ARF6 mediates nephrin tyrosine phosphorylation-induced podocyte cellular dynamics
Fig 1
ARF6 is enriched in human glomerular podocytes.
A. Sections of normal human kidney obtained by nephrectomy were stained by immunohistochemistry for ARF6 (brown). Negative control (right). Scale bar 30 μm. B. Indirect immunofluorescence confocal microscopy was used to image normal human kidney tissue stained for ARF6 (green) and nephrin (red). Magnification x63. Scale bar 11μm. C. Stimulated emission depletion micrograph of normal human kidney tissue stained for ARF6 (green) and nephrin (red). Scale bar 5 μm. D. Immunoblot analysis of ARF6 from lysate prepared from 1) normal wild-type (WT) mouse kidney, 2) isolated rat glomeruli, and 3) human podocyte cell line. β-actin was used as loading control.