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Abhay K Pandey
  • Hyderabad, Andhra Pradesh, India
  • Dr. Abhay K. Pandey is a Mycologist at the Tocklai Tea Research Institute, India, exploring microbial biocontrol agen... moreedit
  • Prof. Uma T Palni & Prof. N N Tripathiedit
In the rice-fallow system, dry root rot (DRR) is an emerging disease of mungbean (Vigna radiata (L.) R. Wilczek var. radiata) caused by the necrotrophic fungus Macrophomina phaseolina. The pathogen causes extensive production losses. In... more
In the rice-fallow system, dry root rot (DRR) is an emerging disease of mungbean (Vigna radiata (L.) R. Wilczek var. radiata) caused by the necrotrophic fungus Macrophomina phaseolina. The pathogen causes extensive production losses. In this study, the bioactivity of four Trichoderma harzianum isolates, namely Th-Dharwad, Th-Raichur, Th-Niphm, and Th-Udaipur procured from the Indian research institutes were evaluated against M. phaseolina of mungbean by the dual culture technique. The efficacy of these T. harzianum isolates were also compared with the effective fungicides such as thiram and carbendazim by the poison food method. Results showed that among the T. harzianum isolates, isolate of Th-Raichur was most effective, exhibiting 76.96% mycelial growth inhibition of the test pathogen. As compared to the thiram, carbendazim was more effective, and exhibited 100% mycelial growth inhibition of the test pathogen. In addition, carbendazim was also more effective than the isolate of Th...
The objective of the present study is to investigate the effect of physical factors on mycotoxic potency of Chenopodium ambrosioides Linn. and Clausena pentaphylla (Roxb.) DC oils against four species of Aspergilli such as A. flavus Link,... more
The objective of the present study is to investigate the effect of physical factors on mycotoxic potency of Chenopodium ambrosioides Linn. and Clausena pentaphylla (Roxb.) DC oils against four species of Aspergilli such as A. flavus Link, A. niger van Tieghem, A. ochraceus Wilhelm, A. terreus Thom causing post-harvest deterioration of pigeon pea seeds. During experimentation it was observed that the potency of both oils remained unchanged even against heavy inoculum dose (maximum number of 10 fungal discs and diameter of 25 mm). Furthermore, the toxicity of both the oils did not alter even up to 120 ºC of temperature and 12 months of storage by exhibiting 100% mycelial inhibition of test fungi.  The toxicity of both the oils was decreased at alkaline pH (7, 8).  Physicochemical characterization of oils revealed that C. ambrosioides oil was pale yellow in colour, lighter than water, laevorotatory, acidic in nature and showed positive test for phenols. While the oil of C. pentaphyllah...
is a genus from the family Lamiaceae, whose essential oils has long been used in various forms such as in management of plant pathogens and insect pests, in traditional medicine as well as in culinary and cosmetics. Its major chemical... more
is a genus from the family Lamiaceae, whose essential oils has long been used in various forms such as in management of plant pathogens and insect pests, in traditional medicine as well as in culinary and cosmetics. Its major chemical components such as menthol, carvone have now been successfully commercialized in the industry as antimicrobials/insecticidal agents. Current review focuses on chemical composition of essential oils of some species from different geographical regions with their insecticidal (repellent, antifeedant, and ovicidal) and antimicrobial efficacies against bacterial, fungal plant pathogens and insects of stored products. Reports of the researchers on chemical analysis of essential oils of species revealed that most of the oils being rich in pulegone, menthon, menthol, carvone, 1, 8-cineole, limonene and β-caryophyllene. Reviewed literature revealed that, essential oils from different species possess potential antimicrobial activity against plant pathogens and h...
