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- Agriculture Biotechnology Professional Mentor for Industrial, Entrepreneurship and Research Projects. Leading FloraFa... moreAgriculture Biotechnology Professional Mentor for Industrial, Entrepreneurship and Research Projects. Leading FloraFauna Science Foundation for Societal Enabling in Health, Agriculture and Nutrition Sectors to Transform Villagers into Entrepreneurs by Technology Innovations.edit
The highly polymorphic human alpha-1 antitrypsin (AAT) gene codes for the most abundant circulating plasma serine protease inhibitor. Previously, genetic variants of the AAT gene were reported from different regions of the world. In the... more
The highly polymorphic human alpha-1 antitrypsin (AAT) gene codes for the most abundant circulating plasma serine protease inhibitor. Previously, genetic variants of the AAT gene were reported from different regions of the world. In the present study, the AAT gene was characterized in an Indian sample. The AAT gene was isolated and cloned from a liver biopsy sample through RT-PCR and the full-length gene was sequenced. Nucleotide sequence comparison with the human genome and the AAT sequences available in the GenBank (NCBI) demonstrated four unique variations--(i) an A to G variation at position 286 (Thr96Ala), (ii) an A to G variation at position 839 (Asp280Gly), (iii) a T to C variation at position 1182 that did not result in any change in the protein sequence (TTT to TTC both code for Phe) and (iv) an A to C variation at position 1200 (Glu400Asp) that resulted in replacement by an amino acid of similar nature. Other variations found were T to C at position 710 (Val273Ala) and T t...
Research Interests: Biology, Medicine, Biopsy, India, Humans, and 14 moreLiver, Indian, Human Genome, Genetic Polymorphism, Genetic variation, RT PCR, Codon, Amino Acid Sequence, Base Sequence, Alpha-1 Antitrypsin, Biochemistry and cell biology, Molecular Sequence Data, alleles, and reverse transcriptase polymerase chain reaction
The presence of certain metabolites has been observed to interfere with DNA isolation procedures and downstream reactions such as DNA restriction, amplification and cloning. The chemotypic heterogeneity among species may not permit... more
The presence of certain metabolites has been observed to interfere with DNA isolation procedures and downstream reactions such as DNA restriction, amplification and cloning. The chemotypic heterogeneity among species may not permit optimal DNA yields with a single protocol, and thus, even closely related species may require different isolation protocols. Here we describe the essential steps of a rapid DNA
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Research Interests:
Due to the high demand and low yield of the anti-malarial drug artemisinin in natural populations of Artemisia annua (Quinghao), an attempt has been made to enhance the artemisinin content through 4 cycles of recurrent selection... more
Due to the high demand and low yield of the anti-malarial drug artemisinin in natural populations of Artemisia annua (Quinghao), an attempt has been made to enhance the artemisinin content through 4 cycles of recurrent selection (C(0)-C(3)) using selected genotypic and phenotypic traits. Based on their phenotypic and genotypic characteristics, the top 5% plants of each cycle were selected, and their seedlings were planted in poly-cross block to produce seeds for the subsequent generation. A significant increase in the artemisinin content (0.15% in C (0) to 1.16% in C (3), i.e., about 40% genetic gain over the generation) was observed. This enhancement was directly correlated with the plant height and branching intensity in all four cycles. Similarly, the PCV (phenotypic coefficient of variation) and GCV (genotypic coefficient of variation) have been observed to have a higher value for artemisinin content. The DNA marker (MAP 12) with relation to artemisinin was also identified for high yielding genotypes in all four cycles of selection. Over the four cycles of recurrent selection, the plant developed an oval appearance (Variety: CIM-Arogya) and a high artemisinin content (1.16%).
