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The purpose of this study was to determine the Lineweaver-Burk equation, maximum speed (μmax) and maximum specific / per hour growth rate Ks (constant saturation) of probiotic lactic acid isolates BR 12 and BR 17 with limited sucrose. The... more
The purpose of this study was to determine the Lineweaver-Burk equation, maximum speed (μmax) and maximum specific / per hour growth rate Ks (constant saturation) of probiotic lactic acid isolates BR 12 and BR 17 with limited sucrose. The material in this study was probiotic isolates of Lactic Acid Bacteria (LAB) BR 12 and BR 17 which were isolated from broiler chicken meat, determined media, and sucrose. The method used was growed probiotic LAB isolates in the media determined with a sukora concentration that wasdifferent from 0-1%. The results showed that the Lineweaver-Burk equation of the LAB probiotic BR 12 was y = 3.503x + 10.10. the maximum speed (μmax) LAB probiotic BR 12 was0.0075 hour-1 and the constant Michaelis-Menten (KS) was 0.264g / 100 ml. Lineweaver-Burk equation of LAB probiotic BR 17 was y = 2.809x + 11.40. The maximum speed (μmaks) of LAB probiotics BR 17 was 0.088 / hour and the Michaelis-Menten constant (Km) = 0.2464 g / 100ml. The conclusion of this study was ...
Research Interests: Chemistry, Food Science, Poultry Meat, EAI, Bacteria, and 4 moreFermentation, Probiotic, Lactic Acid, and Broiler
ABSTRACT: The experiment was done to evaluate the influence of robusta coffee pulp fermented by rumen cellulolytic bacteria on in vitro gas production. The first step of this experiment was cellulolytic inoculum production by using fluid... more
ABSTRACT: The experiment was done to evaluate the influence of robusta coffee pulp fermented by rumen cellulolytic bacteria on in vitro gas production. The first step of this experiment was cellulolytic inoculum production by using fluid fermentation with cellulose as substrat. The inoculum produced was then used for coffee pulp fermentation. Cellulolytic bacteria was added into 200 g coffee pulp as much as 0%, 5% and 10% based on dry matter. Each treatment had three replicates. Fermentation was carried out at room temperature during 21 days anaerobically. At the end of ermentation, samples were taken out for nutrient determination including physical, chemical qualities, and in vitro gas production. Data obtained were analyzed by one way design and continued by Duncan’s new multiple range test to examine the differences between mean values. The results showed that 5% and 10% cellulolytic bacteria addition decreased pH value, and crude fiber (CF) content as much as 12.89% and 16.32% ...
Research Interests: Food Science, Biology, Bacteria, Fermentation, Dry Matter, and 3 moreRumen, Beet Pulp, and Coffea Canephora
A bstrak Tujuan penelitian ini adalah untuk mengetahui persentase hasil sosis ayam fermentasi probiotik dengan berbagai bahan aditif pada tahap fermenatsi dan tahap pengeringan. Bahan yang digunakan adalah daging ayam dan lemak sapi, ... more
A bstrak Tujuan penelitian ini adalah untuk mengetahui persentase hasil sosis ayam fermentasi probiotik dengan berbagai bahan aditif pada tahap fermenatsi dan tahap pengeringan. Bahan yang digunakan adalah daging ayam dan lemak sapi, garam, nitrit (NaNO 2 ), gula pasir, lada, bawang putih, selongsong sosis, starter BAL probiotik yaitu Lactobacillus fermentum BR 17. Perlakuan pada penelitian ini adalah sosis fermentasi dengan bahan aditif : nitrit, BAL Probiotik dan BAL Probiotik - nitrit. Parameter yang diamati adalah persentase hasil Sosis ayam fermentasi pada tahap fermentasi dan tahap pengeringan . Data yang diperoleh dari perlakuan kemudian dianalisis variansi menggunakan Rancangan Petak Terbagi (Split Plot Design). Jika ada perbedaan dilanjukan dengan uji Duncan’s New Multiple Range Test. Hasil dari penelitian ini adalah Bahan aditif yang digunakan berpengaruh sangat nyata (P<0,0 1 ) terhadap persentase hasil sosis ayam fermentasi probiotik pada tahap fermentasi...
