Abstract
The murine mortalin genes, mot-1 and mot-2, are members of the hsp70 family of proteins and differ from each other by only two amino acid residues. Mot-1 is expressed in normal cells and has pancytosolic cellular distribution whereas mot-2 is found in the perinuclear region of immortal cells. We report here that a high level of expression of mot-2 protein resulted in malignant transformation of cells as analysed by anchorage independent growth and nude mice assays. A high level of protein expression is attributed to the 900 bp 3′ untranslated region of the cDNA which does not have any transforming activity per se. Mortalin cDNA clones isolated from human transformed cells were also found to have transforming activity in similar assays and a high level of expression was apparent in some of the human immortalized cells that showed non-pancytosolic mortalin immunofluorescence. Taken together, the data suggest that nonpancytosolic mortalin may have a role in tumorigenesis.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kaul, S., Duncan, E., Englezou, A. et al. Malignant transformation of NIH3T3 cells by overexpression of mot-2 protei. Oncogene 17, 907–911 (1998). https://doi.org/10.1038/sj.onc.1202017
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1202017
Keywords
This article is cited by
-
Anti-diabetic vanadyl complexes reduced Alzheimer’s disease pathology independent of amyloid plaque deposition
Science China Life Sciences (2019)
-
Mortalin deficiency suppresses fibrosis and induces apoptosis in keloid spheroids
Scientific Reports (2017)
-
Ubiquitin-like (UBX)-domain-containing protein, UBXN2A, promotes cell death by interfering with the p53-Mortalin interactions in colon cancer cells
Cell Death & Disease (2014)
-
Glucose-regulated proteins in cancer: molecular mechanisms and therapeutic potential
Nature Reviews Cancer (2014)
-
Mortalin is a prognostic factor of gastric cancer with normal p53 function
Gastric Cancer (2014)