoneally with a single dose of 20 mg NiCl2 per kg of body mass (group A, n = 5) and 40 mg NiCl2 per kg b. m. (group B, n = 5). The group without injection (n = 5) was the control (C). Animals were killed 48 hours after administration of nickel. The body mass of animals, the mass of testes and the testes: body mass ratio were not significantly affected. In both experimental groups a significant (p < 0.001) decrease of germinal epithelium in comparison with control group was observed. The relative volume of the interstitium was increased but not significantly in both experimental groups. An increase in the relative volume of the lumen was registered in both experimental groups in comparison with the control group. The qualitative analysis detected a dilatation of blood vessels in the interstitium, undulation of the basal membrane and severa...
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Research Interests: Medicine and Correlation
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Target of this study was to analyze the effect of different diluents (saline solution, commercial diluent and yolk diluent) on the functional parameters of turkey spermatozoa after a culture in vitro at 5 °C and 20 °C. Samples were... more
Target of this study was to analyze the effect of different diluents (saline solution, commercial diluent and yolk diluent) on the functional parameters of turkey spermatozoa after a culture in vitro at 5 °C and 20 °C. Samples were subjected to repeated measurements at intervals of 0, 30, 60, 90, 120, 180, 240, 300 minutes. Significantly the highest motility was found in the samples with yolk diluent at 5 °C and at 20 °C. Lower values were measured in samples diluted with saline solution and commercial diluent. Progressive motility copied the finding results of the overall motility with the exception of yolk diluent, which were reported significantly lower values.
The aim of our study was to determine the effect of single nickel administration as well as co–administration with zinc on distribution of potassium and magnesium in the muscle tissue (musculus longissimus dorsi, musculus biceps femoris)... more
The aim of our study was to determine the effect of single nickel administration as well as co–administration with zinc on distribution of potassium and magnesium in the muscle tissue (musculus longissimus dorsi, musculus biceps femoris) of rabbits. In the experiment 25 female rabbits of broiler line Californian were involved. Animals were divided to three groups: K (n=5) – control; P1 (n=5) – received 17.5 g NiCl2.100 kg of feed mixture, P2 (n=5) – received 35 g NiCl2.100 kg of feed mixture (FM), P3 (n = 5) – received 17.5 g NiCl2.100 kg and 30 g ZnCl2.100 kg of feed mixture and P4 (n = 5) – received 35 g NiCl2.100 kg and 30 g ZnCl2.100 kg of feed mixture. Animals were fed ad libitum using KKV1 feeding mixture with or without nickel and zinc addition for 90 days. For analysis of the content of these trace elements in 50 samples of animals an atomic absorption spectrophotometry method was used. The significant higher value (p<0.05) of potassium in musculus longissimus dorsi in gr...
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In recent years, many studies have been focused on natural substances that can affect health of animals. A mix of different extracts was used as dietary supplement and it consists exclusively of natural products. Its main components are... more
In recent years, many studies have been focused on natural substances that can affect health of animals. A mix of different extracts was used as dietary supplement and it consists exclusively of natural products. Its main components are polyphenols from terrestrial and marine origins and plant polysaccharides. The effect of this supplement on reproduction has not been reviewed in the past what is a reason why its effect on the reproduction potential of male rabbits was tested. The aim of the present study is to determine effects of natural mix during 120-days long in vivo experiment on selected reproductive traits of male rabbits. Natural mix was supplemented in two different concentrations (T1 – 0.3% and T2 – 0.6%) with the basal ingredients of the conventional rabbit feed in pellet form. In our experiments, emphasis was placed on both the spermatozoa concentration and its motility parameters as well as on the properties of seminal plasma and antioxidant activity. The dietary suppl...