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This research was conducted to determined the effect of fermentation duration of aren stem waste on chemical composition and in vitro digestibility. In the beginning of fermentation, 0.3 g biostaterand 1.2 g urea was added to 300 g aren... more
This research was conducted to determined the effect of fermentation duration of aren stem waste on chemical composition and in vitro digestibility. In the beginning of fermentation, 0.3 g biostaterand 1.2 g urea was added to 300 g aren stem waste. Fermentation was conducted anaerobically at room temperature for 10, 20, 30 40 d with three replicate for each treatment. The samples of fermented aren stem waste were analyzed physical quality 9texture, odor, color, growth of fungi and PH), chemical composition, and in vitro digestibility. The data obtained were analyzed variance following one way design, and continued by Dunchan’s new multiple range test for any differance between treatments. Result showed that fermentation gave soft texture, more acidic odor, and dark brown color than fresh aren stem waste. The fermentatin decreased pH of aren steam waste up to less than 4,2 (P,0.01) at 20, 30 and 40 d (4.2. 3.82 and 4.17). The chemically composition of fermented aren stem waste increa...
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To anticipate the problem of forage shortage during dry season in Indonesia, the usage of unconventional feedstuffs such as agricultural crop residues (Utomo, 2015) and livestock waste as feed is one of the strategies that can be applied.... more
To anticipate the problem of forage shortage during dry season in Indonesia, the usage of unconventional feedstuffs such as agricultural crop residues (Utomo, 2015) and livestock waste as feed is one of the strategies that can be applied. This strategy may optimize the use of natural resources for the benefit of livestock production in concern with environment sustainability (Anonymous, 1996). One of livestock waste that can be used as a feed substitute for forage basal feed is rumen contents from slaughter houses, which until now was discarded or only used as organic fertilizer. The increase of slaughtered cattle to meet the needs of meat was followed by raising amount of rumen contents, which causing pollution problems in urban areas. Each cattle slaughtered would produce about 24.5 kg of fresh rumen contents or 3.8 kg in dry matter, since they contain 15.5% dry matter (Witherow and Lammers, 1976). The use of rumen contents from a slaughter house as feed for cattle have been repor...
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Gliricidia maculata which has long been used as a forage for animal feed may contain a variety of glucogenic amino acids variants. The objective of this study was to identify the glucogenic amino acids content in Gliricidia maculata as an... more
Gliricidia maculata which has long been used as a forage for animal feed may contain a variety of glucogenic amino acids variants. The objective of this study was to identify the glucogenic amino acids content in Gliricidia maculata as an alternative source of energy for high-yielding periparturient dairy cows. The samples were the edible portion of plants, harvested randomly at the optimal cutting age (80 days), from the feed plant collection garden of The Faculty of Animal Science, Universitas Gadjah Mada. Lyophilization was carried out by drying the samples at 55°C for 3 x 24 hours continouesly in a Sanyo Drying Oven MOV-112. Pulverization was done by the Foss Tecator CyclotecTM 1093 Sample Mill with 300 mesh (1 mm screen). The hydrolysis of amino acid was carried out using HCl solution while amino acid derivatization used O-phtalaldehyde (OPA) solution. Separation, determination, and quantification of amino acid were carried out by an analytical method in gradient elution using ...
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This experiment was aimed to study the effect of nutmeg leaf tannin addition on in vitro nutrient digestibility. Treatments in this experiment consisted of: P0 (control without tannin), P1 (feed + 2% tannin) and P2 (feed + 4% tannin).... more
This experiment was aimed to study the effect of nutmeg leaf tannin addition on in vitro nutrient digestibility. Treatments in this experiment consisted of: P0 (control without tannin), P1 (feed + 2% tannin) and P2 (feed + 4% tannin). Feed for fermentation substrate consisted of Pennisetum purpureum and soybean meal with ratio 60:40. Fermentation was carried out using Tilley and Terry two stages in vitro technique for 48 hours. Variables measured were the digestibility of dry matter, organic matter, and crude protein in the rumen as well as the total digestive tract digestibility based on in vitro technique. The data obtained were analyzed by One Way ANOVA, and followed by the Duncan Multiple Range Test (DMRT). The results showed that rumen dry matter digestibility was lower (P<0.05) in P1 and P2 (59.03±3.24 and 57.19±1.32) compared to P0 (70.77±1.05), but did not show a significant difference (P>0.05) in the total dry matter digestibility of P0, P1, and P2 (74.88±5.28, 67.70±...
