Background: Cervical and breast cancer are considered to be the most common cause of death among Iranian women. Various studies have shown that probiotics are effective fighting cancer. The aim of the present study was to investigate the... more
Background: Cervical and breast cancer are considered to be the most common cause of death among Iranian women. Various studies have shown that probiotics are effective fighting cancer. The aim of the present study was to investigate the effect of Lactobacillus brevis on apoptosis and casp (casp3, casp8) gene expression in HeLa cancer cells.
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Background. If the pregnancy attempt fails, it may become a stressful event. Therefore, in many cases, problem solving can lead to greater satisfaction and cohesion of couples and prevent psychological and social harm to families. The aim... more
Background. If the pregnancy attempt fails, it may become a stressful event. Therefore, in many cases, problem solving can lead to greater satisfaction and cohesion of couples and prevent psychological and social harm to families. The aim of this study was to determine the significant difference in the expression of miR-146a miRNA in maternal blood between the control and observation groups and to determine whether the miRNA can be used as diagnostic biomarkers or not. Methods. The present study is a basic and descriptive survey in terms of data analysis. The samples included pregnant women referred to Tabriz University of Jahad from September 2019 to February 2021. We used Exel, Spss v.21 and Roc software for data analysis. Data were prepared in Exel software with reference gene and by calculating CT. Were used in Roc software. Results. The results showed a difference between the control and observation groups. Early detection of recurrent miscarriage should be used in these mother...
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Background: Breast cancer (BC) is a malignant tumor that occurs in the epithelial tissue of the breast gland and has become the most common malignancy in women. Various studies have reported the effect of epigenetic changes, including DNA... more
Background: Breast cancer (BC) is a malignant tumor that occurs in the epithelial tissue of the breast gland and has become the most common malignancy in women. Various studies have reported the effect of epigenetic changes, including DNA methylation and microRNAs, on breast carcinogenesis. microRNAs play an important role in the post-transcriptional regulation of genes and are important regulators of oncogenic pathways. Studying microRNAs in BC facilitates the development of targeted therapies and early detection of this cancer. This study aimed to evaluate the expression level of miR-508-5p and miR-635 in BC tumor tissues compared to healthy marginal tissues.Methods and Materials: In silico analysis confirmed microarray datasets (GSE40525, GSE44124 and GSE45666) downloaded from the GEO database. The analysis was defined using the Affy packages in R software to screen remarkably dysregulated miRNAs attended by utilized to predict the potential biological processes and molecular pat...
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Aims: Leukemia is one of the most common childhood malignancies. The Epstein-Barr Virus (EBV) is a tumorigenic virus of the herpes family and causes a primary infection in young children. This study aimed to evaluate the increase in NFKB... more
Aims: Leukemia is one of the most common childhood malignancies. The Epstein-Barr Virus (EBV) is a tumorigenic virus of the herpes family and causes a primary infection in young children. This study aimed to evaluate the increase in NFKB expression following the EBV virus and its contribution to the half-life of EBV-positive acute lymphoblastic leukemia patients. Methods & Materials: In this case-control study, we examined blood samples of 60 patients referred to Tabriz Children’s Hospital for 6 months, Tabriz City, Iran, in 2019. RNA extraction was performed from the collected samples, and the quantity and quality of the extracted RNA were controlled by a NanoDrop device and gel electrophoresis. cDNA synthesis was performed from RNA extracted from the sample. NFKB gene expression was assessed using real-time PCR. The obtained data were analyzed using a t-test. Findings: The results showed that the EBV virus decreased the expression of the NFKB gene in patients with acute lymphoblas...
