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    Nicholas Paretti

    We exposed a native endangered species, bonytail chub (Gila elegans), to graded concentrations of secondarily-treated effluent. At the end of each treatment period, we collected water from all raceways and samples were analyzed for the... more
    We exposed a native endangered species, bonytail chub (Gila elegans), to graded concentrations of secondarily-treated effluent. At the end of each treatment period, we collected water from all raceways and samples were analyzed for the presence of 83 organic wastewater compounds. We extracted blood plasma from control and treatment fish in each raceway and samples were analyzed for hormones, 17β-estradiol and 11-ketotestosterone, and the egg yolk protein, vitellogenin.17β-estradiol concentrations were consistently lower in treatment females than those detected in control females. The 17β-estradiol and vitellogenin concentrations were always higher in treatment males than those found in control males. Concentrations of 11-ketotestosterone were consistently lower in treatment males than those detected in control males.Endocrine disrupting effects occurred in male and female fish exposed to low concentrations of effluent. Changes in hormone levels suggested a feminizing effect in tr...
    In the borderland region of the desert southwest, human health and the ecosystems upon which humans rely largely depend on the quality, quantity, and distribution of water resources. In the Santa Cruz River Watershed (SCW), located in the... more
    In the borderland region of the desert southwest, human health and the ecosystems upon which humans rely largely depend on the quality, quantity, and distribution of water resources. In the Santa Cruz River Watershed (SCW), located in the Arizona and Sonora, Mexico border region, surface water is scarce and unreliable, and, during much of the year, is composed of effluent from the local wastewater treatment plant. This makes groundwater the preferred and, consequently, primary source for industrial, agricultural, and domestic use. USGS scientists are using an integrative approach, incorporating the expertise of the Geography, Water, Biology, and Geology disciplines to identify risks to water resources in the SCW, and the potential for impacts to riparian ecosystems and ultimately, human health. This includes tracking organic and inorganic contaminants and their effects from sources to sinks in sediment, water, plants, and animals. Existing ground- and surface-water models will be us...
    Research Interests:
    A continuous active sampling method was compared to continuous passive and discrete sampling methods for the sampling of trace organic compounds (TOCs) in water. Results from each method are compared and contrasted in order to provide... more
    A continuous active sampling method was compared to continuous passive and discrete sampling methods for the sampling of trace organic compounds (TOCs) in water. Results from each method are compared and contrasted in order to provide information for future investigators to use while selecting appropriate sampling methods for their research. The continuous low-level aquatic monitoring (CLAM) sampler (C.I.Agent® Storm-Water Solutions) is a submersible, low flow-rate sampler, that continuously draws water through solid-phase extraction media. CLAM samplers were deployed at two wastewater-dominated stream field sites in conjunction with the deployment of polar organic chemical integrative samplers (POCIS) and the collection of discrete (grab) water samples. All samples were analyzed for a suite of 69 TOCs. The CLAM and POCIS samples represent time-integrated samples that accumulate the TOCs present in the water over the deployment period (19-23 h for CLAM and 29 days for POCIS); the di...