Salmonella enterica is a well-known pathogenic bacterium that can cause intracellular illness and food poisoning in humans, especially salmonellosis. In the present study, 20 essential oils of aromatic plants were screened for their... more
Salmonella enterica is a well-known pathogenic bacterium that can cause intracellular illness and food poisoning in humans, especially salmonellosis. In the present study, 20 essential oils of aromatic plants were screened for their antibacterial activity against S. enterica. Among the essential oils screened, Citrus aurantifolia essential oil (CAEO) demonstrated most efficient activity against S. enterica. CAEO had minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 8 and 16 μl/mL, respectively. A significant scavenging of radical cations was observed with CAEO using 2, 2'-diphenyl-1-picryl hydrazyl with an IC50 of 0.2147 mg/mL. Total phenolic content of CAEO was 10.90 mg GAE/100 μL. The treatment of bacterium with CAEO caused the leakage of cellular components, i.e., both nucleic acids and proteins. Furthermore, scanning electron microscopy (SEM) was used to measure the ultrastructure of control and treated bacterium (due to CAEO). The result...
Cowpea [Vigna unguiculata L. (Walp.)], is an important legume crop widely grown in the tropics. Biotic and abiotic stresses cause significant yield reduction in cowpea. In this chapter, we provide a synthesis of information on the... more
Cowpea [Vigna unguiculata L. (Walp.)], is an important legume crop widely grown in the tropics. Biotic and abiotic stresses cause significant yield reduction in cowpea. In this chapter, we provide a synthesis of information on the damage/economic importance of soilborne diseases of cowpea and present options that can be used to manage these diseases. The aim is to demonstrate that a wide array of control options are available for potential use within an integrated disease management (IDM) framework. Reviewed literature indicated presence of several sources of resistance to fusarium wilt (FW) and charcoal rot but few sources for stem rots, collar rot and damping-off. Major resistant genes and quantitative trait loci (QTL) were identified for FW and charcoal rot and these may be exploited in marker assisted selection (MAS). Cultural practices such as crop rotation and compositing were found to be effective against soilborne diseases, however, there is lack of knowledge regarding their...
The present paper deals with survey and documentation of aromatic plants of Gorakhpur division in relation to their flowering/fruiting periods, antimycotic property and medicinal value. During study various places of Gorakhpur division... more
The present paper deals with survey and documentation of aromatic plants of Gorakhpur division in relation to their flowering/fruiting periods, antimycotic property and medicinal value. During study various places of Gorakhpur division were surveyed periodically from 2006-2009. Forty two aromatic plant species belonging to 32 genera and 15 families were collected and identified. Out of 15 families Asteraceae was dominant having more aromatic plants followed by Lamiaceae and Rutaceae, while Acanthaceae, Apiaceae, Caesalpiniaceae, Euphorbiaceae, Liliaceae and Lythraceae exhibited lower number of plant species. Flowering and fruiting in maximum aromatic plant species occurred during January–April. The collected aromatic plants were evaluated for their antimycotic activity against Aspergillus flavus, A. niger, A. ochraceus and A. terreus. Besides, medicinal importance of plants was also added.
Dry root rot (DRR) is an important emerging disease of mungbean caused by fungus Macrophomina phaseolina. The disease is seed and soil-borne and hence management is difficult. Exploitation of host resistance could be a good option to... more
Dry root rot (DRR) is an important emerging disease of mungbean caused by fungus Macrophomina phaseolina. The disease is seed and soil-borne and hence management is difficult. Exploitation of host resistance could be a good option to manage the disease. The objectives of this study were to characterize the isolates of M. phaseolina from three legume crops and to identify resistant sources against DRR of mungbean. Isolates of M. phaseolina from mungbean, urdbean, and vegetable soybean were identified using morphological characteristics and sequencing internal transcribed sequence (ITS) region of 18S rRNA. Isolates of M. phaseolina from urdbean and vegetable soybean were inoculated on susceptible check genotype of mungbean to assess their pathogenicity. Forty three mungbean genotypes were screened against M. phaseolina isolate of mungbean using the paper towel method to identify sources of resistance. Among these genotypes, 9 were resistant in repeated experiment, with disease score r...
