Novel partially degradable waterborne pressure sensitive adhesives can be easily removed from substrates under mild alkali conditions opening the door for sustainable (less solvent and energy consumption) recycling processes.
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Background Glioblastoma (GBM) is the most common malignant primary brain tumour in adults. Circulating biomarkers may assist in the processes of differential diagnosis and response assessment. GBM cells release extracellular vesicles... more
Background Glioblastoma (GBM) is the most common malignant primary brain tumour in adults. Circulating biomarkers may assist in the processes of differential diagnosis and response assessment. GBM cells release extracellular vesicles containing a subset of proteins and nucleic acids. We previously demonstrated that exosomes isolated from the serum of GBM patients had an increased expression of RNU6-1 compared to healthy subjects. In this exploratory study we investigated the role of this small non-coding RNA as a diagnostic biomarker for GBM versus other brain lesions with some potential radiological similarities. Methods We analysed the expression of RNU6-1 in circulating exosomes of GBM patients (n=18), healthy controls (n=30), and patients with subacute stroke (n=30), acute/subacute haemorrhage (n=30), acute demyelinating lesions (n=18), brain metastases (n=21) and primary central nervous system lymphoma (PCNSL; n=12) using ddPCR. Results Expression of RNU6-1 was significantly hi...
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... Amaia Agirre, Julia Nase, Elise Degrandi, Costantino Creton ... This may be due to extensive primary cyclization (Scheme 1) which is more pronounced in the case of short cross-linkers.(28-30) Bouvier-Fontes et al.(31) reported that... more
... Amaia Agirre, Julia Nase, Elise Degrandi, Costantino Creton ... This may be due to extensive primary cyclization (Scheme 1) which is more pronounced in the case of short cross-linkers.(28-30) Bouvier-Fontes et al.(31) reported that primary cyclization was the cause for which ...
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Poly(n-butyl acrylate) (polyn-BA) polymers synthesized by radical bulk polymerization in the presence and absence of a high concentration (0.4 mol L−1) of carbon tetrabromide (CBr4) as a chain transfer agent at nominal temperatures of 60,... more
Poly(n-butyl acrylate) (polyn-BA) polymers synthesized by radical bulk polymerization in the presence and absence of a high concentration (0.4 mol L−1) of carbon tetrabromide (CBr4) as a chain transfer agent at nominal temperatures of 60, 100 and 140 °C were fully characterized by 1D and 2D NMR, SEC/MALS and MALDI-TOF mass spectrometry. The structures generated by chain transfer to CBr4 in secondary chain-end radicals and reinitiation of polymer chains by CBr3 radicals formed by chain transfer to CBr4 reactions were identified by MALDI-TOF and NMR analysis. The potential structures that might have been created by chain-transfer to tertiary radicals (quaternary carbons with a Br unit) formed by backbiting or intermolecular chain transfer to polymer could not be detected and hence their abundance was not important. The branching density (BD) of the polymers synthesized in the presence and absence of CBr4 was also determined. The BD increases with temperature in both cases, and for eac...
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ABSTRACT The molar mass and the radius of gyration of three poly N-vinyl formamide (polyNVF) synthesized in aqueous solution polymerization were characterized using two different fractionation techniques: size exclusion chromatography... more
ABSTRACT The molar mass and the radius of gyration of three poly N-vinyl formamide (polyNVF) synthesized in aqueous solution polymerization were characterized using two different fractionation techniques: size exclusion chromatography (SEC) and asymmetric-flow field-flow fractionation (AF4) coupled with a multiangle light scattering (MALS) and a refractive index (RI) detector. For the sake of comparison, the polymers were also characterized by MALS using the Zimm plot approach (no fractionation). The dn dc−1 of the poly (N-vinyl formamide) was measured (0.1564 mL g−1) and it was found to be insensitive to the molar mass (in the range 150-450 kDa) and also to the eluents used (DDI water or mixed eluent DDI water/acetonitrile (80 : 20) at pH = 5.5). Interestingly, the concentrations of the samples injected in the SEC and AF4 should be different because concentrations in the range of 20-40 mg mL−1 used for the AF4 caused overloading and anomalous elution in the SEC and hence misleading molar masses. At adequate concentrations in each fractionation equipment, the molar masses were in reasonable good agreement although AF4/MALS provided larger values than the other two techniques likely because samples were not filtered before injection. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42434.
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RAFT polymerization has emerged as one of the most versatile reversible deactivation radical polymerization techniques and is capable of polymerizing a wide range of monomers under various conditions.
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ABSTRACT The architecture of crosslinked acrylic latexes of 2-ethylhexyl acrylate, methacrylic acid, and a crosslinker agent are investigated. These polymers contain a significant amount of gel content. Branching is obtained by liquid-... more
ABSTRACT The architecture of crosslinked acrylic latexes of 2-ethylhexyl acrylate, methacrylic acid, and a crosslinker agent are investigated. These polymers contain a significant amount of gel content. Branching is obtained by liquid- and melt-state 13C NMR spectroscopy. For the first time, the branching densities (long and short chains) of the soluble and gel fractions are measured independently. Branching is higher for the polymers produced with the most-efficient crosslinkers, namely those which yield a higher crosslinking density. The branching (long chains) and the molecular-weight distribution (MWD) of the soluble fraction are characterized by size-exclusion chromatography (SEC) coupled with triple detection. Coelution of branched chains at large elution volumes is observed in the SEC chromatograms indicating the highly branched nature of the sol fractions of the latexes.
