Peter Dunkley

Project CALIPSO (Caribbean Andesite Lava Island Precision Seismo-geodetic Observatory) aims to investigate the magmatic system at the active Soufriere Hills Volcano (SHV), Montserrat. The collaborative project involves several institutions acting in partnership with the Montserrat Volcano Observatory (MVO), and is funded by NSF with assistance by NERC. SHV remains active after 9 years, displaying cyclic activity on several scales. Many aspects of andesite system dynamics remain poorly understood, and CALIPSO is expected to improve our understanding of SHV and andesite systems generally. Drilling was carried out Nov 02 to Mar 03. CALIPSO comprises an integrated array of four strategically located 200-m boreholes, plus several shallower holes and surface installations. The borehole instruments are designed to have long life (decades). Each site includes a very broad-band Sacks-Evertson strainmeter, three-component seismometer, tiltmeter, and surface cGPS station. At one site a hot-hole strainmeter design, involving hydraulic sensors and no downhole electronics, has been used for the first time anywhere. FreeWave telemetry is coupled with Quanterra A/D converters. These instruments are intended to probe changes in the andesitic volcanic system and underlying mafic sources with unprecedented sensitivity. Early data from the July 2003 dome collapse suggest remarkable insights about the depth, shape and nature of the volatile-saturated magmatic reservoir, gleaned from the magnitude of dilatation pulses accompanying the collapse, and their change in sign of with radial distance.

Soufriere Hills Volcano, Montserrat, Lesser Antilles, has been undergoing a lava dome forming eruption since 1995. A major collapse of the dome occurred on 12-13 July 2003 and was accompanied by explosive activity. This was the largest dome-collapse to date at Soufriere Hills Volcano. The collapse was a prolonged event. Continuous pyroclastic flows began in the Tar River Valley on the eastern side of the volcano at 09:30 local time 12 July. From 10.45 onwards these reached the sea and at 18:30 the flows became larger and more energetic as the collapse progressively cut back into the hotter interior of the dome. The collapse reached its most energetic phase between 21.50 12 July and 0.50 13 July when a sequence of very large pyroclastic flows entered the sea and pyroclastic surges traveled up to 3 km across the surface of the sea. The climax of the collapse occurred at 23.35 when a very large pyroclastic flow impacted the sea and pyroclastic surges devastated 10 km22 on the NE flank ...

Project CALIPSO (Caribbean Andesite Lava Island-volcano Precision Seismo-geodetic Observatory) was developed in order to investigate the magmatic system at the exceedingly active Soufriere Hills Volcano (SHV), Montserrat. The collaborative project involves a number of institutions acting in partnership with the Montserrat Volcano Observatory (MVO), and is funded by NSF with a contribution to drilling costs provided by UK NERC. SHV

... Dunkley, P.; Voight, B.; Edmonds, M.; Herd, R.; Strutt, M.; Thompson, G.; Bass, V.; Aspinall, WP; Neuberg, J.; Sparks, R.; Mattioli, G.; Hidayat, D.; Elsworth ... One of the regular six-monthly meetings of the Risk Assessment Panel (RAP) took place on 3-4 Sept 02 and concluded that if ...

In cells from the adrenal medulla, angiotensin II (AII) regulates both the activity and mRNA levels of catecholamine biosynthetic enzymes whose expression is thought to be under the control of cAMP-responsive element (CRE) binding protein (CREB). In this study, we evaluated the effect of AII stimulation on CREB phosphorylation at Ser133 (pCREB) in bovine adrenal chromaffin cells (BACC). We found that AII produces a rapid and AII type-1 receptor (AT1)-dependent increase in pCREB levels, which is blocked by the MEK1/2 inhibitor U0126 but not by H-89, SB203580 or KN-93, suggesting that it is mediated by the extracellular-regulated protein kinases 1 and 2 (ERK1/2) and not by cAMP-dependent protein kinase (PKA), p38 mitogen-activated protein kinase (p38MAPK) or Ca(2+)/calmodulin-dependent protein kinases (CaMKs) dependent pathways. Gel-shift experiments showed that the increase in pCREB levels is accompanied by an ERK1/2-dependent upregulation of CRE-binding activity. We also found that AII promotes a rapid and reversible increase in the activity of the non-receptor tyrosine kinase Src and that the inhibition of this enzyme completely blocks the AII-induced phosphorylation of ERK1/2, the CREB kinase (p90)RSK and CREB. Our data support the hypothesis that in BACC, AII upregulates CREB functionality through a mechanism that requires Src-mediated activation of ERK 1/2 and (p90)RSK.

Tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis, is regulated acutely by protein phosphorylation and chronically by protein synthesis. No studies have systematically investigated the phosphorylation of these sites in vivo in response to stressors. We specifically investigated the phosphorylation of TH occurring within the first 24 h in response to the social defeat stress in the rat adrenal, the locus coeruleus, substantia nigra and ventral tegmental area. Five groups were investigated; home cage control (HCC), two groups that underwent social defeat (SD+) which were sacrificed either 10 min or 24 h after the end of the protocol and two groups that were put into the cage without the resident being present (SD-) which were sacrificed at time points identical to the SD+. We found at 10 min there were significant increases in serine 40 and 31 phosphorylation levels in the locus coeruleus in SD+ compared to HCC and increases in serine 40 phosphorylation levels in the substantia nigra in SD+ compared to SD-. We found at 24 h there were significant increases in serine 19 phosphorylation levels in the ventral tegmental area in SD+ compared to HCC and decreases in serine 40 phosphorylation levels in the adrenal in SD+ compared to SD-. These findings suggest that the regulation of TH phosphorylation in different catecholamine-producing cells varies considerably and is dependent on both the nature of the stressor and the time at which the response is analysed.

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in catecholamine synthesis. Its activity is controlled by PACAP, acutely by phosphorylation at Ser40 and chronically by protein synthesis. Using bovine adrenal chromaffin cells we found that PACAP, acting via the continuous activation of PACAP 1 receptors, sustained the phosphorylation of TH at Ser40 and led to TH activation for up to 24 h in the absence of TH protein synthesis. The sustained phosphorylation of TH at Ser40 was not mediated by hierarchical phosphorylation of TH at either Ser19 or Ser31. PACAP caused sustained activation of PKA, but did not sustain activation of other protein kinases including ERK, p38 kinase, PKC, MAPKAPK2 and MSK1. The PKA inhibitor H89 substantially inhibited the acute and the sustained phosphorylation of TH mediated by PACAP. PACAP also inhibited the activity of PP2A and PP2C at 24 h. PACAP therefore sustained TH phosphorylation at Ser40 for 24 h by sustaining the activation of PKA and causing inactivation of Ser40 phosphatases. The PKA activator 8-CPT-6Phe-cAMP also caused sustained phosphorylation of TH at Ser40 that was inhibited by the PKA inhibitor H89. Using cyclic AMP agonist pairs we found that sustained phosphorylation of TH was due to both the RI and the RII isotypes of PKA. The sustained activation of TH that occurred as a result of TH phosphorylation at Ser40 could maintain the synthesis of catecholamines without the need for further stimulus of the adrenal cells or increased TH protein synthesis.

... And his opinion later took on some theoretical ballast from the work of Garrett Hardin, who argued that the end of common right was due to the inevitable over-use of common lands, itself an economically logical result of sharing property rights in common.7 The argument that ...