m.o. elShazly
Cairo University, Pathology, Faculty Member
<p>The effect of SDD, spirulina and their co administration on catalase activity in kidney tissue of all treated groups.</p
Research Interests: Biological Sciences, MDA, Spirulina platensis, Cr, SDD, and 5 moreMg, Tissue, Gp, PCNA, and Alteration
<p>The effect of SDD, spirulina and their co-administration on chromium contents in blood and kidney tissue.</p
Research Interests: Biological Sciences, MDA, Spirulina platensis, Cr, SDD, and 5 moreMg, Tissue, Gp, PCNA, and Alteration
<p>The effect of SDD, spirulina and their co-administration on body weight gain of all treated groups.</p
Research Interests: Biological Sciences, MDA, Spirulina platensis, Cr, SDD, and 5 moreMg, Tissue, Gp, PCNA, and Alteration
<p>Showing A) Swelling of the tubular lining epithelium with variable degrees of granular and vacuolar degeneration and single cell necrosis(H&E X400). B) Wide spread nuclear pyknosis among the tubular epithelium (H&E X400).... more
<p>Showing A) Swelling of the tubular lining epithelium with variable degrees of granular and vacuolar degeneration and single cell necrosis(H&E X400). B) Wide spread nuclear pyknosis among the tubular epithelium (H&E X400). C) Atrophy of the glomerular tufts with mesangeal necrosis (H&E X400). D) Thickening of the glomerular basement membrane (PAS X400). E) Interstitial nephritis, notice the congested blood vessels and focal mononuclear inflammatory infiltrates (H&E X400). F) Proliferative hyperplasia of the renal tubular epithelium that appeared basophilic with vesicular and basophilic nuclei (H&E X200).</p
Research Interests: Biological Sciences, MDA, Spirulina platensis, Cr, SDD, and 5 moreMg, Tissue, Gp, PCNA, and Alteration
<p>A, B) Kidney of chromium treated rat showing positivity for PCNA expression in the nuclei of the hyperplastic tubular epithelial cells (X400).</p
Research Interests: Biological Sciences, MDA, Spirulina platensis, Cr, SDD, and 5 moreMg, Tissue, Gp, PCNA, and Alteration
Carbendazim (CBZ) is one of the most common fungicides used to fight plant fungal diseases, otherwise, it leaves residue on fruits, vegetables, and soil that contaminate the environment, water, animal, and human causing serious health... more
Carbendazim (CBZ) is one of the most common fungicides used to fight plant fungal diseases, otherwise, it leaves residue on fruits, vegetables, and soil that contaminate the environment, water, animal, and human causing serious health problems. Several studies have reported the reproductive and endocrine pathological disorders induced by CBZ in several animal models, but little is known about its neurotoxicity. So that, the present study aimed to explain the possible mechanisms of CBZ induced neurotoxicity in rats. Sixty male Wistar rats were divided into 4 groups (n = 15). Group (1) received normal saline and was kept as the negative control group, whereas groups (2, 3, 4) received CBZ at 100, 300, 600 mg/kg b.wt respectively. All rats received the aforementioned materials daily via oral gavage. Brain tissue samples were collected at 7, 14, 28 days from the beginning of the experiment. CBZ induced oxidative stress damage manifested by increasing MDA levels and reducing the levels of TAC, GSH, CAT in some brain areas at 14 and 28 days. There were extensive neuropathological alterations in the cerebrum, hippocampus, and cerebellum with strong caspase-3, iNOS, Cox-2 protein expressions mainly in rats receiving 600 mg/kg CBZ at each time point. Moreover, upregulation of mRNA levels of NF-κB, TNF-α, IL-1B genes and downregulation of the transcript levels of both AchE and MAO genes were recorded in all CBZ receiving groups at 14 and 28 days especially those receiving 600 mg/kg CBZ. Our results concluded that CBZ induced dose- and time-dependent neurotoxicity via disturbance of oxidant/antioxidant balance and activation of NF-κB signaling pathway. We recommend reducing the uses of CBZ in agricultural and veterinary fields or finding other novel formulations to reduce its toxicity on non-target organisms and enhance its efficacy on the target organisms.
