There is much evidence suggesting that mitigating the COVID-19 pandemic requires syndromic surveillance and isolation of suspected/confirmed cases. The availability of diagnostic tests in the Brazilian Unified Health System (SUS) is... more
There is much evidence suggesting that mitigating the COVID-19 pandemic requires syndromic surveillance and isolation of suspected/confirmed cases. The availability of diagnostic tests in the Brazilian Unified Health System (SUS) is essential, which could be facilitated by national sovereignty in development and production, considering the high demand/lack of supply in the international market. This study identified the stages of translational research into diagnostic tests for COVID-19 in Brazil, verifying their geographic distribution, among other indicators. A cross-sectional, exploratory study based on a public database with 789 Research, Development, and Innovation (RD&I) projects regarding COVID-19, complemented by other searches, including the researchers’ curricula (CVLattes). There were 89 diagnostic test projects in the database. In 45 cases, it was possible to obtain additional information to classify them according to the translational research stages. Fifteen innovation...
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RESUMO Existe ampla evidência que a contenção da pandemia de Covid-19 requer vigilância sindrômica e isolamento de casos suspeitos/confirmados. É essencial a disponibilidade de testes diagnósticos no Sistema Único de Saúde, que poderia... more
RESUMO Existe ampla evidência que a contenção da pandemia de Covid-19 requer vigilância sindrômica e isolamento de casos suspeitos/confirmados. É essencial a disponibilidade de testes diagnósticos no Sistema Único de Saúde, que poderia ser facilitada pela soberania nacional no desenvolvimento e produção, considerando-se a alta demanda/escassez no mercado internacional. Este estudo identificou as etapas da pesquisa translacional de testes diagnósticos para Covid-19 no Brasil, verificando sua distribuição geográfica, entre outros indicadores. Estudo transversal, exploratório, partindo de banco público com 789 projetos de Pesquisa, Desenvolvimento e Inovação (PD&I) em Covid-19, complementado com outras buscas, inclusive no CVLattes dos pesquisadores. No banco, havia 89 projetos de testes diagnósticos. Em 45 casos, foi possível obter informações complementares para classificá-los conforme as etapas da pesquisa translacional. Identificaram-se 15 inovações que atingiram o estágio T3, ou s...
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Aims: The present study aimed to identify miRNAs differentially expressed in rats submitted to strenuous exercise and in silico investigation of the biological implication of the findings. Place and Duration of Study: The in vivo... more
Aims: The present study aimed to identify miRNAs differentially expressed in rats submitted to strenuous exercise and in silico investigation of the biological implication of the findings. Place and Duration of Study: The in vivo experiments and analyses were performed in the Laboratory of Biochemistry and Gene Expression – LABIEX of the Superior Institute of Biomedical Science – ISCB from the State University of Ceará. Between 2017-2020. Methodology: The study was performed using as subjects 2-month-old male wistar rats, which initially were submitted to 2-week adaptation training. Later the animals were separated in two distinct groups, control (C) and trained (T), where only T performed a single session of strenuous exercise, while C were not submitted to this treatment. The applied exercise protocol consisted in a running training in treadmill with speed constant increasing until the animal exhaustion which was measured by the animal refusal to keep running. After 24h, soleus m...
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Research Interests: Humanities and Biology
Alzheimer's disease (AD) is the most common form of dementia, involving a progressive loss of synapses in different regions of the central nervous system (CNS), with the onset in the hippocampus, with cognitive dysfunction and... more
Alzheimer's disease (AD) is the most common form of dementia, involving a progressive loss of synapses in different regions of the central nervous system (CNS), with the onset in the hippocampus, with cognitive dysfunction and extensive neuronal loss. Animal models are often used in research to discover novel pharmacological treatments, but have limitations as long study times and less controlled variables related to environmental factors. The use of hippocampal subgranular and subventricular zone stem cells obtained from transgenic animals for AD cultivated in vitro may allow the better understating of the molecular mechanisms involved in the disease and to minimize the research timeframe. Epidemiological and genetic studies have decumented the involvement of genetic risk factors to acquire AD, such as the Apolipoprotein E gene. Thus, the possible use of neural stem cells in promoting neurogenesis and repairing the affected brain areas to improve neuronal loss is key for AD tre...