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The current study was aimed to evaluate the ability of phenol content in cashew nutshell liquid (CNSL) in binding protein and its effect on in vitro nutrient digestibility. This research divided into three steps, there were determination... more
The current study was aimed to evaluate the ability of phenol content in cashew nutshell liquid (CNSL) in binding protein and its effect on in vitro nutrient digestibility. This research divided into three steps, there were determination of total phenol and tannins content in CNSL, optimalization binding of CNSL tannin to bovine serum albumin with the determination of protein-percipitable phenolics compound and protein content using Lowry method, after that amount of optimalization supplementation of CNSL was used for measuring on in vitro nutrient digestibility by Tilley and Terry method. The results showed that phenol and tannin content of CNSL were 148.69 g/100 ml and 28.3 g/100 ml. One gram tannin in CNSL could be bound by 65.83 g protein. Optimum tannin were used in this study was 5.85 g tannin or equivalent to 163.91 mg of CNSL. Amount of CNSL were used in various levels, there are 0, 50, 100, 150 dan 200 mg. CNSL supplementation reduced dry matter, organic matter crude protei...
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This study was conducted to determine effectiveness of inoculants consisted of lactic acid bacteria Lactobacillus plantarum (Lp) and yeast Saccharomyces cerevisiae (Sc) combined with addition of rice bran on the physico-chemical... more
This study was conducted to determine effectiveness of inoculants consisted of lactic acid bacteria Lactobacillus plantarum (Lp) and yeast Saccharomyces cerevisiae (Sc) combined with addition of rice bran on the physico-chemical characteristics and palatability of king grass (Pennisetum hybrid) silage. The experiment was arranged on the factorial randomized design (3x3) consisting of the inoculants treatments (control, Lp, Lp+Sc) and the addition level of rice bran (0, 5 and 10%). The measured variables were physico-chemical characteristics i.e. colour, odour, pH, lactic acid, Fleigh points, and palatability of silage. Inoculation of Lp and Lp+Sc improved silage odour and reduced fungal contamination. Silage was treated by Lp+Sc and rice bran (5-10%) showed reduction of pH and an increase of lactic acid and Fleigh points. However, interaction between inoculants and rice bran treatment was not significance. Either inoculation or addition of rice bran tended to enhance the palatabilit...
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This study was aimed to obtain oil and the exact saponification with different NaOH concentration to protect unsaturated fats, which does can result in good production performance and lamb meat quality with low saturated fatty acid. Stage... more
This study was aimed to obtain oil and the exact saponification with different NaOH concentration to protect unsaturated fats, which does can result in good production performance and lamb meat quality with low saturated fatty acid. Stage one studied the performance of sheep production on supplementing lemuru fish oil (LFO) protected with different saponification optimization. Twenty lambs aged 5-6 months early weighing 8-14 kg were divided into 4 treatments, namely P0 basal feed (50% elephant grass + 50% concentrate), P1 (basal feed + soap LFO NaOH 10%), P2 (basal feed + soap LFO NaOH 20%) and P3 (basal feed + soap LFO NaOH 30%) with completely randomized design and 5 replication for performance and 3 replication for meat quality. The results showed that the treatment effect was not significant (P>0.05) on the consumption of dry matter (DM), crude protein (CP), ether extract (EE), total digestible nutrien (TDN), daily gain and blood cholesterol. P2 yield the highest daily gain 1...
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The aim of this study was to determine the levels of free fatty acids and carboxymethylcellulase activity (cmc-ase) activity of some protected fat-proteins base on in vitro Tilley and Terry method. Two sources of fat, i.e. crude palm oil... more
The aim of this study was to determine the levels of free fatty acids and carboxymethylcellulase activity (cmc-ase) activity of some protected fat-proteins base on in vitro Tilley and Terry method. Two sources of fat, i.e. crude palm oil and fish oil and three sources of protein i.e. skim milk, soybean flour and soybean meal were used in the formulation of protected fat-protein, and thus there were six treatment combinations. The filtrate from the in vitro test was analyzed for the levels of free fatty acids and cmcase activity. The result of this research indicates that different combinations of feed materials and fat give different content of free fatty acid in first stage and second stage in vitro, with the best results in the combination treatment of skim milk and palm oil that give the lowest result of free fatty acid concentration in fisrt stage in vitro (0.168%) and the highest result free fatty acid concentration in second stage in vitro ( 4.312%) . The activity of CMC-ase...