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This study was conducted to investigate the possible protective effect of Spirulina platensis against chromium-induced nephrotoxicity. A total of 36 adult male Sprague-Dawley rats were divided into 4 equal groups (Gps). Gp1 served as... more
This study was conducted to investigate the possible protective effect of Spirulina platensis against chromium-induced nephrotoxicity. A total of 36 adult male Sprague-Dawley rats were divided into 4 equal groups (Gps). Gp1 served as control, rats of Gps 2, 3, and 4 were exposed to Spirulina platensis (300 mg/kg b.wt per os) and sodium dichromate dihydrate (SDD) via drinking water at concentration of 520 mg /l respectively. Chromium administra-tion caused alterations in the renal function markers as evidenced by significant increase of blood urea and creatinine levels accompanied with significant increase in kidney’s chromi-um residues and MDA level as well as decreased catalase activity and glutathion content in kidney tissue. Histologically, Cr provoked deleterious changes including: vascular conges-tion, wide spread tubular epithelium necrobiotic changes, atrophy of glomerular tuft and pro-liferative hyperplasia. The latter was accompanied with positive PCNA expression in kidney ...
<p>The effect of SDD, spirulina and their co administration on serum urea and creatinine levels of all treated groups.</p
Research Interests: Biological Sciences, MDA, Spirulina platensis, Cr, SDD, and 5 moreMg, Tissue, Gp, PCNA, and Alteration
<p>The effect of SDD, spirulina and their co administration on MDA and GSH contents in kidney tissue of all treated groups.</p
Research Interests: Biological Sciences, MDA, Spirulina platensis, Cr, SDD, and 5 moreMg, Tissue, Gp, PCNA, and Alteration
<p>A, B) Kidney of chromium treated rat showing positivity for PCNA expression in the nuclei of the hyperplastic tubular epithelial cells (X400).</p
Research Interests: Biological Sciences, MDA, Spirulina platensis, Cr, SDD, and 5 moreMg, Tissue, Gp, PCNA, and Alteration
Carbendazim (CBZ) is one of the most common fungicides used to fight plant fungal diseases, otherwise, it leaves residue on fruits, vegetables, and soil that contaminate the environment, water, animal, and human causing serious health... more
Carbendazim (CBZ) is one of the most common fungicides used to fight plant fungal diseases, otherwise, it leaves residue on fruits, vegetables, and soil that contaminate the environment, water, animal, and human causing serious health problems. Several studies have reported the reproductive and endocrine pathological disorders induced by CBZ in several animal models, but little is known about its neurotoxicity. So that, the present study aimed to explain the possible mechanisms of CBZ induced neurotoxicity in rats. Sixty male Wistar rats were divided into 4 groups (n = 15). Group (1) received normal saline and was kept as the negative control group, whereas groups (2, 3, 4) received CBZ at 100, 300, 600 mg/kg b.wt respectively. All rats received the aforementioned materials daily via oral gavage. Brain tissue samples were collected at 7, 14, 28 days from the beginning of the experiment. CBZ induced oxidative stress damage manifested by increasing MDA levels and reducing the levels of TAC, GSH, CAT in some brain areas at 14 and 28 days. There were extensive neuropathological alterations in the cerebrum, hippocampus, and cerebellum with strong caspase-3, iNOS, Cox-2 protein expressions mainly in rats receiving 600 mg/kg CBZ at each time point. Moreover, upregulation of mRNA levels of NF-κB, TNF-α, IL-1B genes and downregulation of the transcript levels of both AchE and MAO genes were recorded in all CBZ receiving groups at 14 and 28 days especially those receiving 600 mg/kg CBZ. Our results concluded that CBZ induced dose- and time-dependent neurotoxicity via disturbance of oxidant/antioxidant balance and activation of NF-κB signaling pathway. We recommend reducing the uses of CBZ in agricultural and veterinary fields or finding other novel formulations to reduce its toxicity on non-target organisms and enhance its efficacy on the target organisms.