Translational research involves the interface between basic research and medical practice in order to generate innovative products or processes to introduce them into clinical protocols and health systems. The objective of this essay was... more
Translational research involves the interface between basic research and medical practice in order to generate innovative products or processes to introduce them into clinical protocols and health systems. The objective of this essay was to present an overview of transcriptomic advances, subsidized by the availability and use of new information technologies and molecular biology. In the search for accurate and less invasive diagnosis, transcriptomic tests use gene expression signatures to detect neurodegenerative diseases (Parkinson and Alzheimer), autoimmune (systemic lupus erythematosus, Wegener’s granulomatosis), heart failure, autism and cancer (breast, colorectal, hepatic and lung). In the English health system the clinical guidelines incorporate eight transcriptomic tests, all with a focus on cancer. In Brazil genomic tests based on DNA sequences are regulated to diagnose congenital anomalies both in the Unified Health System and in supplementary health, but the molecular test...
RESUMO A pesquisa translacional envolve a interface entre a pesquisa básica e a clínica médica com o intuito de gerar produtos ou processos inovadores para introduzi-los nos protocolos clínicos e nos sistemas de saúde. O objetivo desse... more
RESUMO A pesquisa translacional envolve a interface entre a pesquisa básica e a clínica médica com o intuito de gerar produtos ou processos inovadores para introduzi-los nos protocolos clínicos e nos sistemas de saúde. O objetivo desse ensaio foi apresentar uma visão geral dos avanços da transcriptômica, subsidiados pela disponibilidade e utilização das novas tecnologias da informação e biologia molecular. Na busca pelo diagnóstico preciso e menos invasivo, testes transcriptômicos utilizam assinaturas de expressão gênica visando detectar doenças neurodegenerativas (Parkinson e Alzheimer), autoimunes (lúpus eritematoso sistêmico, granulomatose de Wegener), insuficiência cardíaca, autismo e câncer (de mama, colorretal, hepático e de pulmão). No sistema de saúde inglês as diretrizes clínicas incorporam oito testes transcriptômicos, todos com foco no câncer. No Brasil testes genômicos com base nas sequências de DNA são regulamentados para diagnosticar anomalias congênitas, tanto no Sist...
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RESUMO Introdução: Novos estudos de regulação gênica do exercício físico por meio de técnicas pós-genômicas em ensaios de resistência (endurance) e força caracterizam a transcriptômica do exercício físico. Entre os genes afetados,... more
RESUMO Introdução: Novos estudos de regulação gênica do exercício físico por meio de técnicas pós-genômicas em ensaios de resistência (endurance) e força caracterizam a transcriptômica do exercício físico. Entre os genes afetados, destacamos a via da proteína quinase ativada por AMP (AMPK), cuja ativação ocorre durante o exercício como resultado das alterações dos níveis de fosfato energético da fibra muscular. Objetivo: Avaliar a via de sinalização da AMPK por revisão sistemática da expressão de genes e análise in silico. Método: Foi efetuada uma revisão sistemática para avaliar a regulação gênica da via de sinalização AMPK, caracterizando os genes estudados na literatura, as variações de regulação obtidas, na forma de fold change e tipos de exercício usados. Resultados: A via de sinalização AMPK mostrou 133 genes no repositório KEGG (Kyoto Encyclopedia of Genes and Genomes), os quais foram confrontados com a revisão sistemática da literatura, totalizando 65 genes. Dezessete genes ...
