In plants, ureases have been related to urea degradation, to defense against pathogenic fungi and phytophagous insects, and to the soybean-Bradyrhizobium japonicum symbiosis. Two urease isoforms have been described for soybean: the... more
In plants, ureases have been related to urea degradation, to defense against pathogenic fungi and phytophagous insects, and to the soybean-Bradyrhizobium japonicum symbiosis. Two urease isoforms have been described for soybean: the embryo-specific, encoded by Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding locus exists in the completed soybean genome. The gene was designated Eu5 and the putative product of its ORF as SBU-III. Phylogenetic analysis shows that 41 plant, moss and algal ureases have diverged from a common ancestor protein, but ureases from monocots, eudicots and ancient species have evolved independently. Genomes of ancient organisms present a single urease-encoding gene and urease-encoding gene duplication has occurred independently along the evolution of some eudicot species. SBU-III has a shorter amino acid sequence, since many gaps are found when compared to other sequences. A mutation in a highly conserved amino acid residue suggests absence of ureolytic activity, but the overall protein architecture remains very similar to the other ureases. The expression profile of urease-encoding genes in different organs and developmental stages was determined by RT-qPCR. Eu5 transcripts were detected in seeds one day after dormancy break, roots of young plants and embryos of developing seeds. Eu1 and Eu4 transcripts were found in all analyzed organs, but Eu4 expression was more prominent in seeds one day after dormancy break whereas Eu1 predominated in developing seeds. The evidence suggests that SBU-III may not be involved in nitrogen availability to plants, but it could be involved in other biological role(s).
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ABSTRACT Bromeliaceae is a Neotropical family that evolved ecological key innovations in association with extensive adaptive radiation. Its species present a variety of different mating system strategies varying within and among species,... more
ABSTRACT Bromeliaceae is a Neotropical family that evolved ecological key innovations in association with extensive adaptive radiation. Its species present a variety of different mating system strategies varying within and among species, within genera and subfamilies. Also, species with a wide geographical range can display large variation in mating system, reproductive success and genetic diversity. Here we combined data from hand pollinations and genetic analysis to assess outcomes of contemporary gene flow and mating system variation at the range edge of Vriesea gigantea. Results from pollen germination rates showed that this species is cryptically self-incompatible. Hand-pollination experiments and genetic analysis of progeny arrays revealed that V. gigantea has a mixed mating system, with high selfing rates (s = 0.612), and high inbreeding coefficient (F = 0.372). Inbreeding in V. gigantea at southern edge of its distribution range was caused by high levels of selfing rather than by mating among relatives. Moreover, strong pollen pool genetic structure was observed (Φ'FT = 0.671), with an increase from north to south. The parameters observed help us to understand historical and ecological conditions under which V. gigantea has experienced moderate to high levels of selfing in the face of reduced pollen flow from central to peripheral populations due to recent southward range expansion.
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Research Interests: Microbiology, Regeneration, Plant Biology, Transcription Factors, Disease susceptibility, and 13 moreGene Silencing, Plant Genome Project, Plant diseases, Sequence alignment, Genetic transformation, Soybeans, Host Pathogen Interactions, Amino Acid Sequence, PLANT PROTEINS, BASIDIOMYCOTA, Gene expression profiling, Plant Leaves, and Molecular Sequence Data
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... Adriana Giongo I ; Anelise Beneduzi I ; Adriana Ambrosini I ; Luciano Kayser Vargas II ; MarcosRoberto Stroschein III ; Flávio Luiz Eltz III ... MOSCHETTI, G.; PELUSO, AL; PROTOPAPA, A.; ANASTASIO, M.; PEPE, O. & DEFEZ, R. Use... more
... Adriana Giongo I ; Anelise Beneduzi I ; Adriana Ambrosini I ; Luciano Kayser Vargas II ; MarcosRoberto Stroschein III ; Flávio Luiz Eltz III ... MOSCHETTI, G.; PELUSO, AL; PROTOPAPA, A.; ANASTASIO, M.; PEPE, O. & DEFEZ, R. Use of nodulation pattern, stress tolerance, nodC ...