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This study was conducted to investigate the possible protective effect of Spirulina platensis against chromium-induced nephrotoxicity. A total of 36 adult male Sprague-Dawley rats were divided into 4 equal groups (Gps). Gp1 served as... more
This study was conducted to investigate the possible protective effect of Spirulina platensis against chromium-induced nephrotoxicity. A total of 36 adult male Sprague-Dawley rats were divided into 4 equal groups (Gps). Gp1 served as control, rats of Gps 2, 3, and 4 were exposed to Spirulina platensis (300 mg/kg b.wt per os) and sodium dichromate dihydrate (SDD) via drinking water at concentration of 520 mg /l respectively. Chromium administra-tion caused alterations in the renal function markers as evidenced by significant increase of blood urea and creatinine levels accompanied with significant increase in kidney’s chromi-um residues and MDA level as well as decreased catalase activity and glutathion content in kidney tissue. Histologically, Cr provoked deleterious changes including: vascular conges-tion, wide spread tubular epithelium necrobiotic changes, atrophy of glomerular tuft and pro-liferative hyperplasia. The latter was accompanied with positive PCNA expression in kidney ...
This study was conducted to investigate the possible protective effect of Spirulina platensis against chromium-induced nephrotoxicity. A total of 36 adult male Sprague-Dawley rats were divided into 4 equal groups (Gps). Gp1 served as... more
This study was conducted to investigate the possible protective effect of Spirulina platensis against chromium-induced nephrotoxicity. A total of 36 adult male Sprague-Dawley rats were divided into 4 equal groups (Gps). Gp1 served as control, rats of Gps 2, 3, and 4 were exposed to Spirulina platensis (300 mg/kg b.wt per os) and sodium dichromate dihydrate (SDD) via drinking water at concentration of 520 mg /l respectively. Chromium administration caused alterations in the renal function markers as evidenced by significant increase of blood urea and creatinine levels accompanied with significant increase in kidney's chromium residues and MDA level as well as decreased catalase activity and glutathion content in kidney tissue. Histologically, Cr provoked deleterious changes including: vascular congestion, wide spread tubular epithelium necrobiotic changes, atrophy of glomerular tuft and proliferative hyperplasia. The latter was accompanied with positive PCNA expression in kidney ...
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<p>Showing A, B) Scattered foci of hyperplastic tubules with thick basement membrane (H&E and PAS X400). C, D) The hyperplastic cells partially lined the tubular circumference or away around the tubule insinuating in the... more
<p>Showing A, B) Scattered foci of hyperplastic tubules with thick basement membrane (H&E and PAS X400). C, D) The hyperplastic cells partially lined the tubular circumference or away around the tubule insinuating in the intertubular areas as rows (thick arrow) or clumps (arrow) or forming a tubular pattern (H&E X400). E) Mononuclear inflammatory cells infiltration accompanying the hyperplastic tubules with minimal increased number of mesenchymal interstitial cells (H&E X400). F) Kidney of chromium and spirulina co-treated rats showing wide spread foci of regenerative tubules (H&E X200).</p
Research Interests: Biological Sciences, MDA, Spirulina platensis, Cr, SDD, and 5 moreMg, Tissue, Gp, PCNA, and Alteration
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The mitigative effect of Raphanus sativus oil on chromium-induced geno-and hepatotoxicity in male Rats, Journal of Advanced Research (2016), doi: http://dx.
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This study was conducted to investigate the possible protective effect of Spirulina platensis against chromium-induced nephrotoxicity. A total of 36 adult male Sprague-Dawley rats were divided into 4 equal groups (Gps). Gp1 served as... more
This study was conducted to investigate the possible protective effect of Spirulina platensis
against chromium-induced nephrotoxicity. A total of 36 adult male Sprague-Dawley rats
were divided into 4 equal groups (Gps). Gp1 served as control, rats of Gps 2, 3, and 4 were
exposed to Spirulina platensis (300 mg/kg b.wt per os) and sodium dichromate dihydrate
(SDD) via drinking water at concentration of 520 mg /l respectively. Chromium administration
caused alterations in the renal function markers as evidenced by significant increase of
blood urea and creatinine levels accompanied with significant increase in kidney’s chromium
residues and MDA level as well as decreased catalase activity and glutathion content in
kidney tissue. Histologically, Cr provoked deleterious changes including: vascular congestion,
wide spread tubular epithelium necrobiotic changes, atrophy of glomerular tuft and proliferative
hyperplasia. The latter was accompanied with positive PCNA expression in kidney
tissues as well as DNA ploidy interpretation of major cellular population of degenerated
cells, appearance of tetraploid cells, high proliferation index and high DNA index. Morphometrical
measurements revealed marked glomerular and tubular lumen alterations. On contrary,
spirulina co-treatment with Cr significantly restored the histopathological changes,
antioxidants and renal function markers and all the previously mentioned changes as well.