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Historicamente, a Biologia sempre necessitou de bancos de dados. O desenvolvimento da internet permitiu que uma grande quantidade de informações biológicas armazenadas, sobretudo no nível molecular, estejam disponíveis gratuitamente para... more
Historicamente, a Biologia sempre necessitou de bancos de dados. O desenvolvimento da internet permitiu que uma grande quantidade de informações biológicas armazenadas, sobretudo no nível molecular, estejam disponíveis gratuitamente para a utilização, tanto por profissionais da saúde, como por educadores. Nesse contexto, apresentamos uma proposta, direcionada a universitários de cursos ligados às ciências biológicas e biomédicas, em que os participantes são motivados a desvendar um mistério médico a partir de uma fotonovela, e a utilizar as ferramentas de busca e análise bioinformática para desvendá-lo. A atividade contém um roteiro que visa orientar a navegação em alguns dos principais bancos de dados biológicos – OMIM, NCBI gene, PubMed, dbSNP e Ensembl – fornecendo links e caminhos até as informações necessárias para a finalização da tarefa. Para ilustrar os procedimentos, utilizamos uma simulação envolvendo a síndrome de Berardinelli-Seip, escolhida por ser uma doença ligada a p...
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Background The coronavirus disease 2019 (COVID-19) pandemic has spread throughout more than 160 countries, infecting millions of people worldwide. To address this health emergency, countries have organized the flow of production and... more
Background The coronavirus disease 2019 (COVID-19) pandemic has spread throughout more than 160 countries, infecting millions of people worldwide. To address this health emergency, countries have organized the flow of production and innovation to reduce the impact on health. This article shows the response of the Brazilian scientific community to meet the urgent needs of the public unified health system [SUS], aiming to guarantee universal access to an estimated population of 211 million. By December 2020, Brazil had recorded more than six million cases and approximately 175,000 deaths. Methods We collected data on research, development and innovation projects carried out by 114 public universities (plus Oswaldo Cruz Foundation [Fiocruz] and Butantan Institute), as reported on their websites. Additionally, we examined the studies on COVID-19 approved by the National Comission for Research Ethics, as well as those reported on the Ministry of Education website as of May 15, 2020. Resu...
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Background Physical exercise is a health promotion factor regulating gene expression and causing changes in phenotype, varying according to exercise type and intensity. Acute strenuous exercise in sedentary individuals appears to induce... more
Background Physical exercise is a health promotion factor regulating gene expression and causing changes in phenotype, varying according to exercise type and intensity. Acute strenuous exercise in sedentary individuals appears to induce different transcriptional networks in response to stress caused by exercise. The objective of this research was to investigate the transcriptional profile of strenuous experimental exercise. Methodology RNA-Seq was performed with Rattus norvegicus soleus muscle, submitted to strenuous physical exercise on a treadmill with an initial velocity of 0.5 km/h and increments of 0.2 km/h at every 3 min until animal exhaustion. Twenty four hours post-physical exercise, RNA-seq protocols were performed with coverage of 30 million reads per sample, 100 pb read length, paired-end, with a list of counts totaling 12816 genes. Results Eighty differentially expressed genes (61 down-regulated and 19 up-regulated) were obtained. Reactome and KEGG database searches rev...
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O sequenciamento do genoma humano proporcionou o aprofundamento de diversos tipos de estudos e tecnologias de análise biológica, dentre estas, o microarranjo. A necessidade publicar os dados brutos dessas pesquisas impulsionou a criação... more
O sequenciamento do genoma humano proporcionou o aprofundamento de diversos tipos de estudos e tecnologias de análise biológica, dentre estas, o microarranjo. A necessidade publicar os dados brutos dessas pesquisas impulsionou a criação de bancos de dados públicos onde essas informações pudessem ser indexadas e resgatadas. Essas bases são uma grande fonte de dados transcriptômicos que infelizmente acabam sendo subutilizadas. O objetivo deste trabalho foi o desenvolvimento de um sistema WEB para mineração de dados em estudos transcriptômicos a partir de microarranjos armazenados no banco de dados biológico Gene Expression Omnibus (GEO), o Mining_RNA. Através de uma usabilidade passo-a-passo juntamente com uma série de filtros o sistema possibilita resgatar dados do GEO, calcular a expressão diferencial entre os genes de um estudo, possibilitando ainda análises estatísticas para cada gene do estudo analisado. O sistema foi validado através da comparação com a avaliação dos mesmos dado...
