PKA activity is essential for relieving the suppression of hyphal growth and appressorium formation by MoSfl1 in Magnaporthe oryzae
Fig 3
Phenotypes of the spontaneous suppressor strain CCS1 of cpkA cpk2.
A. Seven-day-old OTA cultures of the wild type strain Guy11, cpkA cpk2 mutant, and suppressor strain CCS1. The fast-growing suppressor of cpkA cpk2 was marked with an arrow. B. Hyphal tips of Guy11 and CCS1 from 7-day-old OTA cultures. Arrows point to melanized hyphal tips. Scale bar = 10 μm. C. Rice leaves inoculated by injection with conidia of Guy11 and CCS1 were examined 7 days-post-inoculation. D. Appressoria formed by Guy11 and CCS1 on the hydrophobic and hydrophilic surfaces after incubation for 24 h. Scale bar = 10 μm. E. Abnormal appressoria formed by CCS1 on the hydrophobic surface after incubation for 24 h. Scale bar = 10 μm.