A Laser-Induced Mouse Model with Long-Term Intraocular Pressure Elevation
Figure 5
RGC and optic nerve axonal damage following IOP elevation.
A and B represent images of plastic-embedded retinal sagittal sections peripheral to the optic nerve with hemotoxylin-eosin staining. A: Laser-treated. B: Normal control. C: Statistical analysis of the RGC number counts (p<0.001, paired t-test). D and E show toluidine blue staining of semithin transverse sections of optic nerves. D: Laser treated eye with swollen axons (arrowheads) and shrunken axons (arrows). E: Normal control with more homogeneous axons. D1–E1 are magnified views of the regions encased in black boxes in D–E. F Represents the statistical analysis of the axons (p<0.0001, paired t-test). RNFL, retinal nerve fiber layer; RGC, retinal ganglion cells; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer.