- São Paulo, Sao Paulo, Brazil
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<p>OTUs were identified by phylogenetic inference of 16S rRNA sequences and by API test system. In some cases, isolates grouped into an OTU showed different results using the API test system, whose relative proportions are shown in... more
<p>OTUs were identified by phylogenetic inference of 16S rRNA sequences and by API test system. In some cases, isolates grouped into an OTU showed different results using the API test system, whose relative proportions are shown in brackets. Only OTUs clustering more than 2 isolates are shown.</p
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A novel strictly anaerobic bacterium designated strain SSD-17B T was isolated from the hypersaline brine-sediment interface of the Shaban Deep, Red Sea. Cells were pleomorphic but usually consisted of a central coccoid body with one or... more
A novel strictly anaerobic bacterium designated strain SSD-17B T was isolated from the hypersaline brine-sediment interface of the Shaban Deep, Red Sea. Cells were pleomorphic but usually consisted of a central coccoid body with one or two “tentacle-like” protrusions. These protrusions actively alternated between a straight, relaxed form and a contracted, corkscrew-like one. A peptidoglycan layer was not detected by electron microscopy. The organism forms “fried-egg”-like colonies on MM-X medium. The organism is strictly anaerobic and halophilic and has an optimum temperature for growth of about 30 to 37°C and an optimum pH of about 7. Nitrate and nitrite are reduced; lactate is a fermentation product. The fatty acid profile is dominated by straight saturated and unsaturated chain compounds. Menaquinone 4 is the major respiratory quinone. Phylogenetic analysis demonstrated strain SSD-17B T represents a novel and distinct lineage within the radiation of the domain Bacteria . The bran...
Research Interests: Microbiology, Environmental microbiology, Electron Microscopy, Bacteriology, Biology, and 15 moreArtemia, Microbial diversity, Extremophiles, Medicine, Biological Sciences, Animals, Halophiles, Bacteria, Gram Positive Bacteria, Haloplasma, Microbial Diversity, New Genus, Molecular Sequence Data, Fatty acid profile, and Medical and Health Sciences
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Transition probability The second probability is the transition probability for a transition from to νi−1 to νi. Its distribution is generally unknown. It could be calculated with the use of simulations and density estimation methods or... more
Transition probability The second probability is the transition probability for a transition from to νi−1 to νi. Its distribution is generally unknown. It could be calculated with the use of simulations and density estimation methods or the solution of a high dimensional chemical master equation system. Both are from computational point very time consuming. Therefore, [13, 14] suggest to approximate it with a normal distribution:
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Two halophilic archaea, strains MDB25(T) and MDB20, were isolated from a sample of the brine from Lake Medee, at a depth of 3050 m, in the Mediterranean Sea. Cells of the organisms were Gram-negative, non-motile and pleomorphic, and... more
Two halophilic archaea, strains MDB25(T) and MDB20, were isolated from a sample of the brine from Lake Medee, at a depth of 3050 m, in the Mediterranean Sea. Cells of the organisms were Gram-negative, non-motile and pleomorphic, and colonies were red pigmented. Strains MDB25(T) and MDB20 showed optimum growth at 45°C, in 2.6-3.4M NaCl and at pH 7.0-8.0. The major polar lipids of the two strains were phosphatidylglycerol (PG1 and PG2), phosphatidylglycerol phosphate methyl ester (PGP-Me) and mannose-2,6-dissulfate (1→2)-glucose glycerol diether (S(2)-DGD). Menaquinone MK-8 and MK-8(H(2)) were the major respiratory quinones. The DNA G+C content of strain MDB25(T) was 63.0%. The strains were facultatively anaerobic but grew better under aerobic conditions, nitrate served as electron acceptor. Analysis of the almost complete 16S rRNA gene sequence indicated that the strains MDB25(T) and MDB20 represented a member of the genus Natrinema in the family Halobacteriaceae. Both strains formed...
