[go: up one dir, main page]
Wikipedia

Jennifer Doudna

Jennifer Anne Doudna ForMemRS (/ˈdaʊdnə/;[1] born February 19, 1964)[2] is an American biochemist who has pioneered work in CRISPR gene editing, and made other fundamental contributions in biochemistry and genetics. She received the 2020 Nobel Prize in Chemistry, with Emmanuelle Charpentier, "for the development of a method for genome editing."[3][4] She is the Li Ka Shing Chancellor's Chair Professor in the department of chemistry and the department of molecular and cell biology at the University of California, Berkeley. She has been an investigator with the Howard Hughes Medical Institute since 1997.[5]

Jennifer Doudna
ForMemRS
Doudna in 2023
Born
Jennifer Anne Doudna

(1964-02-19) February 19, 1964 (age 60)
Washington, D.C., U.S.
Education
Known for
Spouse
Jamie Cate
​
(m. 2000)​
Awards
Scientific career
Fields
Institutions
ThesisTowards the Design of an RNA Replicase (1989)
Doctoral advisorJack Szostak
Other academic advisorsThomas Cech
Doctoral students
WebsiteDoudna Lab website
Hughes Institute website

In 2012, Doudna and Emmanuelle Charpentier were the first to propose that CRISPR-Cas9 (enzymes from bacteria that control microbial immunity) could be used for programmable editing of genomes,[6][7] which has been called one of the most significant discoveries in the history of biology.[8] Since then, Doudna has been a leading figure in what is referred to as the "CRISPR revolution" for her fundamental work and leadership in developing CRISPR-mediated genome editing.[6]

Doudna at the Innovative Genomics Institute

Doudna's awards and fellowships include the 2000 Alan T. Waterman Award for her research on the structure of a ribozyme, as determined by X-ray crystallography[9] and the 2015 Breakthrough Prize in Life Sciences for CRISPR-Cas9 genome editing technology, with Charpentier.[10] She has been a co-recipient of the Gruber Prize in Genetics (2015),[11] the Tang Prize (2016),[12] the Canada Gairdner International Award (2016),[13] and the Japan Prize (2017).[14] She was named one of the Time 100 most influential people in 2015,[15] and in 2023 was inducted into the National Inventors Hall of Fame.[16]

Early life and education

edit

Jennifer Doudna was born February 19, 1964, in Washington, D.C., as the daughter of Dorothy Jane (Williams) and Martin Kirk Doudna.[2][17] Her father received his PhD in English literature from the University of Michigan, and her mother held a master's degree in education.[6][7] When Doudna was seven years old, the family moved to Hawaii so her father could accept a teaching position in American literature at the University of Hawaii at Hilo.[6][7] Doudna's mother earned a second master's degree in Asian history from the university and taught history at a local community college.[6][7]

Growing up in Hilo, Hawaii, Doudna was fascinated by the environmental beauty of the island and its flora and fauna. Nature built her sense of curiosity and her desire to understand the underlying biological mechanisms of life.[6][7] This was coupled with the atmosphere of intellectual pursuit that her parents encouraged at home. Her father enjoyed reading about science and filled the home with many books on popular science.[6][7] When Doudna was in the sixth grade, he gave her a copy of James Watson's 1968 book on the discovery of the structure of DNA, The Double Helix, which was a major inspiration.[18] Doudna also developed her interest in science and mathematics in school.[6][18] Even though Doudna was told that "Women don't go into science," she knew that she wanted to be a scientist no matter what. Nothing said to her made her doubt it, Doudna said, "When someone tells me I can't do something and I know that I can, it just makes me more resolved to do it."[19]

While she attended Hilo High School, Doudna's interest in science was nurtured by her 10th-grade chemistry teacher, Ms. Jeanette Wong, whom she has routinely cited as a significant influence in sparking her nascent scientific curiosity.[18][20][21][22] A visiting lecturer on cancer cells further encouraged her pursuit of science as a career choice.[18] She spent a summer working in the University of Hawaii at Hilo lab of noted mycologist Don Hemmes and graduated from Hilo High School in 1981.[23]

Doudna was an undergraduate student at Pomona College in Claremont, California, where she studied biochemistry.[6][7] During her freshman year, while taking a course in general chemistry, she questioned her own ability to pursue a career in science, and considered switching her major to French as a sophomore.[24][6] However, her French teacher suggested she stick with science.[6][24] Chemistry professors Fred Grieman and Corwin Hansch at Pomona had a major impact on her.[24] She started her first scientific research in the lab of professor Sharon Panasenko.[24] She earned her Bachelor of Arts degree in biochemistry in 1985.[24][25] She chose Harvard Medical School for her doctoral study and earned a PhD in Biological Chemistry and Molecular Pharmacology in 1989.[5][26] Her Ph.D. dissertation was on a system that increased the efficiency of a self-replicating catalytic RNA[10] and was supervised by Jack W. Szostak.[10]