against chromium-induced nephrotoxicity. A total of 36 adult male Sprague-Dawley rats
were divided into 4 equal groups (Gps). Gp1 served as control, rats of Gps 2, 3, and 4 were
exposed to Spirulina platensis (300 mg/kg b.wt per os) and sodium dichromate dihydrate
(SDD) via drinking water at concentration of 520 mg /l respectively. Chromium administration
caused alterations in the renal function markers as evidenced by significant increase of
blood urea and creatinine levels accompanied with significant increase in kidney’s chromium
residues and MDA level as well as decreased catalase activity and glutathion content in
kidney tissue. Histologically, Cr provoked deleterious changes including: vascular congestion,
wide spread tubular epithelium necrobiotic changes, atrophy of glomerular tuft and proliferative
hyperplasia. The latter was accompanied with positive PCNA expression in kidney
tissues as well as DNA ploidy interpretation of major cellular population of degenerated
cells, appearance of tetraploid cells, high proliferation index and high DNA index. Morphometrical
measurements revealed marked glomerular and tubular lumen alterations. On contrary,
spirulina co-treatment with Cr significantly restored the histopathological changes,
antioxidants and renal function markers and all the previously mentioned changes as well.
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This study was conducted to investigate the Pathological, Immunohistochemical and Biochemical effects of Radish Oil on Streptozotocin induced diabetic male rats. Eighty Albino rats (weighing about 250-300 g) were used in this study, they... more
This study was conducted to investigate the Pathological, Immunohistochemical and Biochemical effects of Radish Oil on Streptozotocin induced diabetic male rats. Eighty Albino rats (weighing about 250-300 g) were used in this study, they were randomly divided into 4 equal groups, each containing 20 rats as follows, group 1; control negative rats, group 2; rats administrated radish oil (300 mg/kg.b.w. orally); group 3: STZ diabetic rats (single intra-peritoneal injection of streptozotocin (STZ, 60 mg/kg) and group 4: STZ diabetic rats treated with radish oil (300 mg/kg.b.w) . Blood samples and tissue specimens were collected from pancreas, liver and kidneys from all rats of all groups at 15, 30 and 45 days from the start of the experiment for biochemical and histopathological examinations respectively. The biochemical analysis revealed significant increase in lipase, α-amylase, AST, ALT, ALP, urea and creatinine levels in the serum of diabetic rats, while the diabetic rat treated with radish oil showed a significant amelioration in those previously mentioned parameters. The histopathological examination revealed alterations in the pancreas of diabetic rats characterized by necrosis and atrophy of β-cells of islets of Langerhans. Activation of kupffer cells with cytoplasmic vacuolation of hepatocytes, vacuolation and necrosis of renal tubular epithelium were also noticed. However, regeneration in the pancreas, liver and kidneys was observed in diabetic rats treated with radish oil. In Pancreas of diabetic rats, the immunoreactivity for anti-insulin antibodies was markedly decreased (decrease in the number of insulin positive cells). However, after treatment with radish oil the positive immunoreactions of B-cells for antiinsulin antibodies were obviously increased. From this study we could conclude that Raphanus sativus oil contains many antioxidant compounds that stimulate regeneration and reactivation of B- cells to produce more insulin and this improvement was confirmed by biochemical parameters, histopathological findings in the pancreas, liver and kidneys as well as the immunohistochemistry of anti-insulin antibodies in the pancreatic tissue.
