Research Interests: Andrology, Biology, Medicine, Humans, Fertility and Sterility, and 15 moreFemale, Ovulation, Ovulation Induction, Obstetrics and gynecology, Aneuploidy, Blastocyst, Menstrual Cycle, Embryo Transfer, Clinical Sciences, Adult, Ovarian Reserve, Cohort Studies, Ovarian stimulation, Follicular phase, and Luteal phase
Research Interests: Rehabilitation, Medicine, Pregnancy, Population, Humans, and 15 moreFemale, Obstetrics and gynecology, Embryo Transfer, Reproductive medicine, Preimplantation genetic diagnosis, Human reproduction, Adult, Maternal Age, Vitrification, Retrospective Studies, Fertilization in Vitro, Multiple Pregnancy, Advanced Maternal Age, Live birth, and Medical and Health Sciences
Production of transgenic animals via somatic cell nuclear transfer (SCNT) has been adapted worldwide, but this application is somewhat limited by its relatively low efficiency. In this study, we used handmade cloning (HMC) established... more
Production of transgenic animals via somatic cell nuclear transfer (SCNT) has been adapted worldwide, but this application is somewhat limited by its relatively low efficiency. In this study, we used handmade cloning (HMC) established previously to produce transgenic pigs that express the functional nematode fat-1 gene. Codon-optimized mfat-1 was inserted into eukaryotic expression vectors, which were transferred into primary swine donor cells. Reverse transcriptase PCR (RT-PCR), gas chromatography, and chromosome analyses were performed to select donor clones capable of converting n-6 into n-3 fatty acids. Blastocysts derived from the clones that lowered the n-6/n-3 ratio to approximately 1:1 were transferred surgically into the uteri of recipients for transgenic piglets. By HMC, 37% (n=558) of reconstructed embryos developed to the blastocyst stage after 7 days of culture in vitro, with an average cell number of 81±36 (n=14). Three recipients became pregnant after 408 day-6 blasto...
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Technology of somatic cell nuclear transfer (SCNT) has been adapted worldwide to generate transgenic animals, although the traditional procedure relies largely on instrumental micromanipulation. In this study, we used the modified... more
Technology of somatic cell nuclear transfer (SCNT) has been adapted worldwide to generate transgenic animals, although the traditional procedure relies largely on instrumental micromanipulation. In this study, we used the modified handmade cloning (HMC) established in cattle and pig to produce transgenic sheep with elevated levels of omega-3 (n-3) fatty acids. Codon-optimized nematode mfat-1 was inserted into a eukaryotic expression vector and was transferred into the genome of primary ovine fibroblast cells from a male Chinese merino sheep. Reverse transcriptase PCR, gas chromatography, and chromosome analyses were performed to select nuclear donor cells capable of converting omega-6 (n-6) into n-3 fatty acids. Blastocysts developed after 7 days of in vitro culture were surgically transplanted into the uterus of female ovine recipients of a local sheep breed in Xinjiang. For the HMC, approximately 8.9% (n =925) of reconstructed embryos developed to the blastocyst stage. Four recip...
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Research Interests: Andrology, Photography, Biology, Medicine, Embryo, and 15 moreMice, In Vitro Fertilisation, Female, Animals, Fragmentation, Blastocyst, Embryo Transfer, Embryonic Development, In vitro culture, Embryos, Embryogenesis, Development Time, Predictive value of tests, Embryo Development, and Paediatrics and reproductive medicine
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Research Interests: Biology, Cryopreservation, Infertility, Medicine, Humans, and 15 moreFertility preservation, Female, Obstetrics and gynecology, Embryo Transfer, Longitudinal Studies, Human reproduction, Adult, Odds ratio, Fertilization in Vitro, Female Age, Oocyte, Confidence Interval, Cohort Studies, Medical and Health Sciences, and Delivery rate
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Research Interests: Biology, Cell Biology, Cryopreservation, Medicine, Embryo, and 15 moreBiological Sciences, Cell Differentiation, Humans, Germ Cells, Female, Animals, Male, Osmotic pressure, Embryonic Stem Cells, Embryonic Development, Physiological Stress Markers, Hydrostatic Pressure, Biology Reproduction, ovum, and Medical and Health Sciences
Background: Twenty five years after the first successful application in mammalian embryology vitrification seems to emerge now from the “new”, “experimental”, “immature” category and has become a method of choice for routine use both in... more
Background: Twenty five years after the first successful application in mammalian embryology vitrification seems to emerge now from the “new”, “experimental”, “immature” category and has become a method of choice for routine use both in domestic animals and humans. Review: The aim of this review is to summarize major steps of this regretfully slow development, outline the actual possibilities and limitations, highlight some promising perspectives, and deal also with factors that have hampered and still hamper the widespread application. The unique feature of vitrification in cryobiology is the total elimination of ice formation both in the sample and the entire solution surrounding it. This radical approach requires some drastic measures including high cryoprotectant concentration and high cooling/warming rates, although theoretically none of these factors are absolute requirements for vitrification. Initial efforts to improve vitrification results were focused on decreasing the tox...