Career and research

edit

After her PhD, she held research fellowships in molecular biology at the Massachusetts General Hospital and in genetics at Harvard Medical School.[5] From 1991 to 1994, she was Lucille P. Markey Postdoctoral Scholar in Biomedical Science at the University of Colorado Boulder, where she worked with Thomas Cech.[5] As of 2022, Doudna has an h-index of 141 according to Google Scholar[27] and of 111 according to Scopus.[28]

Research on ribozyme structure and function

edit

Early in her scientific career, Doudna worked to uncover the structure and biological function of RNA enzymes or ribozymes.[24] While in the Szostak lab, Doudna re-engineered the self-splicing Tetrahymena Group I catalytic intron into a true catalytic ribozyme that copied RNA templates.[24] Her focus was on engineering ribozymes and understanding their underlying mechanisms; however, she came to realize that not being able to see the molecular mechanisms of ribozymes was a major problem. Doudna went to the lab of Thomas Cech at the University of Colorado Boulder to crystallize and determine the three-dimensional structure of a ribozyme for the first time, so ribozyme structure could be compared with that of Enzymes, the catalytic Proteins.[24] She started this project at the Cech lab in 1991 and finished it at Yale University in 1996.[10] Doudna joined Yale's Department of Molecular Biophysics and Biochemistry as an assistant professor in 1994.[5]

 
Portrait of Jennifer Doudna by Christopher Michel for the National Academies

X-ray diffraction-based structure of active site of a ribozyme at Yale

edit
 
Shape of a self-splicing intron with two exons (shown in red and blue). DS Goodsell, 2005, PDB

At Yale, Doudna's group was able to crystallize and solve the three-dimensional structure of the catalytic core of the Tetrahymena Group I ribozyme.[24] They showed that a core of five magnesium ions clustered in one region of the P4-P6 domain of the ribozyme, forming a hydrophobic core around which the rest of the structure could fold.[24] This is analogous but chemically distinct from, the way proteins typically have a core of hydrophobic amino acids.[24] Her group has crystallized other ribozymes,[10] including the Hepatitis Delta Virus ribozyme.[24] This initial work to solve large RNA structures led to further structural studies on an internal ribosome entry site (IRES) and protein-RNA complexes such as the signal recognition particle.[24]

Doudna was promoted to the position of Henry Ford II Professor of Molecular Biophysics and Biochemistry at Yale in 2000. In 2000–2001, she was Robert Burns Woodward Visiting Professor of Chemistry at Harvard University.[5]

Move to Berkeley

edit
External videos
 
  "CRISPR Biology and Biotechnology: The Future of Genome Editing", Jennifer Doudna delivers the 29th annual Ullyot Public Affairs Lecture, Science History Institute

In 2002, she joined her husband, Jamie Cate, at Berkeley, accepting a position as professor of biochemistry and molecular biology.[10] Doudna also gained access to the synchrotron at Lawrence Berkeley National Laboratory for her experiments with high powered x-ray diffraction.[10]

In 2009, she took a leave of absence from Berkeley to work at Genentech to lead discovery research.[29] She left Genentech after two months and returned to Berkeley with the help of colleague Michael Marletta,[30] canceling all of her obligations to study CRISPR.[29]

As of 2023, Doudna was located at the University of California, Berkeley, where she directs the Innovative Genomics Institute, a collaboration between Berkeley and UCSF founded by Doudna to develop genome editing technology and apply it to some of society's greatest problems in human health, agriculture and climate change.[31][32][33] Doudna holds the Li Ka Shing Chancellor's Professorship in Biomedicine and Health, and is the chair of the Chancellor's Advisor Committee on Biology.[5] Her lab now focuses on the structure and function of CRISPR-Cas systems, developing new genome editing technology and delivery mechanisms for CRISPR therapeutics, and novel techniques for precisely editing microbiomes.[34][35][36][31]

CRISPR-Cas9 genome editing discovery

edit

Doudna was introduced to CRISPR by Jillian Banfield in 2006 who had found Doudna by way of a Google search, having typed "RNAi and UC Berkeley" into her browser, and Doudna's name came up at the top of the list.[37][38] In 2012, Doudna and her colleagues made a new discovery that reduces the time and work needed to edit genomic DNA.[39][40] Their discovery relies on a protein named Cas9 found in the Streptococcus bacterial "CRISPR" immune system that cooperates with guide RNA and works like scissors. The protein attacks its prey, the DNA of viruses, and slices it up, preventing it from infecting the bacterium.[7] This system was first discovered by Yoshizumi Ishino and colleagues in 1987[41] and later characterized by Francisco Mojica,[42] but Doudna and Emmanuelle Charpentier showed for the first time that they could use different RNAs to program it to cut and edit different DNAs.[7]

As CRISPR becomes increasingly used to edit multicellular organisms, Doudna continues to be called upon to serve as a thought-leader on the ethics of changing an organism's function using CRISPR technology.[43] Their discovery has since been further developed by many research groups[10] for applications ranging from fundamental cell biology, plant, and animal research to treatments for diseases including sickle cell anemia, cystic fibrosis, Huntington's disease, and HIV.[6][39] Doudna and several other leading biologists called for a worldwide moratorium on any clinical application of gene editing using CRISPR.[44][45] Doudna supports the usage of CRISPR in somatic gene editing, gene alterations which do not get passed to the next generation, but not germline gene editing.[46]

