A fully validated, sensitive and specific method for the extraction and quantification of Δ 9 -tetrahydrocannabinol (THC) and 11-nor-9-carboxy-Δ 9 -THC (THC-COOH) and for the detection of 11-hydroxy-Δ 9 -THC (11-OH THC) in oral fluid,... more
A fully validated, sensitive and specific method for the extraction and quantification of Δ 9 -tetrahydrocannabinol (THC) and 11-nor-9-carboxy-Δ 9 -THC (THC-COOH) and for the detection of 11-hydroxy-Δ 9 -THC (11-OH THC) in oral fluid, urine and whole blood is presented. Solid-phase ...
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Amitriptyline is a tricyclic antidepressant widely used in the treatment of depression. Antidepressant drugs are among the most commonly encountered causes of self-poisoning, as illustrated by several published cases in the literature.... more
Amitriptyline is a tricyclic antidepressant widely used in the treatment of depression. Antidepressant drugs are among the most commonly encountered causes of self-poisoning, as illustrated by several published cases in the literature. This investigation reports a case of massive amitriptyline intoxication, involving a 44-year old female found dead in bed. The presence of this tricyclic antidepressant was revealed by a routine screening procedure. The concentration was calculated by gas chromatography/ electron ionization-mass spectrometry in the selected ion monitoring mode after solid-phase extraction using proadifen as internal standard and was in the post-mortem whole blood sample 85.9 μg/mL. This value was much higher than the reported toxic values ever found in the literature, and may therefore have caused the victim's death. Nortriptyline was also detected in the toxic concentration range, as well as therapeutic levels of diazepam and nor-diazepam. Taking into account both the available circumstantial information and toxicological results, it is very likely that death was caused by self-poisoning. Human & Experimental Toxicology (2007) 26, 667—670
Research Interests: Depression, Mass Spectrometry, Suicide, Medicine, Forensic Toxicology, and 15 moreHuman, Humans, Antidepressant, Solid Phase Extraction, Female, Gas Chromatography, Human Experimentation, Liquid Chromatography / Electrospray Ionization Mass Spectrometry, Adult, Gas Chromatography/mass Spectrometry, Diazepam, Amitriptyline, Whole-blood, Nortriptyline, and Pharmacology and pharmaceutical sciences
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Research Interests: Chemistry and Disulfiram
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Amitriptyline is a tricyclic antidepressant widely used in the treatment of depression. Antidepressant drugs are among the most commonly encountered causes of self-poisoning, as illustrated by several published cases in the literature.... more
Amitriptyline is a tricyclic antidepressant widely used in the treatment of depression. Antidepressant drugs are among the most commonly encountered causes of self-poisoning, as illustrated by several published cases in the literature. This investigation reports a case of massive amitriptyline intoxication, involving a 44-year old female found dead in bed. The presence of this tricyclic antidepressant was revealed by a routine screening procedure. The concentration was calculated by gas chromatography/ electron ionization-mass spectrometry in the selected ion monitoring mode after solid-phase extraction using proadifen as internal standard and was in the post-mortem whole blood sample 85.9 μg/mL. This value was much higher than the reported toxic values ever found in the literature, and may therefore have caused the victim's death. Nortriptyline was also detected in the toxic concentration range, as well as therapeutic levels of diazepam and nor-diazepam. Taking into account bot...
Research Interests: Depression, Mass Spectrometry, Suicide, Medicine, Forensic Toxicology, and 13 moreHuman, Humans, Solid Phase Extraction, Female, Gas Chromatography, Human Experimentation, Liquid Chromatography / Electrospray Ionization Mass Spectrometry, Adult, Gas Chromatography/mass Spectrometry, Diazepam, Amitriptyline, Nortriptyline, and Pharmacology and pharmaceutical sciences
A new and sensitive method to determine ticlopidine in whole human blood using proadifen as the internal standard (IS) is described. The analyte and IS were extracted by solid-phase extraction using Oasis HLB cartridges, and the extracts... more
A new and sensitive method to determine ticlopidine in whole human blood using proadifen as the internal standard (IS) is described. The analyte and IS were extracted by solid-phase extraction using Oasis HLB cartridges, and the extracts were analyzed by gas chromatography-electron impact ionisation-mass spectrometry (GC/EI-MS). Calibration curves were established daily in spiked blood samples using a non-linear calibration model, between 0.01 and 4.5 microg/mL. The correlation coefficients were higher than 0.995. Precision and accuracy fulfilled the internationally accepted criteria (coefficients of variation were less than 9%, and the measured concentrations were within +/-7% of the true value). Limits of detection and quantitation were respectively, 3 and 10 ng/mL. No interfering substances were detected by analysis of 10 blank blood samples of different origin. Mean recovery, calculated at three concentration levels, was 71%. Because of its simplicity and speed, the proposed method can be applied in the determination of this inhibitor of platelet aggregation in whole blood samples, and is suitable for application in toxicology routine analysis.