Diosgenin, a Steroidal Saponin, Inhibits Migration and Invasion of Human Prostate Cancer PC-3 Cells by Reducing Matrix Metalloproteinases Expression
Figure 9
Effects of diosgenin on NF-κB activation.
PC-3 cells were treated with various doses of diosgenin for 24 h. (A) The cytosolic and nuclear extracts were prepared and analyzed for IκBα degradation and NF-κB p65 translocation. β-actin and C23 were used as a cytosolic and nuclear protein loading control, respectively. (B) Determined levels of IκBα and NF-κB were quantified by densitometric analysis. The densitometric results were expressed as mean ± S.D. of three independent experiments. *p<0.05, **p<0.01, ***p<0.001, compared with the untreated control.