 
CRISPR-Cas9 complex

The CRISPR system created a new straightforward way to edit DNA and there was a rush to patent the technique.[6] Doudna and UC Berkeley collaborators applied for a patent and so did a group at the Broad Institute affiliated with the Massachusetts Institute of Technology and Harvard.[47] Feng Zhang at the Broad Institute had shown that CRISPR-Cas9 could edit genes in cultured human cells a few months after Doudna and Charpentier published their method.[39] Before the UC Berkeley patent application was decided, a patent was granted to the Broad investigators and UC Berkeley filed a lawsuit against the decision.[47] In 2017, the court decided in favor of the Broad Institute, who claimed that they had initiated the research earliest and had first applied it to human cell engineering thus supporting editing in human cells with evidence but that the UC Berkeley group had only suggested this application.[47] UC Berkeley appealed on grounds that they had clearly discussed and spelled out how to do the application the Broad had pursued.[48] In September 2018, the appeals court decided in favor of the Broad Institute's patent.[49] Meanwhile, UC Berkeley and co-applicants' patent to cover the general technique was also granted.[50] To further cloud the issue, in Europe the claim of the Broad Institute, to have initiated the research first, was disallowed.[51] The rejection was due to a procedural flaw in the application involving a different set of personnel listed in the lawsuit and the patent application, leading to speculation that the UC Berkeley group would prevail in Europe.[51] Doudna cofounded Caribou Biosciences, a company to commercialize CRISPR technology, in 2011.[52] In September 2013, Doudna cofounded Editas Medicine with Zhang and others despite their legal battles, but she quit in June 2014; Charpentier then invited her to join CRISPR Therapeutics, but she declined following the "divorce"-like experience at Editas.[53] Doudna is also a cofounder of Caribou spin-off Intellia Therapeutics[54][55] and Scribe Therapeutics, which pioneered CasX, a more compact, next-generation Cas9 which can efficiently cut DNA.[56]

In 2017, she co-authored A Crack in Creation: Gene Editing and the Unthinkable Power to Control Evolution,[6] a rare case of the first-person account of a major scientific breakthrough, aimed at the general public.[57]

In addition to the CRISPR breakthrough, Doudna has discovered that the hepatitis C virus utilizes an unusual strategy to synthesize viral proteins.[58] This work could lead to new drugs to stop infections without causing harm to the tissues of the body.[58]

"I have so much optimism about what CRISPR can do to help cure unaddressed genetic diseases and improve sustainable agriculture, but I'm also concerned that the benefits of the technology might not reach those who need it most if we're not thoughtful and deliberate about how we develop the technology," Doudna said.[19]

Mammoth Biosciences

edit

In 2017, Doudna co-founded Mammoth Biosciences,[59] a San Francisco-based bioengineering tech startup. Initial funding raised $23 million,[60] with a series B round of funding in 2020 raising $45 million.[61] The business is focused on improving access to bio sensing tests which address "challenges across healthcare, agriculture, environmental monitoring, biodefense, and more."[59]

COVID-19 response

edit

Beginning in March 2020, Doudna organized an effort to use CRISPR-based technologies to address the COVID-19 pandemic along with Dave Savage, Robert Tjian, and other colleagues at the Innovative Genomics Institute (IGI), where they created a testing center.[62] This center processed over 500,000 patient samples from UC Berkeley students, staff and faculty as well as members of the surrounding community and farm workers in the Salinas area.[63][64] Mammoth Biosciences announced a peer-reviewed validation of a rapid, CRISPR-based point of need COVID-19 diagnostic which is faster and less expensive than qRT-PCR based tests.[65]

Other activities

edit

She is also the founder and chair of the governance board of the Innovative Genomics Institute, which she co-founded in 2014.[36] Doudna is also a faculty scientist at Lawrence Berkeley National Laboratory, a senior investigator at the Gladstone Institutes, and an adjunct professor of cellular and molecular pharmacology at the University of California, San Francisco (UCSF).[66][24][67]

Doudna is on the scientific advisory boards of the companies that she cofounded, such as Caribou,[68] Intellia,[69] Mammoth,[70] and Scribe;[71] as well as others such as Altos Labs,[72] Isomorphic Labs,[73] Johnson & Johnson,[74] Synthego,[75] Tempus AI,[76] and Welch Foundation.[77] She joined Sixth Street Partners in 2022 as their chief science advisor, to guide investment decisions related to CRISPR.[78][79]

Personal life

edit

Doudna's first marriage was in 1988 to a fellow graduate student at Harvard named Tom Griffin, but his interests were more broad and less focused on research than hers and they divorced a few years later. Griffin wanted to move to Boulder, Colorado, where Doudna was also interested in working with Thomas Cech.[80] As a postdoctoral researcher at the University of Colorado, Doudna met Jamie Cate, then a graduate student. They worked together on the project to crystallize and determine the structure of the Tetrahymena Group I intron P4-P6 catalytic region. Doudna brought Cate with her to Yale, and they married in Hawaii in 2000. Cate later became a professor at the Massachusetts Institute of Technology and Doudna followed him to Boston at Harvard, but in 2002 they both accepted faculty positions at Berkeley and moved there together; Cate preferred the less formal environment on the West Coast from his earlier experiences at the University of California, Santa Cruz and the Lawrence Berkeley National Laboratory, and Doudna liked that Berkeley is a public university.[81] Cate is a Berkeley professor and works on gene-editing yeast to increase their cellulose fermentation for biofuel production. Doudna and Cate have a son born in 2002 who attends UC Berkeley, studying electrical engineering and computer science.[7] They live in Berkeley.[82]

Awards and honors

edit
Main article: List of awards and honors received by Jennifer Doudna

Doudna was a Searle Scholar and received the 1996 Beckman Young Investigators Award.[83][84][85] In 2000, she was awarded the Alan T. Waterman Award, the National Science Foundation's highest honor that annually recognizes an outstanding researcher under the age of 35, for her structure determination of a ribozyme.[9] In 2001, she received the Eli Lilly Award in Biological Chemistry of the American Chemical Society.[5]

In 2015, together with Emmanuelle Charpentier, she received the Breakthrough Prize in Life Sciences for her contributions to CRISPR/Cas9 genome editing technology.[86] In 2016, together with Charpentier, Feng Zhang, Philippe Horvath and Rodolphe Barrangou, she received the Canada Gairdner International Award.[13] Also in 2016, she received the Heineken Prize for Biochemistry and Biophysics.[87] She has also been a co-recipient of the Gruber Prize in Genetics (2015),[88] the Tang Prize (2016),[12] the Japan Prize (2017) and the Albany Medical Center Prize (2017).[89] In 2018, Doudna was awarded the NAS Award in Chemical Sciences,[90] the Pearl Meister Greengard Prize from the Rockefeller University,[91] and a Medal of Honor from the American Cancer Society.[92] Also in 2018, she was awarded the Kavli Prize in Nanoscience (jointly with Emmanuelle Charpentier and Virginijus Šikšnys).[93][94] In 2019 she received the Harvey Prize of the Technion/Israel for the year 2018 (jointly with Emmanuelle Charpentier and Feng Zhang)[95] and the LUI Che Woo Prize in the category of Welfare Betterment.[96] In 2020, she received the Wolf Prize in Medicine (jointly with Emmanuelle Charpentier).[97] Also in 2020, Doudna and Charpentier were awarded the Nobel Prize in Chemistry "for the development of a method for genome editing."[4][3]

She was elected to the National Academy of Sciences in 2002,[98][24] the American Academy of Arts and Sciences in 2003,[99] the National Academy of Medicine in 2010 and the National Academy of Inventors in 2014.[5] In 2015, together with Charpentier, she became a fellow of the American Academy of Microbiology.[100] She was elected a Foreign Member of the Royal Society (ForMemRS) in 2016.[101] In 2017, Doudna was awarded the Golden Plate Award[102] of the American Academy of Achievement.[103] In 2020, she was awarded a Guggenheim Fellowship.[104] In 2021 she received the Award for Excellence in Molecular Diagnostics from the Association for Molecular Pathology.[105] In 2021, Pope Francis appointed Doudna, and two other female Nobel laureates Donna Strickland and Emmanuelle Charpentier, as members of the Pontifical Academy of Sciences.[106]

She along with Charpentier was named one of the Time 100 most influential people in 2015,[15] and she was a runner-up for Time Person of the Year in 2016 alongside other CRISPR researchers.[39] In 2018 and 2023, she received honorary Doctor of Science degrees from USC[107] and Harvard,[108] respectively.

References

edit
  1. ^ "Pondering 'what it means to be human' on the frontier of gene editing". The Washington Post. May 3, 2016. Archived from the original on October 15, 2020. Retrieved October 14, 2020.
  2. ^ a b "Jennifer Doudna – American biochemist". Encyclopædia Britannica Online. Archived from the original on October 7, 2020. Retrieved November 13, 2015.
  3. ^ a b Wu, Katherine J.; Zimmer, Carl; Peltier, Elian (October 7, 2020). "Nobel Prize in Chemistry Awarded to 2 Scientists for Work on Genome Editing". The New York Times. Archived from the original on October 8, 2020. Retrieved October 7, 2020.
  4. ^ a b "Press release: The Nobel Prize in Chemistry 2020". nobelprize.org. Nobel Foundation. October 7, 2020. Archived from the original on January 15, 2021. Retrieved October 7, 2020.
  5. ^ a b c d e f g h i "Curriculum Vitae (Jennifer A. Doudna)" (PDF). Lawrence Berkeley National Laboratory. Archived (PDF) from the original on January 15, 2017. Retrieved October 24, 2017.
  6. ^ a b c d e f g h i j k l m n Jennifer A. Doudna and Samuel H. Sternberg. A Crack in Creation: Gene Editing and the Unthinkable Power to Control Evolution. Houghton Mifflin Harcourt, 2017.
  7. ^ a b c d e f g h i j Russell, Sabin (December 8, 2014). "Cracking the Code: Jennifer Doudna and Her Amazing Molecular Scissors". Cal Alumni Association. Archived from the original on September 20, 2020. Retrieved November 10, 2017.
  8. ^ Pollack, Andrew (May 11, 2015). "Jennifer Doudna, a Pioneer Who Helped Simplify Genome Editing". The New York Times. Archived from the original on January 2, 2022. Retrieved May 12, 2015.
  9. ^ a b "Alan T. Waterman Award Recipients, 1976 – present". National Science Foundation. Archived from the original on March 2, 2015. Retrieved October 31, 2017.
  10. ^ a b c d e f g h "Laureates: Jennifer A. Doudna". breakthroughprize.org. Archived from the original on April 20, 2022. Retrieved October 31, 2017.
  11. ^ "2015 Genetics Prize: Jennifer Doudna". The Gruber Foundation. Archived from the original on September 5, 2016. Retrieved October 24, 2017.
  12. ^ a b "Laureates: Biopharmaceutical Science (2016)". Tang Prize Foundation. Archived from the original on November 7, 2017. Retrieved November 1, 2017.
  13. ^ a b "Jennifer Doudna". Canada Gairdner Foundation. Archived from the original on June 12, 2018. Retrieved November 2, 2017.
  14. ^ "Laureates of the Japan Prize: Jennifer A. Doudna, Ph.D." The Japan Prize Foundation. Archived from the original on November 7, 2017. Retrieved November 1, 2017.
  15. ^ a b King, Mary-Claire (April 16, 2015). "Time 100 Most Influential People: Emmanuelle Charpentier & Jennifer Doudna". Time. Archived from the original on December 29, 2016. Retrieved December 26, 2016.
  16. ^ "2023 Inductee Jennifer Doudna | National Inventors Hall of Fame®". www.invent.org. December 28, 2023. Retrieved December 29, 2023.
  17. ^ Who's who in the West. Marquis-Who's Who. 1999. ISBN 9780837909240.
  18. ^ a b c d Mukhopadyay, Rajendrani. "On the same wavelength". American Society for Biochemistry and Molecular Biology. Archived from the original on December 31, 2019. Retrieved October 24, 2017.
  19. ^ a b "Becoming a Scientific Leader". The Women in Medicine Legacy Foundation. September 8, 2021. Archived from the original on December 10, 2022. Retrieved December 10, 2022.
  20. ^ Cataluna, Lee (October 11, 2020). "Remembering The Hilo Teacher Who Inspired A Nobel Prize Winner". Honolulu Civil Beat. Archived from the original on November 13, 2022. Retrieved October 22, 2020.
  21. ^ "Big Questions, Big Answers". SBGrid Consortium. Archived from the original on November 13, 2022. Retrieved October 22, 2020.
  22. ^ "2018 Kavli Prize in Nanoscience: A Conversation with Jennifer Doudna, Emmanuelle Charpentier and Virginijus Šikšnys". The Kavli Prize. Retrieved October 22, 2020.
  23. ^ "Genome editing pioneer and Hilo High graduate Jennifer Doudna speaks at UH Hilo about her discovery: CRISPR technology". UH Hilo Stories. September 19, 2018. Retrieved October 7, 2020.
  24. ^ a b c d e f g h i j k l m n o Melissa Marino (December 1, 2004). "Biography of Jennifer A. Doudna". Proceedings of the National Academy of Sciences of the United States of America. 101 (49): 16987–16989. Bibcode:2004PNAS..10116987M. doi:10.1073/PNAS.0408147101. ISSN 0027-8424. PMC 535403. PMID 15574498. Wikidata Q34553023.
  25. ^ Engineer, Anushe (October 7, 2020). "Pomona College alumna wins Nobel Prize in Chemistry". The Student Life. Archived from the original on October 24, 2020. Retrieved October 8, 2020.
  26. ^ Doudna, Jennifer Anne (1989). Towards the Design of an RNA Replicase (Ph.D. thesis). Harvard University. OCLC 23230360. ProQuest 303754572.
  27. ^ Jennifer Doudna publications indexed by Google Scholar  
  28. ^ "Scopus preview – Doudna, Jennifer A. – Author details – Scopus". www.scopus.com. Archived from the original on January 20, 2022. Retrieved October 15, 2021.
  29. ^ a b "A DAY WITH JENNIFER DOUDNA: TRYING TO KEEP UP WITH ONE OF THE WORLD'S MOST SOUGHT-AFTER SCIENTISTS". March 8, 2020. Archived from the original on April 12, 2020. Retrieved May 2, 2020.
  30. ^ Isaacson 2021, p. 101.
  31. ^ a b "CRISPR pioneer Doudna envisions ending asthma, aiding climate". Marketplace. Retrieved December 29, 2023.
  32. ^ "How Jennifer Doudna's Life Has Changed Since Discovering CRISPR 10 Years Ago". TIME. July 1, 2022. Retrieved December 29, 2023.
  33. ^ "About Us". Innovative Genomics Institute (IGI). Retrieved December 29, 2023.
  34. ^ "Structure and Mechanism". Doudna Lab. Retrieved December 29, 2023.
  35. ^ "IGI's 'Audacious' New Frontier for CRISPR: Editing Microbiomes for Climate and Health". Innovative Genomics Institute (IGI). Retrieved December 29, 2023.