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This study aimed to evaluate the therapeutic effects of Spirulina Platensis on streptozotocin-induced diabetic Rats. The present experimental study was carried out on 80 Albino rats (weighing about 250-300 g) divided randomly into 4 equal... more
This study aimed to evaluate the therapeutic effects of Spirulina Platensis on streptozotocin-induced diabetic Rats. The present experimental study was carried out on 80 Albino rats (weighing about 250-300 g) divided randomly into 4 equal groups, 20 rats each. Group 1; served as control , group 2: spirulina treated rats (200 mg/kg.b.w), group 3; STZ diabetic rats (single intra-peritoneal injection of streptozotocin (STZ, 60 mg/kg) and group 4; diabetic rats treated with spirulina (200 mg/kg.b.w) . Serum samples and tissue specimens were collected from pancreas, liver and kidneys at 15, 30 and 45 days post experiment for biochemical and histopathological examinations respectively. The biochemical results revealed significant increase in lipase, α-amylase , AST , ALT , ALP , urea and creatinine levels in the serum of diabetic rats, whereas, the diabetic rat treated with Spirulina showed decrease in those biochemical parameters. The histopathological alterations observed in the pancreas of diabetic rats were vacuolation of epithelial lining pancreatic acini, necrosis and atrophy of β-cells of islets of Langerhans. Concerning liver of diabetic rats, it showed dilatation and congestion of hepatic sinusoids and activation of kupffer cells with dissociation of hepatic cords and cytoplasmic vacuolation of hepatocytes. The renal parenchyma of diabetic rats showed marked congestion of glomerular tuft and intertubular blood capillaries with necrobiotic changes and necrosis of renal tubular epithelium associated with focal mononuclear cell infiltration. Marked improvement in the histopathological picture was noticed in the pancreas, liver and kidneys of diabetic rats treated with Spirulina platensis. From the observed results we could concluded that Spirulina platensis contains many antioxidant compounds that could stimulate regeneration and reactivation of B- cells to produce more insulin and this improvement was confirmed by the biochemical parameters and histopathological findings in the pancreas, liver and kidneys.
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Eighteen local breed cats suffered from enterotoxaemia after scavenging on chicken-remnants. They suffered from vomitus and diarrhoea and collapsed dead after a few hours. An untypable strain of Clostridium perfringens was isolated in... more
Eighteen local breed cats suffered from enterotoxaemia after scavenging on chicken-remnants. They suffered from vomitus and diarrhoea and collapsed dead after a few hours. An untypable strain of Clostridium perfringens was isolated in pure form and was suspected to be the cause of the outbreak
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Chicken anaemia virus (CAV) was identified in the bone marrow of birds in 9 flocks (total 200 000 birds) on a multiple-age farm in Saudi Arabia during the first half of 1994. Mortality was 7% among young birds but had declined to normal... more
Chicken anaemia virus (CAV) was identified in the bone marrow of birds in 9 flocks (total 200 000 birds) on a multiple-age farm in Saudi Arabia during the first half of 1994. Mortality was 7% among young birds but had declined to normal by 2 weeks of age. About 25% of the birds failed to reach the expected body weight by 7 weeks. The affected groups were of unequal size and over 20% of carcasses were rejected after slaughter for muscular haemorrhages in breasts and thighs. The disease was reproduced in CAV-free birds by i.m. inoculation of a liver extract from naturally infected birds.
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Immunoassays showed that the immunomodulator, Nutrilac, strongly sustained the production of anti-sheep red blood cells (SRBCs) haemagglutinating (HA) antibodies from 7 days after inoculation, with a geometric mean ranging between... more
Immunoassays showed that the immunomodulator, Nutrilac, strongly sustained the production of anti-sheep red blood cells (SRBCs) haemagglutinating (HA) antibodies from 7 days after inoculation, with a geometric mean ranging between 2.1-5.5, in cyclophosphamide (CP)-immunosuppressed chicks. Vitamin E was less active sustaining an HA geometric mean titre at 14 and 21 days after inoculation of only 0.5 for each. Administration of Nutrilac and vitamin E markedly improved the level of serum transferrin, IgG and IgM during the whole experimental period. They also significantly increased stimulation indices of lymphocyte transformation 7-14 days after inoculation with SRBCs for the 21-day experimental period; Nutrilac was superior to vitamin E. Bursal/body weight index was significantly increased at 14 and 21 days after treatment with Nutrilac and at 21 days with vitamin E compared with CP-immunosuppressed non-treated controls. Histomorphological features of bursa fabricii, thymus gland and spleen were markedly improved with Nutrilac and vitamin E from day 7 after treatment. The improvement was more obvious in Nutrilac-treated chickens. Protection percentage against Escherichia coli challenge, measured by bioassay, reached 80% and 68.9% in CP-immunosuppressed chicks immunopotentiated with Nutrilac or vitamin E, respectively, as compared with 48.9% in immunosuppressed non-immunopotentiated birds