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Research Interests: Genetics, Microbiology, Biomass, Medical Microbiology, Brazil, and 32 moreBiodiversity, Genetic Diversity, Phylogeny, Nitrogen Fixation, Microbiology and Biotechnology, Gram Positive, Siderophores, Oxidoreductases, Bacteria, Plant Growth Regulators, Profitability, Plant growth, Rio Grande do Sul, Nitrogen, Iron, Indole acetic acid (IAA) production, Plant Roots, Phenotype, Soil Microbiology, Enzyme, Plant Growth Promoting Rhizobacteria, Genotype, Gramineae, Environmental Stress, Dry Matter, Triticum, Southern Brazil, Bacillus Sp, Cropping System, Low molecular weight, Indole Acetic Acid, and Molecular Sequence Data
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... I ; Anelise Machado da Silva III ; Iliane Freitas de Souza II ; Beatriz Wiebke-Strohm III ; Lauro Bücker-Neto III ; Marta Bencke III ... SATO, S.; NEWELL, C.; KOLACZ, K.; TREDO, L.; FINER, J.; HINCHEE, M. Stable transformation via... more
... I ; Anelise Machado da Silva III ; Iliane Freitas de Souza II ; Beatriz Wiebke-Strohm III ; Lauro Bücker-Neto III ; Marta Bencke III ... SATO, S.; NEWELL, C.; KOLACZ, K.; TREDO, L.; FINER, J.; HINCHEE, M. Stable transformation via particle bombardment in two different soybean ...
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Research Interests: Evolutionary Biology, Genetics, Climate Change, Genomics, Population Genetics, and 20 moreBrazil, Heredity, Genetic Diversity, Cytogenetics, Phylogeny, Pollen, Genetic Structure, Rio de Janeiro, Ecological, Species Diversity, Oscillations, Trees, Atlantic Rainforest, Cell nucleus, Angiosperms, Ecosystem, Population Genetic Structure, Genetic variation, Tropical Climate, and DNA marker
The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease... more
The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was eva...
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In this work, we further analyzed an Azospirillum brasilense Sp7 mutant (Sp7::Tn5-33) showing a pleiotrophic phenotype due to a Tn5 insertion into an open reading frame of 840 bp (orf280). The deduced amino acid sequence of this region... more
In this work, we further analyzed an Azospirillum brasilense Sp7 mutant (Sp7::Tn5-33) showing a pleiotrophic phenotype due to a Tn5 insertion into an open reading frame of 840 bp (orf280). The deduced amino acid sequence of this region has high similarity to a family of universal stress proteins. Because the most interesting property exhibited by the Sp7::Tn5-33 mutant was an enhanced in vitro nitrogen fixation activity, we addressed the question of whether it could benefit the host plant. We found that the increased nitrogenase activity at the free-living state of the mutant bacterium was correlated with an increased production of the nitrogenase reductase protein (NifH), in amounts approximately 1.5 times higher than the wild type. The mutant strain exhibited the same level of auxin production and the same colonization pattern of wheat roots as the wild type. We also observed that Sp7::Tn5-33 increased the total plant dry weight, although the N content did not differ significantly between wheat plants inoculated with mutant or wild-type strains.
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Abstract The pine processionary moth Thaumetopoea pityocampa Den. and Schiff.(Lep., Thaumetopoeidae) is one of the most harmful insect pests for pine species in Mediterranean countries including Turkey. Two Bacillus thuringiensis isolates... more
Abstract The pine processionary moth Thaumetopoea pityocampa Den. and Schiff.(Lep., Thaumetopoeidae) is one of the most harmful insect pests for pine species in Mediterranean countries including Turkey. Two Bacillus thuringiensis isolates obtained from T. pityocampa were identified and characterized in terms of crystal shape using electron microscopy, SDS–PAGE analysis, cry gene contents, H-serotype and insecticidal activity. Examination by a scanning electron microscope showed that Tp6 and Tp14 isolates have flat square and ...