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Three strains designated 221-F1(T), 221-F2 and 3030-F1 were isolated from the Matapan Vavilov Deep canyon, also known as Calypso Deep in the Eastern Mediterranean Sea, at a depth of 4,908 m. Based on 16S rRNA gene sequence analysis these... more
Three strains designated 221-F1(T), 221-F2 and 3030-F1 were isolated from the Matapan Vavilov Deep canyon, also known as Calypso Deep in the Eastern Mediterranean Sea, at a depth of 4,908 m. Based on 16S rRNA gene sequence analysis these strains were found to be most closely related to Palleronia marisminoris and Hwanghaeicola aestuarii, with 16S rRNA gene pairwise sequence similarity of 95.3 and 94.7 % respectively, belonging to the family Rhodobacteraceae. The strains were observed to be red-pigmented and to form non-motile cocci or pleomorphic cells. The cells were found to stain Gram-negative, to be strictly aerobic, oxidase and catalase positive. Strains 221-F1(T), 221-F2 and 3030-F1 were found to be mesophilic and to grow in medium containing up to 13 % NaCl. The major polar lipids of the three strains were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid and an unidentified aminolipid. Ubiquinone 10 (U-10) was found t...
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Research Interests: Evolutionary Biology, Microbiology, Biology, Extremophiles, Medicine, and 15 moreCape Verde, Phylogeny, Fatty acids, Halophiles, Bacteria, Soils, Phylogenetic analysis, Seawater, Soil Microbiology, Description, Idiomarina, Pseudidiomarina, Sodium Chloride, Molecular Sequence Data, and Sequence similarity
A novel strictly anaerobic bacterium designated strain SSD-17BT was isolated from the hypersaline brine-sediment interface of the Shaban Deep, Red Sea. Cells were pleomorphic but usually consisted of a central coccoid body with one or two... more
A novel strictly anaerobic bacterium designated strain SSD-17BT was isolated from the hypersaline brine-sediment interface of the Shaban Deep, Red Sea. Cells were pleomorphic but usually consisted of a central coccoid body with one or two “tentacle-like” protrusions. These protrusions actively alternated between a straight, relaxed form and a contracted, corkscrew-like one. A peptidoglycan layer was not detected by electron microscopy. The organism forms “fried-egg”-like colonies on MM-X medium. The organism is strictly anaerobic and halophilic and has an optimum temperature for growth of about 30 to 37°C and an optimum pH of about 7. Nitrate and nitrite are reduced; lactate is a fermentation product. The fatty acid profile is dominated by straight saturated and unsaturated chain compounds. Menaquinone 4 is the major respiratory quinone. Phylogenetic analysis demonstrated strain SSD-17BT represents a novel and distinct lineage within the radiation of the domain Bacteria. The branchi...
Research Interests: Microbiology, Environmental microbiology, Electron Microscopy, Bacteriology, Biology, and 15 moreArtemia, Microbial diversity, Extremophiles, Medicine, Biological Sciences, Animals, Halophiles, Bacteria, Gram Positive Bacteria, Haloplasma, Microbial Diversity, New Genus, Molecular Sequence Data, Fatty acid profile, and Medical and Health Sciences
A low-G+C, Gram-positive isolate, designated strain CVS-8(T), was isolated from a sea salt evaporation pond on the Island of Sal in the Cape Verde Archipelago. This organism was found to be a catalase- and oxidase-positive, non-motile,... more
A low-G+C, Gram-positive isolate, designated strain CVS-8(T), was isolated from a sea salt evaporation pond on the Island of Sal in the Cape Verde Archipelago. This organism was found to be a catalase- and oxidase-positive, non-motile, spore-forming, aerobic, curved rod-shaped organism with an optimum growth temperature of about 35-37 degrees C and an optimum pH between 7.5 and 8.0. Optimal growth occurred in media containing 4-6% (w/v) NaCl and no growth occurred in medium without NaCl. The cell-wall peptidoglycan was of the A1gamma type with meso-diaminopimelic acid, the major respiratory quinone was MK-7, the major fatty acids were iso-15:0, 16:0, anteiso-15:0 and iso-16:0 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminoglycophospholipid. The G+C content of the DNA was 37.9 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain CVS-8(T) represented a novel species of the genus...