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Background and Objective: The emergence of drug resistance in Mycobacterium tuberculosis and especially drug resistant strains has created problems for the treatment of tuberculosis control, worldwide. This study was performed to evaluate... more
Background and Objective: The emergence of drug resistance in Mycobacterium tuberculosis and especially drug resistant strains has created problems for the treatment of tuberculosis control, worldwide. This study was performed to evaluate the changes in N-acetyltransferase (NAT) pattern of methylsonone in patients who did not respond to first-line treatment for Mycobacterium tuberculosis. Methods: This descriptive-analytical study was performed on 200 patients with tuberculosis. After preparing the blood sample, saturated salt method was used to extract DNA. For the determination of quantity and quality of DNA, two methods of spectrophotometry and electrophoresis on agarose gel were used. The methylation pattern of NAT1 gene was investigated by HRM method. Results: 34 patients were resistant to the first line of treatment. 18 patients showed hypermethylation pattern, 12 patients showed non-methylated pattern and 4 patients showed hypomethylated pattern. 166 patients were not resista...
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Tilia platyphyllos scop belongs to the Tiliaceae family and mainly grows in northern parts of the country. It has various pharmacological effects including anxiolytic, antibacterial, anticonvulsant, spasmolytic, tranquilization and... more
Tilia platyphyllos scop belongs to the Tiliaceae family and mainly grows in northern parts of the country. It has various pharmacological effects including anxiolytic, antibacterial, anticonvulsant, spasmolytic, tranquilization and sedation, hypnotic and muscular relaxation. In order to study sedative, preanaesthetic and anxiolytic effects herbal extract of Tiliaplatyphyllos scop in comparison with diazepam in different groups of female Wistar rats with the same age and weight, doses of 150 mg/kg, 300 mg/kg and 450 mg/kg of herbal extract, 1.2 mg/kg of diazepam and equal volumes of dimethyl sulfoxide as a placebo were injected to rats intraperitoneally 30 minutes prior to evaluation of sedative and preanaesthetic effects (induced sleep duration following 40 mg/kg administration intraperitoneally) and anxiolytic effects (using elevated plus maze and Rotarod test). Statistical results obtained represent a significant increase in sleep time induced with ketamine and also a significant increase in time spent by rats in open arms of maze with high and low doses of Tiliaplatyphyllos scop herbal extract (p
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Aim of this research is histopathological study of different salinity effects in the gill of Barbus sharpeyi. After a week adaptation, 120 fingerling fish were divide into 4 aquariums had the following different salinities 4, 8, 10 and 12... more
Aim of this research is histopathological study of different salinity effects in the gill of Barbus sharpeyi. After a week adaptation, 120 fingerling fish were divide into 4 aquariums had the following different salinities 4, 8, 10 and 12 g/l and one other was contained control fishes and exposed for 96 hours. Then samples of tissues were prepared and fixed in 10% formalin. After tissue processing, dehydration, clearing and blocking, 5μm sections were prepared and stained with haematoxylin and eosin and studied by light microscope. Findings showed a major change in gill structure exposed to different salinity compare with the control fish. Epithelial lifting was observed in all concentrations, but high concentrations showed more severe lesions. In these concentrations epithelial hyperplasia, increased Chloride cell and mucosal cell, epithelial lifting, mucosal cell and chloride cell hypertrophy were observed. The present results, showed that salt have toxic effects in Barbus sharpey...
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Enterococci have important role in the dairy industry. Also some of enterococcies with dairy origin have been reported as probiotics. The aim of this study is isolate and identify enterococcies from traditional dairy products of... more
Enterococci have important role in the dairy industry. Also some of enterococcies with dairy origin have been reported as probiotics. The aim of this study is isolate and identify enterococcies from traditional dairy products of Meshkinshahr and Moghan(Ardabil) regions and evaluate the potential probiotic of them. In the presence of phosphate saline buffer (PH equal to 5/2) 26 acid resistant isolates and in the presence of bile salts, 10 resistant isolates, 7 isolates with high tolerance, 6 isolates with poor tolerance and 3 susceptible isolates were identified. Biochemical identification showed isolates belonging to species of E.avium, E.faecium, E.durans and E.faecalis. Also antagonistic activity was investigated with well diffusion method showed that isolates with inhibitory ability against Escherichia coli are Yersinia enterocolitica and Listeria innocua. Susceptibility study to various antibiotics showed that all strains were susceptible to vancomycin and chloramphenicol. Also all of them were resistant to solfamethacsazol. The results indicate that traditional dairy products of these regions are important source for organisms with probiotic properties.