Research Interests: Physics and Humanities
O ensino de biologia molecular e complexo, e e necessario aplicar tecnologias de uma forma contextualizada, de modo a auxiliar no processo ensino-aprendizagem. Bancos de dados biologicos sao ferramentas para compartilhar dados de... more
O ensino de biologia molecular e complexo, e e necessario aplicar tecnologias de uma forma contextualizada, de modo a auxiliar no processo ensino-aprendizagem. Bancos de dados biologicos sao ferramentas para compartilhar dados de pesquisas cientificas e abrangem varios conteudos, como sequencias de nucleotideos e estrutura de proteinas. O trabalho visa relatar uma experiencia na monitoria, com a elaboracao e aplicacao de uma guia para o uso de bancos de dados nas disciplinas biologia molecular e biotecnologia (UECE). Os alunos consideraram os recursos relevantes para as disciplinas e para sua formacao como professor. A pesquisa traz uma nova perspectiva para a inovacao das metodologias no ensino de biologia.
Research Interests: Philosophy and Humanities
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This study aimed to identify undertaken activities during Physical Education (PE) school classes, such as their content and intensity, and investigate the relationship between PE school classes and the level of physical activity of the... more
This study aimed to identify undertaken activities during Physical Education (PE) school classes, such as their content and intensity, and investigate the relationship between PE school classes and the level of physical activity of the students. Fifty school adolescents averagely aging 14.3 years, 1.63m, height and 57.4kg body mass were evaluated. Intensity of PE classes was measured by heart rate monitoring. A PE class content observation instrument was utilized in order to evaluate the sessions. Additionally, IPAQ was used to assess the level of physical activity of the participants. 20% of the students were found to be overweight considering their age. It was noticed that 78% underwent from moderate to high intensity sports activity throughout the classes, without signifi cant gender differences. 96% of the studied subjects were classifi ed as active or very active. Therefore, most of the analyzed adolescents performed satisfactory physical activity, ensuring a high level of phys...
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Regular exercise is an exogenous factor of gene regulation with numerous health benefits. The study aimed to evaluate human genes linked to physical exercise in an 'omic scale, addressing biological questions to the generated... more
Regular exercise is an exogenous factor of gene regulation with numerous health benefits. The study aimed to evaluate human genes linked to physical exercise in an 'omic scale, addressing biological questions to the generated database. Three literature databases were searched with the terms 'exercise', 'fitness', 'physical activity', 'genetics' and 'gene expression'. For additional references, papers were scrutinized and a text-mining tool was used. Papers linking genes to exercise in humans through microarray, RNA-Seq, RT-PCR and genotyping studies were included. Genes were extracted from the collected literature, together with information on exercise protocol, experimental design, gender, age, number of individuals, analytical method, fold change and statistical data. The 'omic scale dataset was characterized and evaluated with bioinformatics tools searching for gene expression patterns, functional meaning and gene clusters. As a res...
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Introduction: Diabetes mellitus is the most common metabolic disease worldwide. Endothelial dysfunction characteristic of these patients is one of the major risk factors for atherosclerosis. Early diagnosis of endothelial dysfunction is... more
Introduction: Diabetes mellitus is the most common metabolic disease worldwide. Endothelial dysfunction characteristic of these patients is one of the major risk factors for atherosclerosis. Early diagnosis of endothelial dysfunction is essential for the treatment especially of non-invasive manner, such as flow mediated dilation. Physical exercise is capable of generating beneficial adaptations may improve endothelial function. Objective: Identify the effect of physical exercise, using the clinical technique of ultrasound in the assessment of the endothelial function of patients with metabolic syndrome or type 2 diabetes mellitus. Methods: Thirty-one patients with type 2 diabetes mellitus or metabolic syndrome were studied, with a mean age (± SD) of 58±6 years, randomized into three groups. The training was performed for 50 minutes, four times a week. Before and after six weeks of training, subjects performed the endurance test and a study of the endothelial function of the brachial...