Eight fungal species namely Aspergillus flavus A. nidulans, A. niger, A. ochraceus, Mucor sp., Penicillium italicum, P. oxalicum and Rhizopus arrhizus were identified by agar plate as well as blotter paper methods from fresh kernels of... more
Eight fungal species namely Aspergillus flavus A. nidulans, A. niger, A. ochraceus, Mucor sp., Penicillium italicum, P. oxalicum and Rhizopus arrhizus were identified by agar plate as well as blotter paper methods from fresh kernels of Buchnania lanzan. in vitro activity of 12 essential oils and 2 commercial fungicides was assessed for their antifungal activity against all isolated fungi. Oil of Ocimum canum exhibited absolute toxicity (100%) against 8 fungi at 500ppm concentration. in vivo studies depict that oil of O. canum as seed dressing agent and as a fumigant was able to preserve kernels up to 45 and 90 days, respectively with minimal changes in organoleptic behaviour of fruits during storage. Thus, Ocimum oil can possibly be exploited in the management of seed-borne pathogenic fungi and prevention of biodeterioration of Buchnania kernels in an eco-friendly way.
From time immemorial, man has been in a continual fight with pests (Insects, plant disease, rodents, weeds etc.) that compete for his food supply, damage his forests and live stocks and is responsible for several diseases. The interest in... more
From time immemorial, man has been in a continual fight with pests (Insects, plant disease, rodents, weeds etc.) that compete for his food supply, damage his forests and live stocks and is responsible for several diseases. The interest in the use of essential oils for the pests and pathogens control is not new and originates from the need for pesticide production with eco-friendly, easily biodegradability and negligible health impacts than synthetic pesticides. These oils have a potential to be used as protectant of stored products including cereals, grains and pulses. Recently it has been reported that some chemical constituents derived from such essential oils also affect the nervous system of stored product insects. The expanding literature on the possibility of the use of these volatiles is reviewed in the present time. It focuses on the oil's effect on oviposition, repellency, ovicidal, pupicidal, larvicidal, insecticidal and antifeedant activities of insects. Possible attr...
Powdery mildew is a significant threat to mungbean (Vigna radiata) and black gram (V. mungo) production across Australia and overseas. Although they have been present in Australia for at least six decades and are easily recognized in the... more
Powdery mildew is a significant threat to mungbean (Vigna radiata) and black gram (V. mungo) production across Australia and overseas. Although they have been present in Australia for at least six decades and are easily recognized in the field, the precise identification of the pathogens causing this disease has remained unclear. Our goal was to identify the powdery mildew species infecting mungbean, black gram, and wild mungbean (V. radiata ssp. sublobata) in Australia. The internal transcribed spacer (ITS) and large subunit sequences of the ribosomal DNA and/or morphology of 57 Australian specimens were examined. Mungbean and black gram were infected by two species: Podosphaera xanthii and a newly recognized taxon, Erysiphe vignae sp. nov. Wild mungbean was infected only with P. xanthii. Mungbean and black gram powdery mildew ITS sequences from China, India, and Taiwan revealed the presence of only P. xanthii on these crops despite controversial reports of an Erysiphe species on b...
The damage to crops and food commodities by pests and diseases remains a major public health concern. Globally, investigations were carried out to develop harmless natural products from essential oils (EOs) and advances were made to meet... more
The damage to crops and food commodities by pests and diseases remains a major public health concern. Globally, investigations were carried out to develop harmless natural products from essential oils (EOs) and advances were made to meet users’ acceptance as an alternative to chemical fungicides and insecticides. Recently, many EOs have shown potential results against a wide range of plant pathogens and insect pests, and are thus, attractive substitutes to synthetic chemicals. Remarkably, EOs based commercial pesticides remain lesser in number and this opens several prospects for their claim for use in present agriculture. The present paper provides a summary of the current status and prospects in evaluating the efficiency of the use of EO of Dysphania ambrosioides (L.) Mosyakin & Clemants syn. Chenopodium ambrosioides L. (Mexican tea) belonging to the family Amaranthaceae, in crop protection strategies against plant pathogens and insect pests, considering the opportunities and problems linked with the prospects of sustainable environment-friendly agriculture in the future. In addition, the chemical composition of D. ambrosioides EO from different geographical regions along with the pharmacological application, probable modes of action, and indication for or against the use of this EO as an alternative to chemicals, in several applications have been discussed. The EO of D. ambrosioides possessed potential pharmacological activities, such as antitumor, antiprotozoal, allelopathic, antiviral, immunoregulatory, anti-inflammatory, and antioxidant activities. Most of the chemically characterized EO of D. ambrosioides contained ascaridole, α-terpinene, p-cymene, and caryophyllene oxide as major components, and have shown efficient antibacterial, antifungal, and insecticidal activities against pests and pathogens of economically important crops. Thus, the EO of D. ambrosioides and its major constituents can be utilized as potential botanical pesticides for crop protection after large-scale evaluation.