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Perforatum اي St. John’s wort لـگ هـب يـسراف رد هـك تـسا يهايگ مان تسا فورعم يعار . 1 نش هايگ نيا هراصع رد ييايميش تابيكرت هدـش يياسا دـنا نورتنايدوتفن زادنترابع هك نيدينايـسوتنآورپ ،اهديئونولاف ،اه نوولافيـب ،اـه ،اـه نوتنازگ لونيسولگ... more
Perforatum اي St. John’s wort لـگ هـب يـسراف رد هـك تـسا يهايگ مان تسا فورعم يعار . 1 نش هايگ نيا هراصع رد ييايميش تابيكرت هدـش يياسا دـنا نورتنايدوتفن زادنترابع هك نيدينايـسوتنآورپ ،اهديئونولاف ،اه نوولافيـب ،اـه ،اـه نوتنازگ لونيسولگ رولف ،اه نـغور ،اه لـينف و هنيمآديـسا تاقتـشم ،رارـف ياـه اهناپورپ . 2 رـضاح لاح رد Hypericum Perforatum ـب رتـشيب ه كـي ناوـنع طسوتم ات فيفخ يگدرسفا رب رثؤم هايگ تسا حرطم . لاـس رد 2001 يدلايـم
Research Interests: Pharmacology, Medicine, Sedation, Anxiolytic, Diazepam, and 3 moreE, Hypericum perforatum, and Sedative
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Humulus lupulus is a medicinal plant which in Farsi is called "razak". The purpose of this research is, studying the sedative effects of polar, semi polar and non polar fractions extracted from Hop (Humulus lupulus. L) in... more
Humulus lupulus is a medicinal plant which in Farsi is called "razak". The purpose of this research is, studying the sedative effects of polar, semi polar and non polar fractions extracted from Hop (Humulus lupulus. L) in comparison with diazepam in the animal model of Rat. For conducting this research polar, semi polar and non polar fractions extracted from Hop based on the polarity of solvent. Then study continued with the injection of obtained extracts and other medicines to different groups of Wistar breed of rats. First group was injected with 100mg/kg of Polar fraction extract the second group, with 100mg/kg of Semipolar extract, the third group, with 100 mg/kg of non-polar extract of Humulus lupulus the fourth group with 2 mg/kg of Diazepam the fifth group with the same volume of DMSO used as solvent of injectable medicines an the sixth group was the control group and did not receive any drug. The method of injection was Intra peritoneal (IP) form. Statistical diagrams and results showed a significant decreasing of anesthetic induction time and increasing of sleeping time of Ketamin induced anesthesia, after IP injection of the Polar fraction extract of Humulus lupulus. The results obtained showed that the polar-fraction extract of Humulus lupulus has more sufficient sedative effects than diazepam and other under studied groups.
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Aims Studies have shown that probiotic bacteria inhibit the onset and progression of carcinogenesis through different pathways. Our objective in this study was to determine the effect of probiotic bacteria on the expression of... more
Aims Studies have shown that probiotic bacteria inhibit the onset and progression of carcinogenesis through different pathways. Our objective in this study was to determine the effect of probiotic bacteria on the expression of growth-related genes Rel A, IKB, and Casp3 in HT29 colon cancer cells Methods & Materials In this study, the Lactobacillus brevis probiotic bacteria were first cultured, and after the supply of media conditioning, they were treated on HT29 cancer cells. The bacterium’s cytotoxic effect (bacterial T cells) was investigated using a microculture tetrazolium test (MTT). DNA was extracted from the treated cells, and a DNA Ladder assay was performed. Also, the 4′,6-diamidino-2-phenylindole (DAPI) test was performed to show cell apoptosis. After ribonucleic acid (RNA) extraction and complementary DNA (cDNA) preparation to determine the mechanism of the effect of this bacterium on cellular signaling, the expression of growth-related genes Rel A, IKB, and Cas3 was meas...
