Yasser Osman
Phone: +2-01001266350
Address: 1 Mothaf el-matarya st. - Cario - Egypt
24 A Mostafa el-maraghy st. - Helwan - Cairo - Egypt
Address: 1 Mothaf el-matarya st. - Cario - Egypt
24 A Mostafa el-maraghy st. - Helwan - Cairo - Egypt
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Papers by Yasser Osman
for secondary metabolites, thin layer chromatography (TLC), Fourier Transform Infrared spectroscopy (FTIR), and Gas
Chromatography Mass spectroscopy (GCMS). Lawsonia inermis L. (Lythraceae) commonly called henna known for its
cosmetic properties. The active ingredients in the leaves were extracted and fractionated using chloroform, ether, methanol,
and ethyl-acetate. The resultant fractions primarily analyzed for secondary metabolites and secondarily. The phytochemical
screening revealed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, quinines, resins and sterols. The
presence or absence of all the phytochemicals in these solvents was more or less similar between both samples. TLC
profiling shows 9 bands of chemical compounds that are significantly similar in both samples. The FTIR spectrum
confirmed the presence of 8 functional in both samples and an additional of aliphaticiodo compound in Egyptian sample.
Sixteen and seventeen compounds identified in Nigerian and Egyptian samples respectively by GCMS analysis. Lawsone
(2-hydroxy 1, 2-naphtoquinone) is one out of 7 common chemical compounds identified in both samples. The common
compound identified by TLC, FTIR, and GCMS were found to be significantly similar in both quantity and quality. Thus,
the research confirmed henna to be an important source of phytochemicals of immense pharmaceutical significance.
antifungal activity of Lawsonia inermis L. In vitro study was carried out using agar dilution method. In a total of 50 clinical
samples, 4 different species were identified namely; Microsporum canis, Trichophyton tonsurans, Trichophyton rubrum, and
Trichophyton mentagrophytes. However, the results showed that hexane extract exerted a strong antifungal activity against all the
identified etiological agents, with a minimum inhibitory concentration of 625ìg/ml except Microsporum canis which resisted the
minimum concentration but susceptible to the higher concentrations. The in vivo study was carried out using 15 naturally infected
goats. First, second and third group of animals were treated with henna paste, aqueous and ethanolic extract respectively, fourth
group were treated with clotrimazole as a positive control while negative control (fifth) group were left untreated with neither
henna nor clotrimazole. The treatments were compared and the results showed that henna paste had the highest efficacy against
all the types of ringworm tested compared to the remaining treatments. Disappearance of lesion and complete repair of the hair
was observed at 30 days after treatment. Significantly similar result was observed in the group of animals treated with aqueous
extract, ethanol extract, and clotrimazole in all the parameters. Significant different was only observed between groups treated
and negative control.
for secondary metabolites, thin layer chromatography (TLC), Fourier Transform Infrared spectroscopy (FTIR), and Gas
Chromatography Mass spectroscopy (GCMS). Lawsonia inermis L. (Lythraceae) commonly called henna known for its
cosmetic properties. The active ingredients in the leaves were extracted and fractionated using chloroform, ether, methanol,
and ethyl-acetate. The resultant fractions primarily analyzed for secondary metabolites and secondarily. The phytochemical
screening revealed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, quinines, resins and sterols. The
presence or absence of all the phytochemicals in these solvents was more or less similar between both samples. TLC
profiling shows 9 bands of chemical compounds that are significantly similar in both samples. The FTIR spectrum
confirmed the presence of 8 functional in both samples and an additional of aliphaticiodo compound in Egyptian sample.
Sixteen and seventeen compounds identified in Nigerian and Egyptian samples respectively by GCMS analysis. Lawsone
(2-hydroxy 1, 2-naphtoquinone) is one out of 7 common chemical compounds identified in both samples. The common
compound identified by TLC, FTIR, and GCMS were found to be significantly similar in both quantity and quality. Thus,
the research confirmed henna to be an important source of phytochemicals of immense pharmaceutical significance.
antifungal activity of Lawsonia inermis L. In vitro study was carried out using agar dilution method. In a total of 50 clinical
samples, 4 different species were identified namely; Microsporum canis, Trichophyton tonsurans, Trichophyton rubrum, and
Trichophyton mentagrophytes. However, the results showed that hexane extract exerted a strong antifungal activity against all the
identified etiological agents, with a minimum inhibitory concentration of 625ìg/ml except Microsporum canis which resisted the
minimum concentration but susceptible to the higher concentrations. The in vivo study was carried out using 15 naturally infected
goats. First, second and third group of animals were treated with henna paste, aqueous and ethanolic extract respectively, fourth
group were treated with clotrimazole as a positive control while negative control (fifth) group were left untreated with neither
henna nor clotrimazole. The treatments were compared and the results showed that henna paste had the highest efficacy against
all the types of ringworm tested compared to the remaining treatments. Disappearance of lesion and complete repair of the hair
was observed at 30 days after treatment. Significantly similar result was observed in the group of animals treated with aqueous
extract, ethanol extract, and clotrimazole in all the parameters. Significant different was only observed between groups treated
and negative control.