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Research Interests: Soil Science, Ecology, Genetic Diversity, Biological Sciences, Environmental Sciences, and 16 moreBacteria, Profitability, Rio Grande do Sul, Soybean, Bacterial Diversity, Genotype, Environmental Conditions, AFLP, soil pH, Indexation, Glycine max, Diversity index, Organic Matter, Cropping System, Statistical Approach, and Microbial community
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Somatic embryogenesis was achieved from cell suspension cultures of niger (Guizotia abyssinica Cass.). Initially, friable embryogenic calluses were induced from cotyledonary leaves of niger on Murashige and Skoog (MS) agar medium... more
Somatic embryogenesis was achieved from cell suspension cultures of niger (Guizotia abyssinica Cass.). Initially, friable embryogenic calluses were induced from cotyledonary leaves of niger on Murashige and Skoog (MS) agar medium containing 5 μM 2,4-Dichlorophenoxyacetic acid (2,4-D) and 0.5 μM kinetin (KIN). Cell suspension cultures were established by using embryogenic calluses in MS liquid medium containing 5 μM 2,4-D and 0.5 μM KIN. Initiation
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Plant fertility is a central subject of many questions in plant evolutionary and conservation biology. Pollen availability, abiotic resources, and flowering pattern can limit fruit and seed production. Open pollination and pollen... more
Plant fertility is a central subject of many questions in plant evolutionary and conservation biology. Pollen availability, abiotic resources, and flowering pattern can limit fruit and seed production. Open pollination and pollen supplementation studies are used to estimate any pollen limitation in natural populations. To study the impact of these factors on the reproductive success of Vriesea gigantea, an epiphytic
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... Annette Droste 1 *; Paulo César Pimentel Leite 2 ; Giancarlo Pasquali 3 ; Elsa Cristina de Mundstock 4 ; Maria Helena Bodanese-Zanettini 5 1 ... by somatic embryogenesis is the effect of genotypes on the response of tissues on... more
... Annette Droste 1 *; Paulo César Pimentel Leite 2 ; Giancarlo Pasquali 3 ; Elsa Cristina de Mundstock 4 ; Maria Helena Bodanese-Zanettini 5 1 ... by somatic embryogenesis is the effect of genotypes on the response of tissues on induction (Komatsuda & Ohyama, 1988; Bailey et al ...
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Water deficiency decreases plant growth and productivity. Several mechanisms are activated in response to dehydration that allows plants to cope with stress, including factors controlling stomatal aperture and ramified root system... more
Water deficiency decreases plant growth and productivity. Several mechanisms are activated in response to dehydration that allows plants to cope with stress, including factors controlling stomatal aperture and ramified root system development. In addition, ABA metabolism is also implicated in the regulation of drought responses. The basic helix-loop-helix (bHLH) proteins, a large family of conserved transcription factors that regulates many cellular processes in eukaryotic organisms, are also involved in several responses that are important for plants to cope with drought stress. This review discusses distinct mechanisms related to drought-adaptive responses, especially the possible involvement of the bHLH transcription factors such as MUTE, implicated in stomatal development; RD29, an ABA-responsive gene; EGL3 and GL3, involved in thichome and root hair development; and SPT, which play roles in repressing leaf expansion. Transcription factors are potential targets for new strategies to increase the tolerance of cultivars to drought stress. Recognition of gene regulatory networks in crops is challenging, and the manipulation of bHLH genes as well as components that mediate bHLH transcription factor responses in different pathways could be essential to achieve abiotic stress tolerance in plants through genetic manipulation.
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Page 1. Research note Anther culture and cold treatment of floral buds increased symmetrical and extra nuclei frequencies in soybean pollen grains Lia R. Rodrigues1,*, Joao Marcelo S. Oliveira2, Jorge EA Mariath2, Leandro ...
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... The results suggest that the use of ethylene inhibitors dur-ing the induction process may facilitate plant recovery from ... Received: 8 October 1996 / Revision received: 6 May 1997 / Accepted: 3 June 1997 ... Genotype-specific... more
... The results suggest that the use of ethylene inhibitors dur-ing the induction process may facilitate plant recovery from ... Received: 8 October 1996 / Revision received: 6 May 1997 / Accepted: 3 June 1997 ... Genotype-specific normalization of soybean somatic embryogenesis ...