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Abstract: In the present study, sedative and anti-anxiety drugs such as chemical drugs are used for the sedation and anti-anxiety but due to their side effects and economical issues, the significance of research on finding sedative and... more
Abstract: In the present study, sedative and anti-anxiety drugs such as chemical drugs are used for the sedation and anti-anxiety but due to their side effects and economical issues, the significance of research on finding sedative and anti-anxiety drugs with less side effects and their ability to substitute these synthetic drugs and substituting newer sedation and anti-anxiety compounds is obvious. Equisetum arvense is one of the plants that have the effect sedation and anti-anxiety. The aim of this research was to examine the effects of sedative, pre-anesthetic and anti-anxiety of Equisetum arvense with diazepam in rats. To different groups of male wistar rats received herbal extract Equisetum arvense with doses (100,200,400 mg/kg , IP) and Diazepam with dose of( 1/2 mg/kg , IP) , and Dimethyl sulfoxide with the equal volume. 30 minutes after assessing the relift/sleep inducing effect (induced sleep duration by ketamine 40 mg/kg, IP) anti-anxiety effects (using Elevated plus maze)...
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Background: Hepatitis B is the most common blood-borne viral infection that is considered as a major public health problem of the world's major health problems. The aim of this study was to investigate the prevalence of hepatitis B... more
Background: Hepatitis B is the most common blood-borne viral infection that is considered as a major public health problem of the world's major health problems. The aim of this study was to investigate the prevalence of hepatitis B virus (HBV) infection among blood donors referring to blood transfusion centers. Methods: This retrospective cross-sectional study was performed on 216004 volunteer blood donors referring to blood transfusion centers of Iran from the beginning of April 2011 to April 2015. Then the positive hepatitis B surface antigen (HBsAg) test was performed using the enzyme-linked immunosorbent assay (ELISA) and neutralization methods. Results: Of the 216004 blood donors, 279 (12.12%) were positive for HBsAg, and the incidence of infection was a decreasing trend over a four-year period. Among HbsAg positive cases, 97.14% and 2.86% were male and female, respectively. Significant differences between males and females were found (P=0.000). The number of HBsAg positive cases among married people (238 cases, 85.3%) in compared with single people (41 cases, 14.7%) was significantly higher (P=0.000). The average age of HBV infected cases was 39.6±10.3 years. Most HBsAg positive cases were 45-36 years old (30.8%) and lowest prevalence was seen in the age group above 56 years old (4.6%). Highest infected people with Hepatitis B Virus had low degree of education. Relationships between HBV infection with age and degree of education were statistically significant (P=0.000). Conclusion: The results of this study showed that based on our findings, prevalence of hepatitis B surface antigen among blood donors have declined significantly during the four years of study.
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The effects of TeucriumPoliumoil of medicinal plants on performance, carcass characteristics, and blood biochimecalof broiler chickens were studied in this experiment. During the experiment 400 chicken broilers were divided in four... more
The effects of TeucriumPoliumoil of medicinal plants on performance, carcass characteristics, and blood biochimecalof broiler chickens were studied in this experiment. During the experiment 400 chicken broilers were divided in four experimental groups with five repetitions: control group (Y1) without any herbal planet, group 2 (Y2) received 100 ppm of TeucriumPolium oil, group 3 (Y3)with 150ppm of TeucriumPolium oil, grop4 (Y4) received 200 ppm of TeucriumPolium oil.The addition of different levels ofTeucriumPoliumoil to the broiler diet had significant effect on performance (p 0.05).