In this study, repellent and fumigant activities of Tanacetum tomentosum and Ta. dolichophyllum essential oils were investigated against Tribolium castaneum. Results showed that both oils had effective fumigant and repellent activities.... more
In this study, repellent and fumigant activities of Tanacetum tomentosum and Ta. dolichophyllum essential oils were investigated against Tribolium castaneum. Results showed that both oils had effective fumigant and repellent activities. Tanacetum tomentosum oil emerged as more potent than Ta. dolichophyllum oil with LC50 values of 6.85 and 4.32 μL/0.25 L air after 24 and 48 h of exposure, respectively. Ta. tomentosum oil also showed more repellent activity than Ta. dolichophyllum oil by exhibiting repellent activity in the range of 38.70–82.35%. Chemical analysis of both oils revealed that β‐bisabolene (50.0%) was the major component of Ta. tomentosum oil and that β‐eudesmol (31.4%) and α‐bisabolol (10.7%) were the major components of Ta. dolichophyllum oil. Doses of 40 and 80 μL/0.5 L air of both oils significantly protected 500 g of wheat grains for up to 6 months from insect infestation, without causing any side effects in the germination rates; the order of germination rates was Ta. tomentosum (89.45%) > Ta. dolichophyllum (86.78%). Thus, this study suggests that essential oils from Ta. tomentosum and Ta. dolichophyllum can be used as potential alternative botanical fumigants for controlling Tr. castaneum.
ABSTRACT Thirty essential oils from higher plants of Gorakhpur Division (India) were evaluated at 0.36 μl/ml against two pulse beetles, Callosobruchus chinensis L. and C. maculatus F., causing infestation of pigeon pea seeds during... more
ABSTRACT Thirty essential oils from higher plants of Gorakhpur Division (India) were evaluated at 0.36 μl/ml against two pulse beetles, Callosobruchus chinensis L. and C. maculatus F., causing infestation of pigeon pea seeds during storage. Clausena pentaphylla oil was more effective and exhibited absolute repellency against both the insects followed by Ocimum canum, Salvia plebeia and Zingiber zerumbet oils. Among these four oils, C. pentaphylla oil was most toxic and showed 100% mortality of both the insects at 10-μl dosage and 24-h exposure (LD50 = 2.7 μl for C. chinensis & 2.4 μl for C. maculatus). Physical factors, viz. temperature, storage and autoclaving, did not cause any adverse effect on the toxicity of Clausena oil. During in vivo investigation, the oil protected 1 kg of pigeon pea seeds completely without reducing weight loss and seed damage up to 6 months when stored in gunny bags and glass containers. The oil was standardised by determining its various physicochemical properties. Thus, C. pentaphylla oil can be judiciously exploited as herbal insecticide against pulse beetles of pigeon pea seeds during storage.
In vitro antibacterial activities of the essential oils of aromatic plants against Erwinia herbicola (Lohnis) and
During mycofloral analysis of green gram (Vigna radiata (L.) R. Wilczek) seed samples taken from different grocery stores by agar and standard blotter paper methods, 5 fungal species were identified, of which Aspergillus flavus exhibited... more
During mycofloral analysis of green gram (Vigna radiata (L.) R. Wilczek) seed samples taken from different grocery stores by agar and standard blotter paper methods, 5 fungal species were identified, of which Aspergillus flavus exhibited higher relative frequency (75.20% to 80.60%) and was found to produce aflatoxin B1 . On screening of 11 plant essential oils against this mycotoxigenic fungi, Lippia alba essential oil was found to be most effective and showed absolute inhibition of mycelia growth at 0.28 μL/mL. The oil of L. alba was fungistatic and fungicidal at 0.14 and 0.28 μL/mL, respectively. Oil had broad range of fungitoxicity at its MIC value and was absolutely inhibited the AFB1 production level at 2.0 μL/mL. Chemical analysis of this oil revealed geranial (36.9%) and neral (29.3%) as major components followed by myrcene (18.6%). Application of a dose of 80 μL/0.25 L air of Lippia oil in the storage system significantly inhibited the fungal proliferation and aflatoxin prod...