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The aim of this review is to refer a possibility of using natural substances for treating or reducing the symptoms of tinnitus. Tinnitus is a sensation of sound without an external source. It often manifests as a ringing in the ears, but... more
The aim of this review is to refer a possibility of using natural substances for treating or reducing the symptoms of tinnitus. Tinnitus is a sensation of sound without an external source. It often manifests as a ringing in the ears, but it may also sound like a buzzing, hissing, whistling or even roaring in the head. Tinnitus is a symptom of an underlying condition. It can be linked to hearing loss, stress, ear damage, blood pressure, tumours and atherosclerosis. Exposure to loud noise is one major cause of tinnitus, since it wears down the delicate hair cells in inner ear that translate sounds into nerve impulses. Potential therapy of tinnitus is a pharmacological treatments. Fortunately, there are many effective natural alternatives to drugs that can bring considerable relief and help cope. The potential form of treatment is vitamins and natural flavonoids therapy. Low levels of melatonin and vitamin B12 in body have a significant correlation with the development of tinnitus. It ...
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In this study the NCI-H295R human adrenocortical carcinoma cell line was used as an in vitro biological model to study the effect of mercury (HgCl(2)) on the steroidogenesis. The cells were cultured for 48 h with addition of 1.0; 5.0; 25;... more
In this study the NCI-H295R human adrenocortical carcinoma cell line was used as an in vitro biological model to study the effect of mercury (HgCl(2)) on the steroidogenesis. The cells were cultured for 48 h with addition of 1.0; 5.0; 25; 50 or 100 μM of HgCl(2) and compared to control. Cell viability was measured by the MTT (metabolic activity) assay estimation for the mitochondria structural integrity. Quantification of testosterone and progesterone directly from aliquots of the medium was performed by enzyme linked immunosorbent assay (ELISA). Concentration-dependent depression in testosterone production was detected particularly for higher concentration of Hg(2+). Progesterone production was also decreased, but at the lower concentrations (1.0 and 5.0 μM) of Hg(2+) this decline was lower compared to depression of testosterone. The cell viability significantly decreased at 25 μM and higher concentration of Hg(2+). However, at 25 μM Hg(2+) exposure the cell viability remained relatively high (&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 80%). Results of the study indicate dose-dependent decreases in both testosterone and progesterone production of H295R cell culture following a 48 h in vitro HgCl(2) exposure. The results suggest that Hg has detrimental effects on steroid hormone synthesis also at very low concentrations and consecutively on reproductive physiology.
Research Interests: Endocrinology, Chemistry, Biology, Medicine, Multidisciplinary, and 15 moreCell Culture, In Vitro, Progesterone, Humans, Internal Medicine, Cell Death, Cell Viability, Hormone, Environmental Pollutants, Cell Survival, Enzyme Linked Immunosorbent Assay, Adrenocortical Carcinoma, Gonads, Mercuric Chloride, and Adrenal glands
Research Interests: Endocrinology, Biology, Transmission Electron Microscopy, Quantitative analysis, Endoplasmic Reticulum, and 11 moreUltrastructure, Cadmium, Qualitative Analysis, Fine Structure Constant, Endothelial cell, Stromal Cells, Body Weight, Quantitative Analysis, Veterinary Sciences, Control Group, and Pharmacology and pharmaceutical sciences
Cadmium (Cd) is an environmental toxicant, capable of reducing mitochondrial ATP production and promoting the formation of reactive oxygen species (ROS) with resultant oxidative stress conditions. The ovary and testis are the primary... more
Cadmium (Cd) is an environmental toxicant, capable of reducing mitochondrial ATP production and promoting the formation of reactive oxygen species (ROS) with resultant oxidative stress conditions. The ovary and testis are the primary gonads in which female gametes (oocytes) and male gametes (spermatozoa), estrogen and testosterone are produced. These organs are particularly susceptible to Cd cytotoxicity due to their high metabolic activities and high energy demands. In this review, epidemiological and experimental studies examining Cd toxicities in gonads are highlighted together with studies using zinc (Zn), selenium (Se), and natural substances to reduce the effects of Cd on follicular genesis and spermatogenesis. Higher blood concentrations of Cd ([Cd]b) were associated with longer time-to-pregnancy in a prospective cohort study. Cd excretion rate (ECd) as low as 0.8 μg/g creatinine was associated with reduced spermatozoa vitality, while Zn and Se may protect against spermatozoa...