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Purpose. Glioblastomas (GBMs) are the most frequent malignant brain tumors in adults, and their prognosis remains dismal despite intensive research and therapeutic advances. Diagnostic biomarkers would be clinically meaningful to allow... more
Purpose. Glioblastomas (GBMs) are the most frequent malignant brain tumors in adults, and their prognosis remains dismal despite intensive research and therapeutic advances. Diagnostic biomarkers would be clinically meaningful to allow for early detection of the tumor and for those cases in which surgery is contraindicated or biopsies result inconclusive. Recent findings show that GBM cells release microvesicles that contain a select subset of cellular proteins and RNA. We undertook this study to assess whether we could find a miRNA signature in microvesicles isolated from serum of GBM patients that could serve as a diagnostic tool. Patients and Methods. To control disease heterogeneity we used patients with newly diagnosed GBM. In the discovery stage, TLDA PCR based-arrays followed by individual quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) were used to test the differences in the miRNAs expression level of serum microvesicles between 25 GBM patients and 25...
ABSTRACT Capillary hydrodynamic fractionation (CHDF) with turbidity detection at a single wavelength is an analytical technique that is often used for sizing the sub-micrometric particles of hydrophobic colloids. This article investigates... more
ABSTRACT Capillary hydrodynamic fractionation (CHDF) with turbidity detection at a single wavelength is an analytical technique that is often used for sizing the sub-micrometric particles of hydrophobic colloids. This article investigates three sources of errors that affect the particle size distribution (PSD) estimated by CHDF: diameter calibration errors, uncertainties in the particle refractive index (PRI), and instrumental broadening (IB). The study is based on simulated and experimental examples that involve unimodal and bimodal PSDs. Small errors in the diameter calibration curve can produce important deviations in the number average diameter due to systematic shifts suffered by the PSD modes. Moderate uncertainties in the PRI are unimportant in the analysis of unimodal PSDs, but in the specific case of bimodal PSDs, errors in the PRI can strongly affect the estimated number concentration of each mode. The typical IB correction (based on the IB function estimated from narrow standards) produces slightly erroneous average diameters but can lead to PSDs with underestimated widths and distorted shapes. In practice, the three investigated sources of errors can be present simultaneously, and uncertainties in the average diameters, the shape and width of the PSD, and the number concentration of the PSD modes are unavoidable. (C) 2014 Published by Elsevier B.V. on behalf of Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences.
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ABSTRACT The wide application of polymers makes continuous demand for novel polymeric materials with tailored properties. The characterization of polymers becomes very important task within the process of creation of novel polymeric... more
ABSTRACT The wide application of polymers makes continuous demand for novel polymeric materials with tailored properties. The characterization of polymers becomes very important task within the process of creation of novel polymeric materials. In this article, the principles and scope of the basic characterization techniques are presented for the determination of the important polymer properties: molecular structure, size, and organization. The spectroscopic techniques (UV, IR, Raman, and NMR) have been discussed in relation to the determination of molecular structure, cross-linking, and branching density determination. In this regard, matrix-assisted laser desorption ionization–time-of-flight mass spectrometry is discussed as well. Fundamentals of each of them are presented shortly, supported by suitable examples. The application of chromatographic techniques, such as size exclusion chromatography and AsFFF, is discussed in a more detailed way and in relation to molecular size determination.
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Research Interests: Adolescent, Neuro-Oncology, Humans, Glioblastoma, Female, and 15 moreMale, Young Adult, Follow-up studies, Exosomes, Aged, Middle Aged, microRNAs, Adult, Prognosis, Neurosciences, Case Control Studies, Cohort Studies, real time polymerase chain reaction, reverse transcriptase polymerase chain reaction, and Neoplasm staging
... Amaia Agirre, Julia Nase, Elise Degrandi, Costantino Creton ... This may be due to extensive primary cyclization (Scheme 1) which is more pronounced in the case of short cross-linkers.(28-30) Bouvier-Fontes et al.(31) reported that... more
... Amaia Agirre, Julia Nase, Elise Degrandi, Costantino Creton ... This may be due to extensive primary cyclization (Scheme 1) which is more pronounced in the case of short cross-linkers.(28-30) Bouvier-Fontes et al.(31) reported that primary cyclization was the cause for which ...
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... Figure 14. The effect of the SA content on the shear resistance obtained by the holding power measurement at 30 8C over different substrates. ... 2006, 82, 267310. [17] M. Aouinti, P. Bertrand, F. Poncin-Epaillard, Plas-mas Polym.... more
... Figure 14. The effect of the SA content on the shear resistance obtained by the holding power measurement at 30 8C over different substrates. ... 2006, 82, 267310. [17] M. Aouinti, P. Bertrand, F. Poncin-Epaillard, Plas-mas Polym. 2003, 8, 225236. [18] JM Asua, Prog. Polym. ...