This paper reports a digital polymerase chain reaction platform with liquid beads, liquid droplet... more This paper reports a digital polymerase chain reaction platform with liquid beads, liquid droplets encapsulated in a hard shell.
"This report has been prepared as part of the National Demonstration, Education and Engageme... more "This report has been prepared as part of the National Demonstration, Education and Engagement Program (NDEEP)"-p. 2.
Sunlight disinfection is important for treatment of wastewater within maturation ponds. This stud... more Sunlight disinfection is important for treatment of wastewater within maturation ponds. This study analyses the movement of Escherichi coli within a slice of a maturation pond, being affected by stratification, sunlight attenuation and mixing driven by wind shear and natural convection using computational fluid dynamics (CFD). Since the exposure to ultraviolet light is most effective in the near-surface region of the pond, natural convective mixing mechanisms to transport the pathogens from the lower parts of the pond are critical for disinfection efficacy. Different turbulence models are considered for closure of the momentum conservation equations and compared with a laminar flow simulation and a completely stirred tank reactor (CSTR) model. The effect of turbulence and stratification is shown to be significant for thermal and velocity distributions, and predictions of E. coli die-off. Greater volume-averaged E. coli die-off was predicted by the computationally convenient CSTR model than the CFD turbulence and laminar models. The simulation results are compared with experimental data and show that complete vertical mixing occurs in a diurnal pattern aiding die-off in sunlight-attenuating water. Practical applications of the model can assist in management strategies for maturation ponds such as off-take locations/times and evaluating seasonal variations in sunlight disinfection.
The Hinze Dam is located in the Gold Coast hinterland and is the primary source of water supply f... more The Hinze Dam is located in the Gold Coast hinterland and is the primary source of water supply for the Gold Coast region. Sporadic and unpredictable taste and odour events caused by geosmin and 2-methylisoborneol (MIB) are an ongoing problem in the Hinze Dam. To investigate potential ecological and physiological triggers of these events, a 12-month surface-sampling regime was undertaken. Concentrations of geosmin, MIB, nitrogen and phosphorus were measured. Algal and cyanobacterial counts were performed. Water temperature, rainfall and dam capacity were also recorded. The occurrence of geosmin was found to correlate significantly with Anabaena numbers, water temperature and dam capacity. The occurrence of MIB correlated with increasing ammonia. No significant correlations were observed with the other nutrients or physical parameters measured. Overall, this study demonstrated that high concentrations of geosmin detected in dam surface waters was strongly correlated with an increase ...
Five isolates of a filamentous bacterial morphotype with the distinctive diagnostic microscopic f... more Five isolates of a filamentous bacterial morphotype with the distinctive diagnostic microscopic features of Eikelboom Type 1863 were obtained from activated sludge sewage treatment plants in Victoria, Australia. On the basis of phenotypic evidence and 16S rDNA sequence data, these isolates proved to be polyphyletic. Two (Ben 06 and Ben 06C) are from the Chryseobacterium subgroup which is in the Cytophaga group, subdivision I of the Flexibacter-Cytophaga-Bacteroides phylum. Two (Ben 56 and Ben 59) belong to the genus Acinetobacter, and one (Ben 58) is a Moraxella sp., closest to Mor. osloensis. The significance of these findings to the reliance on microscopic features for identification of these filamentous bacteria in activated sludge is discussed.
This paper reports a digital polymerase chain reaction platform with liquid beads, liquid droplet... more This paper reports a digital polymerase chain reaction platform with liquid beads, liquid droplets encapsulated in a hard shell.
"This report has been prepared as part of the National Demonstration, Education and Engageme... more "This report has been prepared as part of the National Demonstration, Education and Engagement Program (NDEEP)"-p. 2.
Sunlight disinfection is important for treatment of wastewater within maturation ponds. This stud... more Sunlight disinfection is important for treatment of wastewater within maturation ponds. This study analyses the movement of Escherichi coli within a slice of a maturation pond, being affected by stratification, sunlight attenuation and mixing driven by wind shear and natural convection using computational fluid dynamics (CFD). Since the exposure to ultraviolet light is most effective in the near-surface region of the pond, natural convective mixing mechanisms to transport the pathogens from the lower parts of the pond are critical for disinfection efficacy. Different turbulence models are considered for closure of the momentum conservation equations and compared with a laminar flow simulation and a completely stirred tank reactor (CSTR) model. The effect of turbulence and stratification is shown to be significant for thermal and velocity distributions, and predictions of E. coli die-off. Greater volume-averaged E. coli die-off was predicted by the computationally convenient CSTR model than the CFD turbulence and laminar models. The simulation results are compared with experimental data and show that complete vertical mixing occurs in a diurnal pattern aiding die-off in sunlight-attenuating water. Practical applications of the model can assist in management strategies for maturation ponds such as off-take locations/times and evaluating seasonal variations in sunlight disinfection.
The Hinze Dam is located in the Gold Coast hinterland and is the primary source of water supply f... more The Hinze Dam is located in the Gold Coast hinterland and is the primary source of water supply for the Gold Coast region. Sporadic and unpredictable taste and odour events caused by geosmin and 2-methylisoborneol (MIB) are an ongoing problem in the Hinze Dam. To investigate potential ecological and physiological triggers of these events, a 12-month surface-sampling regime was undertaken. Concentrations of geosmin, MIB, nitrogen and phosphorus were measured. Algal and cyanobacterial counts were performed. Water temperature, rainfall and dam capacity were also recorded. The occurrence of geosmin was found to correlate significantly with Anabaena numbers, water temperature and dam capacity. The occurrence of MIB correlated with increasing ammonia. No significant correlations were observed with the other nutrients or physical parameters measured. Overall, this study demonstrated that high concentrations of geosmin detected in dam surface waters was strongly correlated with an increase ...
Five isolates of a filamentous bacterial morphotype with the distinctive diagnostic microscopic f... more Five isolates of a filamentous bacterial morphotype with the distinctive diagnostic microscopic features of Eikelboom Type 1863 were obtained from activated sludge sewage treatment plants in Victoria, Australia. On the basis of phenotypic evidence and 16S rDNA sequence data, these isolates proved to be polyphyletic. Two (Ben 06 and Ben 06C) are from the Chryseobacterium subgroup which is in the Cytophaga group, subdivision I of the Flexibacter-Cytophaga-Bacteroides phylum. Two (Ben 56 and Ben 59) belong to the genus Acinetobacter, and one (Ben 58) is a Moraxella sp., closest to Mor. osloensis. The significance of these findings to the reliance on microscopic features for identification of these filamentous bacteria in activated sludge is discussed.
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