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Background: Colon cancer is one of the most common cancers in the world. Probiotics are viable and useful microorganisms that have an effective role in controlling cancer by influencing the digestive enzymes of animals and humans,... more
Background: Colon cancer is one of the most common cancers in the world. Probiotics are viable and useful microorganisms that have an effective role in controlling cancer by influencing the digestive enzymes of animals and humans, inhibition of cancerous agents in the body and in vitro conditions, suppression of lotions, and cancer-inducing compounds and tumors in experimental animals. The present study was conducted to investigate the PTEN/ AKT1 cellular signaling pathway in adjacent cultures of Streptococcus thermophilus with HT29 colon cancer cells. Materials and Methods: Bacterial culture, supernatant and bacterial extract were prepared and the cells were treated with these materials. Cell necrosis was evaluated by the MTT method. Also, the expression of PTEN and AKT1 genes in HT29 cell line was investigated using the real-time PCR. Results: The results showed that the thermophiles bacterium reduced the expression of AKT1 genes, and increased the expression of PTEN and led the c...
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Antibiotic resistance is one of the common issues in the modern medicine so finding new agents with low side effects is necessary. Considering the biological active components exist in the sagebrush and its usage in the traditional... more
Antibiotic resistance is one of the common issues in the modern medicine so finding new agents with low side effects is necessary. Considering the biological active components exist in the sagebrush and its usage in the traditional medicine, it seems that, it may have significant antibacterial activity. The objective of present study was to evaluate antibacterial activity of methanolic extract and essence of sagebrush against four strain including Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. In this study, the effect of methanolic extract of sagebrush was tested against above mention ed strains after achieving by Soxhlet method. The concentrations of 20, 30, 50 and 400 mg/ml of plant were prepared using dimethyl sulfoxide then its antimicrobial activity was evaluated using well distribution and tube dilution. Data were analysed by ANOVA and chi-square at the level P<0.01. Results showed that methanolic extract of sagebrush prevents of growt...
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Introduction: Colon cancer has spread to developed countries. Probiotic bacteria can play an important role in reducing cancer and treating colon cancer. The aim of this study was to determine the effect of adjacent bovine Lactobacillus... more
Introduction: Colon cancer has spread to developed countries. Probiotic bacteria can play an important role in reducing cancer and treating colon cancer. The aim of this study was to determine the effect of adjacent bovine Lactobacillus rhamnose culture on the expression of casp3 and bax genes in cancer cell. Materials & Methods: In this experimental study after bacterial culture, supernatant and bacterial extract were prepared and the cells were treated with these materials. The effects of cell cytotoxicity of the cell line on the HT29 cell line were investigated using MTT method. After extraction of RNA and preparation of cDNA, the expression of bax, casp3 genes in the HT29 cell line was investigated using Real Time PCR. Findings: The results of MTT assay showed that Lactobacillus ramnose at a concentration of 10 μg/ml reduced the survival of HT-29 cells by 51.49±8.32%, and the coloring results detection showed that treatment of HT29 cells with Lactobacillus rhamnosis caused quali...
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In the present study, sedative and anti-anxiety drugs such as chemical drugs are used for the sedation and anti-anxiety but due to their side effects and economical issues, the significance of research on finding sedative and anti-anxiety... more
In the present study, sedative and anti-anxiety drugs such as chemical drugs are used for the sedation and anti-anxiety but due to their side effects and economical issues, the significance of research on finding sedative and anti-anxiety drugs with less side effects and their ability to substitute these synthetic drugs and substituting newer sedation and anti-anxiety compounds is obvious. Equisetum arvense is one of the plants that have the effect sedation and anti-anxiety. The aim of this research was to examine the effects of sedative, pre-anesthetic and anti-anxiety of Equisetum arvense with diazepam in rats. To different groups of male wistar rats received herbal extract Equisetum arvense with doses (100,200,400 mg/kg , IP) and Diazepam with dose of( 1/2 mg/kg , IP) , and Dimethyl sulfoxide with the equal volume. 30 minutes after assessing the relift/sleep inducing effect (induced sleep duration by ketamine 40 mg/kg, IP) anti-anxiety effects (using Elevated plus maze). The resu...