  36. ^ a b "Jennifer Doudna". Innovative Genomics Institute (IGI). Retrieved December 29, 2023.
  37. ^ Jill Banfield: How a curious Google search led me to Jennifer Doudna Archived October 12, 2020, at the Wayback Machine, Jill Banfield (Berkeley News, published October 7, 2020)
  38. ^ Jennifer Doudna's First Reactions to 2020 Nobel Prize Win Archived November 20, 2021, at the Wayback Machine (published October 7, 2020, to the UC Berkeley channel on YouTube)
  39. ^ a b c d Park, Alice (2016). "The CRISPR Pioneers: Their Breakthrough Work Could Change the World". Time. Archived from the original on December 25, 2016. Retrieved December 26, 2016.
  40. ^ Martin Jínek; Krzysztof Chylinski; Ines Fonfara; Michael Hauer; Jennifer A Doudna; Emmanuelle Charpentier (June 28, 2012). "A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity". Science. 337 (6096): 816–821. Bibcode:2012Sci...337..816J. doi:10.1126/SCIENCE.1225829. ISSN 0036-8075. PMC 6286148. PMID 22745249. Wikidata Q24669850.
  41. ^ Yoshizumi Ishino; H Shinagawa; K Makino; M Amemura; A Nakata (December 1987). "Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product". Journal of Bacteriology. 169 (12): 5429–33. doi:10.1128/JB.169.12.5429-5433.1987. ISSN 0021-9193. PMC 213968. PMID 3316184. Wikidata Q24679073.
  42. ^ Francisco Juan Martínez Mojica; Lluis Montoliu (July 9, 2016). "On the Origin of CRISPR-Cas Technology: From Prokaryotes to Mammals". Trends in Microbiology. 24 (10): 811–820. doi:10.1016/J.TIM.2016.06.005. ISSN 0966-842X. PMID 27401123. Wikidata Q34533807.
  43. ^ "CRISPR co-inventor Jennifer Doudna talks ethics and biological frontiers". January 25, 2019. Archived from the original on March 8, 2019. Retrieved March 16, 2019.
  44. ^ Wade, Nicholas (March 19, 2015). "Scientists Seek Ban on Method of Editing the Human Genome". The New York Times. Archived from the original on May 31, 2020. Retrieved June 10, 2020.
  45. ^ David Baltimore; Paul Berg; Michael Botchan; et al. (April 3, 2015). "Biotechnology. A prudent path forward for genomic engineering and germline gene modification". Science. 348 (6230): 36–8. Bibcode:2015Sci...348...36B. doi:10.1126/SCIENCE.AAB1028. ISSN 0036-8075. PMC 4394183. PMID 25791083. Wikidata Q22065510.
  46. ^ "CRISPR's co-developer on the revolutionary gene-editing technology's past — and its future". February 28, 2020. Archived from the original on July 30, 2020. Retrieved June 10, 2020.
  47. ^ a b c Netburn, Deborah (February 15, 2017). "UC Berkeley suffers big loss in CRISPR patent fight: What's next for the gene-editing technology?". Los Angeles Times. Retrieved September 2, 2018.
  48. ^ Decker, Susan; Cortez, Michelle (April 28, 2018). "This court battle will decide who will make a fortune from gene-editing technique". Bloomberg. Archived from the original on May 9, 2018. Retrieved September 2, 2018.
  49. ^ Jeff Akst, (2018) "The higher court's decision to uphold the ruling of the Patent Trial and Appeal Board essentially ends the intellectual property battle in theUS" The Scientist, September 10, 2018.
  50. ^ Paganelli, Jennifer (June 19, 2018). "CRISPR Therapeutics, Intellia Therapeutics, and Caribou Biosciences announce grant of US patent for CRISPR/Cas9 Genome Editing". Caribou Biosciences, Inc. Archived from the original on September 12, 2018. Retrieved September 2, 2018.
  51. ^ a b Servick, Kelly (January 18, 2018). "Broad Institute takes a hit in European CRISPR patent struggle". Science. Archived from the original on March 1, 2022. Retrieved September 2, 2018.
  52. ^ "Caribou Biosciences, Inc". Bloomberg business. Archived from the original on September 12, 2018. Retrieved September 2, 2018.
  53. ^ Isaacson 2021, pp. 208–213.
  54. ^ "Caribou Biosciences Announces Co-Founding of Intellia Therapeutics". www.businesswire.com. November 18, 2014. Archived from the original on May 16, 2021. Retrieved May 16, 2021.
  55. ^ "Intellia Therapeutics Congratulates Co-Founder Jennifer Doudna On Winning the 2020 Nobel Prize in Chemistry for Inventing the Revolutionary CRISPR/Cas9 Genome Editing Technology". finance.yahoo.com. October 7, 2020. Archived from the original on May 16, 2021. Retrieved May 16, 2021.
  56. ^ "A day with Jennifer Doudna: Trying to keep up with one of the world's most sought-after scientists". Chemical & Engineering News. Archived from the original on April 12, 2020. Retrieved May 2, 2020.
  57. ^ "A Crack in Creation review – Jennifer Doudna, CRISPR and a great scientific breakthrough". TheGuardian.com. June 17, 2017. Archived from the original on September 27, 2020. Retrieved October 8, 2020.
  58. ^ a b "Jennifer A. Doudna, Ph.D." HHMI. Archived from the original on May 16, 2013. Retrieved August 26, 2012.
  59. ^ a b "Mammoth Biosciences | About Us". Mammoth Biosciences. Archived from the original on April 22, 2020. Retrieved April 14, 2020.
  60. ^ "Mammoth Biosciences Closes on Series A Worth $23 Million". BioSpace. August 1, 2019. Archived from the original on April 15, 2020. Retrieved April 14, 2020.
  61. ^ "Mammoth Biosciences Raises $45 Million to Build Next Generation CRISPR Products For Therapeutics and Diagnostics". BioSpace. January 30, 2020. Archived from the original on July 21, 2020. Retrieved April 14, 2020.
  62. ^ Isaacson 2021, p. 401.
  63. ^ "Half a Million COVID Tests Later, IGI Clinical Laboratory Looks to the Future". Innovative Genomics Institute (IGI). Retrieved December 29, 2023.
  64. ^ "CRISPR pioneer Doudna opens COVID-19 testing lab – BioNews". www.bionews.org.uk. April 3, 2020. Archived from the original on May 10, 2020. Retrieved May 2, 2020.
  65. ^ Release, Press (April 21, 2020). "Mammoth Biosciences Announces Peer-Reviewed Validation Of Its Rapid, CRISPR-Based COVID-19 Diagnostic". SynBioBeta. Archived from the original on April 25, 2020. Retrieved May 2, 2020.
  66. ^ Multiple sources:
  67. ^ Jennifer Doudna's publications indexed by the Scopus bibliographic database. (subscription required)
  68. ^ Leuty, Ron (July 1, 2021). "Jennifer Doudna's first East Bay CRISPR spinout files for IPO". San Francisco Business Times. Archived from the original on March 14, 2024.
  69. ^ Darie, Tatiana (October 19, 2019). "Crispr's Next Frontier Is In-Human Treatment, Co-Inventor Says". Bloomberg News. Archived from the original on October 20, 2019.
  70. ^ Fannin, Rebecca (March 12, 2022). "The 30-year-old female founder at the forefront of a billion-dollar bet on CRISPR gene editing". CNBC. Archived from the original on December 10, 2023.
  71. ^ Knapp, Alex (July 25, 2023). "This Scientist Is Building Custom Gene-Editing Tools—And Stands To Make Billions". Forbes. Archived from the original on July 25, 2023.
  72. ^ Regalado, Antonio (September 4, 2021). "Meet Altos Labs, Silicon Valley's latest wild bet on living forever". MIT Technology Review. Archived from the original on March 4, 2024.
  73. ^ Bayer, Max (January 7, 2024). "Alphabet's Isomorphic stacks two new deals with Lilly, Novartis worth nearly $3B ahead of JPM". Fierce Biotech. Archived from the original on March 5, 2024.
  74. ^ Kuchler, Hannah (January 31, 2020). "Jennifer Doudna, Crispr scientist, on the ethics of editing humans". Financial Times. Archived from the original on January 31, 2020.
  75. ^ Molteni, Megan (November 30, 2018). "US Biotech Firms Made China's Gene-Edited Babies Possible". Wired. Archived from the original on February 13, 2024.
  76. ^ Tan, Gillian (November 2, 2021). "Tempus, Groupon Co-Founder's Medical Technically Startup, Is Exploring an IPO". Bloomberg News. Archived from the original on November 2, 2021.
  77. ^ Seijo, Bibiana Campos (October 26, 2019). "The Welch Foundation supports basic research in chemistry in Texas". Chemical & Engineering News. Archived from the original on March 14, 2024.
  78. ^ Regalado, Antonio (April 26, 2022). "The scientist who co-created CRISPR isn't ruling out engineered babies someday". MIT Technology Review. Archived from the original on December 22, 2023.
  79. ^ Goodwin, Kate (February 8, 2022). "CRISPR Pioneer Doudna Joins Global Investment Firm as CSO". BioSpace. Archived from the original on April 1, 2022.
  80. ^ Isaacson 2021, pp. 54–55.
  81. ^ Isaacson 2021, pp. 63–66.
  82. ^ Kreiger, Lisa M. (October 7, 2020). "Nobel Prize: UC Berkeley's Jennifer Doudna wins for pioneering gene-editing tool". The Mercury News. Archived from the original on February 4, 2023.
  83. ^ Read "Enhancing Philanthropy's Support of Biomedical Scientists: Proceedings of a Workshop on Evaluation" at NAP.edu. 2006. doi:10.17226/11646. ISBN 978-0-309-10097-7.
  84. ^ "Beckman Young Investigators Award Recipients". Arnold and Mabel Beckman Foundation. Archived from the original on September 11, 2019. Retrieved November 6, 2017.
  85. ^ "Jennifer A. Doudna". Arnold and Mabel Beckman Foundation. Archived from the original on September 13, 2019. Retrieved August 1, 2018.
  86. ^ "Jennifer Doudna". Breakthrough Prize. Archived from the original on April 20, 2022. Retrieved September 13, 2018.
  87. ^ "Jennifer Doudna". The Royal Netherlands Academy of Arts and Sciences. Archived from the original on May 13, 2016. Retrieved November 6, 2017.
  88. ^ "2015 Genetics Prize: Jennifer Doudna". The Gruber Foundation. Archived from the original on September 5, 2016. Retrieved October 24, 2017.
  89. ^ "Gene Editing Pioneers Selected to Receive America's Most Distinguished Prize in Medicine". Albany Medical Center. Archived from the original on February 3, 2019. Retrieved November 1, 2017.
  90. ^ "National Academy of Sciences Awards". National Academy of Sciences. Archived from the original on September 13, 2018. Retrieved September 13, 2018.
  91. ^ "Jennifer Doudna To Receive 2018 Pearl Meister Greengard Prize". Rockefeller University. Archived from the original on September 14, 2018. Retrieved September 13, 2018.
  92. ^ Sanders, Robert (October 18, 2018). "Doudna receives Medal of Honor from American Cancer Society". Berkeley News. Archived from the original on August 2, 2020. Retrieved March 16, 2019.
  93. ^ Giorgia Guglielmi (June 1, 2018). "Million-dollar Kavli prize recognizes scientist scooped on CRISPR". Nature. 558 (7708): 17–18. doi:10.1038/D41586-018-05308-5. ISSN 1476-4687. PMID 29872189. Wikidata Q74441382.
  94. ^ Cohen, Jon (June 4, 2018). "With prestigious prize, an overshadowed CRISPR researcher wins the spotlight". Science. American Association for the Advancement of Science (AAAS). doi:10.1126/science.aau3736. ISSN 0036-8075. S2CID 158427487.
  95. ^ "Harvey Prize 2018". October 7, 2019. Archived from the original on November 9, 2019. Retrieved November 9, 2019.
  96. ^ "LUI Che Woo Prize Reveals 2019 Laureates – Furthering Its Mission to Enrich World Civilisation". luiprize.org. Archived from the original on January 29, 2020. Retrieved November 12, 2019.
  97. ^ "Wolf Prize 2020". Archived from the original on January 12, 2020. Retrieved January 13, 2020.
  98. ^ "Jennifer A. Doudna". www.nasonline.org. Archived from the original on November 13, 2023. Retrieved November 13, 2023.
  99. ^ "Jennifer A. Doudna". American Academy of Arts & Sciences. October 31, 2023. Archived from the original on November 13, 2023. Retrieved November 13, 2023.
  100. ^ "Nobel Prize Awarded to ASM Members for Development of CRISPR-Cas9". American Society for Microbiology. Archived from the original on October 18, 2021. Retrieved October 18, 2021.
  101. ^ Anon (2016). "Professor Jennifer Doudna ForMemRS". London: Royal Society. Archived from the original on April 29, 2016. Retrieved April 29, 2016. One or more of the preceding sentences incorporates text from the royalsociety.org website where:

    "All text published under the heading 'Biography' on Fellow profile pages is available under Creative Commons Attribution 4.0 International License." --"Royal Society Terms, conditions and policies". Archived from the original on September 25, 2015. Retrieved March 9, 2016.
  102. ^ "Golden Plate Awardees of the American Academy of Achievement". achievement.org. American Academy of Achievement. Archived from the original on December 12, 2017. Retrieved June 26, 2019.
  103. ^ "Jennifer A. Doudna, Ph.D. Biography and Interview". achievement.org. American Academy of Achievement. Archived from the original on August 12, 2020. Retrieved June 26, 2019.
  104. ^ "John Simon Guggenheim Foundation | Jennifer Doudna". Archived from the original on April 13, 2020. Retrieved October 15, 2021.
  105. ^ "Past Recipients". Association for Molecular Pathology. Archived from the original on March 27, 2023. Retrieved April 12, 2023.
  106. ^ Dulle, Colleen (August 18, 2021). "Pope Francis appointed three women to the Pontifical Academy of Sciences this summer. What's their role at the Vatican?". America Magazine. Archived from the original on November 30, 2021. Retrieved October 15, 2021.
  107. ^ "Past Recipients – Honorary Degrees". honorarydegrees.usc.edu. Retrieved June 6, 2024.
  108. ^ "Harvard awards six honorary degrees". news.harvard.edu. May 25, 2023. Retrieved April 28, 2024.

Bibliography

edit
edit
 
Scholia has an author profile for Jennifer Doudna.
 
Wikiquote has quotations related to Jennifer Doudna.
Retrieved from "https://en.wikipedia.org/w/index.php?title=Jennifer_Doudna&oldid=1257916779"