Mechanism of Action of Two Flavone Isomers Targeting Cancer Cells with Varying Cell Differentiation Status
Fig 5
Analysis of caspase 9 after treatment with flavone A.
Detection of activated caspase 9 by immunoblot of SDS extracts of A. CaCo 2 and B. Panc 28 cells 1.5, 3, 6, 9 and 12 hours (lanes 2–6) after treatment with flavone A or vehicle (DMSO) for the control (C, lane 1) and SDS-PAGE. The membranes were probed with an antibody capable of detecting both the procaspase (47 kDa) and the large fragments resultant after activation (37 and 35 kDa). The membranes were reprobed for actin or tubulin as a loading control. The results shown are representative of three independent experiments. The membranes were reprobed for actin or tubulin as a loading control. The results shown are representative of three independent experiments.