On-Site Detection of Aflatoxin B1 in Grains by a Palm-Sized Surface Plasmon Resonance Sensor
<p>(<b>A</b>) Photograph of the portable surface plasmon resonance (SPR) system combined with a syringe pump and laptop. The Au chip was installed in the portable SPR system, and the sample solution was injected with the syringe pump. The SPR response was displayed on the laptop. (<b>B</b>) Schematic illustration of the portable SPR system based on the modulation of laser light by a rotating mirror.</p> "> Figure 2
<p>Schematic illustration of aflatoxin B1 (AFB1) detection using the portable SPR sensor.</p> "> Figure 3
<p>(<b>A</b>) SPR response curves obtained in the control channel (black line) and test channels by varying the AFB1 concentration (1, 10, 100, 1000, and 10,000 ppb). The SPR response intensity decreased with increasing AFB1 concentration because the amount of bound AFB1-BSA is inversely proportional to the AFB1 concentration. (<b>B</b>) Plot of (<span class="html-italic">I</span><sub>C</sub> − <span class="html-italic">I</span><sub>T</sub>)/<span class="html-italic">I</span><sub>C</sub> versus the concentration of AFB1. <span class="html-italic">I</span><sub>C</sub> and <span class="html-italic">I</span><sub>T</sub> represent the mean SPR response intensity in the control and test channels, respectively. The red linearly fitted line. The data represent the mean plus standard deviation from three measurements.</p> "> Figure 4
<p>SPR response curves obtained in test channels after the detection of AFB1 and zearalenone, respectively. The concentration of both mycotoxins is 100 ppb. The SPR response was higher in the presence of zearalenone than in the presence of AFB1, indicating the selective detection of AFB1.</p> "> Figure 5
<p>(<b>A</b>) Photograph of the AFB1-spiked rice, peanut, and almond samples. (<b>B</b>) Plot of (<span class="html-italic">I</span><sub>C</sub> − <span class="html-italic">I</span><sub>T</sub>)/<span class="html-italic">I</span><sub>C</sub> versus the grain sample. The blue bars were obtained from the AFB1-spiked grain samples, and the black bars from pure grain samples. <span class="html-italic">I</span><sub>C</sub> and <span class="html-italic">I</span><sub>T</sub> represent the mean SPR response intensity in the control and test channels, respectively.</p> ">
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of AFB1-Spiked Food Samples
2.3. Preparation of the Au Chip and Detection of AFB1 by the Portable SPR Sensor
2.4. Instrumentation
3. Results and Discussion
3.1. Detection of AFB1 by the Portable SPR Sensor
3.2. Quantitative and Selective Detection of AFB1
3.3. The On-Site Detection of AFB1 in Grains
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Moon, J.; Byun, J.; Kim, H.; Lim, E.-K.; Jeong, J.; Jung, J.; Kang, T. On-Site Detection of Aflatoxin B1 in Grains by a Palm-Sized Surface Plasmon Resonance Sensor. Sensors 2018, 18, 598. https://doi.org/10.3390/s18020598
Moon J, Byun J, Kim H, Lim E-K, Jeong J, Jung J, Kang T. On-Site Detection of Aflatoxin B1 in Grains by a Palm-Sized Surface Plasmon Resonance Sensor. Sensors. 2018; 18(2):598. https://doi.org/10.3390/s18020598
Chicago/Turabian StyleMoon, Jeong, Jihyun Byun, Hongki Kim, Eun-Kyung Lim, Jinyoung Jeong, Juyuen Jung, and Taejoon Kang. 2018. "On-Site Detection of Aflatoxin B1 in Grains by a Palm-Sized Surface Plasmon Resonance Sensor" Sensors 18, no. 2: 598. https://doi.org/10.3390/s18020598