ABSTRACT Basidiocarps of Ganoderma lucidum (Leyss: Fr) Karst are of immense medicinal value with anticancerous and immunomodulating properties. During the present investigation, stored basidiocarps revealed presence of nine deteriorating... more
ABSTRACT Basidiocarps of Ganoderma lucidum (Leyss: Fr) Karst are of immense medicinal value with anticancerous and immunomodulating properties. During the present investigation, stored basidiocarps revealed presence of nine deteriorating fungal species viz., Aspergillus carneus, A. flavipes, A. flavus, A. ochraceus, A. parasiticus, A. sulphurious, A. terreus, A. tubengensis and Fusarium nivale. Essential oils of 12 aromatic plant species and 3 chemical fungicides were evaluated for their antifungal potential against these fungal species. Chenopodium ambrosioides oil was found to be most potent. The oil exhibited greater toxicity than Bavistin during storage; the oil was able to preserve basidiocarps up to 15–25 days
In order to get a potent botanical fungicide for the management of fungal decay of table grapes, an experiment was conducted in which 20 essential oils of higher plants were screened at 0.33 µL mL(-1) against dominant fungi causing decay... more
In order to get a potent botanical fungicide for the management of fungal decay of table grapes, an experiment was conducted in which 20 essential oils of higher plants were screened at 0.33 µL mL(-1) against dominant fungi causing decay of table grapes, including Aspergillus flavus, A. niger and A. ochraceus. Furthermore, the minimum inhibitory/fungicidal concentration, fungitoxic spectrum and mycotoxin inhibition activity of the most potent oil were determined. The efficacy of the most potent oil in preservation of table grapes, along with organoleptic evaluation, was also carried out by storing 1 kg of grapes in the oil vapour. Artemisia nilagirica oil was found to be most toxic, exhibiting 100% mycelia inhibition of all test fungi. Moreover, 0.29 µL mL(-1) A. nilagirica oil was fungistatic and 0.58 µL mL(-1) was fungicidal for all tested species of Aspergillus. The oil exhibited a broad range of fungitoxicity against other grape berry-rotting fungi. Artemisia nilagirica oil completely suppressed the growth and mycotoxin (AFB1 and OTA) secretion of aflatoxigenic and ochratoxigenic strains of Aspergillus at 1.6 µL mL(-1) . During the in vivo experiment, fumigation of 1 kg of table grapes with 200 and 300 µL dosage of A. nilagirica oil enhanced the shelf life for up to 9 days. The oil did not show any phytotoxic effect. Besides, oil application did not substantively change the sensory properties of the fruits. Artemisia nilagirica oil can be used as an alternative botanical fungicide for the control of fruit-rotting fungi of stored grapes. © 2014 Society of Chemical Industry.
This study was designed to examine in vitro antibacterial activities of essential oils extracted from 53 aromatic plants of Gorakhpur Division (UP, INDIA) for the control of two phytopathogenic bacteria namely Erwinia herbicola and... more
This study was designed to examine in vitro antibacterial activities of essential oils extracted from 53 aromatic plants of Gorakhpur Division (UP, INDIA) for the control of two phytopathogenic bacteria namely Erwinia herbicola and Pseudomonas putida causing several post-harvest diseases in fruits and vegetables. Out of 53 oils screened, 8 oils such as Chenopodium ambrosioides, Citrus aurantium, Clausena pentaphylla, Hyptis suaveolens, Lippia alba, Mentha arvensis, Ocimum sanctum and Vitex negundo completely inhibited the growth of test bacteria. Furthermore MIC & MBC values of C. ambrosioides oil were least for Erw. herbicola (0.25 & 2.0 ?l/ml) and Ps. putida (0.12 & 1.0 ?l/ml) respectively than other 7 oils as well as Agromycin and Streptomycin drugs used in current study. GC and GC-MS analysis of Chenopodium oil revealed presence of 125 major and minor compounds, out of them, 14 compounds were recognized. The findings concluded that Chenopodium oil may be regarded as safe antibac...