Research Interests: Complementary and Alternative Medicine, Plant Biology, Biology, Breeding, Pharmacognosy, and 15 moreMedicine, Correlation, Artemisia, Phenotype, Plant extracts, Genotype, Seeds, Seedling, Molecular Marker, Antimalarials, Artemisia Annua, Artemisinin, Genetic Markers, Planta, and Pharmacology and pharmaceutical sciences
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Research Interests:
Research Interests: Pharmacology, Chemistry, Complementary and Alternative Medicine, Plant Biology, Medicine, and 15 morePhytomedicine, Phytotherapy, Animals, Male, Plant extracts, Rats, Carbon Tetrachloride, Phyllanthus, Combination drug therapy, Aqueous Extract, Drug Induced Liver Injury, CCL, Combination Therapy, Drug synergism, and Pharmacology and pharmaceutical sciences
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Twenty-two endophytic bacterial isolates from the roots of sugarcane were compared morphologically, biochemically and genetically. Gram staining, colony pigment, texture and other cultural characteristics were taken for morphological... more
Twenty-two endophytic bacterial isolates from the roots of sugarcane were compared morphologically, biochemically and genetically. Gram staining, colony pigment, texture and other cultural characteristics were taken for morphological characterization. Oxidation-fermentation tests for D-glucose and D-sucrose, production of acid and hydrogen from different carbon source, oxidase activity, antibiotic and drug resistance patterns were chosen as the biochemical and physiological criteria. Twelve
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Research Interests: Chemistry, Analytical Chemistry, Chromatography, Pharmacognosy, Magnetic Resonance Spectroscopy, and 15 moreMedicine, Quantitative analysis, Separation Science, Calibration, Lignans, Chemical Analysis, High Pressure Liquid Chromatography, Phyllanthus, Quantitative Analysis, Densitometry, Quntitative Thin Layer Chromatography, Molecular Structure, Enantiomer, Plant Leaf, and stereoisomerism
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Summary In patients with reactive arthritis (ReA)/undifferentiated spondyloarthropathy (uSpA), synovial fluid mononuclear cells (SFMC) show proliferation to bacterial antigens that trigger ReA, i.e. Chlamydia, Yersinia, Campylobactor,... more
Summary In patients with reactive arthritis (ReA)/undifferentiated spondyloarthropathy (uSpA), synovial fluid mononuclear cells (SFMC) show proliferation to bacterial antigens that trigger ReA, i.e. Chlamydia, Yersinia, Campylobactor, Shigella and Salmonella species. We have shown previously that SFMC proliferate significantly to outer membrane proteins of S typhimurium in Salmonella induced ReA. In the present study we characterized the immunoreactive fractions of outer membrane protein (Omp) of S typhimurium in Salmonella induced ReA. Omp of Salmonella was isolated and fractionated by continuous elution sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) using Prep-Cell into eight Omp fractions based on molecular weight. Twenty-three patients with ReA were screened for the bacterial trigger using the SFMC proliferative response to crude lysates of Y enterocolitica, S flexneri, C jejuni and S typhimurium using thymidine uptake assay. SFMC from patients with salmon...
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Research Interests: Engineering, Genetics, Physics, Chemistry, Molecular Biology, and 15 moreBiotechnology, Plant Biology, Biology, Infectious Diseases, Medicine, Multidisciplinary, Biological Sciences, Expressed Sequence Tags, PLoS one, Genes, Antimalarials, Artemisia Annua, Artemisinin, PLANT PROTEINS, and Plant Leaves
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Research Interests:
The highly polymorphic human alpha-1 antitrypsin (AAT) gene codes for the most abundant circulating plasma serine protease inhibitor. Previously, genetic variants of the AAT gene were reported from different regions of the world. In the... more
The highly polymorphic human alpha-1 antitrypsin (AAT) gene codes for the most abundant circulating plasma serine protease inhibitor. Previously, genetic variants of the AAT gene were reported from different regions of the world. In the present study, the AAT gene was characterized in an Indian sample. The AAT gene was isolated and cloned from a liver biopsy sample through RT-PCR and the full-length gene was sequenced. Nucleotide sequence comparison with the human genome and the AAT sequences available in the GenBank (NCBI) demonstrated four unique variations--(i) an A to G variation at position 286 (Thr96Ala), (ii) an A to G variation at position 839 (Asp280Gly), (iii) a T to C variation at position 1182 that did not result in any change in the protein sequence (TTT to TTC both code for Phe) and (iv) an A to C variation at position 1200 (Glu400Asp) that resulted in replacement by an amino acid of similar nature. Other variations found were T to C at position 710 (Val273Ala) and T t...
Research Interests: Biology, Medicine, Biopsy, India, Humans, and 14 moreLiver, Indian, Human Genome, Genetic Polymorphism, Genetic variation, RT PCR, Codon, Amino Acid Sequence, Base Sequence, Alpha-1 Antitrypsin, Biochemistry and cell biology, Molecular Sequence Data, alleles, and reverse transcriptase polymerase chain reaction
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ABSTRACT The present invention relates to novel loganin analogues and a process for the preparation thereof. More particularly the present invention relates to use of Iridoid glycoside loganin isolated from the fruit pulp of Strychnos... more
ABSTRACT The present invention relates to novel loganin analogues and a process for the preparation thereof. More particularly the present invention relates to use of Iridoid glycoside loganin isolated from the fruit pulp of Strychnos nux-vomica and its bioactive semi-synthetic analogues against various human cancer cell lines grown in-vitro.
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... Authors Fatima, S.; Farooqi, AHA; Kumar, R.; Kumar, TRS; Khanuja, SPS Journal article; Conference paper Journal of Medicinal and Aromatic Plant Sciences 2000 Vol. 22 No. 4a pp. ... To learn more about our products please visit our... more
... Authors Fatima, S.; Farooqi, AHA; Kumar, R.; Kumar, TRS; Khanuja, SPS Journal article; Conference paper Journal of Medicinal and Aromatic Plant Sciences 2000 Vol. 22 No. 4a pp. ... To learn more about our products please visit our website. ...