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Cervical cancer is the fourth most common cancer and the second cause of cancer-related death among women. Over the past two decades, green tea catechins and curcumin have received much attention for their role in preventing... more
Cervical cancer is the fourth most common cancer and the second cause of cancer-related death among women. Over the past two decades, green tea catechins and curcumin have received much attention for their role in preventing carcinogenesis. In this study, we evaluated the effects of the catechin metabolites and curcumin on cervical cancer cell proliferation and apoptosis. For this aim, the Ca Ski cell line was treated with different doses of catechin metabolites and curcumin. MTT assay and Flow cytometry were employed to investigate the cytotoxic effects of catechin metabolites and curcumin on the Ca Ski cell line. Real-time PCR and western blot were performed to evaluate the VEGF expression. Also, Real-Time PCR was performed to determine the expression level of microRNAs. Results showed that catechin metabolites along with curcumin reduce the VEGF expression. Further, miR-210 and miR-21 as oncogenic microRNAs were down-regulated, while it was reverse for miR-126 as a tumor-suppressor microRNA. Besides, MTT and Flow cytometry results showed that after using catechin metabolites with curcumin, cell survival was reduced by inducing apoptosis. In conclusion, catechin metabolites produced by intestinal microbiota besides the curcumin could serve as a promising therapeutic approach for women with cervical cancer.
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Background Generally, hepatitis C has been identified as one of the major health issues that about 3% of the world’s population have been threatened and affected by it (about 170 million people), and also, it can be considered a factor in... more
Background Generally, hepatitis C has been identified as one of the major health issues that about 3% of the world’s population have been threatened and affected by it (about 170 million people), and also, it can be considered a factor in acute and chronic hepatitis. Methods The aim of this study is to determine the prevalence of HCV genotypes in Azerbaijan patients. In this study, sampling was done on the referred patients to the hospitals (Mahallati and Behbud Hospital). RNA was extracted after isolation of plasma, and then, after the synthesizing of cDNA, the sample was carried out to the laboratory for performing the real-time PCR in order to determine the genotypes. Results The evaluation of HCV genotypes in positive plasma samples showed that dominant subsets were remarkable and the mean age of the patients was 37/3 ± 11/8 (in the age range of 2–63). Among the 235 patients,139 of them (59%) were male. Statistically, the average number of women was more than men ( T test, P < 0/05). 1b genotype was reported 70% in the patients above 40 years old, and also, it was reported as 71/6% in the patients under 40 years old that was not statistically significant. The incidence of serotype 3a was higher among the patients younger than 40 years old (3a was 18.1% vs. 15%), and this serotype was prevalent among men (3a was 18.7% vs. 14.6%), which was statistically significant. Conclusion The findings indicate that among Azerbaijan’s patients with chronic hepatitis C, genotypes 1b (71.1%) and 3a (17%) were dominant.
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Background: Pseudomonas aeruginosa with widely distributed in nature, for human beings is considered an opportunistic pathogen that causes infections of broad-spectrum, including administrative, respiratory, septicemia and bacteremia and... more
Background: Pseudomonas aeruginosa with widely distributed in nature, for human beings is considered an opportunistic pathogen that causes infections of broad-spectrum, including administrative, respiratory, septicemia and bacteremia and sepsis in patients with the burning city of Ahvaz. Method and Material: A total of 95isolates of Pseudomonas aeruginosa isolated from burn patients from January 2015 assemble and biochemical identification test, then they are antibiotic resistance in E. test and disk diffusion method were compared. Findings: From 95 different clinical isolates of Pseudomonas aeruginosa isolated from E. test with the highest sensitivity to the antibiotic ceftazidime , 70(68/73%) and ciprofloxacin 50 (63/52%) and gentamicin 48 (52 /50 %) and the antibiotic imipenem 44 (31/46%) were sensitive and disk diffusion method antibiotic ceftazidime, 67(52/70%) and the antibiotic Ciprofloxacin 51(68/53%) and safety antibiotic imipenem 49(57/51%) and gentamicin 48(52/50%) were s...
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Aims: Defensins are cysteine-rich antimicrobial cationic peptides and BAX is a proapoptotic gene that can cause cell death. This study aimed to investigate the effect of cellular extract of co-cultured Lactobacillus casei on the... more
Aims: Defensins are cysteine-rich antimicrobial cationic peptides and BAX is a proapoptotic gene that can cause cell death. This study aimed to investigate the effect of cellular extract of co-cultured Lactobacillus casei on the expression of BAX and human β-defensin 2 (hBD-2) genes in HT29 cells. Methods & Materials: This experimental study was conducted in the Research Center for Pharmaceutical Nanotechnology of Tabriz University of Medical Sciences in 2017. The HT29 cell line was obtained from the Pasteur Institute of Iran, and cells were assessed using Microculture Tetrazolium Test (MTT) after culturing. DNA was extracted from the treated cells, and then the DNA ladder assay was carried out. After preparing cDNA, the expression levels of BAX and hBD-2 genes in the HT29 cell line were measured using a real-time Polymerase Chain Reaction (PCR) method. Findings: The results of the MTT assay indicated that Lactobacillus casei inhibited the proliferation of HT29 cells and induced apo...
Background and Objective: The emergence of drug resistance in Mycobacterium tuberculosis and especially drug resistant strains has created problems for the treatment of tuberculosis control, worldwide. This study was performed to evaluate... more
Background and Objective: The emergence of drug resistance in Mycobacterium tuberculosis and especially drug resistant strains has created problems for the treatment of tuberculosis control, worldwide. This study was performed to evaluate the changes in N-acetyltransferase (NAT) pattern of methylsonone in patients who did not respond to first-line treatment for Mycobacterium tuberculosis.
Methods: This descriptive-analytical study was performed on 200 patients with tuberculosis. After preparing the blood sample, saturated salt method was used to extract DNA. For the determination of quantity and quality of DNA, two methods of spectrophotometry and electrophoresis on agarose gel were used. The methylation pattern of NAT1 gene was investigated by HRM method.
Results: 34 patients were resistant to the first line of treatment. 18 patients showed hypermethylation pattern, 12 patients showed non-methylated pattern and 4 patients showed hypomethylated pattern. 166 patients were not resistant to the first line, of which 23 patients in hypermethylated group, 120 patients in group Non-methylated and 23 patients were in the hypomethylated row. There was a statistically significant relationship between methylation level and drug resistance (P<0.05).
Conclusion: This study showed that resistance to first-line treatment of Mycobacterium tuberculosis was related to the level of N-acetyltransferase gene methylation.
Keywords: Tuberculosis, Mycobacterium Tuberculosis, Methylation, N-acetyltransferase, Drug Resistance
Methods: This descriptive-analytical study was performed on 200 patients with tuberculosis. After preparing the blood sample, saturated salt method was used to extract DNA. For the determination of quantity and quality of DNA, two methods of spectrophotometry and electrophoresis on agarose gel were used. The methylation pattern of NAT1 gene was investigated by HRM method.
Results: 34 patients were resistant to the first line of treatment. 18 patients showed hypermethylation pattern, 12 patients showed non-methylated pattern and 4 patients showed hypomethylated pattern. 166 patients were not resistant to the first line, of which 23 patients in hypermethylated group, 120 patients in group Non-methylated and 23 patients were in the hypomethylated row. There was a statistically significant relationship between methylation level and drug resistance (P<0.05).
Conclusion: This study showed that resistance to first-line treatment of Mycobacterium tuberculosis was related to the level of N-acetyltransferase gene methylation.
Keywords: Tuberculosis, Mycobacterium Tuberculosis, Methylation, N-acetyltransferase, Drug Resistance