Essential oils from 35 aromatic and medicinal plant species of Gorakhpur Division (U. P., India) were evaluated for their repellent activity against pulse bruchids Callosobruchus chinensis L. and C. maculatus F. of stored pigeon pea... more
Essential oils from 35 aromatic and medicinal plant species of Gorakhpur Division (U. P., India) were evaluated for their repellent activity against pulse bruchids Callosobruchus chinensis L. and C. maculatus F. of stored pigeon pea seeds. The oil concentration was at 0.36 μl/ml. Out of 35 essential oils, Adhatoda vasica Ness and Chenopodium ambrosioides L. oils showed absolute (100 %) insect repellency. Chenopodium oil exhibited 100 % mortality for both the test insects at 10 μl concentration (LD50 = 2.8 μl for C. chinensis & 2.5 μl for C. maculatus) and more toxic than Adhatoda oil (LD50 = 6.8 μl for C. chinensis & 8.4 μl for C. maculatus). During in vivo evaluation, 0.29 and 0.58 μl/ml of Chenopodium oil significantly enhanced feeding deterrence in insects and reduced the seed damage as well as weight loss of fumigated pigeon pea seeds up to 6 months of storage as compared to control set. Thus, Chenopodium oil can be used as an effective option of commercial fumigants for the storage of pigeon pea seeds against pulse bruchids.
ABSTRACT Essential oils isolated from 35 aromatic plants of Gorakhpur Division, India, were screened at 0.36 ml ml21 against four dominant fungi Aspergillus flavus Link, A. niger van Tieghem, A. ochraceus Wilhelm and A. terreus Thom found... more
ABSTRACT Essential oils isolated from 35 aromatic plants of Gorakhpur Division, India, were screened at 0.36 ml ml21 against four dominant fungi Aspergillus flavus Link, A. niger van Tieghem, A. ochraceus Wilhelm and A. terreus Thom found on stored pigeon pea (Cajanus cajan Linn.) seeds. Chenopodium ambrosioides Linn. and Citrus aurantium Linn. oils exhibited strong antimycotic activity (100% mycelial inhibition). The minimum inhibitory concentration of C. ambrosioides oil was less (0.07 ml ml21) than that of C. aurantium oil, against all the test fungi. Both the oils were fungicidal at higher doses (1.71–2.78 ml ml21). C. ambrosioides oil exhibited broad fungitoxic spectrum against 16 other storage fungi of pigeon pea seeds. The oil remained effective with increased inoculum load, and physical factors had no adverse effect on its toxicity. C. ambrosioides oil proved superior to aluminium phosphide and ethylene dibromide, synthetic fumigants, at 0.29 ml ml21 and significantly protected 1 kg of pigeon pea seeds from microbial attack for up to 6 months. Chenopodium oil can be recommended as an effective substitute of synthetic fumigants for storage of pigeon pea seeds.
From time immemorial, man has been in a continual fight with pests (Insects, plant disease, rodents, weeds etc.) that compete for his food supply, damage his forests and live stocks and is responsible for several diseases. The interest in... more
From time immemorial, man has been in a continual fight with pests (Insects, plant disease, rodents, weeds etc.) that compete for his food supply, damage his forests and live stocks and is responsible for several diseases. The interest in the use of essential oils for the pests and pathogens control is not new and originates from the need for pesticide production with eco-friendly, easily biodegradability and negligible health impacts than synthetic pesticides. These oils have a potential to be used as protectant of stored products including cereals, grains and pulses. Recently it has been reported that some chemical constituents derived from such essential oils also affect the nervous system of stored product insects. The expanding literature on the possibility of the use of these volatiles is reviewed in the present time. It focuses on the oil’s effect on oviposition, repellency, ovicidal, pupicidal, larvicidal, insecticidal and antifeedant activities of insects. Possible attracti...

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